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Summary Spores of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus clarum obtained from sweet potatoes grown in soil inoculated with this fungus and with an enrichment culture of Acetobacter diazotrophicus contained A. diazotrophicus and several other bacteria, including a diazotrophic Klebsiella sp. Inoculation of micropropagated sweet potatoes with G. clarum and A. diazotrophicus enhanced spore formation in soil compared to VAM inoculation alone. Plants inoculated with VAM spores containing the bacteria showed additional increases in the number of spores formed within roots. A. diazotrophicus infected aerial plant parts only when inoculated together with VAM or when present within VAM spores. Micropropagated sugarcane seedlings inoculated with the same VAM spores containing the diazotrophs also contained much higher numbers of A. diazotrophicus in aerial parts than seedlings inoculated in vitro with the bacteria alone. When grown in non-sterile soil, the sugarcane seedlings again showed the greatest infection of aerial parts after inoculation with VAM spores containing the diazotrophs. This treatment also increased VAM colonization and the numbers of spores formed within roots. Similar effects were observed in sweet sorghum except that the aerial plant parts were not infected by A. diazotrophicus. 相似文献
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山西老陈醋优势醋酸菌的分离鉴定 总被引：11，自引：0，他引：11
试验以山西老陈醋醋厂发酵正常的醋醅为试验样品,对其中的醋酸菌采用了变色圈法进行分离,再经过初筛、复筛,根据产酸量高低得到了5株优势醋酸菌(AbT,Af10,Af5,Au13,Af20)。通过对这5株优势菌株的个体形态、培养特征、生理生化特性的研究,将其鉴定为醋酸杆菌属中的巴氏醋杆菌(Ab7)、醋化醋杆菌(Af10)、液化醋杆菌(Af5)、汉氏醋杆菌(Au13)和恶臭醋杆菌(Af20)。通过本试验的研究可为山西老陈醋的进一步发展奠定菌种基础。 相似文献
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M. A. Paula S. Urquiaga J. O. Siqueira J. Döbereiner 《Biology and Fertility of Soils》1992,14(1):61-66
Summary Sweet potatoes were micropropagated and then transplanted from axnic conditions to fumigated soil in pots in the greenhouse. Spores of Glomus clarum were obtained from Brachiaria decumbens or from sweet potatoes grown in soil infected with this fungus and with an enrichment culture of Acetobacter diazotrophicus. Three experiments were carried out to measure the beneficial effects of vesicular-arbuscular mycorrhizal (VAM) fungi-diazotroph interactions on growth, nutrition, and infection of sweet potato by A. diazotrophicus and other diazotrophs obtained from sweet potato roots. In two of these experiments the soils had been mixed with 15N-containing organic matter. The greatest effects of mycorrhizal inoculation were observed with co-inoculation of A. diazotrophicus and/or mixed cultures of diazotrophs containing A. diazotrophicus and Klebsiella sp. The tuber production was dependent on mycorrhization, and total N and P accumulation were increased when diazotrophs and G. clarum were applied together with VAM fungal spores. A. diazotrophicus infected aerial plant parts only when inoculated together with VAM fungi or when present within G. clarum spores. More pronounced effects on root colonization and intraradical sporulation of G. clarum were observed when A. diazotrophicus was co-inoculated. In non-fumigated soil, dual inoculation effects, however, were of lower magnitude. 15N analysis of the aerial parts and roots and tubers at the early growth stage (70 days) showed no statistical differences between treatments except for the VAM+Klebsiella sp. treatment. This indicates that the effects of A. diazotrophicus and other diazotrophs on sweet potato growth were caused by enhanced mycorrhization and, consequently, a more efficient assimilation of nutrients from the soil than by N2 fixation. The possible interactions between these effects are discussed. 相似文献
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开菲尔粒中醋酸菌的分离鉴定 总被引：1，自引：0，他引：1
从开菲尔粒中分离出2株醋酸菌FJAT-13764和FJAT-13780.利用形态学观察、生理生化和16S rDNA方法对菌株进行分类鉴定.菌株FJAT-13764和FJAT-13780的菌体形态均为圆端直杆菌,单个或成对,无芽孢,其生理生化特征与醋杆菌属Acetobacter基本一致.经16S rDNA基因序列同源性比较和系统发育分析,鉴定菌株FJAT-13764为Acetobacter orientalis(东方醋酸菌),菌株FJAT-13780为Acetobacter syzygii. 相似文献
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高产醋酸菌的筛选及其形态生化特征研究 总被引：14，自引：1，他引：14
张忠明 《甘肃农业大学学报》2006,41(1):83-86
从天然发酵醋醅中分离得到了32株醋酸菌,经初筛、复筛产酸试验,筛选出了J4和J82株醋酸菌.采用紫外线诱变处理始发J4菌株,经分离筛选获得产醋酸能力高的醋酸菌J4-1和J4-4,产酸量分别为85.44 g/L和87.56 g/L,J4-4产酸量比菌株J4(84.19 g/L)提高了4%,并对J4-4菌株的形态生化特征进行了研究. 相似文献
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通过紫外线和He-Ne激光诱变醋酸菌,选育出1株乙醛脱氢酶高产菌株Z07-J01,该正突变株24 h发酵酶活为1 008.00 U/g湿菌体,比出发株提高了276%,且酶活在10代内稳定。该突变株所产乙醛脱氢酶的最适作用pH为7,最适作用温度为50℃;在25℃保温30 min,该酶酶活不受影响;在65℃保温30 min,该酶完全失活;2 mmol/L Cu2 能使该酶完全失活,2 mmol/L Mn2 则对该酶有较强的激活作用。 相似文献
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R. Muthukumarasamy G. Revathi C. Lakshminarasimhan 《Biology and Fertility of Soils》1999,29(2):157-164
Observations of newer diazotrophs like Acetobacter diazotrophicus and Herbaspirillum spp. in Indian sugarcane varieties is reported here for the first time. A. diazotrophicus was isolated from sugarcane plants where N fertilisation was nil, and numbers in the Indian cultivars were estimated to be
up to 106–107 colony forming units g1 fresh weight. N fertilisation was the limiting factor for the isolation of A. diazotrophicus, as evidenced by selective growth patterns in different concentrations of N sources, but Herbaspirillum spp. were found to occur both in N fertilised and unfertilised samples. The fact that N is not the limiting factor for Herbaspirillum spp. was evidenced in a similar study of its growth pattern in different N sources.
Received: 14 May 1998 相似文献
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