Expression of a recombinant crustacean hyperglycemic hormone of the kuruma prawn Penaeus japonicus in methylotrophic yeast Pichia pastoris     

TSUYOSHI  OHIRA  HIDEKAZU  KATAYAMA  KATSUMI  AIDA  HIROMICHI  NAGASAWA 《Fisheries Science》2003,69(1):95-100

ABSTRACT:   Until now, six crustacean hyperglycemic hormones (CHH) designated Pej-SGP-I, -II, -III, -V, -VI and -VII have been characterized in the kuruma prawn Penaeus japonicus . All CHH consist of 72 amino acid residues and have an amidated carboxyl (C)-terminus. In the present study, we expressed Pej-SGP-III in methylotrophic yeast Pichia pastoris in order to obtain a large quantity of recombinant CHH possessing biological activity. A cDNA encoding Pej-SGP-III that had been previously cloned was processed by polymerase chain reaction (PCR) and the resulting product was ligated into an expression vector. Pichia pastoris was transformed with this vector after which a recombinant Pej-SGP-III was expressed having an additional amino acid residue (glycine) at the C-terminus (rPej-SGP-III-Gly), a form considered to be a putative precursor of this hormone. rPej-SGP-III-Gly secreted into the culture medium was purified by reversed-phase HPLC, and amidated using a peptidylglycine alpha-amidating enzyme. The amidated rPej-SGP-III (rPej-SGP-III) showed hyperglycemic activity in in vivo bioassay almost comparable to that of the natural Pej-SGP-III. rPej-SGP-III thus obtained will be a useful tool not only for its physiological study but also for the determination of its 3-D structure.  相似文献   

Variation and distribution of virulence phenotypes of Bremia lactucae in natural populations of Lactuca serriola     

A. Lebeda†  I. Petr&#;elová 《Plant pathology》2004,53(3):316-324

During 1997–2000 the virulence variation and distribution of virulence phenotypes of Bremia lactucae (lettuce downy mildew) in natural populations of Lactuca serriola (prickly lettuce) were studied. Altogether 139 isolates of B. lactucae originating from the Czech Republic, France and Germany were examined for the presence of 27 virulence factors (v-factors) and their combinations. In the Czech population, 37 different v-phenotypes (P1–P37) of B. lactucae were found to occur on L. serriola . Most v-phenotypes were characterized by v-factors that match resistance ( Dm genes/R-factors) carried by L. serriola . A wide diversity of v-phenotypes was recorded every year, but most were rare and did not reappear in other populations of B. lactucae . The three v-phenotypes P21, P28 and P29 were most frequent and widely distributed. There was variation both between spatially isolated populations and within populations of the pathogen. Geographic differences in virulence were found for the southern parts of Moravia, where the phenotypic composition of pathogen populations was completely different from the remaining part of the area investigated. However, some populations had v-phenotypes similar to those of spatially distant populations in Moravia.  相似文献   

Phosphorus bioavailability in ash from straw and sewage sludge processed by low‐temperature biomass gasification     

《Soil Use and Management》2018,34(1):9-17

Reuse of phosphorus (P) from waste streams used for bioenergy conversion is desirable to reduce dependence on nonrenewable P resources. Two different ash materials from low‐temperature biomass gasification of wheat straw and sewage sludge, respectively, were investigated with regard to their P bioavailability. A set of pot experiments with spring barley was carried out to compare the ash P fertiliser value with mineral P fertiliser and the sewage sludge feedstock. An indirect radioactive labelling approach with ³³P was used to determine the amount of P taken up from the fertiliser materials. Depending on the application rate, straw gasification ash produced a fertiliser response comparable to mineral P. However, P uptake from the ash was generally less than uptake from equivalent amounts of mineral P, and the calculated relative effectiveness was 44% after 6 weeks of plant growth. In contrast, the P fertiliser value of Fe‐rich sewage sludge after low‐temperature gasification was practically zero. These results suggest that ash from low‐temperature gasification could be developed into alternative P fertilisers; however, as the P bioavailability depends greatly on the feedstock used, a greater emphasis on feedstock composition is required.  相似文献   

Application of body size spectra to nematode trait-index analyses     

《Soil biology & biochemistry》2015

Current trait-based indices of soil fauna for assessing soil quality rely on extensive knowledge of life history traits for hyper-diverse groups. We look at the effects of clear-cut forest harvesting and subsequent wood ash application on the free-living nematode community using trait-based indices and body size spectra. We test the hypothesis that body size is a unifying trait and that the body size spectra can be used to demonstrate changes in soil quality while overcoming taxonomic impediments. Undisturbed boreal forest floor soils were compared with clear-cut sites amended with zero, one-half calcium equivalent wood ash, equivalent calcium reintroduction, and twice calcium reintroduction. Total abundance, trait-based metrics for the Maturity Index, and two metrics of body size spectra were calculated. Nematode abundance was not a good indicator of soil disturbance, while traditional trait-based approaches (the summed Maturity Index) demonstrated predicted changes in community structure. Analysis of two types of body size spectra elucidated how the community had changed under disturbance, and revealed that smaller bodied organisms were lost under ash amended treatments compared to both clear-cut and undisturbed forest. Using the body size spectra for nematodes is a simple addition to current trait-based approaches that can supplement soil assessments for disturbance and quality without requiring taxonomic expertise.  相似文献   

