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ACE inhibition in goats under fixed‐time artificial insemination protocol increases the pregnancy rate and twin births 
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下载免费PDF全文 VP Fernandes Neto MNN Silva AS Costa D Kunkel AS Júnior LCS Feitosa MCS Muratori APR Costa 《Reproduction in domestic animals》2018,53(4):1006-1008
To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology. 相似文献
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试验旨在建立高效的经产母猪定时输精（timed artificial insemination,TAI）技术,研究了定时输精对经产母猪繁殖性能、断奶-分娩间隔、不同胎次母猪产仔性能及断奶后7 d内血清生殖激素水平的影响。选取309头2~8胎次二元（长×大）经产母猪,随机分为对照组和试验组,对照组母猪进行常规人工授精（artificial insemination,AI）,试验组母猪进行断奶后24 h注射PMSG 1 000 IU,间隔72 h注射GnRH 100 μg,在注射GnRH后24和40 h各输精1次的定时输精技术。通过统计两组母猪的断奶1周内发情率、受胎率、分娩率、窝均产仔数等,判断定时输精对经产母猪繁殖性能的影响;通过对断奶时间和分娩时间的统计,检测定时输精对经产母猪断奶-分娩间隔的影响;用放射免疫（RIA）方法检测2~4胎次母猪断奶1周内血清E2、LH、FSH和P4的含量,研究定时输精对母猪生殖激素的影响。结果显示,试验组母猪发情率显著高于对照组（P<0.05）,但两组间受胎率、分娩率差异不显著（P>0.05）,窝均产仔数、窝均合格仔数和繁殖效率有增加的趋势,但差异不显著（P>0.05）;定时输精显著缩短了母猪的断奶-分娩间隔（P<0.05）。在胎次方面,3~4胎母猪使用定时输精的效果较好,其发情率、受胎率和分娩率均显著高于对照组（P<0.05）。在生殖激素方面,试验组E2水平在注射PMSG后迅速上升,且在定时输精处理后66~96 h内持续高于对照组（P<0.05）,试验组P4水平在断奶后至配种前显著低于对照组（P<0.05）,但配种后快速升高,并高于对照组;LH和FSH的含量在两组间无显著差异。综上,定时输精可有效提高经产母猪的断奶发情率,并减少其非生产天数,可显著提高3~4胎母猪的繁殖性能。 相似文献
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Cavestany D Betancour H Blanc JE Lemaire C Slavica J Moreira F Piaggio J Risco C 《Australian veterinary journal》2007,85(4):141-147
OBJECTIVE: To evaluate the effectiveness of a reproductive management program consisting of combinations of Ovsynch/TAI and prostaglandin (PG) F(2alpha) treatments in Holstein dairy cows under a pasture-based dairying system. DESIGN: Field trial. PROCEDURE: A total of 1177 cows in 8 commercial dairy farms were randomly allocated to control and treatment groups. Treatment group cows received one of two interventions depending upon the number of days postpartum (DPP) before the planned start of breeding. Cows more than 50 DPP by the planned start of breeding received the Ovsynch/TAI treatment, consisting of gonadotrophin releasing hormone (GnRH) - PGF(2alpha)- GnRH plus timed artificial insemination. Cows between 40 and 50 DPP received a PGF(2alpha) treatment followed by oestrus detection and, if the cow was not seen in oestrus, the cow received a second PGF(2alpha) 14 days later. Control cows were submitted to twice a day heat detection followed by artificial insemination. The experimental period was the start of the breeding season plus 21 days for cows over 50 DPP at the start of breeding, and was 40-61 DPP for cows that calved later and passed their voluntary waiting period after the start of the breeding season. RESULTS: Submission rate was higher for the treated group than for the control group (84.9% vs. 55.1%; P < 0.0001), as was the conception rate (51.0% vs. 46.1%; P < 0.03). Due to farm variations, pregnancy rate was similar in both groups (38.5% vs. 28.2%; P > 0.1). Within the treated group, conception rate and pregnancy rate of the cows inseminated after a PGF(2alpha) were higher than for timed artificial inseminated cows (51.4% vs. 32.6%; P < 0.001), and (37.8% vs. 32.6%; P < 0.001). CONCLUSION: A programmed reproductive management protocol may improve reproductive efficiency in dairy farms with seasonal breeding, by increasing submission and conception rates at the beginning of the breeding season and/or at the end of the voluntary waiting period. Fertility of cows bred after a PGF(2alpha) synchronised heat was greater than after an Ovsynch/TAI protocol. 相似文献
