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排序方式: 共有205条查询结果,搜索用时 15 毫秒
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Thomas R. De Ridder Justine E. Campbell Cheryl Burke‐Schwarz David Clegg Emily L. Elliot Samuel Geller Wendy Kozak Stephen T. Pittenger Jennifer B. Pruitt Jocelyn Riehl Julie White Melissa L. Wiest Chad M. Johannes John Morton Pamela D. Jones Peter F. Schmidt Victoria Gordon Paul Reddell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):415-429
3.
Niora J. Fabian Michael Y. Esmail Lauren Richey Sureshkumar Muthupalani Jennifer L. Haupt Joanna Joy Sebastian E. Carrasco 《Journal of veterinary diagnostic investigation》2021,33(3):582
Cutaneous leiomyosarcomas are malignant mesenchymal tumors of smooth muscle origin and are reported occasionally in avian species. A 14-y-old male laboratory White Carneau pigeon (Columba livia) was presented for surgical excision of a cervical soft tissue mass. Ultrasonography with color flow Doppler imaging revealed multiple cavitations of mixed echogenicity within the mass and vascularization. Histologically, the dermis and subcutis were expanded by a densely cellular multinodular mass comprised of fusiform cells forming haphazardly arranged broad streams and short interwoven bundles, often surrounding blood vessels and variably sized cavitations. Neoplastic cells were strongly immunopositive for desmin and α–smooth muscle actin, and negative for pancytokeratin, S100, and von Willebrand factor. Based on histopathology and IHC findings, the cutaneous mass was diagnosed as leiomyosarcoma (LMS). The pigeon died 312 d post-operatively. Postmortem examination revealed masses infiltrating the left and right pulmonary airways and one hepatic nodule, but no regrowth at the surgical site. Histologic and IHC evaluation of the pulmonary and hepatic masses were consistent with LMS, representing metastatic foci from the primary cutaneous LMS. Our case highlights the malignant behavior and histomorphologic features of cutaneous LMS in an avian species. 相似文献
4.
Nguyen Vu SON James Kenn CHAMBERS Makoto NAKATA Yasutsugu MIWA Hiroyuki NAKAYAMA Kazuyuki UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):208
This report described the histopathological and immunohistochemical features of cutaneous mast cell tumor (MCT) in six hedgehogs. The hedgehogs presented single cutaneous mass with ulcer and crusting. Histologically, the neoplastic lesions were characterized by the proliferation of well-differentiated mast cells (3 cases), and atypical mast cells (3 cases) with one atypical histiocytic morphology. Immunohistochemically, tumor cells were positive for KIT and mast cell tryptase, and were negative for Iba-1. In well-differentiated MCT, all patients were clinically improved and survived more than 365 days after surgical excision, whereas an atypical histiocytic MCT showed aggressive behavior with re-recurrence, and the animal died 115 days after surgery. These findings suggest that, compatible with other animals, well-differentiated MCT has a better prognosis in hedgehogs. 相似文献
5.
Antibacterial chemicals in the mucus of fish such as lysozyme, lectins, peptides and proteases provide an efficient first line of defence against pathogens. This study shows that there are at least three antibacterial proteins in plaice skin mucus in addition to lysozyme. One of these proteins is responsible for approximately 74% of the antibacterial activity and is a 630 kDa protease complex designated KilC (bacterial killing metalloprotease C). Purified KilC kills the bacteria Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa efficiently. The protease activity of KilC is dependent upon the divalent cation Mg(2+) and shows pH dual optima of 5.0 and 8.0. The enzyme has a temperature optimum of 25 degrees C and is made up of at least five different sized peptides. Studies with protease inhibitors show that the catalytic site of KilC may be cysteine- or serine protease-like. KilC may kill bacterial cells by acting directly upon the bacteria or by producing low molecular weight bioactive compounds such as peptides. 相似文献
6.
Flavobacterium columnare is the causative agent of columnaris disease. The presence of lesions on the gills, skin and fins of diseased fish suggests that F. columnare is able to utilize fish skin mucus as a substrate for growth and that exposure to this material would alter the expression of genes involved in the colonization of the outer surfaces of the fish. Growth, biofilm formation, extracellular protease production and changes in protein expression of F. columnare strain C#2 cultured in media supplemented with juvenile Atlantic salmon skin mucus were compared with the same media without mucus. C#2 was able to grow by using mucus as the sole nutrient source. Growth in mucus-containing media induced cells to grow as a biofilm and extracellular protease activity increased in mucus-containing cultures. SDS-PAGE protein profiles showed that expression of six extracellular proteins increased in mucus-containing media. These results demonstrate that salmon surface mucus promotes the growth of F. columnare and that exposure to mucus alters the growth characteristics of this bacterium with regard to protease production and biofilm formation. Further characterization of mucus-induced physiological changes will increase our understanding of the basis of virulence of this economically important fish pathogen. 相似文献
7.
