Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum     

Yuanfa He  Shuyan Chi  Beiping Tan  Xiaohui Dong  Qihui Yang  Hongyu Liu  Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.  相似文献   

极小种群植物“川柿”生物学特性及种子萌发方法研究     

刘燕琴  刘正宇  刘春雷  曹敏  张军 《中国果业信息》2021,50(4)

摘 要：【目的】为了保护和发展极小种群植物川柿，开展此研究。【方法】采用观测法，连续3年对其生物学特性研究；种子萌发方法：（1）后熟0、30、45、60天，剥离清洗播种；（2）后熟60天，以GA300、600、1000 mg/L溶液浸种1h；播种基质为河沙（保持一定湿度），放置室内，统计萌发时间和出苗率。【结果】（1）川柿花期4-5月，10月为果熟期，单果直径32.7±5.80mm，单果重23.0±5.30g；种子肾形、千粒重1070g；成年植株呈隔年开花、结果现象；（2）种子经“后熟+河沙播种”，可萌发，其中以后熟60天的出苗率较高，为65%；直接播种种子，出苗率5%；“后熟+GA溶液浸种+河沙播种”，出苗率均高于65%，其中以GA600mg/L浸泡1h处理，出苗率最高为83.3%。【结论】极小种群植物川柿为常绿叶乔木，有隔年开花、结果现象； “果后熟60天+GA600mg/L浸泡种子1h +室内河沙播种”可提高种子萌发率，为川柿种子繁殖提供参考。  相似文献   

Comparative effect of advanced soy products or corn protein concentrate with porcine meal on growth,body composition,and distal intestine histology of Florida pompano,Trachinotus carolinus     

Romi Novriadi  Elizabeth Spangler  D. Allen Davis 《Journal of the World Aquaculture Society》2019,50(2):433-447

The present study was designed to investigate the effects of diets containing advanced soy products (enzyme‐treated soy and fermented soy) or corn protein concentrate (CPC) in combination with porcine meal (PM) to completely replace poultry byproduct meal (PBM) on growth performance, body composition, and distal intestine histology of Florida pompano, Trachinotus carolinus. Four experimental diets were formulated to be isonitrogenous and isolipidic, to contain 400 g/kg crude protein and 80 g/kg lipid. A reference diet (PBM diet [PBMD]) contained 150 g/kg PBM and 495 g/kg soybean meal (SBM), and three test diets were formulated replacing PBM with 15 g/kg of CPC (CPC diet [CPCD]) or replacing all SBM and PBM with 535 g/kg fermented soy (fermented soybean meal diet [FSBMD]) or 451.3 g/kg enzyme‐treated soy (enzyme‐treated soybean meal diet [ESBMD]). All three test diets were supplemented with 38 g/kg of PM. Diets were fed based on a percentage of bodyweight adjusted after sampling the fish every 2 weeks to triplicate groups of Florida pompano juveniles (mean weight 8.06 ± 0.22 g). After 8 weeks of feeding, fish fed CPCD and ESBMD performed equally well in terms of final body weight, thermal growth coefficient, and percentage weight gain in comparison to fish fed PBMD. In all cases, feeding FSBMD resulted in poor feed conversion and lower feed intake compared to other treatments. Protein retention efficiency, whole‐body proximate composition, phosphorus, sulfur, potassium, magnesium, calcium, sodium, and zinc contents were not significantly influenced by the dietary treatments. The results obtained in the present histological study showed no significant differences in the thickness of serous layer, muscular layer, and submucosal layer of the intestine among treatments. Fish fed CPCD showed a significant widening of the lamina propria with an increase of cellular infiltration and higher presence of goblet cells compared to other dietary treatment. Based on these results, 451 g/kg ESBM or combination of 150 g/kg of CPC and 495 g/kg SBM supplemented with 38 g/kg PM can be utilized to develop a practical diet for juvenile Florida pompano without impacting growth, nutritive parameters, and several distal intestine health parameters.  相似文献   

以塘坝为中心建设立体型综合开发小区     

于明 《中国水土保持》1994,(1):49-50

以塘坝为中心建设立体型综合开发小区于明（黑龙江省青冈县水土保持办公室，１５１６０６）塘坝是我国水利水保建设的重要工程措施之一。它不仅可防洪蓄水，防治水土流失，同时对解决农田抗旱灌溉、养鱼等综合开发利用具有重要作用。搞好以塘坝建设为中心的小流域综合开发...  相似文献   

外源性谷氨酰胺和谷氨酸对早期断奶仔猪肠粘膜形态、结构和小肠吸收功能及骨骼肌中DNA、RNA浓度的影响   总被引：20，自引：0，他引：20  

刘涛  彭健  周诗其  程学慧  陈钢 《中国兽医学报》2003,23(1):62-65

74头28日龄健康二元杂交断奶仔猪分成3组，用于研究外源性谷氨酰胺（glutamine,Gln)和谷氨酰（glutamate, Glu)对断奶仔猪小肠粘膜形态，结构和功能及骨骼肌中DNA，RNA合成的影响。结果表明：与对照组相比，添加1%谷氨酰胺或1%谷氨酸可防止断奶后1周内空肠绒毛萎缩，减少断奶后2周内空肠固有膜内未成熟细胞数；显著改善断奶后1周内小肠吸收功能，并促进肌肉中RNA合成。这些结果为利用Gln、Glu缓解仔猪断奶应激，改善生产性能提供了实验基础。  相似文献   

