中国李RAPD的优化反应体系及其在品种鉴定中的应用   总被引：20，自引：0，他引：20  

乔玉山  章镇  房经贵  郭洪 《果树学报》2003,20(6):445-449

以5'-GACCGCTTGT-3'为随机引物,以奉化李(Prunussalicinacv.Fenghuali)为试材,对中国李RAPD反应体系进行了优化研究,结果表明:25μL反应体系中,TaqDNA聚合酶、Mg2+、引物、模板DNA和dNTPs5种主要成分的适宜浓度或用量分别是:1.0U、2.5mmol/L、20ng、40ng和0.2mmol/L。利用该优化反应体系,用10个随机引物,构建了5个中国李品种的RAPD指纹图谱;并以共有谱带率对这些品种遗传关系进行了分析,5个中国李品种的共有谱带率平均为33.2%,变异幅度为20.1%～44.9%。  相似文献   

指纹技术识别泥沙来源:不确定性研究进展   总被引：2，自引：0，他引：2  

周慧平  周宏伟  陈玉东 《土壤学报》2019,56(6):1279-1289

泥沙指纹技术是近年来研究流域泥沙来源与动态变化的一项重要技术方法,其主要局限性在于诸多的前提假设与实践条件的差异带入了一系列不确定性。泥沙从源到汇的迁移过程受自然环境和人为干扰的影响,其源地特征、迁移路径以及泥沙性质的潜在变化等均是指纹技术应用的不确定性因素,而采样方案设计、指纹选择方法、混合模型应用的一些主观因素进一步增加了不确定性的来源。不确定性分析是泥沙指纹技术的重要组成部分,是评估泥沙来源指纹识别结果可靠性的主要依据。本文综述了泥沙指纹技术的诸多不确定性问题以及主要应对方法的研究进展,总结了当前不确定性定量评估的研究现状并提出了未来的研究展望。  相似文献   

直接扩增甜瓜小卫星DNA指纹图谱   总被引：4，自引：0，他引：4  

王掌军  王建设  刘玲  刘生祥  陈素生  许勇  王永健 《华北农学报》2006,21(3):77-80

以小卫星DNA YNZ22核心序列为引物,甜瓜DNA为模板,在甜瓜上研究了直接扩增小卫星DNA(DAMD)的优化反应体系,结果表明:在20μL的反应体系中,5种主要成分Taq DNA聚合酶、Mg2 、引物、模板DNA和dNTPs的最适浓度分别为1U,2.5 mmol/L,0.5 mmol/L,30 ng,5 mmol/L。在优化的DAMD-PCR反应体系下,利用该引物在28个甜瓜品种上构建了直接扩增小卫星DNA(DAMD)的指纹图谱,分析结果表明,该引物在28个甜瓜品种上共扩增出13位点,其中9位点具有多态性,多态性条带比率为69%,可一次性从28个甜瓜品种中鉴别出其中的21个,鉴别率高达75%。  相似文献   

引进观赏芍药新种质的SSR指纹图谱构建   总被引：2，自引：0，他引：2  

张建军  季丽静  刘仲赫  周明洁  王雅丽  于晓南 《东北林业大学学报》2015,(3):70-74

采用SSR标记构建从欧美等地区引进、收集的61份观赏芍药新种质的指纹图谱。根据引物的多态性信息含量和Shannon遗传多样性指数大小,从15对候选引物中筛选出10对多态性高且稳定性好的引物作为核心引物,首次构建了61个观赏芍药新种质指纹图谱,并编制了指纹代码。结果表明:被试种质资源的倍型丰富,有一条带、两条带,甚至出现了三条带、四条带;所绘制的图谱能够将所有品种(种)完全区分开。  相似文献   

中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:RAPD指纹标记   总被引：20，自引：3，他引：20  

李思发  邹曙明 《水产学报》1999,(4)

用 4 8个具有丰富多态性的 10碱基随机引物对辽河、黄河、长江、瓯江、珠江和南流江的中华绒螯蟹和日本绒螯蟹的 6个群体进行RAPD分析。结果发现 :(1)两个引物具有群体特异性带 ,其中Z2扩增的 880bp片段为珠江蟹和南流江蟹群体中所特有 ,而 70 0bp片段为长江蟹、辽河蟹、黄河蟹和瓯江蟹所特有 ,可作为区别日本绒螯蟹和中华绒螯蟹的分子遗传标记 ;(2 )Opp17扩增的 94 7bp片段的出现频率 ,在长江、黄河和辽河群体显著地从南到北递减 ,长江蟹 87.50 %、黄河蟹4 1.66%、辽河蟹 10 .83%。这一遗传渐变的度量可作为区别这三个水系的中华绒螯蟹种群的判据 ;(3) 6水系群体内的平均遗传相似度依次为 :辽河蟹 0 .90 8>南流江蟹 0 .897>瓯江蟹和珠江蟹 0 .895>黄河蟹 0 .890 >长江蟹 0 .850 ;6群体间遗传距离及其UPGMA、NJ聚类分析进一步表明 ,南流江蟹、珠江蟹同长江蟹、辽河蟹及黄河蟹在基因组之间存在明显的歧化。  相似文献   

中国大陆沿海六水系绒螯蟹_中华绒_省略__群体亲缘关系_RAPD指纹标记     

李思发 《水产学报》1999,23(4)