Eui Jeong Han Hyun-Soo Kim Kalu Kapuge Asanka Sanjeewa Kyungsook Jung Youngheun Jee You-Jin Jeon Ilekuttige Priyan Shanura Fernando Ginnae Ahn 《Marine drugs》2020,18(12)
Sargassum horneri (S. horneri), an edible brown alga, has been proposed as a functional food with an improvement effect on abnormal skin immune responses. The present study investigates the anti-allergic effect of an ethanol extract from S. horneri (SHE) on immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated activation in bone marrow-derived cultured-mast cells (BMCMCs) and passive cutaneous anaphylaxis (PCA) reaction in mice. SHE markedly and dose-dependently suppressed the degranulation of BMCMCs by reducing the β-hexosaminidase and histamine release without cytotoxicity. In addition, SHE significantly decreased the FcεRI expression on the surface of BMCMCs and its IgE binding. Moreover, SHE reduced the mRNA expression and the production of allergic cytokines; interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-10, IL-13; interferon (IFN)-γ and/or tumor necrosis factor (TNF)-α; and a chemokine, thymus and activation-regulated chemokine (TARC), by suppressing the activation of Src-family kinases and nuclear factor (NF)-κB signaling. In further study, the application of SHE reduced the PCA reaction in an IgE/BSA-induced type I allergic mice model. Taken together, we suggest that SHE has an anti-allergic effect in type I allergic responses. 相似文献
8.
母牛发情周期和妊娠早期宫颈-阴道粘液电导率变化规律的研究 总被引:3,自引:0,他引:3
本文应用自行设计安装的MFR-1型母牛发情鉴定及早期妊娠诊断仪,将特制探测电极插入母牛阴道穹窿部,测定了94头母牛宫颈-阴道粘液的电导率(CVMEC)。结果表明,母牛在发情周期和妊娠早期CVMEC呈规律性变化。发情周期正常的母牛,CVMEC在间情期处于较低水平(5.64mS/cm),前情期开始上升(8.93mS/cm),发情期达峰值(13.40mS/cm),后情期开始下降(9.84mS/cm),最后降至间情期水平。输精后,未妊与妊娠母牛CVMEC的变化规律不同。在发情周期的各时期,未妊母牛高于妊娠母牛;未妊母牛在输精后21天左右返情,CVMEC再度出现峰值;妊娠早期母牛则保持与间情期相近的较低水平(5.08mS/cm)。笔者认为,母牛CVMEC的规律性变化的生理学基础是体内激素水平和宫颈-阴道粘液的周期性变化,据此进行发情鉴定和早期妊娠诊断是可行的。与目前使用的方法相比,CVMEC法更具有实用价值。 相似文献
9.
环丙沙星是高效广谱抗菌新药,将其同透皮吸收促进剂(Azone)按药剂学方法制成盐酸环丙沙星搽剂,稳定性良好.将药液涂搽于仔猪体表,用以防治哺乳仔猪大肠杆菌病,获得满意效果. 相似文献
10.
[目的]从遵义小钝吻鮠(Leioxassis microcrassirostris Zunyiensis Xiao sp.nov.)体表黏液中获取高质量的抗菌肽,为提高其先天免疫能力及病害防治效果奠定基础.[方法]采用物理方法收集遵义小钝吻鮠的体表黏液,以5%乙酸进行浸提,采用SephadexG-50和SephadexG-25两步凝胶层析技术对抗菌粗提物进行分离纯化,结合Tricine-SDS-PAGE电泳法进行分离鉴定,并运用牛津杯法和挖孔法进行抑菌试验.[结果]遵义小钝吻鮠体表黏液经乙酸法浸提处理并冷冻干燥,其粗提物呈白色粉末,稀释液蛋白质含量为1755 μg/mL;经SephadexG-50凝胶层析分离,其纯化产物抑菌活性峰主要在峰Ⅱ和峰Ⅲ,抑菌圈直径11.66 mm;再经SephadexG-25凝胶层析分离纯化后出现单一活性峰,其产物为分子量小于4.1kD的抗菌肽,抑菌圈直径12.85mm.[结论]遵义小钝吻鮠无需诱导刺激,可直接采用物理方法收集其体表黏液,以5%乙酸浸提后经SephadexG-50和SephadexG-25两步凝胶层析便可分离出纯度较高的抗菌肽. 相似文献