马尾松叶表微生物多样性     

袁贵云  孙学广  郑炀  郭其强  丁贵杰 《森林与环境学报》2021,(3):318-324

为发掘对林木生长发育有利的优良微生物资源,并筛选适合叶表微生物的收集方法,以马尾松针叶为试验材料,分别用悬摇法和超声波法收集马尾松叶表微生物,用扩增子高通量测序技术、MUSCLE和Qiime软件研究马尾松叶表微生物的多样性。结果表明:扫描电镜观测结果显示,马尾松针叶表面定殖有大量微生物,包括真菌(菌丝及孢子)和细菌。扩增子高通量测序结果表明,马尾松叶表微生物物种丰富,包含细菌运算分类单位(OTUs)490个,真菌OTUs 1273个。马尾松叶表细菌以未分类的蓝细菌属(unidentified_Cyanobacteria)(36.53%)、未分类的拜叶林克氏菌属(unidentified_Beijerinckia)(28.60%)、鞘氨醇单胞菌属(Sphingomonas)(2.35%)为优势属;叶表真菌以枝孢属(Cladosporium)(2.45%)、拟盘多毛孢属(Pestalotiopsis)(0.92%)、无头孢菌属(Capnobotryella)(0.91%)为优势属。针对叶表细菌多样性的研究表明,悬摇法和超声波法均有较高的物种检出度;在叶表真菌多样性的研究中超声波法优于悬摇法,但超声波法样品间数据变异性较大,测定结果不稳定。  相似文献   

Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust,Locusta migratoria          下载免费PDF全文

Xiao-ming ZHAO  Jia-peng YANG  Xin GOU  Wei-min LIU  Jian-zhen ZHANG 《农业科学学报》2021,20(6):1596-1606

Cuticular proteins(CPs) are major components of the insect cuticle-associated organs such as integument and wings, although the importance of CPs for wing development and function in hemimetabolous insects remains understudied. In the present study, a wing cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to the RR-2 subfamily of cuticular protein RR consensus(CPR) chitin-binding proteins. LmACP8 was mainly expressed in the wing pads and showed high expression levels before ecdysis of third-, fourth-, and fifth-instar nymphs, with its encoded protein located in the procuticle of wing pads and adult wings. Depletion of LmACP8 by RNA interference markedly reduced the amount of its protein, which consequently caused abnormal wing morphogenesis in the transition from nymph to adult of L. migratoria. We further demonstrated that the abnormal morphogenesis was caused by severe damage of the endocuticle in the wings. LmACP8 was suppressed by 20-hydroxyecdysone(20 E) in vivo, however, its expression was significantly up-regulated after knocking down the hormone receptor gene LmHR39. Thus, the LmACP8 that is negatively regulated by the LmHR39-mediated 20 E signaling pathway is involved in wing development during the nymph to adult transition.  相似文献   

菠萝蜜种子淀粉制备香草兰微胶囊的工艺研究     

张彦军  朱红梅  田建文  徐飞  初众 《热带作物学报》2017,38(6):1127-1133

以菠萝蜜种子淀粉为壁材,采用饱和水溶液法制备香草兰精油微胶囊,以包埋产率为指标,采用响应面分析法对微胶囊的包埋条件进行优化探讨。结果表明:5个单因素中,影响最显著的因素为壁芯材比例、包埋温度和包埋时间。响应面优化得到香草兰精油微胶囊的最佳工艺条件为壁芯材比例为7.5∶1;包埋时间72 min;包埋温度54℃,此条件下的包埋产率为(95.46±0.2)%,包埋率为(76.35±0.6)%,载油量为(27.73±0.3)%。试验证明,此条件结果与模型预测值相吻合,此工艺条件可为菠萝蜜种子淀粉包埋香草兰精油微胶囊工艺提供理论依据。  相似文献   

Replacing Soybean Meal with Alternative Protein Sources in Diets for Pond‐raised Hybrid Catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus          下载免费PDF全文

Menghe H. Li  Brian G. Bosworth  Penelope M. Lucas 《Journal of the World Aquaculture Society》2018,49(4):755-760

The present study investigated the replacement of soybean meal with combinations of two or three alternative protein sources in diets for pond‐raised hybrid catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus. Alternative protein sources evaluated included cottonseed meal, distillers dried grains with solubles (DDGS), peanut meal, and porcine meat and bone meal (PMBM). Hybrid catfish fingerlings with a mean initial weight of 35 g/fish were stocked into 25 earthen ponds (0.04 ha) at a density of 14,826 fish/ha. Fish were fed once daily to apparent satiation for 166 d. No significant differences were observed for total diet fed, net yield, weight gain, survival, carcass yield, fillet yield, or fillet proximate composition among dietary treatments. Results show soybean meal may be completely replaced by combinations of cottonseed meal and one or two other alternative protein sources including DDGS, peanut meal, and PMBM in the diet without markedly affecting production and processing characteristics and fillet proximate composition of pond‐raised hybrid catfish. These alternative diets may be used during foodfish production when prices are favorable.  相似文献   

