Pharmacokinetics and Metabolism Study of Deep-Sea-Derived Butyrolactone I in Rats by UHPLC–MS/MS and UHPLC–Q-TOF-MS     

Liang Wu  Chun-Lan Xie  Xian-Wen Yang  Gang Chen 《Marine drugs》2022,20(1)

Butyrolactone I (BTL-I) is a butanolide isolated from the deep-sea-derived fungus, Aspergillus sp. It provides a potential new target for the prevention and treatment of food allergies. This study aimed to investigate the metabolic and pharmacokinetic profile of BTL-I in rats. The metabolic profiles were obtained by UHPLC–Q-TOF-MS. As a result, eleven metabolites were structurally identified, and the proposed metabolic pathways of BTL-I were characterized. The main metabolites were the oxidative and glucuronidative metabolites. In addition, a sensitive UHPLC–MS/MS method was established for the quantitation of BTL-I in rat plasma (LOQ = 2 ng/mL). The method was fully validated and successfully applied to the pharmacokinetic study of BTL-I in rats after oral administration or intravenous administration. The oral bioavailability was calculated as 6.29%, and the maximum plasma concentrations were 9.85 ± 1.54 ng/mL and 17.97 ± 1.36 ng/mL for intravenous and intragastric dosing groups, respectively.  相似文献   

Sensitivity patterns to house dust mites and forage mites in atopic dogs: 150 cases     

Bensignor E  Carlotti DN 《Veterinary dermatology》2002,13(1):37-42

This study investigated intradermal test reactions to extracts of six species of mites in 150 dogs with atopic dermatitis. At least one positive reaction was seen in 120 animals (80%). Dermatophagoides farinae attracted the highest number of positive reactions (108 dogs, 90% of dogs and 72% of atopic dogs showing positive reactions). Positive reactions to other mites were not uncommon, with many dogs testing positive for Dermatophagoides pteronyssinus (32% of dogs tested), Acarus siro (35%), Tyrophagus putrescentiae (30%), Glycyphagus domesticus (27%) and Lepidoglyphus destructor (23%). Sensitivity to D. farinae alone occurred commonly (57% of cases), but multiple sensitivities were seen frequently with the other mites. Cases of sensitivity to only one mite were also seen: D. pteronyssinus (five cases), T. putrescentiae (one case) and G. domesticus (one case). Further studies are needed to appreciate more clearly the precise role played by the different species of mite in canine atopic dermatitis.  相似文献   

速康解毒口服溶液对兔皮肤刺激性和过敏性的实验研究     

黄鑫  郝宝成  高旭东  刘建枝  王保海  梁剑平 《中国草食动物》2015,35(1)

试验旨在为评价动物疯草中毒解毒治疗药物——速康解毒口服溶液的临床前应用安全性,为临床推广应用提供实验依据.用速康解毒口服溶液、赋形剂(蒸馏水)局部涂抹大白兔破损皮肤及完整皮肤,并逐日观察症状表现,统计观察结果.用速康解毒口服溶液或赋形剂、2,4-二硝基氯苯(DNCB)对大白兔多次致敏,并于2周后观察大白兔的反应.结果表明:速康解毒口服溶液对家兔完整皮肤刺激反应的平均分值为零,属于无刺激药物,对破损皮肤也无明显刺激;在过敏性实验中,给药组5只家兔中1只有轻微的过敏现象,结合致敏反应评价的分值法和致敏反应发生率,速康解毒口服溶液对家兔皮肤过敏反应的分值为0.2(1/5),致敏率为20％(1/5),具有弱致敏性.结论:速康解毒口服溶液对家兔皮肤无明显刺激性,有轻微过敏现象,属于临床安全药物.  相似文献   

Induction of immune tolerance to caseins and whey proteins by oral intubation in mouse allergy model     

U. K. Shandilya  R. Kapila  S. Singh  D. Dahiya  S. Kapila  V. K. Kansal 《Journal of animal physiology and animal nutrition》2014,98(3):467-475

