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Phenolic acids are major components of cell walls in wheat and have important implications on human health as antioxidants with anti-tumor activity. Our objectives were to identify phenolic acid genes in wheat by single nucleotide polymorphisms (SNPs) detected within the coding sequences of candidate genes, and to identify chromosomal regions associated with single phenolic acids and total soluble phenolic compounds. A set of candidate genes involved in the biosynthesis of hydroxycinnamic acid derivatives were identified by comparative genomics. SNPs found in the coding sequences of six genes (PAL1, PAL2, C4H, C3H, COMT1 and COMT2) were used to determine their chromosomal location and accurate map position on two reference consensus linkage maps. The genome-wide association study (GWAS), based on genotyping a tetraploid wheat collection with 81,587 gene-associated SNPs, detected 22 quantitative trait loci (QTL) distributed on almost all durum wheat chromosomes. Two QTL for p-coumaric acid were coincident with the phenylalanine ammonia-lyase (PAL2) and p-coumarate 3-hydroxylase (C3H) genes on chromosome arms 2AL and 1AL, respectively. The availability of candidate gene-based markers can allow elucidating the mechanism of phenolic acids accumulation in wheat kernels and exploiting the genetic variability of phenolic acids content for the nutritional improvement of wheat end-products. 相似文献
3.
施用锌肥和遮阴互作对花生生长发育、抗病性及产量的影响 总被引:1,自引:0,他引:1
采用大田裂区试验,研究了施用锌肥和不同遮阴程度互作对花生生长发育、抗病性及产量的影响。结果表明,与不施锌肥相比,施用锌肥能提高花生不同部位锌含量、增加叶片SPAD值,提高花生叶片中可溶性糖、蛋白质和生长素含量,减少花生病害的发生,平均降低7.1个百分点,花生产量平均增加19.4%。相同施锌水平下,随着遮阴程度的增加,花生不同部位锌的含量和不同生育期叶片SPAD值以及花生叶片中可溶性糖、蛋白质和生长素含量呈增加趋势,花生的发病率比不遮阴对照增加4.8、10.2个百分点,花生产量平均降低16.5%、10.0%。在30%、70%的遮阴条件下,施用锌肥的花生产量比不施锌的分别提高21.1%、25.0%。本试验条件下,施用七水硫酸锌30 kg/hm2,使花生具有较强的抗低温寡照能力及抗病性能,增产显著,可在花生产区推广应用。 相似文献
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中国的甘蓝型油莱高芥酸品种芥酸含量比外国的高芥酸品种高6~10%。本研究用两个中国高芥酸品种与一个加拿大和一个日本高芥酸品种杂交,其Fl与低芥酸品种测交。F2代和测交后代的分离结果表明,这些材料中的芥酸基因是等位的,也无明显的证据表明它们是复等位基因,因而很可能是同一的。这两类品种芥酸含量的差异可能与遗传背景或修饰基因有关。 相似文献
7.
Root length of Holcus lanatus L. declined rapidly with increasing lead and zinc concentrations in nutrient solution. In all used Pb and Zn concentrations root growth of genotypes coming from a Pb-Zn mine area, was greater than that of the control, suggesting that these genotypes evolved tolerance to Pb and Zn.
Negative correlation was also observed between chlorophyll content and increased heavy metal concentrations. The greater chlorophyll content found in tolerant genotypes, in different Pb and Zn concentrations, in comparison with the control, could be served to distinguish tolerant and non-tolerant genotypes. 相似文献
Negative correlation was also observed between chlorophyll content and increased heavy metal concentrations. The greater chlorophyll content found in tolerant genotypes, in different Pb and Zn concentrations, in comparison with the control, could be served to distinguish tolerant and non-tolerant genotypes. 相似文献
8.
日粮锌、硒水平对肉鸡肠道黏膜屏障结构的影响 总被引:3,自引:0,他引:3
为了探讨微量元素锌和硒相互作用对肉鸡肠道黏膜屏障结构的影响,将24只1日龄AA肉鸡随机分3组,分别饲喂添加有高锌高硒(锌1000mg/kg、硒5mg/kg)、低锌低硒(锌34mg/kg、硒0.08mg/kg)或常锌常硒(锌50mg/kg、硒0.15mg/kg)的日粮45d后,观察肠黏膜上皮细胞、上皮内淋巴细胞和盲肠扁桃体的形态结构变化。结果表明:高锌高硒或低锌低硒组肉鸡的肠黏膜结构有明显的损伤,表现为肠黏膜上皮细胞萎缩,绒毛长度下降,上皮内淋巴细胞数量减少;盲肠扁桃体的弥散淋巴组织和淋巴小结中,淋巴细胞数量减少,细胞出现肿胀,有的核消失,结缔组织增生,淋巴小结萎缩。尤其是高锌高硒组的损伤最为严重。而常锌常硒组肉鸡肠黏膜和盲肠扁桃体的形态结构正常。结论:日粮中按锌50mg/kg、硒0.15mg/kg的比例添加,对于维持肠道黏膜的正常屏障结构是合适的。过高或过低的锌和硒对小肠黏膜有毒性作用,破坏其屏障功能;而且高锌和高硒可相互促进以增强其毒性作用。 相似文献
9.
