THE INSENSITIVITY OF ^99mTc PERTECHNETATE FOR DETECTING METASTASES OF A FUNCTIONAL THYROID CARCINOMA IN A DOG     

Michael R.  Broome  DVM  MS  Gayle S.  Donner  DVM 《Veterinary radiology & ultrasound》1993,34(2):118-124

This report describes the use of ^99mtechnetium pertechnetate (^99mTcO₄⁻) and ¹³¹[for imaging of a metastatic thyroid carcinoma in a dog. The ¹³¹] imaging showed metastatic lesions undetected by the ^99mTcO₄⁻ imaging on 2 separate occasions. The possible mechanisms for the discrepancies between ¹³¹I and ^99mTcO₄⁻ imaging of thyroid carcinomas are discussed. The use of ¹³¹I for the imaging of functional thyroid carcinomas in the dog is recommended.  相似文献   

THE NON-CARDIAC DISTRIBUTION OF ^99mTC-METHOXY-ISOBUTYL-ISONITRILE (SESTAMIBI) IN NORMAL BEAGLES     

Phillip F.  Steyn  BVSc  MRCVS  MS  Gregory  Ogilvie  DVM 《Veterinary radiology & ultrasound》1995,36(2):160-163

Technetium-99m methoxy-isobutyl-isonitrile (sestamibi) is a radiophartnaceutical used for the evaluation of myocardial perfusion. Increased uptake of sestamibi has also been documented in tumors. The objective of this study was to document the extracardiac biodistribution of ^99mTc sestamibi in the normal dog. Nine normal beagles were given 0.35 mCi/kg ^99mTc sestamibi intravenously, and 60 second images were made of the entire body at 1 hour post injection. A defined distribution pattern was recognized, with good visualization of the heart, liver, gallbladder, small intestine, kidneys, urinary bladder, popliteal lymph nodes, parotid salivary glands and zygomatic salivary glands. Splenic uptake was not seen.
Target to background ratios were calculated for all the regions listed, using background regions-of-interest with the smallest coefficient of variance for the denominator. The mean, range and standard deviation of these ratios are given.  相似文献   

Different biological behaviour of Waldenström macroglobulinemia in two dogs     

F. Gentilini  C. Calzolari  A. Buonacucina  M. Di Tommaso  G. Militerno  P. Famigli Bergamini 《Veterinary and comparative oncology》2005,3(2):87-97

Waldenström Macroglobulinemia is a low‐grade immunosecretory disorder associated with lymphoid tumours, which is rarely reported in veterinary medicine. In this study, we describe two clinical cases of this rare syndrome in dogs, each characterized by a different onset and clinical course. In one case, a hyperacute onset and aggressive behaviour of the neoplasm was observed. Absolute serum viscosity (SV) was retrospectively evaluated in order to explain clinical findings. Rotational viscosimetry showed good precision in measuring SV. Both dogs had SV values higher than a control groups of healthy dogs although only one subject developed hyperviscosity symptoms and complications. At high paraprotein concentrations, a slight reduction of the M‐component was associated with a marked decrease in SV. Thus, this work suggests that SV assessment is a relevant tool for managing monoclonal gammopathies, whose usefulness should be further confirmed in larger cohorts of dogs.  相似文献   

Diagnosis of Congenital Cardiac Right-to-left Shunts with ^99mTc-macroaggregated Albumin     

Federica Morandi  DVM  MS    Gregory B. Daniel  DVM  MS    Rebecca E. Gompf  DVM  MS    Anne Bahr  DVM  MS 《Veterinary radiology & ultrasound》2004,45(2):97-102

