Survival of Leptosphaeria maculans in soil on residues of Brassica napus in South Australia     

M. R. Sosnowski  E. S. Scott  M. D. Ramsey 《Plant pathology》2006,55(2):200-206

The survival of Leptosphaeria maculans , which causes phoma stem canker (blackleg), on oilseed rape residues ( Brassica napus ) in South Australia was investigated. Using a quantitative polymerase chain reaction (PCR) assay for L. maculans DNA, the pathogen was mainly detected in the upper 5 cm of the soil profile, including residues on the soil surface. As the size of organic matter particles in the soil decreased, so did the quantity of L. maculans detected in them. To obtain representative data for a field, at least 30 subsamples needed to be collected over the 0·81 ha area studied. In a survey of 49 commercial fields in South Australia, most L. maculans was detected in fields 1 year after oilseed rape had been grown, with less detected after 2 years and negligible amounts 3 years or more after cropping. The diagnostic DNA-based assay for L. maculans reduced the time and cost of studying L. maculans survival in soil and increased the sensitivity and accuracy of results compared with estimates of propagule number of colony-forming units on a semiselective medium.  相似文献   

Effect of Completion of Vernalization on Generative Development of Winter Rape (Brassica napus L. var. oleifera) Plants     

W. Filek  F. Dubert 《Journal of Agronomy and Crop Science》1994,172(1):29-37

Germinating seeds and young plants of winter rape var. Górczañski were vernalized for 56–63 days under conditions of 9-hour day, at the temperature 2 and 5 °C and in continuous darkness at the temperature 2 °C. After vernalization the plants grew under conditions enabling to complete vernalization: in a glass-house at the temperature day/night 15/10 °C and in semi natural conditions of open vegetation hall in the period from June till August. After sub-optimal vernalization further growth of the plants at lowered temperature increased its effectiveness (completion of vernalization). Depending on the degree of the vernalization of the plants the completion of their vernalization was both obligatory, i.e. conditioning the acquisition of the ability of generative development, and facultative i.e. accelerating this development. It has been demonstrated that the population of plants of the examined variety is strongly differentiated not only with respect of vernalization requirements in the particular plants, but also what regards the effectiveness of vernalization completion. New observations have been made indicating that the mechanisms controlling the successive phases of generative development, i.e. phase of forming flower buds and the flowering phase are not identical which may be interpreted as indicating that the "flowering factor" is polymorphous.  相似文献   

Acquired resistance triggered by elicitins in tobacco and other plants   总被引：17，自引：0，他引：17  

Philippe Bonnet  Eva Bourdon  Michel Ponchet  Jean -Pierre Blein  Pierre Ricci 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(2):181-192

Elicitins are a family of proteins excreted byPhytophthora spp. They exhibit high sequence homology but large net charge differences. They induce necrosis in tobacco plants which then become resistant to the tobacco pathogenPhytophthora parasitica var.nicotianae. In stem-treated plants, resistance was not restricted to the site of elicitin application, but could be demonstrated by petiole inoculation at all levels on the stem. Resistance was already maximum after two days and lasted for at least two weeks. It was effective not only towardsP. p. var.nicotianae infection, but also against the unrelated pathogenSclerotinia sclerotiorum. In contrast to dichloroisonicotinic acid, an artificial inducer of systemic acquired resistance, which was increasingly effective with doses ranging from 0.25 to 5mole per plant, the basic elicitin cryptogein exhibited a threshold effect, inducing near total resistance and extensive leaf necrosis above 0.1 nmole per plant. Between 1 and 5 nmole, acidic elicitins (capsicein and parasiticein) protected tobacco plants with hardly any necrotic symptom. Elicitins exhibited similar effects in various tobacco cultivars andNicotiana species, although with quantitative differences, but induced neither necrosis nor protection in other SolanaceÆ (tomato, petunia and pepper). Among 24 additional species tested belonging to 18 botanical families, only some BrassicaceÆ, noticeably rape, exhibited symptoms in response to elicitins, in a cultivar-specific manner. Elicitins appear to be natural specific triggers for systemic acquired resistance and provide a tool for unraveling the mechanisms leading to its establishment.Abbreviations AR acquired resistance - HR hypersensitive response - INA 2,6-dichloroisonicotinic acid - Ppn Phytophthora parasitica var.nicotianae - SAR systemic acquired resistance  相似文献   

Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress   总被引：6，自引：0，他引：6  

E J J Momoh  W J Zhou  & B Kristiansson† 《Weed Research》2002,42(6):446-455

Summary A substantial amount of seed is left in the fields before and during harvest of oilseed rape. Although this crop exhibits little or no primary dormancy, the absence of certain environmental cues that promote germination of imbibed seeds induces secondary dormancy. The work reported investigated the extent to which environmental stress conditions, including osmotic stress, low oxygen stress and anaerobiosis, induce secondary dormancy in oilseed rape, and examined the variation in development of secondary dormancy between and within genotypes. Osmotic stress was most effective in inducing dormancy. Anaerobic treatment produced very few dormant seeds, as did an atmosphere low in oxygen and high in nitrogen. The development of secondary dormancy under osmotic stress varied considerably between and within genotypes. Dormancy ranged from almost zero to about 60% for winter genotypes and about 85% for spring types. Within genotypes, variations occurred between seed lots and years of harvest. Temperature variations affected the percentage of dormant seeds. More dormant seeds were likely to be produced with incubation under water stress at 20 °C than at 12 °C. In winter genotypes, fewer dormant seeds were produced when incubation temperature and germination test temperatures differed. Thus, incubating at 20 °C and 12 °C, followed by germination tests at 20 °C and 12 °C, respectively, produced most dormant seeds. Also, in the winter genotypes, the potential development of secondary dormancy was positively correlated with the pattern and speed of germination of untreated seeds.  相似文献   

Sporulation of Pyrenopeziza brassicae (light leaf spot) on oilseed rape (Brassica napus) leaves inoculated with ascospores or conidia at different temperatures and wetness durations   总被引：1，自引：0，他引：1  

Z. Karolewski  N. Evans  B. D. L. Fitt  A. D. Todd  A. Baierl 《Plant pathology》2002,51(5):654-665

In controlled environment experiments, sporulation of Pyrenopeziza brassicae was observed on leaves of oilseed rape inoculated with ascospores or conidia at temperatures from 8 to 20°C at all leaf wetness durations from 6 to 72 h, except after 6 h leaf wetness duration at 8°C. The shortest times from inoculation to first observed sporulation ( l ₀), for both ascospore and conidial inoculum, were 11–12 days at 16°C after 48 h wetness duration. For both ascospore and conidial inoculum (48 h wetness duration), the number of conidia produced per cm² leaf area with sporulation was seven to eight times less at 20°C than at 8, 12 or 16°C. Values of Gompertz parameters c (maximum percentage leaf area with sporulation), r (maximum rate of increase in percentage leaf area with sporulation) and l ₃₇ (days from inoculation to 37% of maximum sporulation), estimated by fitting the equation to the observed data, were linearly related to values predicted by inserting temperature and wetness duration treatment values into existing equations. The observed data were fitted better by logistic equations than by Gompertz equations (which overestimated at low temperatures). For both ascospore and conidial inoculum, the latent period derived from the logistic equation (days from inoculation to 50% of maximum sporulation, l ₅₀) of P. brassicae was generally shortest at 16°C, and increased as temperature increased to 20°C or decreased to 8°C. Minimum numbers of spores needed to produce sporulation on leaves were ≈25 ascospores per leaf and ≈700 conidia per leaf, at 16°C after 48 h leaf wetness duration.  相似文献   

Study of Resistance to Orobanche ramosa in Host (Oilseed Rape and Carrot) and Non-host (Maize) Plants     

