Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity     

Anguo Li  Ruihao Huang  Chaogang Wang  Qunju Hu  Hui Li  Xiao Li 《Marine drugs》2021,19(5)

Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides.  相似文献   

以塘坝为中心建设立体型综合开发小区     

于明 《中国水土保持》1994,(1):49-50

以塘坝为中心建设立体型综合开发小区于明（黑龙江省青冈县水土保持办公室，１５１６０６）塘坝是我国水利水保建设的重要工程措施之一。它不仅可防洪蓄水，防治水土流失，同时对解决农田抗旱灌溉、养鱼等综合开发利用具有重要作用。搞好以塘坝建设为中心的小流域综合开发...  相似文献   

The Application of GIS in Small Watershed Classification in Loess Plateau     

Zhu Jinzhao  Wu Bin  Bi Huaxing  Zhou Changqing College of Soil  Water Conservation  Beijing Forestry UniversityBeijing  P.R.China 《中国林学(英文版)》1994,(2)

ＴｈｅＡｐｐｌｉｃａｔｉｏｎｏｆＧＩｓｉｎＳｍａｌｌＷａｔｅｒｓｈｅｄＣｌａｓｓｉｆｉｃａｔｉｏｎｉｎＬｏｅｓｓＰｌａｔｅａｕＺｈｕＪｉｎｚｈａｏ，ＷｕＢｉｎ，ＢｉＨｕａｘｉｎｇ，ＺｈｏｕＣｈａｎｇｑｉｎｇＣｏｌｌｅｇｅｏｆＳｏｉｌａｎｄＷａｔｅｒＣ...  相似文献   

消失模铸造技术在大规模生产中的应用     

焦龙钢 《拖拉机与农用运输车》2005,(6):85-87

针对铸件特点,采用国内外的先进技术,实现了消失模铸造大批量流水线生产。  相似文献   

新疆水土保持情况调查     

郭廷辅  佟伟力 《中国水土保持》1994,(12):5-8

新疆水土保持情况调查郭廷辅，佟伟力（水利部水土保持司，北京１００７６１）我们于１９９４年８月１１日至２６日对新疆伊犁、和田、喀什、吐鲁番、昌吉、乌鲁木齐等６个地区（州、市）的１１个县（市）、两个流域的水土流失情况和开展治理工作做了典型调查。同时，也对...  相似文献   

杂交条纹鲈苗种小水泥池培育技术试验     

田功太段登选  曹振杰马荣棣  陈述江孙栋 陈金萍 《齐鲁渔业》2004,21(7):1-3

用地上露天小水泥池8个．进行杂交条纹鲈苗种培育试验．放养313龄水花30000尾．通过天然饵料、藻粉投喂以及适当水质管理措施．经32天培育，共培育3．5～4．6cm体长的鱼种4336尾．最高成活率为19．1％，最低为6％，平均为14．5％。  相似文献   

小流域治理成果的开发利用途径     

李志正  雷升文 《中国水土保持》1994,(1):46-48

根据市场经济的要求和目前小流域治理中普遍存在的高投入、低效益问题，提出以市场经济为导向，通过合理开发利用治理成果，优化组合资源、资金、劳力、技术等生产要素，努力发展商品生产，提高小流域治理的经济效益。结合各地实践，总结出基本农田的集约经营、水保林的经营增值、水保草的养畜转化、非生产地的开发利用、转化产品的加工增值和疏通流通渠道六条开发利用途径。  相似文献   

Expression of a recombinant crustacean hyperglycemic hormone of the kuruma prawn Penaeus japonicus in methylotrophic yeast Pichia pastoris     

TSUYOSHI  OHIRA  HIDEKAZU  KATAYAMA  KATSUMI  AIDA  HIROMICHI  NAGASAWA 《Fisheries Science》2003,69(1):95-100

ABSTRACT:   Until now, six crustacean hyperglycemic hormones (CHH) designated Pej-SGP-I, -II, -III, -V, -VI and -VII have been characterized in the kuruma prawn Penaeus japonicus . All CHH consist of 72 amino acid residues and have an amidated carboxyl (C)-terminus. In the present study, we expressed Pej-SGP-III in methylotrophic yeast Pichia pastoris in order to obtain a large quantity of recombinant CHH possessing biological activity. A cDNA encoding Pej-SGP-III that had been previously cloned was processed by polymerase chain reaction (PCR) and the resulting product was ligated into an expression vector. Pichia pastoris was transformed with this vector after which a recombinant Pej-SGP-III was expressed having an additional amino acid residue (glycine) at the C-terminus (rPej-SGP-III-Gly), a form considered to be a putative precursor of this hormone. rPej-SGP-III-Gly secreted into the culture medium was purified by reversed-phase HPLC, and amidated using a peptidylglycine alpha-amidating enzyme. The amidated rPej-SGP-III (rPej-SGP-III) showed hyperglycemic activity in in vivo bioassay almost comparable to that of the natural Pej-SGP-III. rPej-SGP-III thus obtained will be a useful tool not only for its physiological study but also for the determination of its 3-D structure.  相似文献   

实验性流行性腹泻仔猪小肠粘膜上皮细胞及肠系膜淋巴结的超微结构     

田欣田  郑兆荣  邱震东  刘宝岩  杨盛华 《中国兽医学报》1988,(2)

给8头生后3d的哺乳仔猪经口感染猪流行性腹泻病毒(PEDV)“吉”毒株,于感染后18、30、45和96h各扑杀2头,以透射电镜和扫描电镜观察了小肠粘膜上皮细胞及肠系膜淋巴结的超微结构。结果表明,小肠上皮细胞的病变因感染时间不同而有明显差异。上皮细胞的脱落和残留上皮细胞超微结构的破坏,以感染后30h最严重,病毒在这些上皮细胞内的增殖最显著。感染后45h,见有大量新生上皮细胞修补损伤的肠绒毛。感染后96h,小肠绒毛短缩、粗大乃至发生融合。实验仔猪肠系膜淋巴结内巨噬细胞和淋巴细胞的超微结构均遭到破坏,在巨噬细胞内见有PED冠状病毒粒子。  相似文献   

Use of space by bank voles (Clethrionomys glareolus) in a polish farm landscape     

Michŀ Kozakiewicz  Anna Kozakiewicz  Aleksy Łukowski  Tomasz Gortat 《Landscape Ecology》1993,8(1):19-24

Movements of bank voles (Clethrionomys glareolus) were studied in a farmland mosaic in Poland. Distances crossed by animals in short-time periods are significantly longer in heterogenous than in homogenous habitats. In long-time and large-spatial scales, a significant portion of the animals in a population travel among habitat elements of the mosaic, reducing the degree of isolation of patch populations and decreasing the probability of local extinction.  相似文献