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Bratislav Dejanovic Ivana Stevanovic Milica Ninkovic Ivana Stojanovic Irena Lavrnja Tatjana Radicevic Milos Pavlovic 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):53-61
This study was conducted to investigate whether agmatine (AGM) provides protection against oxidative stress induced by treatment with chlorpromazine (CPZ) in Wistar rats. In addition, the role of reactive oxygen species and efficiency of antioxidant protection in the brain homogenates of forebrain cortexes prepared 48 h after treatment were investigated. Chlorpromazine was applied intraperitoneally (i.p.) in single dose of 38.7 mg/kg body weight (BW) The second group was treated with both CPZ and AGM (75 mg/kg BW). The control group was treated with 0.9% saline solution in the same manner. All tested compounds were administered i.p. in a single dose. Rats were sacrificed by decapitation 48 h after treatment Treatment with AGM significantly attenuated the oxidative stress parameters and restored antioxidant capacity in the forebrain cortex. The data indicated that i.p. administered AGM exerted antioxidant action in CPZ-treated animals. Moreover, reactive astrocytes and microglia may contribute to secondary nerve-cell damage and participate in the balance of destructive vs. protective actions involved in the pathogenesis after poisoning. 相似文献
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Seedlings of four rice cultivars differing in salt tolerance were raised in sand cultures under increasing levels of NaCl salinity and metabolic levels of total polyamines, spermidine, spermine, agmatine and the diamine putrescine were determined in roots and shoots during 5-20 days growth period. Salinity caused a remarkable increase in total polyamines level in rice seedlings. At similar level of salinity roots as well as shoots of salt sensitive cvs. Ratna and Jaya showed higher level of total polyamines than tolerants. Salinity of 14 dSm−1 NaCl caused more than 2 times polyamine level in shoots of sensitive cultivars compared to tolerants. Accumulation of polyamines was greater in salt stressed shoots than roots. In nonsalinized seedlings there appeared a gradual increase in putrescine level during 5 to 20 days growth period. Salt treatment caused sharp increase in putrescine level in all cultivars, however under similar level of salinization salt stressed seedlings of sensitive cultivars had higher putrescine level than tolerants. In nonsalinized seedlings of sensitive cultivars spermidine level increased gradually during 5 to 20 days growth period whereas a decline in the level was observed in seedlings of tolerant cultivars during this period. Higher level of salinity caused marked increase in spermidine level in sensitive cultivars. Specially during 5 to 10 days of growth salinity caused increase in spermine level in seedlings of sensitive cultivars. In all cultivars salt stressed seedlings had higher agmatine level compared to non-stressed. Salinity led to greater accumulation of certain unidentified polyamines in seedlings of sensitive cultivars. Increased levels of total polyamines, putrescine, spermidine and unknown polyamines in rice seedlings under salinization suggest their possible role in combating the adverse effects of salinity stress. 相似文献
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嗜水气单胞菌是水环境常见的病原体,具有较强致病性。在本研究中,首次敲除了嗜水气单胞菌aguA基因,通过转录组分析发现aguA敲除后细菌的粘附、致病、胺代谢等生物过程下降。通过qRT-PCR进一步验证发现10个粘附基因、9个发病基因和8个胺代谢基因的mRNA表达水平明显下降；此外,转录组水平分析结合qRT-PCR验证发现VI型分泌系统基因、II型分泌系统基因、鞭毛合成相关基因、菌毛合成相关基因、溶血基因、I型分泌系统基因、肠毒素基因、RTX基因等毒力基因的RNA表达水平也出现下调。对两株菌进行草金鱼的体内感染实验,与野生菌株相比,aguA敲除株感染草金鱼的致病力降低了7.47倍,且aguA敲除株生物膜形成能力降低了16.6%。以上结果显示aguA基因与嗜水气单胞菌的致病性密切相关。 相似文献
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