Naïma Zehhar  Pascal Labrousse  Marie-Claire Arnaud  Christian Boulet  Driss Bouya  André Fer 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(1):75-82

Orobanche ramosa is a parasitic Angiosperm responsible for severe yield losses in several economical crops. It is a serious threat in oilseed rape in France and Morocco and is appearing in carrot crops in Morocco. In this study, several varieties of oilseed rape and carrot were screened in order to identify resistant cultivars and to characterize the resistance mechanisms involved. All the 15 oilseed rape varieties tested were susceptible. In carrot, the varieties 'Colmar à coeur rouge' and 'Nantaise demi-longue' were susceptible, whereas 'Palaiseau' and 'Buror' were resistant. In the susceptible 'Colmar à coeur rouge' carrot no defence reactions were found and the development of the parasite inhibited carrot tap root formation. In the resistant carrot varieties, the parasite germinated, became attached to the host root but became necrotic before emergence. In 'Buror' carrot, formation of a mechanical barrier was associated with the restriction to the cortex of the parasite. In maize cv. 'Vigni', a non-host of O. ramosa, thickening of xylem vessels, cell divisions in the central cylinder and formation of an encapsulation layer were observed in association with restricted development of Orobanche haustoria.  相似文献   

Effects of Rapeseed Green Manure on Soil Fertility and Bacterial Community in Dryland Wheat Field   总被引：2，自引：0，他引：2  

LI WenGuang  YANG XiaoXiao  HUANG ChunGuo  XUE NaiWen  XIA Qing  LIU XiaoLi  ZHANG XiaoQi  YANG Si  YANG ZhenPing  GAO ZhiQiang 《中国农业科学》2019,52(15):2664-2677

  相似文献   

两系杂交油菜早中熟组合精量直播栽培性状比较研究     

潘典进  罗传贵  杨玲丹  云琴  王军华  龚元春  黄春祥 《现代农业科技》2015,(2):13-14

开展优质核不育两系杂交油菜早中熟新组合比较试验,结果表明:2种熟期类型杂交组合间产量差异明显,中熟组合单位面积产量极显著高于早熟组合,对比分析产量构成因子,主要是平均角粒数多和千粒重重,2种熟期类型组合间株高和一次分枝数差异较小,油菜菌核病感病程度中熟组合相对较轻。因此,油菜生产不可一味选择和使用早熟类型品种或组合,不然将较难获得理想产量和生产效益。  相似文献   

优质油菜施硫效应研究     

杨宗飞  杨丽琼 《北京农业》2011,(3):95-97

试验表明,在土壤偏碱性,有效58.75mg/kg条件下,玉米作物施硫具有显著的增产、增值效果。施硫对提高单位面积有效穗数、平均穗籽粒数、千粒质量、叶色深度具有显著的影响,对生育时段影响较小,增产、增值效应随用量不同而异,增产9.33～26.83kg/667m~2,平均产量19.29kg/667m~2,增产率5.76%～16.59%,投产比10.17～4.32,合理用量为纯硫。2.00～4.00kg/667m~2,做底肥施用。  相似文献   

基于WSN的油菜生长环境数据采集系统     

李建辉  廖桂平  王访 《湖南农业大学学报(自然科学版)》2013,39(6):675-682

针对传统作物生长环境数据获取手段实时性差、劳动强度大以及部署微型自动气象站和商用Zigbee产品成本高、开放性较差等问题，设计并实现了一种基于WSN的油菜生长环境数据采集系统。提出了轻量级的能量感知路由协议CLFP，并给出了软硬件的相关设计方法。仿真和大田试验结果表明，系统温度采集精度最高可达0.01 ℃，测湿精度达±5%，光强采集范围为1~65 535 lx，可并发的数据传输达到36路，可满足农业现场环境数据的较高测量要求。在标称电源供电情况下，系统实际有效生存周期超过142 d，由于采用AT89C51和nRF2401作为基础硬件平台，成本低廉，有助于大规模部署和应用。  相似文献