Property of Protecting Reinforcing Steel Bar in Fly Ash Cement     

WU Jian-hu  PU Xin-cheng  LIU Fang  WANG Chong 《保鲜与加工》2005,(6):57-59

  相似文献   

外加剂和掺和料对砂浆性能影响的     

曾力  孙永波 《中国农村水利水电》2005,(11):75-76

当前，在水工棍凝土工程中，抹而砂浆的强度和抗渗性能越来越受到人们的重视通过对比试验，研究了减水剂、引气剂单掺、复掺及与硅粉、粉煤灰复掺，对砂浆强度和抗渗性能的影响，并从机理方面分析它们改善砂浆性能的原因结论是:减水剂、引气剂与硅粉、粉煤灰适当配比复掺，对砂浆的强度和抗渗性能的改善显著  相似文献   

加热炉管外积灰及减少积灰的措施     

钱建华 《油气储运》1996,15(1):46-47

分析了输油管道直接式加热炉炉管积灰的形成、灰垢的种类和成分。认为灰垢就是燃料油燃烧后残留下来的不可燃部分Ｎａ、Ｋ、Ｖ、Ｍｇ等金属固体盐类和其它杂质，以及碳元素在燃烧不完全的情况下残留下的可燃组分的微粒。指出燃料灰分含量的高低、燃烧器雾化质量好坏、燃烧空气温度的高低及操作人员的技术水平的高低都是影响灰垢生成量的主要因素。分析了辐射室炉管外壁和对流炉管外壁的积灰规律。指出管外积灰增加了热阻，影响传热效  相似文献   

Safety of Improved Milbond-TX When Fed in Broiler Diets Limiting in Available Phosphorus or Containing Variable Levels of Metabolizable Energy     

《The Journal of Applied Poultry Research》2007,16(3):412-419

Improved Milbond-TX (IMTX), a hydrated sodium calcium aluminosilicate, was added to broiler diets at 0, 1, or 2% in 2 experiments. In experiment 1, three concentrations of available P, 0.22, 0.32, and 0.42%, were combined with the IMTX in isocaloric and isonitrogenous corn-soybean meal-based diets and fed to male broilers for 21 d. In experiment 2, the IMTX was added to isonitrogenous broiler diets calculated to contain 2,940, 3,020, or 3,100 kcal of ME/kg and also fed for 21 d. Deficient dietary P reduced feed intake and BW at 7, 14, and 21 d of age, but IMTX had no effect even when fed at 8 times the amount recommended by the manufacturer (0.25%) to ameliorate the toxic effects of feed mycotoxins. Similarly, increasing the energy level of the diet resulted in improved BW and feed conversion, but IMTX fed at 8 times the amount recommended by the manufacturer had no detrimental effect on chick performance. There was no interaction found between IMTX and energy or available P for any performance measure. Addition of IMTX to the diet above the recommended concentration of the manufacturer also had no detrimental effect on percentage of toe ash or excreta moisture.  相似文献   

白蜡杆软化和防蛀技术研究   总被引：1，自引：0，他引：1  

钱世江  孙晓薇  于磊  金钰 《河南林业科技》2008,28(4)

白蜡(Fraxinus chinenesis)为木犀科(Oleaceae)白蜡树属(Fraxinus)的树种,落叶乔木或灌木。主要分布在西南、华中、华东、华北等地区。因白蜡杆木材具有材色浅、结构细、纹理直、软硬度适中、韧性好、可任意造型等优点,广泛用于农具、家具,特别适于弯曲部件、工艺品和文休用品的制作。自90年以来,以蜡杆、蜡条为主要原料生产高档工艺家俱的工作已经起步,但由于生产过程中缺乏合理实用的软化工艺及设备、防蛀工艺及设备,关键技术缺乏研究,不仅生产效率低,产品的内在质量无法改善,而且残次品率高,虫蛀率也高达80%以上。该研究提出了以蒸汽软化处理工艺、负压浸注防蛀工艺技术,既有效的软化了白蜡杆,同时达到了防蛀要求,产品的内在质量得到了改善。  相似文献   

贵州典型地区放牧肉山羊营养状况调查     

杨保平  ;高雅琴  ;阎奋民  ;齐志明  ;张文远  ;朱晓兵  ;赵熙贵  ;吴蔚雄  ;张德宏 《家畜生态》2008,(2):76-77

贵州典型地区比较适宜于山羊的养殖。通过对放牧肉山羊营养状况的初步调查发现,肉山羊的蛋白质营养水平偏低;在矿物质方面,存在钠的不足,铁、铜处于临界状态,钙、镁、硒、锰、锌则不缺乏。对于快速生长的幼山羊和泌乳母羊也应注意钙的补充。  相似文献