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应用试管二倍稀释法测定了氟砜霉素、氯霉素、TMP和OMP对15株畜禽常见病原菌的最小柳菌浓度(MIC)。结果表明,氟砜霉素的体外抗菌活性强,优于或等于氯霉素;TMP和OMP的体外抗菌活性偏低,但OMP优于TMP。氟砜霉素和氯霉素与TMP、OMP对14株细菌的联合药敏试验经肉汤稀释棋盘法测定,结果表明,氟砜霉素和氯霉素分别与TMP、OMP体外联合,抑菌效果相同,呈协同作用的均占50%与28.6%,即TMP或OMP对氟砜霉素和氯霉素的增效作用相等,但TMP优于OMP。在14株细菌对氟砜霉素和氯霉素分别与TMP、OMP的总共56组体外联合试验中,呈协同作用的占39.3%,主要为大肠杆菌、多杀性巴氏杆菌、沙门氏菌及金黄色葡萄球菌;无关作用的点60.7%;无拮抗现象。故临床联合用药前,仍需进行联合药敏试验,呈协同作用者才可联用。 相似文献
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为了研究拮抗细菌Enterobacter cloacae B8在野外水稻叶面上的定殖情况及拮抗作用,由自然选择和Tn5诱变从B8产生了一株抗菌素抗性突变体BX8.BX8能在含利福平达400μg/ml或卡那霉素达300μg/ml的LB培养基中生长.实验表明该抗菌素抗性的产生没有降低BX8的拮抗活性.该抗性比较稳定,在无抗菌素培养基中培养.BX8至少分裂生长60代内抗性不变. 相似文献
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2株柔嫩艾美耳球虫对5种抗球虫药的抗药性 总被引:8,自引:0,他引:8
选择130只21日龄雏鸡,研究了柔嫩艾美耳球虫(E.tenella)南京株和凤阳株对地克珠利(Diclazuril)、马杜霉毒(Maduramicin)、氯羟吡啶(Clopidol)、氯苯胍(Robenidine)和氨丙啉(Amprolium)的抗药性。试验鸡随机分成13组,其中每株球虫均设5个感染用药组,1个感染不用药组,另设1个不感染不用药组,每组10只鸡。以POAA,RLS,ROP,ACI作为指标。结果表明,2株柔嫩艾美耳球虫均对氨丙啉轻度抗药,对地克珠利敏感或轻度抗药,对氯羟吡啶中度抗药。对马杜霉和氯苯胍中度抗药或安全抗药。提示:目前在凤阳地区可合理地使用地克珠利和氨丙啉,对氯羟哟啶应慎用,对马杜霉素和氯苯胍须减少或暂停使用。 相似文献
6.
细菌耐药拮抗剂的研究 总被引:6,自引:1,他引:5
本文介绍了具有拮抗细菌耐药性作用的物质的研究进展情况,包括灭活酶抑制剂、药物渗透促进剂、外输泵抑制剂、细菌生物被膜抑制剂、抗菌药物增强剂、耐药质粒消除剂等。 相似文献
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H. Kruse M. Hofshagen S. I. Thoresen W. P. Bredal I. Vollset N. E. Søli 《Veterinary research communications》1996,20(3):205-214
In a retrospective study, 1538 strains of -haemolysin-producing Staphylococcus species isolated from dermatitis in dogs at three veterinary clinical microbiology laboratories in Norway during 1986–87 and 1993–94 were investigated for their antimicrobial susceptibility. None of the strains was resistant to cloxacillin, cephalexin or the quinolones enrofloxacin and ciprofloxacin. More than 96% of the strains were susceptible to trimethoprim-sulphonamide, bacitracin and fucidic acid. Between 67% and 89% of the strains were susceptible to erythromycin, lincosamides, tetracycline, neomycin and chloramphenicol. Only 37.9% of the strains were susceptible to penicillin. The frequency of penicillin resistance increased significantly between the first and second periods, from 46.0% to 58.6%. The frequency of resistance to lincomycin, clindamycin and erythromycin also increased significantly between the first and second periods, from 3.0%, 2.1% and 3.3% to 25.5%, 19.5% and 24.8%, respectively. A moderate increase in resistance to tetracycline was also noted, from 20.4% in the first to 27.6% in the second period. On the other hand, the frequency of resistance to trimethoprim-sulphonamide decreased significantly from 4.1% in the first to 0.9% in the second period. Many different resistance patterns were observed in each period. However, the proportion of multiresistant strains increased from 2.1% in the first to 10.2% in the second period. There was a decrease in resistance to the combination of trimethoprim-sulphonamide and penicillin from the first to the second period. Resistance to the combination of lincosamides and pencillin increased. For the combinations penicillin-tetracycline-lincosamides, pencillin-lincosamides-erythromycin, and pencillin-tetracycline-lincosamides-erythromycin, there was a striking increase in resistance between the first and the second periods.Abbreviations CVL
Central Veterinary Laboratory
- NCVM
Norwegian College of Veterinary Medicine
- NVL
Norwegian Veterinary Laboratory 相似文献
10.
Henk-jan Schoonbeek Johannes G.M. van Nistelrooy Maarten A. de Waard 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(9):1003-1011
The role of multiple ATP-binding cassette (ABC) and major facilitator superfamily (MFS) transporter genes from the plant pathogenic fungus Botrytis cinerea in protection against natural fungitoxic compounds was studied by expression analysis and phenotyping of gene-replacement mutants. The expression of 11 ABC (BcatrA–BcatrK) and three MFS genes (Bcmfs1, Bcmfs2 and Bcmfs4) was studied. All genes showed a low basal level of expression, but were differentially induced by treatment with cycloheximide and the plant defence compounds camptothecin, eugenol, psoralen, resveratrol and rishitin. The latter compounds induced expression of BcatrB at a high level. Eugenol was more toxic to BcatrB gene-replacement mutants than to the control isolates. Eugenol also caused an instantaneous increase in mycelial accumulation of the fungicide fludioxonil, a known substrate of BcatrB. However, there was no difference in virulence between the wild-type and BcatrB gene-replacement mutants on Ocimum basilicum, a plant known to contain eugenol. The results indicate that BcatrB is a transporter of lipophilic compounds, such as eugenol, but its role in virulence remains uncertain. 相似文献