脑活素治疗新生大鼠缺血缺氧性脑病机理的研究   总被引：1，自引：0，他引：1  

张瑞莉  高雪丽  郑世民 《黑龙江畜牧兽医》2002,(6):1-3

7日龄Wister大鼠结扎左侧颈总动脉后吸入氧氮混合气体（8％O2和92％N2）2h,制成缺血缺氧性脑病动物模型,测定大鼠脑组织中丙二醛（MDA）的含量和超氧化物歧化酶（SOD）的活性。结果显示,缺血缺氧后6h,缺血缺氧组MDA含量显著升高,24h达到高峰,以后逐渐下降,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组MDA含量于缺血缺氧后6h已经显著下降,72h与对照组比较无统计学差异。缺血缺氧组SOD活力值在缺血缺氧后6h已经下降,24h降到最低点,以后逐渐回升,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组SOD活力值在缺血缺氧后6h明显提高,于缺血缺氧后96h与对照组比较无统计学差异。结果提示,缺血缺氧引起新生大鼠脑组织的氧化-抗氧化系统失衡,氧自由基大量产生,参与了新生大鼠缺血缺氧性脑病脑损伤过程;脑活素能够抑制氧自由基的生成,提高SOD的活力值,对缺血缺氧性脑病具有神经保护作用。  相似文献   

Effects of nitric oxide on hepatic encephalopathy in cirrhotic rats induced by LPS     

LIU Jin-chun  LI Yan-jiao  HAN De-wu  NIU Chun-hong  XU Rui-ling  ZHAO Yuan-chang 《园艺学报》2002,18(10):1248-1250

AIM: To investigate the effects of nitric oxide (NO) on hepatic encephalopathy in cirrhotic rats induced by LPS. METHODS: The cirrhotic model of rats was established by complex pathogeny. Since the end of the 8 th week, the rats were intragastrically-infused with 0.9% salt, L-arginine(L-arg) and LNNA respectively for 2 weeks.The hepatic encephalopathy in cirrhotic rats were induced by 3 mg/kg LPS (ip) 4 hours before the rats were sacrificed. RESULTS: The normal behaviors and electroencephalograph were appeared in L-arg group. LNNA group showed hepatic encephalopathy. The content of NO₂^-/NO₃^- of brain tissue was markedly higher in L-arg group than LNNA group(P<0.05), but the content of histamine in brain tissue was lower in L-arg group than LNNA group(P<0.05). There was a negative correlation between the content of histamine in brain tissue and the content of NO₂^-/NO₃^- of brain tissue. CONCLUSION: NO can prevent hepatic encephalopathy in cirrhotic rats induced by LPS.  相似文献   

Pharmacokinetics of the anticonvulsant levetiracetam in neonatal foals          下载免费PDF全文

K. D. MacDonald  K. A. Hart  J. L. Davis  L. J. Berghaus  S. Giguère 《Equine veterinary journal》2018,50(4):532-536

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Study on the relationship between melatonin and hepatic encephalopathy     

WU Wan-chun  ZHANG Guo-zheng  WANG Yu-xiang  HE Chi-yi 《园艺学报》2000,16(9):845-847

AIM:To explore the relationship between change of serum melatonin (MT) and pathogenesis of hepatic encephalopathy (HE). METHODS: Changes of MT level in sera of cirrhosic patients with HE and without HE were determined by ELISA, normal serum served as control. The change of serum MT level in exacerbation and remission in HE was also determined.RESULTS:MT level in patients with HE was higher than that withour HE (P＜0.01). MT levels of both groups were higher than that of normal group (P＜0.01). They were (308.53±59.07) ng/L, (139.85±34.59)ng/L,(77.73±28.41)ng/L, respectively. Serum MT level in exacerbation was higher than that in remission (P＜0.01), they were (301.52±66.42)ng/L and (147.81±23.31) ng/L, respectively. CONCLUSION: The elevation of MT content in sera may be closely related to the onset of hepatic coma.  相似文献   

Effects of noninvasive delayed limb ischemic preconditioning on prognosis of myocardial infarction     

SUN Kai  CHEN Wen-hua  ZHANG Ying  LI Yan  AN Meng-yao  PAN Ying-ying  WU Yan-na  KANG Yi  GAO Wei-zhen  LOU Jian-shi 《园艺学报》2017,33(1):116-122