Diagnosing right-to-left congenital cardiac shunts can be difficult. Cardiac catheterization and angiocardiography represent the traditional gold standard for diagnosis, but they are invasive. Nuclear scintigraphy using 99mTc-macroaggregated albumin (MAA) has been employed in humans as an alternate method of diagnosis. This study reviews eight dogs presented for evaluation of a suspect right-to-left cardiac shunt that were examined using 99mTc-MAA. In all, 2-4 mCi (74-148 MBq) of reduced particle 99mTc-MAA were injected IV in a cephalic vein and static images of the whole body, including right and left lateral, dorsal, and ventral views, were acquired for 60 s and stored into a 256 x 256 x 16 matrix. Shunt fractions were calculated. One dog with radiopharmaceutical distribution limited to the lungs did not have a shunt. Seven dogs had distribution of the radiopharmaceutical outside the pulmonary capillary bed, indicating bypassing of the pulmonary capillary circulation due to a right-to-left shunt. Four dogs had 99mTc-MAA within the brain. Three dogs that did not have brain uptake, but instead had a sharp cutoff of radioactivity at the level of the front limbs and neck, were diagnosed with reverse patent ductus arteriosus (PDA). The asymmetric distribution of the radiopharmaceutical is due to the location of the shunt, distal to the brachiocephalic trunk and left subclavian artery. Shunt fractions of dogs with extrapulmonary radioactivity ranged from 40% to 59%. Nuclear scintigraphy with 99mTc-MAA is a quick alternative method of diagnosing right-to-left cardiac shunts that permits quantification of shunt fraction. Distinguishing between reverse PDA and other right-to-left shunts may be possible based on the radiopharmaceutical distribution.  相似文献   

m6A甲基化在鸡肌肉生长发育中的表达研究     

丁浩  林月月  张涛  张闪闪  吴玉麟  段严军  巩用双  谢恺舟  王金玉  戴国俊  张跟喜 《中国畜牧兽医》2021,48(5):1525-1534

试验旨在研究RNA m6A修饰相关基因去甲基化酶Alk B同源蛋白5（Alk B homologue 5，ALKBH5）、去甲基化酶肥胖相关蛋白（fat mass and obesity-associated protein，FTO）、甲基转移酶样蛋白3（methyltransferase like 3，METTL3）、甲基转移酶样蛋白14（methyltransferase like 14，METTL14）和成肾细胞瘤1-结合蛋白（Wilms’tumor 1-associating protein，WTAP）在鸡骨骼肌发育过程中的表达，分析其与骨骼肌m6A甲基化水平的相关性。首先，利用实时荧光定量PCR技术检测m6A甲基化相关基因在金茅花鸡12（E12）、14（E14）、16（E16）、18（E18）胚龄和1日龄腿肌和胸肌组织中mRNA表达水平，以及其在鸡成肌细胞50%、100%增殖期和1、2、3、4、5 d分化期的mRNA表达水平；随后，利用m6A甲基化试剂盒检测金茅花鸡E12和1日龄腿肌和胸肌组织中m6A甲基化修饰水平，与m6A甲基化相关基因表达水平进行相关性分析。结果显示，m6A去甲基化基因ALKBH5和FTO mRNA表达水平在骨骼肌发育过程中显著上调（P<0.05），即在E12、E14低表达，E16、E18逐渐上调，1日龄达到最高。m6A甲基化写入基因METTL14、METTL3和WTAP mRNA表达水平在E12、E14、E16逐渐上升，E18下降，随后至1日龄表达量回升。在细胞增殖过程中，ALKBH5、FTO、METTL14、METTL3和WTAP基因表达均上调；在细胞分化过程中ALKBH5和FTO基因表达水平显著上调（P<0.05），在分化第5天达到最高。METTL14、METTL3和WTAP基因mRNA表达水平在细胞诱导分化的1、2、3、4 d表达量呈下降趋势，而在诱导分化的第5天有所回升。甲基化水平检测结果显示，腿肌和胸肌m6A甲基化水平变化趋势一致，均在胚胎发育过程中显著下降（P<0.05），至1日龄达到最低。相关性分析结果显示，鸡骨骼肌RNA m6A甲基化水平与m6A去甲基化修饰基因ALKBH5、FTO mRNA表达水平呈显著负相关（P<0.05）。综合以上试验结果，推测m6A甲基化修饰与鸡骨骼肌发育相关，而去甲基化基因ALKBH5、FTO可能通过调控RNA m6A甲基化水平，影响鸡骨骼肌发育。本研究结果为进一步研究m6A甲基化修饰调控鸡骨骼肌生长发育的功能和分子机制提供理论依据。  相似文献   

