Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture          下载免费PDF全文

T Nongbua  Y Guo  A Edman  P Humblot  JM Morrell 《Reproduction in domestic animals》2018,53(1):85-92

The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0–13 × 10⁵ cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent.  相似文献   

Mare and foal survival and subsequent fertility of mares treated for uterine torsion          下载免费PDF全文

T. J. P. Spoormakers  E. A. M. Graat  F. ter Braake  T. A. E. Stout  H. J. Bergman 《Equine veterinary journal》2016,48(2):172-175

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一例布偶猫分娩期单子宫扭转破裂的病例报告     

杨金福 《畜牧兽医杂志》2024,43(1):138-139

一只2岁布偶猫,体重4.3 kg,怀孕61 d,精神不好,血常规检查严重贫血,腹部彩超检查死胎、子宫扭转、子宫破裂、血腹,诊断为子宫扭转破裂。母猫交叉配血后输血60 mL,呼吸麻醉,行卵巢子宫切除术,术后消炎止血,1周拆线恢复。  相似文献   

Laboratory study of SO2 dry deposition on limestone and marble: Effects of humidity and surface variables     

E. C. Spiker  R. P. Hosker Jr.  V. C. Weintraub  S. I. Sherwood 《Water, air, and soil pollution》1995,85(4):2679-2685

The dry deposition of gaseous air pollutants on stone and other materials is influenced by atmospheric processes and the chemical characteristics of the deposited gas species and of the specific receptor material. Previous studies have shown that relative humidity, surface moisture, and acid buffering capability of the receptor surface are very important factors. To better quantify this behavior, a special recirculating wind tunnel/environmental chamber was constructed, in which wind speed, turbulence, air temperature, relative humidity, and concentrations of several pollutants (SO₂, O₃, nitrogen oxides) can be held constant. An airfoil sample holder holds up to eight stone samples (3.8 cm in diameter and 1 cm thick) in nearly identical exposure conditions. SO₂ deposition on limestone was found to increase exponentially with increasing relative humidity (RH). Marble behaves similarly, but with a much lower deposition rate. Trends indicate there is little deposition below 20% RH on clean limestone and below 60% RH on clean marble. This large difference is due to the limestone's greater porosity, surface roughness, and effective surface area. These results indicate surface variables generally limit SO₂ deposition below about 70% RH on limestone and below at least 95% RH on marble. Aerodynamic variables generally limit deposition at higher relative humidity or when the surface is wet.  相似文献   

论犬子宫破裂     

王艳红  杨荣  纪海青 《北京农业》2012,(15):121-122

犬的子宫体部或子宫角在妊娠期、分娩期或子宫发生异常病变时,发生破裂称为子宫破裂。子宫破裂为生殖系统最严重并发症之一,常引起母体和胎儿的死亡。  相似文献   

Morphologic evaluation of acute endometritis in mares with differing resistance to uterine infections     

T Katila  T F Lock  R W Ely  A R Smith 《Acta veterinaria Scandinavica》1986,27(3):378-387

The study was designed to determine differences between normal mares and mares with endometrial pathology in the inflammatory response after bacterial challenge. Six normal mares (biopsy category I) and 4 mares with pathological endometrial changes (biopsy category II) were given an intrauterine infusion of β-hemolytic streptococci on the second day of estrus. All mares had a similar kind of inflammatory response after the bacterial inoculation as assessed by rectal and vaginal examinations. There were no significant differences in the amount of discharge, uterine tone, uterine size and cervical relaxation between the groups. Leukocytic response, as determined by endometrial smears and biopsies, was of the same magnitude in both groups. Two mares from the pathological group were not able to eliminate the infection, but had vaginal discharge and bacteriologically positive uterine swabs until the end of the experiment. It is concluded that the inability of some mares to clear uterine infections cannot be explained by a deficient inflammatory response.  相似文献   

RADIOGRAPHIC EVALUATION OF THE UTERUS: A REVIEW     

Norman  Ackerman  Dvm 《Veterinary radiology & ultrasound》1981,22(6):252-257

Radiographic evaluation of uterine enlargement is discussed and illustrated. Normal (pregnancy) and abnormal (pyometra, complications of pregnancy, neoplasma) causes of uterine enlargement are addressed. Special radiographic procedures for uterine evaluation are described.  相似文献   

