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1.
This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites, a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect, vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems. The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province. Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain Forest Ecosystem. Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China Responsible editor: Zhu Hong  相似文献   
2.
绵羊角的有无是由或主要由一个基因位点(Locus)上的三个等位基因(H、h’、h)所决定。三个等位基因的遗传方式是:H为显性无角基因,对h为完全显性,即Hh基因型均表现无角性状;h为隐性有角基因,即hh基因型均表现有角性状;h,为一个频率很低的基因,对h为不完全显性,即h'h基因型仍表现为有角性状;但H对h’基因为不完全显性,即Hh’基因型可能出现角痕(角突或角基)。而h’h’基因型公羊表现为小角、发育残缺不全的矫型角,母羊表现为无角。培育无角革的关键是选择使用无角公羊,通过测交试验能确定无角公羊基因的纯合度,从而决定如何使用。  相似文献   
3.
Type III procollagen peptide (P-3-P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P-3-P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P-3-P, followed by Western immunoblotting with rabbit aniti-P-3-P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P-3-P and sera from two dogs suspected of having elevated P-3-P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross-reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P-3-P. All tested sera from dogs had minimal competitive binding with radiolabeled P-3-P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P-3-P concentrations in any of the groups of dogs studied. It was concluded that currently available radioimmunoassay kits for the measurement of P-3-P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P-3-P may vary significantly from that of the bovine or human, limiting the sensitivity and specificity of this assay in the dog.  相似文献   
4.
梁泽胜 《安徽农业科学》2011,39(27):16757-16758
从青海省都兰县基本情况出发,介绍了都兰县盐生植物的分布和盐生植物资源种类,并阐述了其盐生植物资源的利用情况。  相似文献   
5.
AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.  相似文献   
6.
7.
AIM To investigate whether microRNA-9-5p (miR-9-5p) mediates sympathetic overactivity by targeting KCNN3 (potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3) gene,which encoded small-conductance calcium-activated potassium channel 3 (SK3) protein, in paraventricular nucleus (PVN) of rats with type 2 diabetes mellitus (T2D). METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus (rAAV)-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control (DC) group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus (DM) rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced (P<0.05). However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.  相似文献   
8.
通过对湿热地区云南河口县芒果园内的5种主要病虫害发生规律进行调查,根据调查情况对芒果白粉病、炭疽病,芒果横线尾夜蛾、芒果切叶象甲、桔小实蝇等5种发生程度较重的病虫害进行发生危害情况的相关分析,同时对云南河口地区芒果害虫天敌进行搜集、调查及鉴定,调查鉴定结果表明:共有天敌昆虫24种,其中寄生性天敌11种,分属于2目5科;捕食性天敌昆虫21种,分属于5目8科。调查结果对于生产上科学制定芒果病虫害防治措施具有重要的意义。  相似文献   
9.
秸秆还田种类对稻田N2O排放及硝化反硝化微生物的影响   总被引:1,自引:0,他引:1  
以太湖流域典型单季稻田的原状土柱为研究对象,通过设置温室土柱试验,同步监测3种秸秆(水稻秸秆RS、小麦秸秆WS、玉米秸秆MS)施用下水稻各生长期N2O排放、水稻产量和土壤理化因子,同时定量化分析多个N2O排放相关菌群及功能基因的丰度,以阐明N2O排放对不同种类秸秆施用引发的微生物响应机制,筛选控制单季稻田N2O减排增效最佳的秸秆种类。结果表明:与对照相比,RS、WS和MS处理下水稻生长期N2O排放量分别增加162.32%、107.11%和9.48%,其中RS处理显著高于MS处理。水稻生育期内,土壤氨氧化菌(AOA、AOB)和反硝化菌群落(nir S、nos Z)丰度均呈现先上升后下降的变化趋势。与对照相比,拔节期RS处理显著增加AOA、AOB、nirS和nosZ拷贝数,MS和WS处理对上述功能基因丰度均无显著影响。各生育期土壤NH4+-N含量整体高于NO3-N含量,二者均在水稻分蘖期达到峰...  相似文献   
10.
为了研究黄淮海平原不同秸秆还田方式和施氮类型对夏玉米农田生态系统土壤呼吸的影响,于2010年6—10月,采用LI-COR-6400-09土壤气室连接红外线气体分析仪(IRGA)对玉米农田行间掩埋秸秆区的土壤呼吸作用进行了连续测定。结果表明,常规施肥下,玉米生育期内秸秆行间掩埋处理(ISFR)的平均土壤呼吸速率显著高于秸秆移除(NSFR)和秸秆覆盖(SFR)处理(P<0.05)。秸秆行间掩埋配合施用化学氮肥处理中,配施50.4 kg(N).hm 2处理(ISF3)的平均土壤呼吸速率为(178.85±46.60)mg(C).m 2.h 1,显著高于配施33.6 kg(N).hm 2处理(ISF2)的(124.11±23.18)mg(C).m 2.h 1(P<0.05)。秸秆行间掩埋配合施用鸡粪处理中,鸡粪施用量为33.6kg(N).hm 2(ISOM2)处理的平均土壤呼吸速率为(208.08±31.54)mg(C).m 2.h 1,施用16.8 kg(N).hm 2(ISOM1)和50.4 kg(N).hm 2(ISOM3)处理的为(135.07±21.97)mg(C).m 2.h 1、(171.43±43.31)mg(C).m 2.h 1,相比ISOM2处理,ISOM1和ISOM3处理的平均土壤呼吸速率降低了35.09%和17.61%。ISOM2处理玉米季CO2排放累积量为499.39 g(C).m 2,显著高于ISF2处理的297.86 g(C).m 2。秸秆行间掩埋配合施用化学氮肥对土壤呼吸速率的影响小于配合施用鸡粪的影响,配合施用16%总氮的鸡粪,即33.6 kg(N).hm 2时C/N比最适宜土壤微生物的代谢活动。  相似文献   
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