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蓖麻毒蛋白的改性与抗癌活性 总被引：6，自引：0，他引：6
蒋旭红 《仲恺农业技术学院学报》2006,19(3):60-64
蓖麻毒蛋白对细胞杀伤是非特异性的，因此有严重的毒副作用．文章对蓖麻毒蛋白的改性及改性后的抗肿瘤活性进行了综述，并对由蓖麻毒蛋白构建的免疫毒素在肿瘤的治疗中显示出的良好应用前景进行了讨论． 相似文献
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蓖麻毒素来源广、毒性强、性质稳定,可通过注射、口服、气溶胶等多种方式中毒.为进一步优化蓖麻毒素气溶胶中毒模型,探讨蓖麻毒素气溶胶粒子对气-血屏障、血-胸屏障等免疫屏障的损伤过程,以及实质性免疫器官的病理改变,利用军事医学科学院军事兽医研究所五室已经建立的气溶胶暴露系统,对小鼠进行蓖麻毒素气溶胶的暴露,并于气溶胶暴露后12,30,48 h采集小鼠气管、肺脏、胸腺、脾脏制作病理切片,观察病理变化.结果表明:蓖麻毒素气溶胶粒子能够引起间质性肺炎、脾脏及胸腺细胞变性坏死,从而验证吸入蓖麻毒素气溶胶粒子对免疫屏障的强烈损伤. 相似文献
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Na Dong Zheng Li Qian Li Junhua Wu Peiyuan Jia Yuxia Wang Zhongcai Gao Gang Han Yifan Wu Jianping Zhou Junjie Shan Hua Li Wenqing Wei 《Journal of toxicologic pathology》2014,27(1):73-80
The aim of this work was to investigate the potential interactions between intestinal absorbance and ricin poisoning. The Caco-2 cell monolayer and everted intestinal sac (VEIS) models were used. The distribution of ricin in CD-1 mice intoxicated with 0.1 mg/kg of ricin intragastrically was determined by immunohistochemistry. The results showed that ricin could not transfer across the healthy Caco-2 cell monolayer within three hours after poisoning. However, it could pass through the everted rat intestinal wall after 0.5 h of incubation. The toxin in the liver, spleen, lungs and kidneys of mice could be detected as early as 1 h after intoxication. The pathological results were in accordance with the cytotoxicities of ricin in Caco-2, HepG 2, H1299 and MDCK cells, indicating that though no significant symptom in mice could be observed within 3 h after ricin intoxication, important tissues, especially the kidneys, were being injured by the toxin and that the injuries were progressing. 相似文献
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试验旨在研究蓖麻蛋白对人外周血B淋巴细胞（IM-9）毒性作用及对相关基因表达的影响。将提取的蓖麻蛋白按不同浓度添加到培养基培养6、12 h,研究剂量－时间效应,确定半数抑制浓度,然后以半数抑制浓度培养细胞2、6、10、12 h,在每个时间段检测免疫相关基因CD40、IL-1β、TNF-α和凋亡相关基因Bcl-2、Bax、Caspase-3的表达情况。结果表明,蓖麻蛋白对IM-9细胞的毒性作用随浓度和时间的增加而增强。取接近半数抑制浓度20 ng/mL作用IM-9细胞2、6、10、12 h,免疫相关基因中,TNF-α和CD40基因在6 h试验组表达量与对照组相比显著升高（P<0.05）,10、12 h达到极显著水平（P<0.01）;IL-1β基因表达量试验组、对照组间差异不显著（P>0.05）。凋亡基因中,与对照比相比,试验组Bax基因表达量10 h极显著降低（P<0.01）,12 h显著降低（P<0.05）;Bcl-2基因表达量4个时间段均达到显著水平（P<0.05）,2、12 h达到极显著水平（P<0.01）;Caspase-3基因除6 h表达量降低外,其他时间段表达量均显著升高（P<0.05）,2 h为极显著水平（P<0.01）。表明蓖麻蛋白能显著影响IM-9细胞的基因表达,TNF-α和CD40基因是两个潜在用IM-9细胞评价蛋白毒性的检测基因。 相似文献
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ZHEN Er-dong HUANG Su-juan HUA Chao-ju TAO Cong YANG Shu-lin WANG Yan-fang ZHOU Rong AO Hong LI Kui 《中国畜牧兽医》2016,43(11):2803-2810
This study was amied to learn the toxic effects of ricin on human peripheral blood B lymphocytes（IM-9）and the expression of related genes.The extracted ricin of different concentrations were added to culture cells for 6 and 12 h to make sure the median inhibitory concentration,then culture cells at the median inhibitory concentration for 2,6,10 and 12 h,at each time point detect the expression of immune related genes CD40,IL-1β,TNF-α and apoptosis related genes Bcl-2,Bax,Caspase-3.The results showed that the inhibitory effect of ricin on IM-9 cells was increased with the increase of concentration and time.The expression of TNF-α and CD40 genes increased significantly in experimental group than control group at 6 h（P<0.05).10 and 12 h reached extremely significant level（P<0.01);There was no significance of IL-1β gene expression between experimental and control groups（P>0.05).About apoptosis gene,the Bax gene expression decreased extremely significantly in experimental group at 10 h（P<0.05）and decreased significantly at 12 h（P<0.05);Bcl-2 gene had reached significant levels of four time periods（P<0.05), and 2,12 h were extremely significant（P<0.01);The expression of Caspase-3 reduced at 6 h,the other time points increased significantly（P<0.05),at 2 h reached extremely significant level（P<0.01).It showed that the gene expression of IM-9 cells could be significantly affected by ricin,TNF-α and CD40 genes were two potential genes to evaluate the toxicity by IM-9 cells. 相似文献
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蓖麻饼粕的综合开发与利用 总被引：9，自引：0，他引：9
对蓖麻籽制油后的副 产物－蓖麻饼粕中蓖麻蛋白、一纱以及蓖麻壳的综合开发与利用进行了较全面的综述，并指出了其中存在的问题，探讨了发展方向。 相似文献
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