Early pregnancy alters the metabolic responses to restricted nutrition in sheep     

Sosa C  Abecia JA  Carriquiry M  Forcada F  Martin GB  Palacín I  Meikle A 《Domestic animal endocrinology》2009,36(1):13-23

This study investigated whether a 27-day period of nutrition at half-maintenance during early pregnancy (up to Day 14) could alter maternal endocrine responses. Forty-six ewes were fed all or half of their maintenance requirements and slaughtered on Day 14 of the oestrous cycle or pregnancy. We used real time RT-PCR to study gene expression of growth hormone receptor (GHR) and leptin in adipose tissue and GHR, GHR1A and of the insulin-like growth factor I (IGF-I) in the liver. Blood profiles of metabolites and metabolic hormones were also determined. Throughout the experiment, underfed animals presented lower body weight and body condition, greater plasma concentrations of non-esterified fatty acids (NEFA), and lower plasma concentrations of leptin, compared to adequately fed animals. Undernutrition affected the patterns of gene expression in adipose and hepatic tissues, and the responses differed between pregnant and non-pregnant ewes. In adequately fed ewes, pregnancy up-regulated leptin mRNA expression in adipose tissue, a response that was impaired in underfed ewes. The hepatic expression of IGF-I mRNA was increased by pregnancy in underfed animals while no effect was observed in adequately fed ewes. It remains to be determined whether the changes in the endocrine milieu are paralleled by modifications in uterine gene expression that could alter the environment of the embryo during early pregnancy.  相似文献   

妊娠后期不同营养水平对蒙古绵羊胎儿生长发育的影响   总被引：5，自引：1，他引：5  

高峰  侯先志 《中国农业科学》2007,40(6):1260-1264

 【目的】研究妊娠后期不同营养水平限制饲养母羊对其胎儿生长发育的影响。【方法】选择经同期发情且同期受孕, 在妊娠90 d时健康的蒙古绵羊100只，ME分为0.175MJ•kg-1w0.75•d-1（RG1）、0.33 MJ•kg-1w0.75•d-1（RG2）以及自由采食组(CG)3个营养水平处理组，进行不同营养水平限制饲养。【结果】妊娠后期不同营养水平限饲母羊，严重限制了RG2（P＜0.05）、RG1（P＜0.01）两低营养组羔羊平均初生重及胎儿期平均日增重；限制了RG1组初生羔羊体长（P＜0.01）、胸围（P＜0.01）、肺脏（P＜0.01）、脾脏（P＜0.01）、心脏（P＜0.05）、肝脏（P＜0.05）及皱胃重（P＜0.01）。RG2组的肺脏（P＜0.05）、脾脏重（P＜0.01）表现出与对照组（CG）明显的差异，其它组织器官重尽管都不同程度的表现出降低的趋势，但与对照组差异不显著（P＞0.05）。各组织器官重占体重百分比，两限制组脾脏仍比对照组显著低（P＜0.01）。脑占体重的比例，对照组显著低于RG1组（P＜0.01）。【结论】妊娠后期不同营养水平限制饲养母羊显著影响了胎儿生长及器官发育，而且随着营养水平的降低，受限制的程度也逐步加深。  相似文献