AIM: To study the effects of noninvasive delayed limb ischemia preconditioning (NDLIP) on animal cardiac function, myocardial morphology and myocardial apoptosis after myocardial infarction (MI). METHODS: Healthy SD male rats[n=45, weighing (250±10) g] were randomly divided into 3 groups:MI group:the animal model of MI was established by surgical ligation of left anterior descending artery (LAD) after 2 weeks; NDLIP group:after the success of the MI animal model, NDLIP was carried out every other day until the 4th, 6th and 8th weeks; sham group:as the negative control group, the animals were taken heart LAD threading but no ligation. All rats were fed conventionally. At the end of the 4th, 6th and 8th weeks, all rats were made ventricular intubation, and then the hemodynamic parameters were recorded. The blood samples were withdrawn from the abdominal aorta and the serum was separated via centrifugation. The serum contents of Bcl-2 and Bax were measured by ELISA. Left ventricular anterior wall was homogenized. The mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ and Ⅳ in the myocardial tissues were detected by ELISA. RESULTS: At the end of the 4th, 6th and 8th weeks, compared with MI group, left ventricular systolic pressure in NDLIP group was significantly increased, while left ventricular end-diastolic pressure in NDLIP group was significantly decreased (both P<0.05). Mitochondrial respiratory chain complexesⅠ, Ⅱ, Ⅲ and Ⅳ in NDLIP group were significantly increased (P<0.05). The serum level of Bcl-2 in NDLIP group was significantly increased and Bax level was reduced remarkably (both P<0.01). CONCLUSION: NDLIP improves the hemodynamic indexes, promotes the mitochondrial respiratory function and inhibits cell apoptosis, thus improving the prognosis of MI.  相似文献   

Ellagic acid attenuates hypoxic-ischemic brain injury by alleviating autophagy     

CAI Chen-chen  YE Li-xia  ZHU Jiang-hu  BAI Jun-jie  ZENG Shan-shan  CHEN Shang-qin  LIN Zhen-lang 《园艺学报》2019,35(2):311-319

AIM:To investigate whether ellagic acid (EA) attenuates hypoxic-ischemic encephalopathy (HIE) by down-regulating autophagy. METHODS:In vivo, Sprague-Dawley rats (n=17) were randomly divided into 3 groups:5 rats for sham group, 6 rats for HIE group and 6 rats for HIE+EA pretreatment group. The rats in HIE+EA pretreatment group were treated with EA (10 mg/kg, 10 mL/kg, suspended in corn oil, ig). After 24 h of operation, the rats from each group were sacrificed and their brains were collected. TTC staining and HE staining were used to define the infarct areas and brain structure. The autophagy-related proteins beclin-1, P62, LC3-Ⅱ/-I and Atg5 in the cortex in each group were compared by Western blot. In vitro, PC12 cells were divided into 3 groups:control group, CoCl₂ group and CoCl₂+EA pretreatment group. CoCl₂ at 800 μmol/L was added to the PC12 cells to induce an anoxic environment. The PC12 cells were pretreated with EA at 8 μmol/L and the cell viability was measured by CCK-8 assay. The production of reactive oxidative species (ROS) in the cells was detected by flow cytometry with DCFH-DA staining. MDC staining and TMRE staining were applied to reflect the extent of autophagy and the state of apoptosis, respectively. The autophagy-related proteins in PC12 cells were also investigated. RESULTS:In HIE group, 7-day-old rats were given the operations and the their large infarct areas in the hemisphere were observed by TTC staining. HE staining displayed the injured hemispheres which contained few neurons, and exhibited edema status and serious structural damage. EA pretreatment decreased the infarct area and alleviated the damage to hemisphere with more visible neurons, compared with HIE group. Compared with sham group, the levels of autophagy-related proteins Atg5, beclin-1 and LC3-Ⅱ/-I in the cortex were increased (P<0.01), and P62 protein expression was decreased (P<0.01) in HIE group. Compared with HIE group, the protein expression of Atg5, beclin-1 and LC3-Ⅱ/-I was decreased (P<0.01) and P62 protein expression was increased in HIE+EA pretreatment group (P<0.01). In vitro, compared with CoCl₂ group, the PC12 cells in CoCl₂+EA pretreatment group showed a lower ROS level. Moreover, the cells in CoCl₂+EA pretreatment group exhibited higher mitochondrial membrane potential than that in CoCl₂ group. MDC staining in CoCl₂ group showed high value of fluorescence and increased number of autophagosomes. EA pretreatment reduced the number of autophagosomes and the extent of autophagy to protect PC12 cells. Furthermore, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I in CoCl₂ group were higher (P<0.01), and the protein expression of P62 was lower (P<0.01) than those in control group. In CoCl₂+EA pretreatment group, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I were decreased (P<0.01) and the protein expression of P62 was increased as compared with CoCl₂ group (P<0.01). CONCLUSION:EA pretreatment attenuates autophagy to protect the neurons against HIE injury.  相似文献   

