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三丁酸甘油酯对乙酸刺激仔猪肠道屏障功能的影响 总被引：1，自引：1，他引：0
本试验旨在研究三丁酸甘油酯（TB）对乙酸（ACA）刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪（杜×长×大）,随机分为3个处理：对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21 d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1% TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10 mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶（DAO）活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明：①未用乙酸刺激前,与对照组相比,日粮中添加0.1% TB对仔猪平均日增重有一定程度提高（P=0.089）,且显著降低了仔猪料重比（P<0.05）;②与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积（P<0.05）;③与乙酸组相比,TB组显著降低了血浆DAO活力（P<0.05）,极显著降低了回肠AREG基因的相对表达量（P<0.01）。综合上述结果,日粮中添加0.1% TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。 相似文献
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Yanbin Hou Yong Hou Lei Yao Sha Chen Jinghui Fan Lichun Qian 《Aquaculture Research》2019,50(3):836-846
A 60‐day feeding trial was carried out to evaluate the effects of chromium yeast (CY), tributyrin (TB) and bile acid (BA) on the snakehead (Channa argus). The control (CT) group was fed the basal diet, and the experimental groups were fed 200 mg/kg CY (concentration: 1,000 mg/kg), 2.5 g/kg TB (purity: 45%) or 1 g/kg BA (purity: 30%) in addition to the basal diet. The addition of CY, TB and BA statistically increased the weight gain rate, and specific growth rate, whereas the feeding rate and feed conversion ratio were significantly reduced (p < 0.05). When compared with the CT group, TB elevated the serum glutathion peroxidase (GSH‐Px) activity; and BA reduced low density lipoprotein cholesterol (LDL‐C) contents significantly (p < 0.05). The three feed additives reduced intestinal malonaldehyde (MDA) levels dramatically. Tributyrin significantly enhanced intestinal trypsin and pepsin activities (p < 0.05). The activity of fructose‐1,6‐bisphosphatase and pyruvate carboxylase in the group fed CY was significantly lower than in the CT group. In conclusion, dietary inclusion of the three additives enhanced growth performance of C. argus. Dietary supplementation of CY affected protein, glucose and lipid metabolism. Diets with TB levels improved intestinal digestive enzyme activity and antioxidant capacity. The addition of BA improved glycolipid metabolism. 相似文献
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为了了解米酒乳杆菌在脂类环境中的生存状况,为改进发酵剂和发酵产品提供理论基础,用定量PCR方法对米酒乳杆菌La22在橄榄油和三丁酸甘油培养基中生长时生境适应相关基因表达进行分析。结果显示,与对照相比,无论是在添加橄榄油还是添加三丁酸甘油的MRS培养基中,米酒乳杆菌中与精氨酸脱亚氨酶途径(ADI)途径相关的6个arc操纵子基因的表达量下调十分明显,且在橄榄油中arc操纵子基因的表达量均低于其在含三丁酸甘油培养基中的表达量;压力相关基因usp5在三丁酸甘油培养基中的表达量明显下调,usp2、usp4、lsa0169和lsa0170在2种培养基中的表达量均明显下调,lsa01079的表达量在三丁酸甘油培养基中明显上调,lsa0674和lsa0697在2种培养基中的表达量均明显上调;应激基因lsa1152_b在橄榄油和三丁酸甘油培养基中的表达量明显上调,而lsa1111、lsa1112、lsa0552和lsa0619基因的表达量在三丁酸甘油培养基中明显下调。结果表明米酒乳杆菌能适应脂类丰富的培养环境,但需进一步研究来证实米酒乳杆菌对各种脂类环境的适应性。 相似文献
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Peng Tan Xun Wu Wenliang Zhu Bao Lou Ruiyi Chen Ligai Wang 《Aquaculture Research》2020,51(5):2004-2019
Six equal‐protein and equal‐lipid diets were formulated: the fish meal (FM) diet, the soya bean meal (SBM) diet with 40% of FM protein replaced by SBM protein and tributyrin (TB) diets with 0.05% (TB0.05), 0.10% (TB0.1), 0.20% (TB0.2) and 0.40% (TB0.4) tributyrin supplemented in the SBM‐based diet. Each kind of diet was randomly fed to triplicate tanks with 20 fish per tank. Fish were fed apparent satiation twice daily for 56 days. No significant difference in weight gain rate (WGR) and feed efficiency rate (FER) was observed between fish fed the FM, TB0.1 and TB0.2 diets (p > .05). Muscle histidine and