利用农田表层(0～20cm)有机碳估算1m土体有机碳的可靠性   总被引：1，自引：0，他引：1  

马渝欣  李德成  李徐生  张甘霖  韩志勇  李贤胜  杨平  何小卫  赵燕洲  李若清  奎秀 《土壤学报》2014,51(1):189-193

<正>土壤有机碳(SOC)是近年来全球变化研究的热点之一,表层(0~20cm)和1m土体的SOC是评估农田SOC的两个重要指标[1]。很多研究利用第二次土壤普查资料来估算农田SOC[2-6],一些研究已表明近20年来我国耕作土壤,尤其是表层(一般为0~20cm)的SOC基本呈增加的趋势[5-8],这与秸秆还田、有机肥与化肥合理施用以及少(免)耕技术推广有关[9-10]。但由于农田SOC,至少是表层SOC,是  相似文献   

基于二分法的微灌毛管水力设计   总被引：2，自引：0，他引：2  

王新坤 《排灌机械》2007,25(6):27-30

根据微灌毛管水力学特性,采用二分法搜索原理,并与逆递推法相结合,提出微灌毛管水力解析与设计方法。利用计算机编程从毛管末端向毛管进口逆序递推,可以快速、方便地求解出毛管各个孔口的压力和流量,设计毛管运行的水力参数,校核平均流量、流量偏差率等设计控制指标。实例验证结果表明,该设计方法具有直观、简便和快捷的特点,易为非专业人员理解与应用,具有较高的求解效率和计算精度。  相似文献   

3个豇豆株系的性状观察比较试验     

彭友林  王云 《长江蔬菜》2009,(22)

对豇豆99-2,99-3,99-4三个优良株系性状进行比较试验.结果表明,豇豆99-2具有生长势好、早熟、高产、优质、商品性好等特点,丰产性尤为突出;前期产量12 304.5 kg/hm2,占总产量33 949.5 kg/hm2的36.68%,鲜荚比99-3、99-4早2～3 d上市.可作为早熟品种推广栽培.  相似文献   

大肠埃希氏菌表面菌毛黏附因子多价菌毛疫苗的研制     

李鹏  王家乡  程碧军 《湖北农业科学》2006,45(5):643-646

为了预防仔猪黄痢,选取ETEC野生分离菌株HN2001(K88ab)、HN2002(K88ac)、HN2003(K88ad)、HN2004(K99)、HN2005(987p)、HN2006(F41)培养后用低温磁力搅拌法提取到菌毛,制成5批多价菌毛混合油乳剂灭活苗,实验室试验结果表明,5批多价灭活疫苗对小白鼠的平均保护率达95.0%,为进一步进行田间试验提供了理论依据,并将为生产上防治仔猪黄痢提供一种保护范围更加全面的生物制品.  相似文献   

高产高蛋白食饲两用早籼99早677的选育及其栽培技术     

郑瑞丰  曾德洪  李茂扬 《湖南农业科学》2006,(1):15-16

99早677系湘早籼19号与湘早籼24号配组杂交,经9 a选育而成,2004年2月通过湖南省农作物品种审定委员会审定命名.该品种全生育期110 d左右,一般产量7.2～7.6 t/hm2,抗白叶枯病,适合湖南全省各地作食饲两用早稻栽培.栽培技术要点密度13.3 cm×20.0 cm,全生育期施纯氮180 kg/hm2,氮磷钾比例为111.2.  相似文献