An investigation on the effect of heat-moisture treatment on baking quality of wheat by using response surface methodology     

《Journal of Cereal Science》2017

Effect of heat-moisture treatment on quality properties of two bread wheats (cvs. Tosunbey and Bayraktar) were investigated by using response surface methodology (RSM). Temperature and moisture conditions in the experimental design were in the range of 55–95 °C and 13–19%. Heat-moisture treated grains were milled into flour and quality properties were determined. The optimum moisture-temperature combination for the highest dry gluten, Zeleny sedimentation, Alveograph W and bread volume values were estimated as 14%-63 °C for Tosunbey and 19%-55 °C for Bayraktar samples. Alveograph W seems to be a good indicator of baking quality for wheats treated at higher temperatures. In order to describe the relationship between the dependent and independent variables (moisture, temperature), the response values were fitted by second order polynomial models. Significance analysis showed that the effect of both moisture and temperature on dry gluten content, sedimentation and falling number values for Tosunbey; falling number and damaged starch values for Bayraktar were significant (p < 0.05). The effect of temperature on Farinograph water absorption, W and P/G, bread volume and firmness values were significant for both cultivars (p < 0.05). It can be concluded that improvement in baking quality can be achieved and flours with different properties can be produced by heat-moisture treatments on wheat.  相似文献   

橡胶园酸性、中性土壤交换性钙、镁测定方法研究     

许木果  丁华平  刘忠妹  陈桂良  陈永川  黎小清 《中国农学通报》2020,36(18):73-79

为了获得简单、易操作、准确度高的橡胶园酸性、中性土壤交换性钙、镁测定方法,本试验通过响应面优化试验,建立称样量、提取液体积、提取方式与交换性钙测定结果之间的数学模型。结果表明：在25℃条件下,以1 mol/L的乙酸铵为土壤浸提液,称样量为2 g、提取液体积175 mL、振荡36 min,为橡胶园酸性、中性土壤的最佳提取条件。将上述条件下得到的结果与标准方法条件下得到的结果相对比,两结果的相对误差在1.25~3.04之间,均属合理范围。利用标准样品对优化方法进行试验验证,结果与推荐值相符。改进后的方法分析成本低、操作简便、测定结果准确、稳定性好,可用于橡胶园酸性、中性土壤交换性钙、镁的测定。  相似文献   

群钻钻削精度试验     

王志安  胡仲勋 《湖南农业大学学报(自然科学版)》1996,23(4)

通过群钻钻头直径Ｄ，进给量ｆ和切削速度ｎ对孔径扩张量，圆度，孔中心线垂直度和直线度影响的试验，得出它们的变化规律。  相似文献   

氮吸附法分析生物质焦孔隙结构     

林晓芬  张军  尹艳山  盛昌栋 《福建农业学报》2009,24(3):262-265

采用氮吸附法对4种生物质焦(稻壳、树叶、玉米秆、棉花秆)的孔隙结构进行测量,结果表明,不同种类焦样的比表面积和孔径分布有明显差别,树叶的比表面积最大,为242.21 m2·g-1,玉米秆的比表面积最小,为0.81 m2·g-1.850℃时,稻壳、树叶、玉米秆焦样的孔径分布曲线在微孔和中孔范围各有一个分布峰,而棉花秆焦样的孔径分布曲线只在中孔范围内出现一个分布峰.热解温度是影响孔隙结构的一个重要因素,在高温条件下,同步热解得到的焦样的比表面积较大,微孔较多.在本研究中,600℃、850℃的稻壳焦样和850℃的树叶焦样具有较大的比表面积,比较适合做吸附剂.  相似文献   

猪肺炎支原体及其生物学研究进展   总被引：10，自引：0，他引：10  

李彦明  张映 《动物医学进展》2003,24(3):25-27

猪肺炎支原体是引起猪支气管肺炎的主要病原 ,严重危害着养猪事业的发展。虽然猪肺炎支原体的结构简单 ,但由于它对营养要求较高 ,故培养难度大 ,也是人们不能对其深入研究的一个主要原因。近年来利用克隆技术 ,对猪肺炎支原体从基因水平上进行了多种研究 ,已经取得了一些可喜成果。文章从它的生物学特性、荚膜结构、膜蛋白研究以及基因水平的研究等方面进行了综合性论述 ,并简明地指出了目前研究中存在的问题以及未来展望  相似文献   

蛋白质着色动力学研究     

文泽富  陈宗道 《西南大学学报(自然科学版)》1994,16(3):190-193

经过蛋白质着色动力学的研究，发现蛋白质着色的行为遵循数学模型Ｙ＝ａＸ＾ｂ。在ｐＨ２－６范围内，蛋白质上染料随染溶ｐＨ的下降而上升，在１５－３０℃温度范围内，蛋白质上染率随温度上升略有提高，当温度超过３０℃时，蛋白质上染率逐渐下降。染浴中添加稀土对蛋白质有较强的促染效果，其实质是染浴中蛋白质首先对稀土高价阳离子进行结合，再通过高价阳离子对色素进吸附。  相似文献