arginine proportion of fish fed TB0.1 diet was significantly higher than that of fish fed the SBM diet (p < .05). Intestine morphology results indicated that the supplementation of 0.1% tributyrin significantly improved the mucosal fold height, microvilli length and microvilli density when compared with those of fish fed the SBM diet (p < .05). The supplementation of dietary tributyrin suppressed the pro‐inflammatory gene expression, which may be due to the improvement of physical barrier and modification of microbial communities, such as Acinetobacter, Rhodocyclaceae, Brevundimonas, Sphingopyxis, Hydrogenophaga, Methyloversatilis and Devosia. In conclusion, dietary 0.1% tributyrin supplementation in high‐soya bean meal diet improved growth performance, flesh quality and intestinal morphology structure integrity of yellow drum. 相似文献
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本试验旨在研究饲粮添加不同水平三丁酸甘油酯对断奶仔猪生长性能、血清生化指标、肠组织形态和养分消化率的影响。选取平均体重为7.66 kg的21日龄“杜×长×大”断奶仔猪200头,随机分为5组,每组5个重复,每个重复8头猪。对照组饲喂添加40 mg/kg杆菌肽锌的基础饲粮,试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组在基础饲粮中分别添加1000、1500、2000、2500 mg/kg三丁酸甘油酯,试验期为42 d。结果表明:与对照组相比,饲粮中添加2000和2500 mg/kg三丁酸甘油酯显著提高了仔猪平均日增重(P<0.05),显著降低了料重比(P<0.05),显著增加了血清中免疫球蛋白M含量(P<0.05);饲粮中添加1500、2000、2500 mg/kg三丁酸甘油酯显著提高了仔猪血清葡萄糖和总蛋白含量(P<0.05),显著提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度(P<0.05),显著提高了仔猪对饲粮中粗蛋白质、粗脂肪、钙和磷的表观消化率(P<0.05),显著降低了血清中球蛋白含量和二胺氧化酶活性(P<0.05);饲粮中添加1000 mg/kg三丁酸甘油酯显著提高了血清中总蛋白含量(P<0.05),显著降低了血清中二胺氧化酶活性(P<0.05),显著提高了仔猪对饲粮中粗蛋白质和粗脂肪的表观消化率(P<0.05)。由此可见,仔猪饲粮中添加1000~1500 mg/kg三丁酸甘油酯可替代抗生素药物添加剂;饲粮中添加2000~2500 mg/kg三丁酸甘油酯不仅可替代抗生素药物添加剂,而且可显著提高仔猪生长性能,改善肠组织形态,增强免疫力,提高养分消化率。 相似文献
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为研究草鱼饲料中添加三丁酸甘油酯对其鱼体成分、形体指标及肠道消化酶的影响。选取健康的草鱼幼鱼(20.04 g±0.19 g)随机分为4组,每组设置3个重复,分别投喂基础日粮(T1)以及基础日粮中添加300 mg/kg(T2)、600 mg/kg(T3)、1 000 mg/kg(T4)三丁酸甘油酯饲粮,在水库网箱中进行8周的养殖试验。结果表明,T4组的鱼体粗蛋白含量较基础日粮组(T1)有提高的趋势(P>0.05),水分含量显著降低(P<0.05),各组之间的粗脂肪和粗灰分含量无显著差异(P>0.05)。与基础日粮组相比,T2、T3和T4组的肝体比显著提高,T2组的肠体比显著提高(P<0.05)。T3和T4组的肥满度、脏体比、肠体比和肠长比均有上升的趋势(P>0.05)。T3组肠道脂肪酶活力较T1组显著提高,T4组的淀粉酶活力显著提高(P<0.05)。综上可得,饲料中添加三丁酸甘油酯能够改变草鱼幼鱼的鱼体成分和形体指标,提高肠道消化酶活力。 相似文献
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Effects of tributyrin supplementation on ruminal microbial protein yield,fermentation characteristics and nutrients degradability in adult Small Tail ewes 
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下载免费PDF全文 Qing‐Chang Ren Jing‐Jing Xuan Li‐Ke Wang Qiu‐Wen Zhan Ding‐Zhong Yin Zhong‐Ze Hu Hong‐Jian Yang Wei Zhang Lin‐Shu Jiang 《Animal Science Journal》2018,89(9):1271-1279
Two trials were conducted to assess the effects of tributyrin (TB) supplementation on ruminal microbial protein yield and fermentation characteristics in adult sheep. In an in vitro trial, substrate was made to offer TB at 0, 2, 4, 6, and 8 g/kg on a dry matter (DM) basis and incubated for 48 hr. In an in vivo trial, 45 adult ewes were randomly assigned by initial body weight (55 ± 5 kg) to five treatments of nine animals over an 18‐day period. Total mixed ration was made to offer TB to ewes at 0, 2, 4, 6, and 8 g/kg on a DM basis. The in vitro trial showed that TB enhanced apparent degradation of DM (p = .009), crude protein (p < .001), neutral detergent fiber (p = .007) and acid detergent fiber (p = .010) and increased methanogenesis (p < .001), respectively. The in vivo trial showed that TB decreased DM intake (p < .001) and enhanced rumen microbial N synthesis (p < .001), respectively. Both in vitro and in vivo trials showed that TB increased total volatile fatty acid concentration and enhanced fibrolytic enzyme activity. The results indicated that TB might exert positive effects on microbial protein yield and fermentation in the rumen. 相似文献
