Quantitative trait loci responsible for the difference in γ-oryzanol content in brown rice between japonica-type and indica-type rice cultivars     

Tsuneo Kato  Tetsuya Matsukawa  Akira Horibata 《Plant Production Science》2017,20(4):459-466

γ-Oryzanol is a main oleophilic component in rice bran oil and has been well recognized as a good dietary supplement for human health, as well as having uses in industrial materials. japonica-type rice cultivars generally showed significantly higher contents of total γ-oryzanol in brown rice compared with indica-type cultivars, although within-group variation was significant. The objective of this study was to explore quantitative trait loci (QTLs) responsible for the difference in the γ-oryzanol content between japonica-type and indica-type rice cultivars, using recombinant inbred lines (RILs), backcross inbred lines (BILs), and corresponding chromosome segment substitution lines (CSSLs) derived from crosses between japonica-type and indica-type. Results from RILs and BILs showed that eight QTLs were detected with R² from .09 to .16. Nine candidate regions for QTL were also suggested from corresponding CSSLs. These QTLs from RILs and BILs and the candidate regions from CSSLs were not overlapped, although one QTLs was mapped near the boundaries of the respective candidate region. At four QTLs and three candidate regions, alleles or segments from japonica-type caused higher contents than those from indica-type. On the other hand, at the other four QTLs and six candidate regions, alleles or segments from indica-type caused higher contents than those from japonica-type, which is a reverse result to the parental differences. This result strongly suggested that alleles with increasing effects on γ-oryzanol content could be accumulated not only from japonica-type but also from indica-type, leading to a potential for increase in γ-oryzanol content in future breeding programs.  相似文献   

Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage     

Pingxi Wang  Hongwei Zhang  Demar lyle  Dongdong Li  Guoying Wang  Qingchun Pan  Jianhua Wang 《Plant Breeding》2019,138(3):252-258

Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC₃ generation, which accorded with the simulation result. The BPP‐based BC₃F_1:2 population could identify most quantitative trait loci (QTL) detected using the F_2:3 population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP‐based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high‐coverage introgression populations.  相似文献   

A genetic linkage map in southern‐by‐spring oat identifies multiple quantitative trait loci for adaptation and rust resistance     

Frdrick G. Sunstrum  Wubishet A. Bekele  Charlene P. Wight  Weikai Yan  Yuanhong Chen  Nicholas A. Tinker 《Plant Breeding》2019,138(1):82-94

We developed 178 recombinant inbred lines from a southern‐by‐spring oat population designated as “TxH.” These lines were genotyped to generate a high‐quality linkage map that resolved 6,902 markers into 21 linkage groups that matched closely with the latest hexaploid oat consensus map. Three major quantitative trait loci (QTLs) affecting heading date were found in locations that are consistent with known QTLs and candidate genes, and two other QTLs affecting heading date were found in novel locations. Five QTLs affecting plant height were found. Both sets of QTLs are responsible for transgressive segregation observed for these two traits. Four QTLs affecting resistance to crown rust, caused by the pathogen Puccinia coronata f. sp. avenae, were identified. Two of these QTLs are consistent with known clusters of rust resistance genes, while two may represent new locations of novel rust resistance genes. A complete set of SNP sequences suitable for generating markers for molecular selection is provided.  相似文献   

以球果量、雄花量等数量性状对油松优良无性系的再选择   总被引：1，自引：0，他引：1  

刘梅  安守芹  吕馨  王永红 《内蒙古林业科技》2001,(Z1):6-9

本研究对油松无性系种子园 2 2个无性系的球果量和雄球花量进行观测调查 ,并对数据进行处理。通过无性系内和无性系间的方差分析、聚类分析和秩序排序法对各无性系进行分类和排序。研究结果表明 :无性系间球果量和雄球花量的差异极显著 ,而无性系内差异不显著。并筛选出 1 61 #,6#,2 9#,63 #,78#5个球果数量大 ,占总数的 46% ,雄花量高 ,占总数的 5 7.6%的无性系为优良无性系  相似文献   

薄层扫描法测定人工诱生牛黄中主要成分的含量     

王芳生  赵英举  李竟成  白燕  徐礼燊  刘爱茹  张秀琴 《中国兽医学报》1989,(4)

应用岛津CS-910型薄层扫描仪测定了5份人工诱生牛黄和3份天然牛黄中胆酸(CA)、去氧胆酸(DCA)和胆红素(BR)的含量。CA和DCA用荧光扫描,λ_(ex)470nm和λ_(em)550nm;BR用可见光扫描,λ_S402nm和λ_R650nm。测定结果表明,前者中CA和DCA的含量高于后者;BR的含量则前者低于后者。  相似文献   

Quantitative site classification in the key county in the conversion of farmland to forests project     

Shidong Li  Guofang Shen  Mingpu Zhai  Junqing Li 《Frontiers of Forestry in China》2006,1(2):157-162