This study was designed to evaluate the effect of oral tolerance of caseins (CSN) and whey proteins (WP) in alleviating the allergic response to cow's milk proteins in Swiss albino mice raised on a milk protein–free diet. Oral tolerance was induced by feeding mice with 20 mg of CSN or WP once in a day for 4 days consecutively before immunization with respective protein by intraperitoneal (i.p.) injections (20 μg 200 per μl of PBS) using 2% of alum Al(OH)₃ as adjuvant. Three weeks later, oral tolerance induction was analysed in humoral and cellular compartments of CSN‐ and WP‐fed versus saline‐fed control mice groups by measuring seric and intestinal antibody responses, mRNA abundance in splenic tissue and cytokine secretion patterns. The specific serum immunoglobulin‐E (IgE) levels were significantly suppressed (p < 0.05), while sIgA was enhanced in these groups when compared with their respective saline‐fed mice. Moreover, the mRNA levels of interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) in both CSN‐ and WP‐tolerized mice were found to be significantly decreased, while the abundance of interleukin‐10 (IL‐10) and transforming growth factor‐β (TGF‐β) was increased significantly, as compared to respective control groups. Finally, cytokine profiles indicated a reciprocal decrease in IL‐4 and IFN‐γ versus an increase in IL‐10 secretions in supernatants of cultured splenocytes of tolerized mice. Taken together, these results clearly showed that oral administration of cows' milk caseins and whey proteins can induce significant hyposensitization in mice, with the participation of suppressor cytokines.  相似文献   

不同虾类的过敏原及其过敏原性   总被引：15，自引：4，他引：15  

李振兴  林洪  李明华  曹立民 《水产学报》2006,30(2):281-284

近年来食品安全问题受到越来越多人的重视，食物过敏这一食源性疾病已引起广大消费者、生产者和研究者的普遍关注。根据流行病学调查，在欧洲约有2％～2．5％的人受到食物过敏疾病的困扰，婴幼儿的发病率更高，达到4％～6％，儿童约为2％～3％，成年人食物过敏者比例相对较低，但仍有1％～2％。在联合国粮农组织提出的八大类容易引起过敏的食物中，虾蟹等甲壳类动物及其制品是其中重要的一类。我国虽然还没有类似的调查，但有关食用虾蟹等引起过敏的报道屡见不鲜。  相似文献   

Binding of canine anaphylactic antibodies on human basophils: application to canine allergy diagnosis     