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Yudai Inabu Kyotaro Murayama Katsutoshi Inouchi Toshihisa Sugino 《Animal Science Journal》2019,90(9):1185-1192
The objective of this study was to evaluate the effect of tributyrin (TB) supplementation to milk replacer (MR) on performance, health, and blood concentrations of metabolite and glucagon‐like peptide (GLP‐2) in pre‐weaning calves. Twenty Holstein heifer calves were raised on an intensified nursing program using MR supplemented with either palm oil (CON) or TB (TB) at 0.3% (as fed basis) for 7 weeks starting 1 week after birth. Calves were fed a calf starter and kleingrass from the beginning of the study. Blood samples were obtained weekly to measure blood glucose, serum β‐hydroxybutyric acid (BHBA), insulin‐like growth factor 1 (IGF‐1), and plasma GLP‐2 concentrations. Starter DMI and metabolizable energy (ME) intake were lower in TB calves at 46, 47, from 49 to 55 days after birth compared with the CON calves. However, any growth parameters were not affected by TB treatment. Blood glucose, serum BHBA, and IGF‐1 concentrations were not affected by TB supplementation. On the other hand, mean plasma GLP‐2 concentration among whole experimental period was higher for TB (0.60 ng/ml) compared with CON (0.41 ng/ml). In conclusion, feeding MR supplemented with TB increases plasma GLP‐2 concentration, which might counterbalance the growth performance of TB calves despite the decreased ME intake. 相似文献
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三丁酸甘油酯对断奶仔猪生长性能及养分消化率的影响 总被引：1，自引：0，他引：1
为研究三丁酸甘油酯对断奶仔猪生长性能及养分消化率的影响,选用96头健康、平均体重为（7.93±0.04） kg的长大二元杂种断奶仔猪随机分为2组,每组6栏,每栏8头,对照组饲喂基础饲粮,三丁酸甘油酯组饲喂基础饲粮＋0.20%三丁酸甘油酯。预试期3 d,正试期21 d。结果显示,与对照组相比,三丁酸甘油酯组仔猪日采食量提高7.02%（P〉0.05）,日增重提高11.79%（P〈0.05）,料重比降低4.14%（P〉0.05）,腹泻率和发病率分别降低66.67%（P〈0.01）和22.22%（P〈0.05）。饲粮粗蛋白质、粗脂肪和能量的表观消化率分别提高6.56%（P〈0.05）、12.38%（P〈0.05）和4.09%（P〈0.05）。说明饲粮中添加0.20%三丁酸甘油酯可提高断奶仔猪生长性能和饲粮养分消化率。 相似文献
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Josie Ancella Volatiana Lei Wang Neveen Gray Shengli Tong Gangwei Zhang Qingjun Shao 《Aquaculture Research》2020,51(1):135-146
An 8‐week growth trial was conducted to evaluate the effects of different levels of tributyrin supplementation in a high‐soya bean meal diet on juvenile black sea bream (11.30 ± 0.16 g). The positive control (PC) diet contained 45% fishmeal and 20% soya bean meal, while the negative control (NC) contained 12% fishmeal and 45% soya bean meal. Graded levels of tributyrin were added to the NC diet at 0.05% (TB 0.05), 0.1% (TB 0.1), 0.2% (TB 0.2), 0.4% (TB 0.4) and 0.8% (TB 0.8). Ultimately, the fish fed the PC diet had a higher weight gain and specific growth rate than the fish fed other diets. The fish fed the NC diet had the lowest growth, and TB 0.05–TB 0.2 diets increased growth performance while TB 0.4–TB 0.8 diets caused reduction in growth. Dietary tributyrin supplementation improved protease activity and enhanced antioxidant capacity. Compared with the fish fed the NC diet, the fish fed the tributyrin‐supplemented diets had improved gut morphology and structure, and the results were similar to those of the fish fed the PC diet. Furthermore, the analysis of the dose response with second‐order polynomial regression indicated that the optimum tributyrin supplementation for juvenile black sea bream is 2.24 g/kg in the 45% soya bean meal diet. 相似文献
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