Likelihood of pregnancy after embryo transfer is reduced in recipient mares with a short preceding oestrus          下载免费PDF全文

J. Cuervo‐Arango  A. N. Claes  M. Ruijter‐Villani  T. A. Stout 《Equine veterinary journal》2018,50(3):386-390

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成年牦牛皮肤中血管与神经的组织学观察及HIF-1α在皮肤中的表达     

任钰昕  崔燕  邹胜南  白雪峰  余四九  何俊峰  廖博 《畜牧兽医学报》2021,52(9):2633-2641

本研究旨在探讨牦牛不同部位皮肤内血管和神经的分布情况,及检测低氧诱导因子-1α（HIF-1α）在不同部位皮肤上的定位及相对表达量,探究牦牛皮肤对高原低氧环境的适应机制。采用HE、Masson’s三色和Verhoeff VG染色法,对成年牦牛皮肤内血管和神经结构进行观察与分析;采用免疫组织化学、实时荧光定量PCR和蛋白免疫印迹法,对HIF-1α的mRNA和蛋白在成年牦牛皮肤组织中表达与分布进行研究。结果表明,颈部血管与神经密度最高,前臂部和小腿部次之,跖部最低,部位间差异显著（P<0.05）。HIF-1α主要表达在表皮层、毛囊的上皮根鞘、皮脂腺、汗腺、血管、神经;颈部、前臂部和小腿部强阳性表达,跖部阳性表达。HIF-1α mRNA的相对表达量跖部明显低于其他部位（P<0.05）,其他三个部位两两比较差异不显著（P>0.05）。HIF-1α蛋白相对表达量颈部最高,跖部最低,差异显著（P<0.05）。研究结果提示,成年牦牛不同部位皮肤内不同血管和神经形态结构相似,密度从颈部到前肢再到后肢差异显著。HIF-1α的差异性表达进一步说明皮肤在牦牛适应低氧环境中发挥作用。  相似文献   

基于物联网的内河小型渔船动态信息监控系统设计   总被引：1，自引：2，他引：1  

贺付亮  李新科  许愿  王曲苑 《农业工程学报》2015,31(20):178-185

为规范内河流域渔业生产秩序,保障渔船作业安全,该文设计了基于物联网的内河小型渔船动态信息监控系统。该系统集成了无线传感器网络、远程信息传输、远端后台监控等多种技术方法。其中,无线传感器网络主要用于获取包括渔船位置、电捕鱼违法监测信息和渔船超载检测信息等渔业现场数据。依靠Zig Bee技术,该网络实现了对不同类别传感器数据的汇聚、判断以及远程播发。远程信息传输是利用GPRS/GSM移动通信网络与互联网传输技术,实现了多渔船作业信息向后台监控中心的实时传输。后台渔政监控中心,具有渔船在电子地图上的识别与定位,渔业生产的实时监控以及渔政执法的决策辅助等功能。该系统经测试,可满足内河流域作业渔船在实时监管和安全保障等方面的需求,提高了农业渔政管理的水平。  相似文献   

组织培养容器环境因子调控技术研究进展   总被引：9，自引：0，他引：9  

何松林  孔德政  杨秋生  孟中生  王美茹  吴建华 《河南农业大学学报》2003,37(1):25-32

综述了组织培养容器中空气环境因子和培养基环境因子调控技术的研究现状，重点介绍了外环境因子中光照强度、光质、照光方式、温度、相对湿度、CO2质量浓度、O2质量浓度、乙烯含量等及内环境因子中的糖含量和培养基支持体等因子对试管苗生长的影响。找出了该方面存在的问题，提出了可能解决的途径。  相似文献   

胚胎移植方法和受体母猪因素对克隆猪生产效率的影响   总被引：2，自引：0，他引：2  

卫恒习  李秋艳  高凤磊  李燕  张守全  李宁 《中国农业科学》2012,45(15):3147-3153

【目的】建立高效的胚胎移植技术体系,以提高克隆猪的生产效率。【方法】比较不同移植方法和不同受体母猪状况的胚胎移植分娩率和克隆猪的出生效率,利用体外发育能力相似的不同品种和发育阶段的克隆胚胎混合移植确定最佳的受体母猪发情同期时间。【结果】克隆胚胎经由输卵管伞移植比输卵管打孔移植具有更高的移植分娩率和克隆猪效率（2.2% 和0.4%,P＜0.01）,而胚胎移植后辅助人工授精会降低克隆效率（0.6% 和 2.2%,P＜0.01）。利用经产母猪作为移植受体比青年猪受体具有更高的克隆效率（3.0%和0.8%,P＜0.01）和平均窝产仔数（（5.5±0.7）头和（2.7±0.3）头,P＜0.05）。受体母猪发情时间比胚胎激活时间晚12-36 h组的克隆效率分别为显著（P＜0.05）和极显著（P＜0.01）,高于晚0 h和早12-24 h 组的克隆效率（2.0%、0.5%和0%）。最佳的受体母猪发情时间为晚于克隆胚胎激活后24 h,克隆效率达3.0%。【结论】选择自然发情时间晚于克隆胚激活后24 h的经产母猪为受体,通过输卵管伞端移植胚胎,能够获得较高的克隆效率,是猪克隆胚胎移植的较理想方法。  相似文献   