The Application of GIS in Small Watershed Classification in Loess Plateau     

Zhu Jinzhao  Wu Bin  Bi Huaxing  Zhou Changqing College of Soil  Water Conservation  Beijing Forestry UniversityBeijing  P.R.China 《中国林学(英文版)》1994,(2)

ＴｈｅＡｐｐｌｉｃａｔｉｏｎｏｆＧＩｓｉｎＳｍａｌｌＷａｔｅｒｓｈｅｄＣｌａｓｓｉｆｉｃａｔｉｏｎｉｎＬｏｅｓｓＰｌａｔｅａｕＺｈｕＪｉｎｚｈａｏ，ＷｕＢｉｎ，ＢｉＨｕａｘｉｎｇ，ＺｈｏｕＣｈａｎｇｑｉｎｇＣｏｌｌｅｇｅｏｆＳｏｉｌａｎｄＷａｔｅｒＣ...  相似文献   

Histopathology of Experimental Schistosoma bovis Infection in Goats     

R. Lindberg  J. Monrad  M. Vang Johansen  N. . Christensen  P. Nansen 《Acta veterinaria Scandinavica》1993,34(2):183

The inflammatory host response to Schistosoma bovis in young goats was studied at necropsy by light microscopy 34 weeks after primary exposure to 3,000 cercariae (group B, n=6), 34 weeks after primary exposure to 3,000 cercariae followed by challenge with 2,500 cercariae at week 17 (group C, n=5), and 17 weeks after primary exposure to 2,500 cercariae, given on week 17 of the experiment (group D, n=6). Three goats served as uninfected controls. The faecal egg output had been minimal for 17 weeks prior to necropsy in groups B and C and only for the last 2 weeks in group D.Histological studies were carried out on the small intestine, liver, lung and spleen, and tissue egg counts were performed. In sections of the small intestine and liver, a panel of histopathological variables were quantitated to characterize the host response and differences between groups of animals were evaluated with one way analysis of variance. The mean tissue egg count in the small intestine was slightly but not significantly higher in group C than group B and about twice as high in group D (D vs B or C p<0.01). Group means of numbers of inflammatory foci per section of gut wall corresponded well with those of tissue egg counts, suggesting that the rate of inflammatory destruction of eggs did not differ markedly between the groups. Egg material was less commonly seen in granulomas of the small intestine in group B than in group D (p<0.01), suggesting lower passage of eggs through the gut wall during the later than during the earlier phase of patent primary infection. The frequency of eosinophil-rich hepatic inflammatory foci was much higher in group D than in the other groups (D vs B p<0.05, D vs C p< 0.01), and coincided with a high degree of blood eosinophilia in this group at the time of sacrifice. Challenged goats showed a significantly higher frequency of markedly fibrotic inflammatory foci in the liver and of liver granulomas with a marked giant cell component than goats of the other groups. Hepatic portal fibrosis was least prominent in animals with 17- week- old primary infections, implying a possible relation between this change and duration of infection.  相似文献   

对小水电、沼气池、省柴灶三个能源工程项目的综合评价   总被引：1，自引：0，他引：1  

曾晶 《贵州农业科学》2005,33(4):65-67

农村能源工程项目的建设不仅具有项目本身的经济效益，而且有更广泛的能源、间接的经济和生态效益。农村能源工程项目的建设除了受资金约束外，还有技术管理等方面的限制，甚至人们的习俗和心理状态都可能是能源工程项目的制约因素。因此，对农村能源工程项目的多种效益和多种制约的综合分析和评价，将使我们能更全面地考虑项目的综合效果，使有限的资金、人力、物力投入到综合效果最好的项目上去。  相似文献   

新疆水土保持情况调查     

郭廷辅  佟伟力 《中国水土保持》1994,(12):5-8

新疆水土保持情况调查郭廷辅，佟伟力（水利部水土保持司，北京１００７６１）我们于１９９４年８月１１日至２６日对新疆伊犁、和田、喀什、吐鲁番、昌吉、乌鲁木齐等６个地区（州、市）的１１个县（市）、两个流域的水土流失情况和开展治理工作做了典型调查。同时，也对...  相似文献   

杂交条纹鲈苗种小水泥池培育技术试验     

田功太段登选  曹振杰马荣棣  陈述江孙栋 陈金萍 《齐鲁渔业》2004,21(7):1-3

用地上露天小水泥池8个．进行杂交条纹鲈苗种培育试验．放养313龄水花30000尾．通过天然饵料、藻粉投喂以及适当水质管理措施．经32天培育，共培育3．5～4．6cm体长的鱼种4336尾．最高成活率为19．1％，最低为6％，平均为14．5％。  相似文献