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2009年8月在青海省贵南县塔秀乡用Ovsynch/TAI模式处理母牦牛18头,按隔年产母牛和当年产母牛分为两组,用西门塔尔细管冻精进行人工授精,翌年5月6日开始生产,共生产犏牛3头,情期受胎率为16.67%,总受胎率为66.67%。 相似文献
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Nilson Shonholzer Faleiro Kedson Alessandri Lobo Neves Luciano Leite Pereira Cleidson Manoel Gomes da Silva William Gomes Vale Antonio Humberto Hamad Minervino 《Reproduction in domestic animals》2019,54(7):1050-1053
The aim of this study was to develop a resynchronization strategy before the return of oestrus in cows diagnosed as not pregnant after fixed‐time artificial insemination (TAI). A total of 839 cows, approximately 45 days post‐partum, were synchronized using TAI. On day 0, intravaginal progesterone‐releasing devices were inserted and 2 mg of oestradiol benzoate was administered. Eight days later (D8), the progesterone‐releasing devices were removed and oestradiol cypionate (0.5 mg, eCG [300 IU]) and prostaglandin (7.5 mg) were administered. All cows were inseminated between 48 and 56 hr after device removal (D10). Thirty days after TAI, cows that were not diagnosed as pregnant by ultrasound were immediately resynchronized and again inseminated at a fixed time. The hormonal protocol used in the first and second rounds of TAI was the same. The pregnancy rate after the first TAI was 52%, and after the second TAI, it was 49%. The increase in the total pregnancy rate (synchronization + second oestrous synchronization) compared to a single synchronization was 23.5%. In conclusion, resynchronization of oestrus and ovulation in zebu cows that had previously undergone TAI protocols is effective in increasing the reproductive efficiency. 相似文献
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WU Jun-hui CAO Ding-ren LIU Yan-ting GAO Feng-lei LI Li WU Tong-shan ZHANG Shou-quan WEI Heng-xi 《中国畜牧兽医》2016,43(7):1826-1833
With the wide application of the artificial insemination (AI) in animal husbandry,the utilization rate of excellent sire got greatly improved.The key point of AI is predicting the time of ovulation and insemination accurately.It is also the point which the animal husbandry technologists highly concerned to improve the application effect of AI.Estrus and ovulation are regulated minutely by reproductive hormones in vivo in farm animals.Timed artificial insemination (TAI) is based on the synchronization of estrus and ovulation,both of which are triggered by reproductive hormones.So the optimal time for AI could be determined from the reproductive hormone treatment.The purpose of TAI is to improve the reproduction performance of farm animals,reduce the workload of identification of estrus,and promote batch management.This review mainly summerized the principles of TAI and its applications status,and the prospects was also discussed.It would provide a reference for the research and application of new reproductive technology in farm animals. 相似文献
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随着人工授精技术在畜牧业中的广泛应用,优秀种公畜的利用率得到了极大提高。而人工授精成功的关键是输精的准确性和及时性,因此如何准确预测排卵时间及输精时间是提高人工授精效果的重要途径,也是当前畜牧工作者高度关注的问题。家畜的发情排卵受到体内生殖激素的精密调控,定时输精技术就是在利用生殖激素进行同期发情、同期排卵的基础上,通过调控母畜的发情和排卵来确定人工输精的最佳时间,以期达到提高家畜繁殖性能、减少发情鉴定工作量和促进养殖场批次化管理的目的。作者综述了家畜定时输精的原理及当前的应用现状,同时对其发展前景进行了展望,以期为畜牧业繁殖新技术的研究和应用提供参考依据。 相似文献
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