用台盼兰—姬姆萨染色检测家畜精子顶导反应的研究   总被引：1，自引：0，他引：1  

张嘉保  董伟 《黑龙江畜牧兽医》1992,(1):6-10

本文探讨了用台盼兰—姬姆萨染色检测家畜精子顶体反应的可行性。用肝素或钙离子载体诱发精子顶体反应。根据染色结果将精子分为四类:a)核后帽部不着色或淡青色,顶体不着色或部分紫红色(有顶体反应活精子);b)核后帽部暗青色.顶体部不着色或部分暗红色(有顶体反应死精子);c)核后帽部不着色或淡青色,顶体部紫红色(无顶体反应活精子);d)核后帽部暗青色,顶体部暗紫红色(无顶体反应死精子)。有顶体反应活精子百分率与仓鼠卵穿透率呈强正相关。从而证明台盼兰—姬姆萨染色是检测家畜精子顶体反应的有效手段,并能预测获能处理后精子的受精能力。  相似文献   

丝核菌细胞核染色技术的研究   总被引：16，自引：0，他引：16  

陈平  余土元  赵玉环  陈惠阳 《仲恺农业技术学院学报》2001,14(4):13-17

比较了5种不同的染色方法对丝核菌（Rhizoctonia)菌株细胞核的染色效果。结果表明，番红O－KOH的染色效果最好，Giemsa、苯胺蓝－甘油次之，染色效果最差的是苯胺蓝－乳酚油及曲利苯蓝－乳酚油。用番红O－KOH染色方法对分离自13种作物的57个丝核菌菌株进行细胞核染色，其中3个菌株为双核丝核菌，其余54个菌株为多核丝核菌。  相似文献   

家兔黏膜免疫器官肥大细胞在巴氏杆菌感染中的特征——组织化学与电镜观察   总被引：3，自引：0，他引：3  

呼格吉乐图  佘锐萍  刘玉峰  马卫民  胡艳欣 《中国预防兽医学报》2006,28(4):419-422

对5例巴氏杆菌感染家兔的空肠与圆小囊肥大细胞（mast cell，MC）进行了组织化学与电镜的观察，发现在细菌感染导致的病变组织边缘、病变组织内血管附近结缔组织中及变性细胞周围组织的上皮下与黏膜下层都出现了大量甲苯胺蓝（Toluidine Blue，TB）阳性肥大细胞。电镜下，MC有的直接与变性细胞密切接触，有的则和血管内皮紧密相触，在此部位的血管内可见有淋巴细胞贴附填充。MC胞浆内充盈大量的特征性颗粒，有时这些颗粒向细胞表面突出形成边缘空隙，即形成所谓的脱颗粒管道，将颗粒内容物逐渐排除细胞外，遗留空腔。  相似文献   

Expression of the tobacco β-1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina     

Deanna L. Funnell  Christopher B. Lawrence  Jeffrey F. Pedersen  Christopher L. Schardl 《Physiological and Molecular Plant Pathology》2004,65(6):285-296

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR.  相似文献   

A fast,low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species          下载免费PDF全文

BR Rui  DSR Angrimani  LC Bicudo  JDA Losano  M Nichi  RJG Pereira 《Reproduction in domestic animals》2018,53(1):171-175

Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high‐precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue‐stained spermatozoa were considered to have DNA fragmentation. We observed high linear regression coefficients between the expected proportion of damaged DNA and the results of TB for dog, cat, ram, bull and stallion samples. In conclusion, TB stain was considered a fast and effective technique for the study of spermatozoa DNA in several species.  相似文献   

高致病性猪蓝耳病防控     

马海东 《畜牧兽医科学(电子版)》2021,(5):151-152

随着人们生活方式的变化,养猪户也变得越来越多,不仅能为其带来更多的经济收入,也可提高其生活质量和水平。调查数据显示,很多养殖户在养猪中通常会因很多因素致使猪群感染疾病,倘若不能及时处理,势必会对自身带来较为严重的经济损失,甚至会加大疫病的扩散。该文主要对高致病性猪蓝耳病防控新理念展开分析,并提出一些可行的对策。  相似文献   