用 4 8个具有丰富多态性的 10碱基随机引物对辽河、黄河、长江、瓯江、珠江和南流江的中华绒螯蟹和日本绒螯蟹的 6个群体进行RAPD分析。结果发现 :(1)两个引物具有群体特异性带 ,其中Z2扩增的 880bp片段为珠江蟹和南流江蟹群体中所特有 ,而 70 0bp片段为长江蟹、辽河蟹、黄河蟹和瓯江蟹所特有 ,可作为区别日本绒螯蟹和中华绒螯蟹的分子遗传标记 ;(2 )Opp17扩增的 94 7bp片段的出现频率 ,在长江、黄河和辽河群体显著地从南到北递减 ,长江蟹 87.50 %、黄河蟹4 1.66%、辽河蟹 10 .83%。这一遗传渐变的度量可作为区别这三个水系的中华绒螯蟹种群的判据 ;(3) 6水系群体内的平均遗传相似度依次为 :辽河蟹 0 .90 8>南流江蟹 0 .897>瓯江蟹和珠江蟹 0 .895>黄河蟹 0 .890 >长江蟹 0 .850 ;6群体间遗传距离及其UPGMA、NJ聚类分析进一步表明 ,南流江蟹、珠江蟹同长江蟹、辽河蟹及黄河蟹在基因组之间存在明显的歧化。 更多还原  相似文献   

Genotyping of Mycobacterium bovis by geographic location within Mexico   总被引：1，自引：0，他引：1  

Milián-Suazo F  Banda-Ruíz V  Ramírez-Casillas C  Arriaga-Díaz C 《Preventive veterinary medicine》2002,55(4):e129-264

The spacer oligonucleotide typing (spoligotyping) method was used to differentiate 62 Mycobacterium bovis isolates obtained from tissues with macroscopic lesions typical of tuberculosis in dairy cattle from different regions of Mexico. Our purpose was to see if a strain from one region was genetically different from those of other regions (with the long-term aim of doing molecular trace back of isolates obtained in the laboratory). Results from the genetic analysis indicate that M. bovis isolates cannot be grouped by geographic location due to a wide range of genetic types involved in dairy cattle infections. Isolates even from the same herd showed different spoligotypes but some isolates from different region had similar genetic patterns. Genetic typing without epidemiologic information does not seem to be a plausible method to trace back animals to source of origin to detect and eliminate sources of infection.  相似文献   

番茄品种SSR标记鉴定技术研究   总被引：1，自引：0，他引：1  

赵海艳  吴明生  宋歌  牛茜  田慧芳 《中国蔬菜》2015,1(8):22-27

以16个番茄品种为材料,从200对番茄SSR引物中筛选出20对适合用于番茄品种鉴定的引物,用该20对引物对66个番茄品种进行分析,共检测出52个等位基因变异,等位基因变异范围为2~5个,平均每个位点产生2.6个等位基因变异。通过遗传聚类方法对引物组合的品种鉴别能力进行分析,结果显示仅用9对引物构成的引物组合就可以对66个番茄品种进行有效鉴别,该9对引物组合构建的SSR-DNA指纹图谱可作为品种的遗传标签用于品种真伪鉴定。  相似文献   

Database derived microsatellite markers (SSRs) for cultivar differentiation in <Emphasis Type="Italic">Brassica oleracea</Emphasis>     

S. Louarn  A. M. Torp  I. B. Holme  S. B. Andersen  B. D. Jensen 《Genetic Resources and Crop Evolution》2007,54(8):1717-1725

Fifty-nine Brassica oleracea cultivars, belonging to five botanical varieties, were evaluated for microsatellite (SSR) polymorphisms using 11 database sequence derived primer pairs. The cultivars represented 12 broccoli (Brassica oleracea var. italica), ten Brussels sprouts (B. o. var. gemmifera), 21 cabbage (B. o. var. capitata, including the groups white and red cabbage), six savoy cabbage (B. o. var. sabauda), and ten cauliflower (B. o. var. botrytis) cultivars from 13 seed suppliers. The 11 primer pairs amplified in total 47 fragments, and differentiated 51 of the cultivars, whereas the remaining eight cultivars were differentiated from the rest in four inseparable pairs. All SSR markers, except one, produced a polymorphic information content (PIC value) of 0.5 or above. The average diversity for all markers within the tested material was 0.64. There was no major difference in the diversity within botanical varieties and groups. The cluster analysis and the resulting dendrogram showed that the cultivars tended to group within these taxonomic units. The present study substantiates the use of microsatellite markers as a powerful tool for cultivar differentiation and identification in vegetable brassicas.  相似文献   

Multi-parameter fingerprinting of sediment deposition in a small gullied catchment in SE Australia   总被引：4，自引：0，他引：4  

A. K. Krause  S. W. Franks  J. D. Kalma  R. J. Loughran  J. S. Rowan 《CATENA》2003,53(4):327-348

The determination of relative contributions of potential sediment sources is an important step in the development of management strategies to combat soil erosion. In a 1.2 km² gullied catchment in southeastern New South Wales, multi-parameter fingerprinting of sediment deposited in successive downstream pools has identified gully walls as the dominant sediment source when the grazed pasture surface was the only other potential source. The median fractional contributions remained relatively steady in the successive downstream pools, with the gully walls responsible for between 90% and 98% of the pool sediment. This result was achieved despite the ratio of the source areas varying considerably between successive nested subareas. Reliability bounds on the predictions, accounting for limited sampling of sources, were well constrained and varied between 5.4% and 13.8%. Downstream of an unsealed road crossing, sediment from the road source dominated the pool sediments such that contributions from the pasture surface and gully sources could not be determined.  相似文献