Protective immunity of sevenband grouper, Epinephelus septemfasciatus Thunberg, against experimental viral nervous necrosis   总被引：9，自引：0，他引：9  

S Tanaka  K Mori  M Arimoto  T Iwamoto  & T Nakai 《Journal of fish diseases》2001,24(1):15-22

This paper describes the protective immune responses of sevenband grouper, Epinephelus septemfasciatus Thunberg, immunized with live piscine nodavirus, the causative agent of viral nervous necrosis (VNN), or the Escherichia coli – expressed recombinant coat protein. Nodavirus-neutralizing antibodies were detected at titres ranging from 1:158 to 1:1257 in serum of sevenband grouper which survived intramuscular injection with the virus, by a cell culture assay system. The virus-neutralizing ability of immune serum was also confirmed by injecting virus previously treated with serum into fish. This indicates establishment of acquired immunity in survivors and thus explains why survivors from natural infection are resistant to recurrence of the disease. Young sevenband grouper were immunized twice by intramuscular injections with the recombinant coat protein. Immunized fish produced neutralizing antibodies at high titres for at least 110 days and showed significantly lower mortalities in virus challenge tests. These results suggest the potential for vaccination against VNN in sevenband grouper, which is susceptible to piscine nodavirus at all life-stages.  相似文献   

Experimental transmission of encephalopathy and retinopathy induced by nodavirus to sea bream, Sparus aurata L., using different infection models   总被引：1，自引：0，他引：1  

R Aranguren  C Tafalla  B Novoa  & A Figueras 《Journal of fish diseases》2002,25(6):317-324

The transmission of viral encephalopathy and retinopathy (VER) was investigated in juvenile sea bream, Sparus aurata L. Two different infection routes [intraperitoneal (i.p.) and intramuscular injection (i.m.)] were tested at two different temperatures (20 and 26 °C) using sea bream of mean weight 0.7, 2 and 4 g, as well as an immersion challenge performed at 26 °C with sea bream of 0.7 g. Successful transmission of the disease was only achieved by i.m. injection. Mortalities of 100% occurred in sea bream of 0.7 g at day 15 post-infection and 47% in sea bream of both 2 and 4 g at day 30 post-infection in all the experimental infections at 26 °C. No mortalities were ever observed with infections at 20 °C. When mortalities were observed, the virus was detected by immunoperoxidase staining in the SSN-1 cell line inoculated with tissues from infected fish. Histological examination of both normal and infected fish showed a vacuolization in the bipolar and granular layers of the retina of the infected sea bream. This is the first experimentally induced transmission of VER in sea bream. Differences were observed at the time of disease onset depending on water temperature, the route of infection and the age of the juvenile fish.  相似文献   

Comparative pathogenicity study of ten different betanodavirus strains in experimentally infected European sea bass,Dicentrarchus labrax (L.)     

N Vendramin  A Toffan  M Mancin  E Cappellozza  V Panzarin  G Bovo  G Cattoli  I Capua  C Terregino 《Journal of fish diseases》2014,37(4):371-383

Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a severe pathological condition caused by RNA viruses belonging to the Nodaviridae family, genus Betanodavirus. The disease, described in more than 50 fish species worldwide, is considered as the most serious viral threat affecting marine farmed species in the Mediterranean region, thus representing one of the bottlenecks for further development of the aquaculture industry. To date, four different genotypes have been identified, namely red‐spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV), tiger puffer nervous necrosis virus and barfin flounder nervous necrosis virus, with the RGNNV genotype appearing as the most widespread in the Mediterranean region, although SJNNV‐type strains and reassortant viruses have also been reported. The existence of these genetically different strains could be the reason for the differences in mortality observed in the field. However, very little experimental data are available on the pathogenicity of these viruses in farmed fish. Therefore, in this study, the pathogenicity of 10 isolates has been assessed with an in vivo trial. The investigation was conducted using the European sea bass, the first target fish species for the disease in the Mediterranean basin. Naive fish were challenged by immersion and clinical signs and mortality were recorded for 68 days; furthermore, samples collected at selected time points were analysed to evaluate the development of the infection. Finally, survivors were weighed to estimate the growth reduction. The statistically supported results obtained in this study demonstrated different pathogenicity patterns, underlined the potential risk represented by different strains in the transmission of the infection to highly susceptible species and highlighted the indirect damage caused by a clinical outbreak of VER/VNN.  相似文献   

Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax,Linnaeus 1758) using non‐lethal sampling methods     

Inês Almeida Ferreira  Janina Z. Costa  Valeria Macchia  Kimberly Dawn Thompson  Teresa Baptista 《Journal of fish diseases》2019,42(8):1097-1105

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