JEAN SAINTE-LAUDY  CHRISTINE PROST 《Veterinary dermatology》1996,7(4):185-191

Abstract Using the technique of human basophil passive sensitization, as employed for human allergy díagnosis, we checked the ability of canine anaphylactic antibodies to sensitize human basophils. Therefore, by sensitizing human basophils with sera taken from dogs allergic to house dust mite, we demonstrated basophil activation as measured by alcian blue staining. Basophil activation was inhibited by heating dog sera at 56 °C for 6 h and by a human myeloma IgE. Basophil activation was also shown by histamine and leukotriene (LTC4) release. These results indicate canine anaphylactic antibodies bind to human basophil IgE receptors and also that they are IgE. The three methods described here for measuring basophil activation may lead to díagnostic methods applicable to canine allergy díagnosis. Resumen Mediante el método de la sensibilización pasiva de basófilos humanos como se utiliza para el díagnóstico de la alergia humana, evaluamos la capacidad de los anticuerpos anafilácticos caninos de sensibilizar basófilos humanos. Asi, sensibilizando basófilos humanos con suero extraido de perros con alergia al ácaro del polvo, demostramos la activación de basófilos medíante la tinción de Azul de Alcián. Se inhibió calentando suero canino a 56 °C durante 6 h. y por IgE de mieloma humano. La activación de los basófilos se mostró también por la liberación de histamina y leucotrieno (LTC4). Estos resultados indican que los anticuerpos caninos anafilácticos se unen a los receptores de IgE en basófilos humanos y también que son IgE. Los tres métodos descritos aqui para medir la activación de basófilos pueden llevar a métodos de díagnóstico aplicables al díagnóstico de la alergia canina. [Sainte-Laudy, J., Prost, C. Binding of canine anaphylactic antibodies on human basophils: application to canine allergy díagnosis (Union de anticuerpos anafilácticos caninos a basófilos humanos: aplicacion al díagnóstico de alergia canina). Veterinary Dermatology 1996; 7 : 185–91.] Résumé Utilisant une technique de sensibilisation passive de basophiles humains, employee pour le díagnostic allergologique chez l'homme, nous avons testé la capacité des anticorps anaphylactiques canins à sensibiliser des basophiles humains. Ainsi, par sensibilisation de basophiles humains avec des sérums provenant de chiens allergiques aux acariens de la poussière de maison, nous avons démontré l'activation des basophiles mesurée par coloration au bleu alcian. Celle-ci est inhibée par des sérums canins chauffés à 56 °C pendant 6 heures et par un myélome IgE humain. L'activation des basophiles a été aussi démontrée par libération d'histamine et de leucotriénes (LTC4). Ces résultats prouvent la présence d'anticorps anaphylactiques canins fixés à des récepteurs IgE de basophiles humains et que ceux-ci sont des IgE. Les trois méthodes décrites ici pour mesurer l'activation des basophiles peuvent être utilisées pour le díagnostic allergologique chez le chien. [Sainte-Laudy, J., Prost, C. Binding of canine anaphylactic antibodies on human basophils: application to canine allergy díagnosis (Fixation d'anticorps anaphylactiques canins sur des basophiles humains). Veterinary Dermatology 1996; 7 : 185–91.] Zusammenfassung Mit der Technik der passiven Basophilensensibilisierung beim Menschen, wie man sie für die Allergiedíagnose beim Menschen anwendet, untersuchten wir die Möglichkeit, menschliche Basophile durch kanine anaphylaktische Antikörper zu sensibilisieren. Dazu wurden humane Basophile mit Sera von Hunden sensibilisiert, die allergisch auf Hausstaubmilben reagierten. Dabei demonstrierten wir eine Basophilenaktivierung, die durch Elsässerblau-Färbung gemessen werden konnte. Der Vorgang wurde verhindert durch Erhitzen der Hundesera auf 56 °C für 6 Stunden und durch humanes Myelom-IgE. Basophilenaktivierung wurde auch durch Histamin-und Leukotrien(LTC4)-Ausschüttung gezeigt. Diese Ergebnisse zeigen, daß kanine anaphylaktische Antikörper sich an humane basophile IgE-Rezeptoren binden und auch IgEs darstellen. Die drei hier beschriebenen Methoden zur Messung der Basophilenaktivierung können zu einer díagnostischen Methode führen, die für die Diagnostik kaniner Allergie anwendbar ist. [Sainte-Laudy, J., Prost, C. Binding of canine anaphylactic antibodies on human basophils: application to canine allergy díagnosis (Die Bindung von anaphylaktischen Antikörpern des Hundes an Basophile Zellen des Menschen: Anwendung für die Allergiedíagnose beim Hund). Veterinary Dermatology 1996; 7 : 185–91.]  相似文献   

藏草乌水煎提取液体外杀灭羊虱蝇药效及皮肤毒性试验研究     

宋向东  胡永浩  班旦  梁剑平  胡毓瑶  吴金措姆  四郎玉珍  袁振杰  格桑卓嘎  拉巴次旦  郝宝成 《中国畜牧兽医》2018,45(5):1408-1416

