寄生虫的抗原变异   总被引：2，自引：1，他引：1  

沈杰  刘一平  李东风 《中国兽医寄生虫病》2001,9(2):44-47

文章对寄生虫的抗原变异情况和消除抗原变异对免疫预防影响的主要方面进行了评述，许多种类寄生虫绝大部分表面怕变异频繁，从而逃避了宿主动物的免疫作用，变异最频繁的是各种寄生于人和哺乳动物的锥虫，其次是寄生于人和灵长类动物的几种疟原虫，再其次是寄生于哺乳动物和人的巴贝斯虫，还有几种属鞭毛虫纲、孢子虫纲和蠕虫的寄生虫，一般认为导致抗原变异的作用机制是表达抗原的基因发生了改变，正在表达的基因停止表达，而同时另一个基因却活化了，对于某些寄生虫抗原变异的规律虽已有揭示，但尚未揭示清全部主要规律，另一些种类寄生虫的抗原变异规律尚不清楚，基因活化的调控亦不甚清楚。抗原变异逃避了宿主动物的免疫，人们已提出了多种克服了的方法与设想，有些已在实验试验中出现端倪，但尚未形成临床上实用的技术，文章对它们也进行了讨论。  相似文献   

甲基对硫磷人工抗原的合成与鉴定   总被引：23，自引：1，他引：23       下载免费PDF全文

贾明宏  钱传范  韩丽君  李成文  张冰 《农药学学报》2003,5(2):22-32

根据甲基对硫磷的结构特征,分别从甲基对硫磷的硝基一端或磷酸酯基甲氧基一端引入一定长度的间隔臂和活性基团,设计并合成了4种结构的半抗原;针对各种半抗原所带活性基团的性质,通过多种方法制备了与不同的载体蛋白偶联的人工抗原。进行动物免疫后,得到了针对甲基对硫磷的高效价抗体。  相似文献   

大豆抗原蛋白对幼龄动物致敏作用的研究进展   总被引：1，自引：0，他引：1  

刘明美  赵国琦 《中国饲料》2011,(14)

大豆抗原蛋白对幼龄动物的致敏作用及不易灭活性使其成为大豆及其制品在动物养殖中有效利用的主要瓶颈。本文从主要抗原蛋白的特性、致敏机制、对幼龄动物的影响、降低抗原蛋白的方法及有待进一步探索问题等方面进行了阐述,旨在为高效利用大豆及其制品,以及进一步深入研究提供参考。  相似文献   

Detection of challenge virus in fetal tissues by nested PCR as a test of the potency of a porcine parvovirus vaccine   总被引：1，自引：0，他引：1  

Belák  S.  Rivera  E.  Ballagi-Pordány  A.  Hanzhong  W.  Widén  F.  Soós  T. 《Veterinary research communications》1998,22(2):139-146

To estimate the potency of a porcine parvovirus (PPV) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with PPV and the distribution of the virus was studied in the tissues of their 51 fetuses. Virus detection was attempted using haemagglutination (HA) and immunofluorescence (IF) assays, as well as by standard (single) and nested polymerase chain reactions (PCR). None of the detection methods yielded positive results when used to test for the presence of virus in suspensions of organs from the fetuses from the vaccinated gilts. However, the virus was detected in the fetuses from non-vaccinated gilts as follows: HA was positive in 14 cases out of 23 (60.8%), IF in 16/23 (69.5%), standard PCR in 12/20 (60%), and the nested PCR in 19/23 (82.6%). Although the correlation among the results of various methods of virus detection was rather close (r<0.83), the sensitivity of the nested PCR was the highest, both when testing dilutions of PPV and when analysing the fetal organs. The nested PCR therefore provides a reliable approach for studies of virus distribution in fetal organs, with special reference to potency tests on vaccines.  相似文献   

Corynebacterium pyrogenes; a biochemical and serological study     

G H Sorensen 《Acta veterinaria Scandinavica》1974,15(4):544-554

The study comprises 136 strains of Corynebacterium pyogenes originating from cattle (105), swine (20), sheep (1), and insects (10). For comparison 2 strains of human origin and 1 strain of Gorynebacterium hemolyticum were examined.One of the bovine strains was atypical, being gelatinase-negative, otherwise the strains of Cb. pyogenes were found to be biochemically identical apart from minor deviations in fermentation patterns (Table 1). Neither were antigenic differences demonstrated (gel diffusion analyses, Figs. 1 and 2).Both of the human strains agreed biochemically with Cb. pyogenes (Table 1). By gel diffusion cross analyses one of them was found to be identical with Cb. pyogenes, the other not, though anti-genically related to it (Fig. 2).Gb. hemolyticum deviated biochemically as well as serologically from Gb. pyogenes, but the 2 organisms shared antigenic determinants (Fig. 3).  相似文献   

鸡毒支原体灭活疫苗效力检验气囊注射攻毒方法的研究     

魏津  刘博  马欣  王甲  赵浩然  刘元杰  冯妍  刘业兵  罗玉峰 《中国兽药杂志》2022,56(10):12-17

鸡毒支原体灭活疫苗效力检验采用的喷雾攻毒方法所需菌液量大,结果易受喷雾时间和器械设备影响。为了探索一种简便、易行且结果稳定的新方法,本研究冻干了一批攻毒用菌株鸡毒支原体R株,对其性状、纯粹、真空度、剩余水分、培养特性、特异性、活菌计数和均一性等进行检定,结果全部符合规定。将其稀释成不同活菌数,采用气囊注射方式攻毒,观察试验结果并与《中国兽药典(三部)》中的喷雾方法进行比较。结果表明,建立的气囊注射攻毒法稳定、准确,通过5批次疫苗效力检验试验比较,与喷雾方法结果无显著差异,可为该类疫苗攻毒方法优化提供新思路。  相似文献   

