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1.
Tatyana A. Kuznetsova Natalya N. Besednova Larisa M. Somova Natalya G. Plekhova 《Marine drugs》2014,12(2):886-898
An important problem of treating patients with endotoxemia is to find drugs to reduce the negative effects of endotoxin on the organism. We tested fucoidan (sulfated polysaccharide) from the brown alga Fucus evanescens as a potential drug in a mouse model of endotoxemia inducted by lipopolysaccharide (LPS). The survival time of mice injected with LPS increased under fucoidan treatment compared with the group of mice injected with LPS only. The preventive administration of fucoidan to mice with endotoxemia resulted in inhibition of increased levels of proinflammatory cytokines (TNFα and IL-6), as well as decreasing of the processes of hypercoagulability. The parenteral or per os administration of fucoidan resulted in decreasing the degree of microcirculatory disorders and secondary dystrophic-destructive changes in parenchymal organs of mice with endotoxemia. Taken together, these results demonstrate that fucoidan prevents endotoxin-induced damage in a mouse model of endotoxemia and increases the mice’s resistance to LPS. 相似文献
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van den Hoven R Duvigneau JC Hartl RT Gemeiner M 《Veterinary research communications》2006,30(8):921-928
On four occasions, four horses with heaves and four horses with small airway inflammatory diseases inhaled 0.9% saline based
aerosol mixtures with or without lipopolysaccharides (LPS). Prior to the first saline and LPS inhalation, horses were untreated,
while three and a half days prior to the third and forth inhalation horses had received 0.8 μ g/kg clenbuterol intravenously
twice daily.
The messenger RNA (mRNA) expression of tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10 and
interferon- γ (IFN- γ) was investigated by RT-PCR, all of which were expressed in the white blood cells of samples collected.
Inhalation of LPS only changed the cytokine expression profile of IL-10, IL-4 and TNF-α mRNA which were higher after challenge
with LPS. However in those horses that were treated with clenbuterol the LPS-induced IL-10 mRNA expression was shown to be
suppressed. Further changes in IL-4 and TNF-α were not significant. Thus the results of this study indicated that clenbuterol
can modulate the expression of IL-10 mRNA in peripheral white blood cells in those horses with small airway diseases that
have been exposed to LPS.
van den Hoven, R., Duvigneau, J.C., Hartl, R.T. and Gemeiner, M., 2006. Clenbuterol affects the expression of messenger RNA
for interleukin 10 in peripheral leukocytes from horses challenged intrabronchially with lipopolysaccharides. Veterinary Research
Communications, 30(8), 921–928 相似文献
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S. Gilani G. S. Howarth C. D. Tran R. Barekatain S. M. Kitessa R. E. A. Forder R. J. Hughes 《Journal of animal physiology and animal nutrition》2018,102(1):e486-e492
Fasting of up to 24 hr has been shown to increase intestinal permeability (IP) in chickens. The aim of this study was to determine whether fasting duration of 4.5 and 9 hr increased IP and whether l ‐glutamine (a non‐essential amino acid) supplementation before fasting provided some protection of barrier function as shown in other species. Ross 308 male broilers (n = 96) were fed either a control diet or the same diet supplemented with 1% glutamine from d0 to d38 post‐hatch. On d37, the birds were assigned to single‐bird metabolism cages and were fasted for either 0, 4.5, 9 or 19.5 hr. This study design was 2 × 4 factorial with two levels of glutamine and four levels of fasting. Birds in the 0‐hr fasting group had free access to feed. All birds had ad libitum access to water. To measure IP on day 38, following their respective fasting periods, birds were administered two separate oral gavages of fluorescein isothiocyanate dextran (FITC‐d) followed by lactulose, mannitol and rhamnose (LMR) sugars, 60 min apart. Whole blood was collected from the jugular vein 90 min post‐LMR sugar gavage. FITC‐d and L/M/R ratios were measured by spectrophotometry and high‐performance ionic chromatography respectively. Lipopolysaccharide (LPS) endotoxins in plasma of the birds fed the control diet were also measured using chicken‐specific LPS antibody ELISA. Serum FITC‐d and plasma L/M and L/R ratios for 4.5, 9 and 19.5 hr were significantly (p < .05) higher compared to the non‐fasting group. However, IP was not different in the glutamine‐supplemented group (p > .05) compared to the control group. LPS concentrations measured by the ELISA were below the detectable range. We conclude that fasting periods of 4.5 and 9 hr increased IP compared to non‐fasted birds and dietary glutamine supplementation did not ameliorate changes in IP. 相似文献
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J. M. Van Der Wolf J. R. C. M. Van Beckhoven E. De Boef N. J. M. Roozen 《European journal of plant pathology / European Foundation for Plant Pathology》1993,99(2):51-60
