细胞因子对小尾寒羊胎盘成熟的影响     

葛利江  田文儒  崔琳  王学东  陈郁馨  范秀军 《中国兽医杂志》2004,40(1):7-10

为了研究细胞因子对小尾寒羊胎盘成熟的影响 ,本实验采用液相竞争法和平衡法 ,对小尾寒羊空怀期 (n=5 )和妊娠期 (n=13)第 85天、10 5天、12 5天、14 0天和 15 0天 (足月 )时的血清白细胞介素 - 1β(IL - 1β)、白细胞介素 - 6 (IL - 6 )和肿瘤坏死因子(TNF)的含量分别进行检测。结果表明 ,IL - 1β和 IL - 6含量在 12 5天时与对照组间差异极显著 (P<0 .0 1) ,TNF含量在各时期与对照组差异均显著 (P<0 .0 1或 P<0 .0 5 )。随着妊娠过程的进展 ,三种细胞因子含量逐渐增加 ,12 5天时达到最高值 ,分别为 0 .390± 0 .196 ng/ ml,5 79.8± 15 2 .8pg/ ml和 2 .348± 0 .396 ng/ ml。而后逐渐下降 ,到足月前略有回升 ,但仍低于 12 5天时的最高值。由此看出小尾寒羊在妊娠期间 ,细胞因子与妊娠之间有着密切的关系 ,前者对于维持妊娠起到重要作用。由于 14 0天至足月3种细胞因子基本稳定 ,这表明胎盘于 14 0天左右达到成熟 ,IL- 1β和 IL- 6与启动分娩有关 ,而 TNF可能参与小尾寒羊分娩的启动。  相似文献   

应用B-超对小尾寒羊早期胚胎和胎盘发育的研究     

李晶  田文儒 《畜牧兽医学报》2004,35(6):621-625

应用B-超监测10只健康的妊娠小尾寒羊,以研究早期胚胎和胎盘的发育过程。结果表明,早期胚体发育在妊娠第6周完成;妊娠30～46d,羊膜囊的短径与胎龄之间存在线性关系(R=0.9528,P<0.01),回归方程为Y=0.565065X 18.751970(Y:妊娠天数,X:羊膜囊宽)。胎盘发育期集中在妊娠前11周,在此期间胎盘突直径也可用于预测胎龄,二者之间的方程式为:Y=4.961429X-16.655714(Y:胎盘突直径,X:妊娠周),相关系数R=0.9882(P<0.01)。  相似文献   

The effect of maternal obesity on fatty acid transporter expression and lipid metabolism in the full‐term placenta of lean breed swine          下载免费PDF全文

L. Tian  S. S. Dong  J. Hu  J. J. Yao  P. S. Yan 《Journal of animal physiology and animal nutrition》2018,102(1):e242-e253

This study was conducted to evaluate the influence of back‐fat thickness (BF), at mating of sows, on the maternal and newborn circulating lipids, expression of placental fatty acids (FA) transporters and lipid accumulation in placenta. Full‐term placentas were obtained by vaginal delivery from BFI (9–14 mm; n = 37), BFII (15–19 mm; n = 43) and BFIII (20–27 mm; n = 38) sows according to BF at mating, and frozen placental sections were analysed for fat accumulation. Blood samples were collected from the sows of day 105 pregnancy and from cord blood at delivery. mRNA and protein expression levels were evaluated with real‐time RT‐PCR and Western blotting. Our results demonstrated that BFII females had significantly increased litter weight and placental efficiency, decreased maternal triglyceride (TG) and non‐esterified fatty acids (NEFA) levels, decreased maternal IL‐6, TNFα and leptin levels compared to BFIII females (p < .05). BFIII sows were associated with significantly decreased newborn TG levels, increased newborn glucose, IL‐6 and TNFα levels compared to BFI or BFII sows (p < .05). BFI and BFII females had significantly decreased placental TG, NEFA and cholesterol (CHOL) contents compared to BFIII females (p < .05). Moreover, decreased CD36, FATP1, FABP4, and FABP1 mRNA and protein and FATP4 protein expression, and increased LPL activity were also observed in BFIII group compared with BFII group (p < .05). PPARγ mRNA and protein and lipogenic genes such as SREBP‐1c, ACSL1, ACCα, FAS and SCD mRNA expression were downregulated or upregulated, respectively, in the placentas of BFIII sows compared to BFI or BFII sows (p < .05). Overall, this study demonstrated that there is no advantage, in terms of litter live size, litter weight and placental FA transport and metabolism, in performing the mating of sows with BF>19 mm.  相似文献   

胎盘食用的研究进展     

刘敏 《安徽农业科学》2016,(3)