Uterine Activity after Induction of Hypocalcaemia in the Ovariectomized Camel (Camelus Dromedarius)     

Al-Eknah  M.M.  Homeida  A.M.  Dafalla  E.A.  Galil  A.K.A.  Al-Tahir  A.Y. 《Veterinary research communications》1997,21(1):45-50

Al-Eknah, M.M., Homeida, A.M., Dafalla, E.A., Galil, A.K.A. and Al-Tahir, A.Y., 1997. Uterine activity after induction of hypocalcaemia in the ovariectomized camel (Camelus dromedarius). Veterinary Research Communications, 21 (1), 45-50.Bilateral ovariectomy was performed in three parous, non-pregnant camels. Intrauterine and intra-abdominal pressure changes were recorded using balloon-tipped catheters. Uterine contractions were induced and maintained in the ovariectomized camels by daily intramuscular injections of 5 mg oestradiol benzoate throughout the experimental period. The frequency of uterine contractions varied from 6 to 9 per minute, whereas the amplitude varied from 2 to 3 kPa in all the animals. Inducing hypocalcaemia to a level of 0.5 mmol/L by Na2EDTA reduced the amplitude of the contractions to below 1 kPa (p<0.001). The frequency of the contractions was not affected.  相似文献   

A review of oestrus suppression techniques in mares     

J. R. Crabtree 《Equine Veterinary Education》2022,34(3):141-151

Many fillies and mares are accused of behaving badly by their owners or trainers, and their reproductive hormones and ovaries are commonly blamed for this. Overt oestrus behaviour, however natural, is undesirable when a horse is being ridden, trained and competed. More subtle behaviours may be attributed to the reproductive cycle and presented as the cause of poor performance in the elite competition mare. Sometimes behaviours are complex, perhaps at odds with what one expects for normal reproductive behaviour and in many cases may not be associated with the reproductive cycle at all, rather associated with pain of musculoskeletal or soft tissue origin. The situation represents a diagnostic dilemma to positively associate undesirable behaviours with the ovarian hormones or ovarian pain, before an attempt can be made to modify the behaviour through the suppression of oestrus. This article will review the reasons for presentation, the behavioural patterns of the reproductive cycle and the diagnosis of reproductive behavioural problems. The range of techniques for oestrus suppression will be reviewed along with their clinical application and any known current regulatory issues associated with their use.  相似文献   

Predictive modes of action of pesticides in uterine adenocarcinoma development in rats     

Midori Yoshida  Kaoru Inoue  Miwa Takahashi 《Journal of toxicologic pathology》2015,28(4):207-216

Endometrial adenocarcinoma in the uterine corpus is a malignant cancer that occurs in menopausal women and aged rodents. Because of the similarities in pathogenesis and morphology of endometrial adenocarcinoma in rodents and humans, prediction of the modes of action (MOA) in uterine carcinogenesis is important for extrapolation of rodent data to humans. Three MOAs have been accepted as major pathways for uterine carcinogenesis in rodents: 1) estrogenic activity, 2) increased serum 17beta-estradiiol (E2) to progesterone (P4) ratio and 3) modulation of estrogen metabolism to produce 4-hydroxyestradiol via P450 induction. Inhibition of estrogen excretion and increased aromatase in situ in the tumor are also a potential pathway. Here, chemicals showing uterine carcinogenicity were chosen from approximately 300 pesticides evaluated in Japan within the past decade, and their mechanisms were predicted using parameters from mechanistic and toxicity studies. Seven pesticides increased uterine tumor formation in rats, and the pathways of 4 pesticides could be predicted based on various mechanistic studies. The MOAs of cyenopyrafen and benthiavalicarb-isopropyl were predicted to be modulation of estrogen metabolism, while those of pyriminobac-methyl and spirodiclofen were predicted to be increased E2 to P4 ratio. The driven pathways of metazosulfuron and isopyrazam could not be predicted using several mechanistic studies. No mechanistic studies have been reported for sedaxane, which has a chemical structure and toxicological profile similar to isopyrazam. Our results indicated that appropriate mechanistic studies are useful for mechanism prediction in risk assessment. From this analysis, a flowchart showing a decision tree for predictive MOAs in uterine carcinogenesis was proposed.  相似文献