Z. K. Gerechter-Amitai Adriana Grama C. H. Van Silfhout Frida Kleitman 《European journal of plant pathology / European Foundation for Plant Pathology》1989,95(2):79-83
A comparison was made between the genes in 29 new selections of wild emmer wheat resistant to yellow rust over wide geographic areas and the previously extensively studied selectionTriticum dicoccoides G-25. In 23 selections the resistance may be conferred by 1 dominant gene; these include 11 selections in which the gene is different from the dominant gene in sel. G-25 and two others in which the genes were closely linked or allelic to the gene in G-25, differing from sel. G-25 by race-specificity. Two dominant genes different from the gene in sel. G-25, seem to be present in one selection. In five selections the resistance may be conferred by one or two recessive genes, including three instances in which the recessive gene was associated with a dominat gene. Our findings show that at least 19 out of the 29 selections studied possess genes which are different from the gene inT. dicoccoides sel. G-25.Samenvatting In dit onderzoek werden 29 nieuwe resistente wilde-emmer selecties (Triticum dicoccoides) gekruist met de reeds uitvoerig bestudeerde resistente selectie G-25, om na te gaan of de resistentie van de nieuwe selecties wordt veroorzaakt door genen op dezelfde locus als het dominante gen in sel. G-25 of dat er andere loci bij zijn betrokken. De ouders, de F1-en F2-populaties van een bepaalade selectie werden in het kiemplantstadium getoetst met één Israëlisch gele-roest isolaat van fysio 2E0 of van fysio 2E18. In de uitsplitsende F2-populaties werden de niet-sporulerende planten als resistent beschouwd en de sporulerende als vatbaar.In de F2-populaties van 12 herkomsten werden geen vatbare planten gevonden, hetgeen er op duidt dat de resistentie wordt veroorzaakt door een gen op dezelfde locus als het gen in G-25 of door een gen dat neuw gekoppeld is aan het gen in G-25. Voor twee van deze herkomsten kan op basis van een fysio-specifieke interactie worden vastgesteld dat de resistentie berust op allelen die verschillen van het allel in sel. G-25. In 11 herkomsten werd een uitsplitsing voor twee dominante gene gevonden (RS=151), waarbij het tweede dominante gen uit de getoetste nieuwe selectie afkomstig is. De aanwezigheid van twee dominante genen verschillend van het gen in sel. G-25 werd gevonden in één herkomst (631). In de overige vijf selecties bleek de resistentie te worden veroorzaakt door één of twee recessieve genen waarnaast in drie gevallen ook nog een dominant gen werd gevonden.De resultaten tonen aan dat tenminste 19 van de 29 bestudeerde selecties resistentiegenen bezitten die verschillen van het gen inT. dicoccoides sel. G-25. Slechts in twee van deze selecties kan het gen allel zijn met het gen in sel. G-25. 相似文献
10.
Potato diseases caused by soft rot erwinias: an overview of pathogenesis 总被引:17,自引:0,他引:17
M. C. M. Pérombelon† 《Plant pathology》2002,51(1):1-12
Three soft rot erwinias, Erwinia carotovora ssp. carotovora , E. carotovora ssp. atroseptica and E. chrysanthemi are associated with potatoes causing tuber soft rot and blackleg (stem rot). Latent infection of tubers and stems is widespread. As opportunistic pathogens, the bacteria tend to cause disease when potato resistance is impaired. Pathogenesis or disease development in potato tubers and stems is discussed in terms of the interaction between pathogen, host and environment, microbial competition and recent findings on the molecular basis of pathogenicity. Emphasis is placed on the role of free water and anaerobiosis in weakening tuber resistance and in providing nutrient for erwinias to multiply. Blackleg symptoms are expressed when erwinias predominate in rotting mother tubers, invade the stems and multiply in xylem vessels under favourable weather conditions. Soft rot erwinias tend to out-compete other bacteria in tuber rots because of their ability to produce larger quantities of a wider range of cell wall-degrading enzymes. However, despite extensive studies on their induction, regulation and secretion, little is known about the precise role of the different enzymes in pathogenesis. The putative role of quorum-sensing regulation of these enzymes in disease development is evaluated. The role certain pathogenicity-related characters, including motility, adhesion, siderophores, detoxifying systems and the hrp gene complex, common to most bacteria including symbionts and saprophytes, could play in latent and active infections is also discussed. 相似文献