近年来食用胎盘的做法广泛流行于国内外,该研究综述了国际科学界对动物食胎盘行为的原因和生物学意义的研究进展,总结了人类食胎盘在补充营养、缓解产后抑郁症、提高泌乳量等疗效上的争议以及对食用胎盘危害的推测,并对胎盘食用的研究方向进行了展望。  相似文献   

Comprehensive transcriptional profiling and functional study of lymphangiogenic growth factor in placentome of early-stage pregnant water buffalo     

Pooja A. R.  Yogesh Pandey  Meeti Punetha  Anju Kala  Hari Abdul Samad  Gyanendra Singh  Vikrant Singh Chouhan 《Reproduction in domestic animals》2023,58(1):60-72

The aim of the present study was to evaluate the expression and localization of lymphangiogenic factors (VEGF-C and VEGF-D), their receptor (VEGFR3) and lymphatic endothelial marker (LYVE1) in buffalo placenta during early pregnancy [EP], and to investigate the functional role of lymphangiogenic growth factors in placental lymphangiogenesis. The mRNA and protein expression of VEGF-C, VEGF-D, their receptor VEGFR3 and LYVE1 showed significant expression in EP1 (29–42 days) and EP2 stages (51–82 days) both in caruncle (maternal part) and cotyledon (foetal part) of the buffalo placenta. Immunoreactivity of VEGF-C, VEGF-D and LYVE1 was observed around the endometrial gland, in lymphatics and trophoblast cells, whereas VEGFR3 mainly localized in lymphatics of the caruncle and cotyledons. Cultured trophoblast cells were treated with VEGF-C/VEGF-D (50, 100 and 150 ng/ml) and combined doses of VEGF-C and VEGF-D (150 ng/ml) each for different time durations (24, 48 and 72 h). The mRNA expression of LYVE1 and PCNA was significantly (p < .001) upregulated with VEGF-C and VEGF-D and combined treatment (@150 ng/ml), as well as significantly downregulating Caspase-3 at 48 and 72 h. Thus, the present study provides evidence that lymphangiogenic factors are expressed in buffalo placental compartments and they may play a significant role in the regulation of placental function in water buffaloes.  相似文献   

Effects of testosterone on rat placental development     

Satoshi Furukawa  Naho Tsuji  Seigo Hayashi  Yusuke Kuroda  Masayuki Kimura  Chisato Hayakawa  Kazuya Takeuchi  Akihiko Sugiyama 《Journal of toxicologic pathology》2022,35(1):37

We investigated the morphological effects of testosterone on placental development in a rat model of polycystic ovarian syndrome (PCOS). Testosterone propionate (TP), which was subcutaneously administered to pregnant rats with 5 mg/animal from gestation day (GD) 14 to GD 18, induced a maternal weight reduction without mortality or clinical signs from GD 19 onwards. A decrease in fetal and placental weight, an increase in intrauterine growth retardation (IUGR) rates, and histological changes in the placenta were observed on GD 21 but not on GD15 or 17. Histopathologically, on GD 21, the trophoblast septa thickened, and the maternal sinusoids were narrowed in the labyrinth zone, resulting in a small placenta. Additionally, the placental weight, thickness, and histological morphology in the labyrinth zone on GD 21 in the TP-treated group were nearly identical to those on GD 17 in the control and TP-treated groups. Therefore, it was assumed that the testosterone-induced small placenta was induced in association with the developmental inhibition of the fetal part of the placentas from GD 17 onwards.  相似文献   

造血和神经相关标志物在人胎盘的表达     

王跃嗣  孟凡刚  姜昱竹  李建远  范光丽 《畜牧兽医学报》2006,37(4):368-373

为探讨人胎盘组织在造血中的作用以及研究造血和神经标志物在胎盘中的表达和在胚胎发育中的相关性，用免疫组织化学法对人胎盘组织和体外培养的胎盘组织中的贴壁细胞进行染色，观察其造血因子和神经标志物的表达。结果发现人7月龄胎盘组织血管内血细胞表达多种造血细胞因子，如SCF、VEGF、BMP-4和FGF-1和KDR，血管内皮细胞上也有表达；胎盘组织同时表达造血干细胞标记CD34、CD133和神经细胞标记物Nestin和MAP2等。而足月龄胎盘CD34、CD133、KDR和造血相关因子SCF、VEGF、BMP-4、FGF-1等弱表达，不表达Nestin和MAP2。两组胎盘切片都不表达GFAP和MBP。胎盘贴壁细胞（hPDACs）碱性磷酸酶、波形蛋白、CD133、Nestin和MAP2染色呈阳性，CD34、GFAP和MBP阴性表达。人胎盘组织和胎盘贴壁细胞表达多种造血相关因子和神经细胞标志物，提示胎盘具有造血功能，造血形成和神经发生之间可能存在基因表达叠加现象。  相似文献   