According to the requirements of the conversion of farmland to forests project (CFFP), we investigated the vegetation factors and environmental factors from more than 6,105 sub-compartments in Liangcheng County, Inner Mongolia by using the Matlab, analytic hierarchy process (AHP), and the hierarchical cluster method (HCM). The site conditions were classified quantitatively. The results show that CFFP at this site comprises five site-type groups and 19 site types. A quantitative site classification system method has been established in this paper. __________ Translated from Journal of Beijing Forestry University, 2005, 27(6) [译自: 北京林业大学学报, 2005, 27(6)]  相似文献   

Genetic mapping and utilization of quantitative trichome-mediated insect resistance in potato     

M. Bonierbale  R. Plaisted  S. Tanksley 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(Z2):211-214

Introgression of trichome-mediated insect resistance from the wild speciesSolanum berthaultii has become a major focus of the potato improvement program at Cornell University during the past twelve years. Several quantitative characters are involved in this resistance which is effective against a wide range of pest types. Correlative biochemical assays have been developed to assay specific components of the resistance, and the effects of the resistance on the target pests have been studied. Quantitative laboratory assays and specific measurements of insect behavior and biology have increased the precision of selection and enable the investigation of the genetic control of the resistance.We are currently using restriction fragment length polymorphisms (RFLPs) for genetic mapping of factors controlling the trichome traits fromS. berthaultii. Backcrosses to both the wild and the cultivated species parents have been evaluated for phenotypes contributing to the resistance mechanism, including trichome density, sucrose ester and polyphenol oxidase production by the trichomes, and the enzymatic browning reaction responsible for insect entrapment. Genetic maps are being developed for these progenies, using RFLP markers previously mapped in potato. Field and greenhouse trials under insect infestations are also being conducted with the mapping progeny. Our goal is to locate genes responsible for quantitative insect resistance by correlating RFLP variation at mapped loci with the trichome phenotypes and insect resistance. Genetic markers for these traits will be useful in transfer of the effective wild chromosomal segments into and among tetraploid potatoes, and for a better understanding of the resistance mechanism.  相似文献   

高效液相色谱法定量分析甲氨基阿维菌素苯甲酸盐   总被引：8，自引：1，他引：8  

毕富春  赵建平 《农药科学与管理》2003,24(3):13-14

研究用HPLC定量分析甲维盐的方法。色谱条件如下:150mm×4.6mm不锈钢柱,用Hypersil,C185μm粒径,孔径300A;流动相:甲醇+水=80+20;流速1.0mL/min;紫外检测器波长254nm。本方法在1～8μg进样范围内和峰高呈线性。相关系数值(r)为0.9997。甲维盐分析方法的回收率为99.42%。  相似文献   

Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR   总被引：4，自引：1，他引：4  

D. W. Cullen  A. K. Lees  I. K. Toth  J. M. Duncan † 《Plant pathology》2002,51(3):281-292

Colletotrichum coccodes is the causal agent of the potato blemish disease black dot. Two PCR primer sets were designed to sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a nested PCR. The genus-specific outer primers (Cc1F1/Cc2R1) were designed to regions common to Colletotrichum spp., and the species-specific nested primers (Cc1NF1/Cc2NR1) were designed to sequences unique to C . coccodes . The primer sets amplified single products of 447 bp (Cc1F1/Cc2R1) and 349 bp (Cc1NF1/Cc2NR1) with DNA extracted from 33 European and North American isolates of C. coccodes. The specificity of primers Cc1NF1/Cc2NR1 was confirmed by the absence of amplified product with DNA of other species representing the six phylogenetic groups of the genus Colletotrichum and 46 other eukaryotic and prokaryotic plant pathogenic species. A rapid procedure for the direct extraction of DNA from soil and potato tubers was used to verify the PCR assay for detecting C. coccodes in environmental samples. The limit of sensitivity of PCR for the specific detection of C. coccodes when inoculum was added to soils was 3·0 spores per g, or the equivalent of 0·06 microsclerotia per g soil, the lowest level of inoculum tested. Colletotrichum coccodes was also detected by PCR in naturally infested soil and from both potato peel and peel extract from infected and apparently healthy tubers. Specific primers and a TaqMan fluorogenic probe were designed to perform quantitative real-time (TaqMan) PCR to obtain the same levels of sensitivity for detection of C. coccodes in soil and tubers during a first-round PCR as with conventional nested PCR and gel electrophoresis. This rapid and quantitative PCR diagnostic assay allows an accurate estimation of tuber and soil contamination by C. coccodes .  相似文献   

陕甘宁老区脆弱生态环境定量评价——以榆林、延安两市为例   总被引：4，自引：0，他引：4  

杨新  延军平 《干旱区资源与环境》2002,16(4):87-90

位于黄土高原中部的陕甘宁老区生态环境极为脆弱 ,近年来由于气候、人类开发资源等自然和人为原因 ,使生态环境的脆弱程度升高。选定年降水量、年均温、蒸发量等 8个指标 ,定量评价各县 1 970 - 2 0 0 0年的脆弱度状况 ,结果表明榆林、延安两市生态环境整体脆弱 ,脆弱度存在空间差异但差异不明显 ,时间段上的波动幅度不大。陕甘宁老区脆弱生态环境具有不稳定性 ,对外界干扰较敏感。  相似文献