伊氏锥虫在小鼠体内抗原变异规律及免疫保护试验     

王权  沈杰  周勇志  周金林 《中国兽医学报》2003,23(2):160-163

为了探索伊氏锥虫抗原的变异规律及其用于免疫预防的可能性，首先对伊氏锥虫安微株单虫克隆2个早期变异体ShTatl.1、ShTat1.2采用蛋白酶抑制剂TLCK处理，分离纯化这2种变异体的VSG，用ShTat1.1 VSG免疫KM小鼠，ShTat1.1锥虫攻击免疫鼠后6d分离锥虫，经间接免疫荧光试验（IFT）和班点酶标记试验（Dot-EIA)鉴定为ShTat1.2，说明同一克隆锥虫感染兔，小鼠第1次发生抗原变异后产生的变异体相同。依据这一规律，设计了免疫预防保护试验，试验小鼠分3组：一组为未免疫对照组，一组为ShTat1.1VSG单一抗原免疫组，另一组为ShTat1.1 VSG,ShTat1.2 VSG混合免疫抗原免疫组，各组均以ShTat1.1锥虫攻击，结果ShTat1.1 VSG ShTat1.2VSG免疫组小鼠全部获得保护，单用ShTat1.1 VSG免疫组小鼠和未免疫小鼠血中全部出虫、死亡，前者比后者出虫时间、存活时间延长。提示运用伊氏锥虫抗原变异这一规律设计的这种复合抗原，能激发宿主克服虫体抗原变异对免疫保护的干扰。  相似文献   

猪细小病毒VP2蛋白主要抗原域在毕赤酵母中的表达及鉴定   总被引：1，自引：0，他引：1  

李斐  郑其升  李学仁  魏凡华  于春梅  李鹏  陈溥言 《农业生物技术学报》2006,14(6):988-989

猪细小病毒(Porcine parvovirus,PPV)是一自主型细小病毒(Autonomous parvovirus),它是引起母猪繁殖障碍的主要因素之一。目前国内外的诊断方法多以全病毒为抗原,存在很大的局限性,而重组蛋白无感染性,易于生产和纯化。因此用重组蛋白来检测猪细小病中抗体水平对于猪细小病的防  相似文献   

Genomic, protein and antigenic variability of mycoplasma bovis     

F. Poumarat  M. Solsona  M. Boldini 《Veterinary microbiology》1994,40(3-4):305-321

Restriction endonuclease analysis (REA) with three enzymes SmaI, PstI, BamHI- was used to identify 13 different genomic groups among 37 Mycoplasma bovis strains. One genomic group was comprised of 14 strains. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) patterns for one strain chosen from each genomic group and an international reference strain PG45 were all similar. Antigenic variability in M. bovis species was investigated by immunoblotting, using serum from a calf that had been naturally infected with M. bovis and three M. bovis-specific monoclonal antibodies — mAbs N₂, I₂ and 5D7. Twenty M. Bovis field strains were tested, comprising one from each genomic group, six from the same genomic group and the reference strain. Antigenic profiles obtained with calf serum differed markedly one from the other, the heterogeneity being equally great among the strains belonging to the same genomic group as those coming from different groups. A stable antigen common to 164 out of 168 strains was detected by mAb N₂, whilst with mAbs I₂ and 5D7, two different membrane antigenic systems were demonstrated that were strikingly variable. These variations in expression occurred not only from one strain to another, but also within the same lineage of clones from a single cell.  相似文献   

猪传染性胃肠炎病毒NSP8蛋白单克隆抗体的制备及抗原表位的初步鉴定     

董慧  张鑫  陈建飞  时洪艳  石达  常铁城  谷凤丽  徐天  王智琴  刘明春  冯力 《中国畜牧兽医》2015,42(2):358-364

为鉴定猪传染性胃肠炎病毒(porcine transmissible gastroenteritis virus,TGEV)NSP8蛋白的抗原表位,本研究对GST-NSP8重组蛋白进行了原核表达,并用纯化后的重组蛋白免疫6周龄BALB/c小鼠,取免疫小鼠脾脏,采用常规杂交瘤细胞融合方法,经3次亚克隆后制备了1株稳定分泌抗NSP8蛋白的单克隆抗体杂交瘤细胞株。分泌的单克隆抗体亚类鉴定其重链为IgG1型,轻链为κ链;杂交瘤细胞培养上清的效价为1∶3 200。Western blotting试验结果表明该单克隆抗体能识别原核及真核表达的NSP8重组蛋白。利用截短表达的方法对NSP8蛋白进行抗原表位的鉴定,初步确定了单克隆抗体针对的抗原表位序列为31SPQILKQLTKAFNIAKSDFEREASV55。本研究制备的单克隆抗体及对抗原表位的鉴定,为TGEV NSP8蛋白相关功能的研究奠定了基础。  相似文献