Biology of the prolactin family in bovine placenta. I. Bovine placental lactogen: Expression, structure and proposed roles     

Toru TAKAHASHI 《Animal Science Journal》2006,77(1):10-17

Bovine placenta produces an array of proteins that are structurally and functionally similar to pituitary prolactin. Bovine placental lactogen (bPL) is a glycoprotein hormone that has lactogenic and somatogenic properties. Purified bPL contains several kinds of isoforms that are created by alternative splicing and/or multiple glycosylation patterns. bPL can activate the prolactin (PRL) receptor‐mediated signaling pathway as well as PRL does. The bPL mRNA is transcribed in trophoblast binucleate cells, and synthesized bPL protein is stored in membrane‐bound secretory granules. The message encoding bPL is first detectable in trophoblast binucleate cells at approximately day 20 of gestation at, or shortly after, the appearance of binucleate cells in the trophoblast. Most binucleate cells are detected as expressed bPL in the placenta. Bovine PL may be the determinant in trophoblast differentiation. Although the biological activities of bPL have long been studied, the precise role of bPL is still largely unclear. This article reviews and discusses the biological roles of bPL, focusing on luteal function, fetal growth and pregnancy‐associated maternal adaptation, mammogenesis and lactogenesis, and placental angiogenesis. The precise biological function of bPL needs to be further evaluated.  相似文献   

对奶牛胎衣不下的认识和处理     

田粉莉  董德宽  邓贵平 《乳业科学与技术》2004,26(3):126-131

本文结合本场的生产实际，讨论了母牛胎盘的特点，胎衣不下的症状与诊断；着重讨论了本病的发病率及其影响因素，并将病因分析、归纳成一个示意图；最后综述了多项治疗和预防胎衣不下的措施，如灌服羊水，注射激素，用维生素A、D、E和亚硒酸钠，灌服中药，手术剥离以及电刺激等。  相似文献   

猪胎盘营养转运相关基因在不同妊娠期的表达     

伍治敏  胡光玲  敖政 《中国畜牧兽医》2022,49(8):3062-3071

【目的】探究不同妊娠时期猪胎盘的氨基酸、葡萄糖、脂肪酸转运体的表达模式。【方法】选择15头遗传背景、产仔数接近的杜洛克2~4胎经产健康母猪平均分为3组,所有母猪发情后使用相同公猪精液进行人工授精,在妊娠第40天(D40)、65天(D65)和95天(D95)通过麻醉分别取出每组母猪子宫,快速打开子宫分离出每个胎儿的胎盘组织,提取胎盘组织总RNA并反转录合成cDNA,利用合成的引物进行普通PCR扩增,用2.0%琼脂糖凝胶检测扩增产物。采用实时荧光定量PCR检测并比较3个时期胎盘中氨基酸、葡萄糖、脂肪酸转运体相关基因mRNA相对表达水平。【结果】PCR检测结果显示,氨基酸转运体相关基因(SLC7A1、SLC7A2、SLC7A3、SLC7A4、SLC7A10、SLC1A3、SLC1A5、SLC38A10、SLC36A1)、葡萄糖转运体相关基因(SLC2A1、SLC2A2、SLC2A3、SLC2A10、SLC2A12、SLC2A13)及脂肪酸转运体相关基因(FATP1、FATP2、FATP3、FATP4、FABP3、FABP5、FABP7、CD36)的片段长度均与预期相符。实时荧光定量PCR结果显示,在氨基酸转运体中,D65胎盘中SLC7A4、SLC7A10、SLC38A10基因表达水平显著高于D40胎盘(P＜0.05),而SLC7A2基因表达水平显著低于D40胎盘(P＜0.05),且D65胎盘的SLC1A3和SLC7A4基因表达水平均显著低于D95胎盘(P＜0.05);在葡萄糖转运体中,D65和D95胎盘的SLC2A3和SLC2A13基因表达水平显著高于D40胎盘(P＜0.05),D95胎盘的SLC2A1、SLC2A2和SLC2A12基因表达水平显著低于D65胎盘(P＜0.05);在脂肪酸转运体中,D65胎盘的FATP2、FATP4、FABP3、FABP5、FABP7和CD36基因表达水平显著高于D40胎盘(P＜0.05),而FATP1、FATP4和CD36基因表达水平显著低于D95胎盘(P＜0.05)。【结论】在猪妊娠过程中,胎盘中SLC7A10、SLC38A10、SLC7A4、SLC2A3、FATP1、FATP4、FABP5、CD36等基因可能是影响胎儿生长发育的重要营养转运基因。  相似文献