试验旨在研究藏草乌的水煎提取液在体外杀灭羊虱蝇的药效作用及其皮肤毒性、刺激性、过敏性试验,为藏草乌的开发利用和临床应用提供理论依据。将1%、2%、4%、8%、10%藏草乌水煎提取液分别滴于衬有吸水纸和羊虱蝇的平皿中,在1、2、4、8 h时分别观察其对羊虱蝇的杀灭情况。取相同剂量1%、2%藏草乌水煎提取液均匀涂于大白兔的完整皮肤和破损皮肤,进行皮肤毒性、刺激性和过敏性试验。结果显示,给药1 h后各浓度的藏草乌水煎提取液及阳性对照组均出现了不同程度的羊虱蝇杀灭效果,4 h后阳性对照组杀灭率为100%,8 h时,1%、2%藏草乌水煎提取液杀灭率达到70%,而2%藏草乌水煎提取液较1%药物组杀灭羊虱蝇效果好;皮肤毒性试验显示,大白兔皮肤无红斑、水肿,体重变化正常;皮肤刺激性试验结果显示,藏草乌提取液对大白兔皮肤无刺激性;皮肤致敏反应结果表明,藏草乌提取液对大白兔不具有致敏性。综上所述,藏草乌水煎提取液具有良好的体外杀灭羊虱蝇作用,且皮肤用药安全。  相似文献   

Serum immunoglobulin E concentrations in West Highland White Terrier puppies do not predict development of atopic dermatitis     

DeBoer  & Hill 《Veterinary dermatology》1999,10(4):275-281

Sera from 154 West Highland White Terrier puppies between 6 and 12 weeks of age were assayed for total IgE using a sandwich ELISA method. Development of clinical signs of atopic dermatitis in these dogs was monitored by use of an annual owner questionnaire, until the dog reached 3 years of age. Of 114 evaluated dogs, skin disease severe enough to warrant veterinary examination was reported in 52 (46%) during the three study years. A diagnosis of atopic dermatitis was made by the attending veterinarian in 28 dogs (25%). Certain litters had an especially high prevalence of apparently atopic dogs, consistent with the genetic predisposition towards atopy in this breed, but clear evidence of consistent heritability was not present. The median IgE concentration in 154 puppies at 6–12 weeks of age was 0.9 units mL⁻¹, with a skewed distribution. Significant ( P < 0.01) variation in serum IgE concentrations was observed between litters, with median serum IgE concentrations for a litter ranging from 0 to 27.7 units mL⁻¹. The median serum IgE concentration in puppies that later developed clinical signs of atopic dermatitis was not significantly different from that of puppies that remained healthy. There were no apparent correlations or significant differences found between serum IgE concentration as a puppy, parental history of skin disease, and subsequent emergence of clinical signs of atopic dermatitis. We conclude that early total serum IgE determinations seem to have little usefulness in predicting the later onset of atopic dermatitis in this breed.  相似文献   

Delayed hypersensitivity reactions to sulphonamides: syndromes,pathogenesis and management     

L.A. Trepanier 《Veterinary dermatology》1999,10(3):241-248

Sulphonamide antibiotics are a commonly used, and important, group of antibiotics in veterinary dermatology. However, many of the commonly used sulphonamides such as sulphamethoxazole, sulpha-diazine, or sulphasalazine are associated with idiosyncratic drug reactions in dogs. This paper will review the clinical syndromes, pathogenesis of sulphonamide hypersensitivity reactions and current methods for predicting these occurrences.  相似文献   

Chemical Constituents of the Deep-Sea-Derived Penicillium solitum     

Zhi-Hui He  Jia Wu  Lin Xu  Man-Yi Hu  Ming-Ming Xie  You-Jia Hao  Shu-Jin Li  Zong-Ze Shao  Xian-Wen Yang 《Marine drugs》2021,19(10)

A systematic chemical investigation of the deep-sea-derived fungus Penicillium solitum MCCC 3A00215 resulted in the isolation of one novel polyketide (1), two new alkaloids (2 and 3), and 22 known (4–25) compounds. The structures of the new compounds were established mainly on the basis of exhaustive analysis of 1D and 2D NMR data. Viridicatol (13) displayed moderate anti-tumor activities against PANC-1, Hela, and A549 cells with IC₅₀ values of around 20 μM. Moreover, 13 displayed potent in vitro anti-food allergic activity with an IC₅₀ value of 13 μM, compared to that of 92 μM for the positive control, loratadine, while indole-3-acetic acid methyl ester (9) and penicopeptide A (10) showed moderate effects (IC₅₀ = 50 and 58 μM, respectively).  相似文献