Sixteen bacterial strains, cross-reacting with antibodies againstErwinia chrysanthemi (Ech), were isolated from potato peel extracts, ditch water, and the rhizosphere of wheat, onion, sugar beet and chicory using the immunofluorescence colony-staining procedure. Based on fatty acid profiles, isolates were classified as belonging to thePseudomonas fluorescens group.These strains, together with two previously isolated cross-reactingP. fluorescens strains, crossreacted with polyclonal antibodies against Ech in immunofluorescence cell-staining, Ouchterlony double diffusion, and ELISA. Seventeen strains also reacted strongly with monoclonal antibodies against the lipopolysaccharides (LPS) of Ech in ELISA.Cell envelopes (CE) and proteinase-K-treated CE (mainly LPS) of cross-reacting bacteria were further characterized with SDS-PAGE and Western blotting. Based on CE protein and LPS patterns, the cross-reacting bacteria were classified into two groups, each existing of two subgroups. Both CE and proteinase-K-resistant antigens strongly cross-reacted on immunoblots with antisera against a wild type strain of Ech. With an antiserum against a LPS O-chain lacking mutant of Ech only protein bands but no proteinase-K-resistant antigens were detected on immunoblots. These data suggest that in all cases the highly antigenic LPS O-chain is responsible for the cross-reactions. 相似文献
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Maraike Wiebe Christiane Pfarrer Lara Górriz Martín Marion Schmicke Martina Hoedemaker Heiner Bollwein Maike Heppelmann 《Reproduction in domestic animals》2021,56(1):172-182
Metritis is an important disorder in dairy cows during the early postpartum period. Myometrial contractility is a prerequisite for uterine involution; however, very scanty literature is available about the effect of metritis on this process and endocrine responsiveness. This study was aimed to evaluate the effect of inflammation on uterine contractility in vitro, and the inflammation was induced by incubating myometrial strips with lipopolysaccharides (LPS). Myometrial samples were collected from 17 healthy Holstein Friesian cows during caesarean section. Eight longitudinal strips from each cow were incubated in organ baths with LPS concentrations of 0 (LPS0), 0.1 (LPS0.1), 1 (LPS1) and 10 µg/ml (LPS10). Spontaneous contractility and contractility induced by increasing concentrations of oxytocin (10–10 – 10–7 mol/L) were recorded during nine 30-min intervals (T1 to T9). The minimum amplitude (minA), maximum amplitude (maxA), mean amplitude (meanA) and area under the curve (AUC) were calculated for each time interval. LPS had an effect (p ≤ .05) on maxA, meanA and AUC. In T1, myometrial strips incubated with LPS0.1 and LPS1 had higher (p ≤ .05) maxA, meanA and AUC than the strips incubated with LPS0. In T9 without oxytocin, LPS0 led to higher (p ≤ .05) maxA, meanA and AUC than LPS0.1 and LPS1. In T8 and T9 with oxytocin, LPS1 had lower (p ≤ .05) maxA, meanA and AUC than the other LPS concentrations. Interestingly, the results show that LPS has a transient positive effect on myometrial contractility in vitro and that this effect is dependent on LPS concentration and duration of incubation. 相似文献
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本研究旨在筛选脂多糖(lipopolysaccharides,LPS)染毒小鼠的理想内参基因。试验以健康小鼠肝脏和LPS染毒后3、6、12、18 h的小鼠肝脏为研究对象,荧光定量RT-PCR评价YWHAZ、HPRT1、PPIA、ACTB和18S rRNA 5个内参基因的表达情况,并用geNorm软件分析其表达的稳定性。结果表明,除18S rRNA在LPS染毒时不能稳定表达外,其余4个内参基因在健康小鼠肝脏和LPS染毒小鼠肝脏中均能稳定表达,其中PPIA和YWHAZ的表达最稳定。本试验结果为荧光定量RT-PCR研究LPS与小鼠相互作用时mRNA表达水平提供了依据。 相似文献
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中华鳖MyD88部分序列克隆及其在组织中的表达差异分析 总被引：1，自引：0，他引：1
MyD88(Myeloid differentiation factor 88,MyD88)是多数TLRs(Toll like receptor,TLRs)信号转导过程中的关键接头分子。研究根据MyD88(Myeloid differentiation factor 88,MyD88)TIR结构域(Toll-like/IL-1 receptor domain)保守区设计简并引物,通过PCR扩增,成功地从中华鳖脾脏cDNA中扩增出351bp的目标序列。测序后经结构域和序列比较分析,扩增片段为中华鳖的MyD88 TIR结构域。该序列与大黄鱼、鸡等脊椎动物的MyD88的TIR结构域序列同源性和相似性分别为72.9%～86%和77.6%～83.6%。以热灭活嗜水气单胞菌刺激中华鳖后,经荧光定量PCR检测,在48h内,肝、脾和肾组织中MyD88 mRNA相对表达量均出现不同程度的增加,尤以脾脏中的增幅最为明显,为对照组的6.89倍;中华鳖心脏成纤维样细胞经20ng LPS刺激后1～8h,MyD88表达量有所提高,24h的表达量最高。该研究结果将为开展中华鳖天然免疫奠定良好基础。 相似文献
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The biological properties of Edwardsiella ictaluri RE-33 rifampicin-mutant and its parent strain EILO were analysed. RE-33 is an avirulent isolate used as a modified live vaccine against enteric septicaemia of catfish. Electrophoretic analysis of lipopolysaccharide (LPS) patterns showed high homology between both isolates. Further characterization of LPS by immunoblotting revealed the main differences in LPS composition. The RE-33 isolate lacks the high molecular weight bands of LPS (HMW-LPS). Outer membrane protein analysis also showed some immunological differences between RE-33 and the EILO parent strain. Only two fingerprinting techniques, fatty acid composition analysis and Biolog phenotypic profiles, were able to discriminate between both isolates. 相似文献