Effects of Different Red and Blue Ratios on the Somatic Embryogenesis and Plant Regeneration of Cotton   总被引：1，自引：0，他引：1  

WEI Xi  WANG QianHua  GE XiaoYang  CHEN YanLi  DING YanPeng  ZHAO MingZhe  LI FuGuang 《中国农业科学》2019,52(6):968-980

  相似文献   

钼蓝比色法测定笃斯越橘成熟果实中还原型抗坏血酸含量及体系优化   总被引：1，自引：0，他引：1  

谭智文  宋春艳  郑美香  崔百会  宗成文 《湖北农业科学》2015,(7)

以笃斯越橘(Vaccinium uliginosum)成熟果实为试材,以钼蓝比色法测定笃斯越橘果实还原型抗坏血酸(Reduced ascorbic acid,As A)含量进行条件优化。结果表明,3%偏磷酸-乙酸用量为100μL、5%硫酸和5%钼酸铵用量均为200μL、以去离子水补充至1 500μL,30℃干热恒温浴显色40 min,取出室温下放置1 h后,在700 nm下测定,所得数据稳定,准确性适合用于笃斯越橘果实As A含量的测定;测得含量为(40.20±6.23)mg/100 g FW。  相似文献   

Diel differences in blue whale (Balaenoptera musculus) dive behavior increase nighttime risk of ship strikes in northern Chilean Patagonia     

Francesco CARUSO  Leigh HICKMOTT  Joseph D. WARREN  Paolo SEGRE  Gustavo CHIANG  Paulina BAHAMONDE  Sonia ESPAÑOL-JIMÉNEZ  Songhai LI  Alessandro BOCCONCELLI 《Integrative zoology》2021,16(4):594-611

The northern Chilean Patagonia region is a key feeding ground and a nursing habitat in the southern hemisphere for blue whales (Balaenoptera musculus). From 2014 to 2019, during 6 separate research cruises, the dive behavior of 28 individual blue whales was investigated using bio-logging tags (DTAGs), generating ≈190 h of data. Whales dove to significantly greater depths during the day compared to nighttime (day: 32.6 ± 18.7 m; night: 6.2 ± 2.7 m; P < 0.01). During the night, most time was spent close to the surface (86% ± 9.4%; P < 0.01) and at depths of less than 12 m. From 2016 to 2019, active acoustics (scientific echosounders) were used to record prey (euphausiids) density and distribution simultaneously with whale diving data. Tagged whales appeared to perform dives relative to the vertical migration of prey during the day. The association between diurnal prey migration and shallow nighttime dive behavior suggests that blue whales are at increased risk of ship collisions during periods of darkness since the estimated maximum ship draft of vessels operating in the region is also ≈12 m. In recent decades, northern Chilean Patagonia has seen a large increase in marine traffic due to a boom in salmon aquaculture and the passenger ship industry. Vessel strike risks for large whales are likely underestimated in this region. Results reported in this study may be valuable for policy and mitigation decisions regarding conservation of the endangered blue whale.  相似文献   

Observations on the bismuth sulfide colorimetric procedure for sulfate analysis in soil     

《Communications in Soil Science and Plant Analysis》2012,43(1):79-86

Abstract

The bismuth sulfide and methylene blue colorimetric finishes for soil sulfate determination by the Johnson‐Nishita distillation method were examined and compared. Both procedures gave comparable results when applied to soils and soil extracts, but the bismuth method was more rapid and more reliable. Nitrogen flow rate over the range 100–500 ml/min was not critical for the bismuth method, provided appropriate distillation times were employed. However, the release and transfer of hydrogen sulfide in the bismuth method was effected in one‐third of the time required for the méthylène blue finish, largely due to the elimination of a gas‐washing step. Predrying of soil extract samples increased recovery of hydrogen sulfide with the bismuth procedure arid could be used to increase sensitivity. Nitrate interference was observed with the bismuth procedure but attributed to interference during the reduction step. The bismuth procedure can readily be adapted for microdetermination in the 0–40 ppm sulfate‐S range.  相似文献