海洋捕捞渔船用生物柴油减排的成本-效益分析     

肖晓芸  黄硕琳 《上海海洋大学学报》2013,22(6):949-952

海洋捕捞业是我国渔业能耗和CO2排放的主要领域,捕捞渔船的节能减排水平低,潜力大。总结了我国海洋捕捞渔船能耗及排放现状,分析了生物柴油的特性及作为渔船代用燃料的优缺点,根据海洋捕捞渔船动力装置的特点提出了掺混生物柴油需对柴油机做出的改进措施,对捕捞渔船柴油机掺混5%体积分数的生物柴油的节能减排效果进行了成本-效益分析。结果显示,海洋捕捞渔船柴油机掺混5%的生物柴油将导致每船每年平均减少1 371元左右的燃料费用和2 000元左右的维修费用,但需付出较高的船舶改造成本;若我国所有的海洋捕捞渔船柴油机都添加5%的生物柴油,每年会降低二氧化碳排放近13.4万吨,按目前国际上的碳交易价格计算,每年将产生400多万元的社会环境效益。生物柴油具有一定的经济优势,环保性能显著,具有良好的减排效果,可以促进我国海洋捕捞渔船的节能减排和国家减排目标的实施。  相似文献   

GM-CSF induces human vascular endothelial cells to form vessel-like structure and the role of VEGF     

ZHAO Wei  YANG Xiang-hong  WANG Yan-lin  WANG Yue-zhong  ZHANG Ya-jia 《园艺学报》2007,23(4):682-685

AIM: To investigate the influence of GM-CSF on human vascular endothelial cells induced to form new blood vessels and the role of VEGF. METHODS: HUVECs were cultured by Matrigel to set up a stable angiogenesis system with the stimulating factors. The rhGM-CSF concentration-dependent and time-dependent effects and the role of VEGF165 were detected. CD34 was measured by immunochemical staining and numbers of vessel formation was calculated under microscopic observation. RESULTS: After treatment with rhGM-CSF at various concentrations and at different time points, the numbers of vessel formation increased in a dose-dependent and time-dependent manner. In the presence of VEGF165, the numbers of vessel formation increased evidently. CONCLUSION: HUVECs were induced to develop tubular structure in vitro cultured with Matrigel. GM-CSF promotes human vascular endothelial cells to form vessel-like structure in vitro in a dose-dependent and time-dependent manner. VEGF also in vitro promotes human vascular endothelial cells to form new vessel-like structure.  相似文献   

Myocardial dysfunction resulting from coronary microembolization     

LU Yong-guang  LI Lang 《园艺学报》2007,23(3):619-621

Coronary microembolization is a significant and frequent complication of primary percutaneous coronary intervention in the patients with acute myocardial infarction. More importantly, coronary microembolization is a main reason of dysfunction of microcirculation. The mechanism of myocardial disfunction resulting from coronary microembolition was associated with the vascular active materials released from platelet, platelet aggregation, which aggravate microembolization in microcirculation, endothelial dysfunction and inflammation.  相似文献   

Accounting for unknown foster dams in the genetic evaluation of embryo transfer progeny     

M.J. Surez  S. Munilla  R.J.C. Cantet 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(1):21-29

Animals born by embryo transfer (ET) are usually not included in the genetic evaluation of beef cattle for preweaning growth if the recipient dam is unknown. This is primarily to avoid potential bias in the estimation of the unknown age of dam. We present a method that allows including records of calves with unknown age of dam. Assumptions are as follows: (i) foster cows belong to the same breed being evaluated, (ii) there is no correlation between the breeding value (BV) of the calf and the maternal BV of the recipient cow, and (iii) cows of all ages are used as recipients. We examine the issue of bias for the fixed level of unknown age of dam (AOD) and propose an estimator of the effect based on classical measurement error theory (MEM) and a Bayesian approach. Using stochastic simulation under random mating or selection, the MEM estimating equations were compared with BLUP in two situations as follows: (i) full information (FI); (ii) missing AOD information on some dams. Predictions of breeding value (PBV) from the FI situation had the smallest empirical average bias followed by PBV obtained without taking measurement error into account. In turn, MEM displayed the highest bias, although the differences were small. On the other hand, MEM showed the smallest MSEP, for either random mating or selection, followed by FI, whereas ignoring measurement error produced the largest MSEP. As a consequence from the smallest MSEP with a relatively small bias, empirical accuracies of PBV were larger for MEM than those for full information, which in turn showed larger accuracies than the situation ignoring measurement error. It is concluded that MEM equations are a useful alternative for analysing weaning weight data when recipient cows are unknown, as it mitigates the effects of bias in AOD by decreasing MSEP.  相似文献   

妊娠山羊宫内胎儿脐动脉插管手术方法的研究     

谭 聪杨淑华  何剑斌 《现代畜牧兽医》2014,(3):38-40

为了优化山羊胎儿脐动脉插管模型,本试验采用医用硬膜外导管对单胎妊娠120日龄的山羊进行胎儿脐动脉插管手术,对其手术途径、导管类型、插管方法、麻醉和术后护理进行研究。术后可顺利采集胎儿血液样本,1周后母山羊正常分娩。本试验建立的山羊胎儿脐动脉插管模型的手术方案,可为胎儿疾病的诊断及临床用药等提供试验基础。  相似文献