不拘于形，不役于物——欧阳修的园林活动与园林书写     

韦雨涓 《广东园林》2020,42(5):92-96

欧阳修自幼与园林结缘，仕宦四十年，所到之处植花造园，笔耕不辍，留下数座园林景观、二十多篇园亭记及百余首园林诗词，撰写了现存最早的牡丹专著《洛阳牡丹记》，为后人研究园林提供了珍贵的史料。欧阳修的园林活 动和园林书写与其宦海沉浮、文学创作紧密相关。欧阳修热爱但不耽溺于园林，其崇尚自然、开放自适，不拘于形，不役于物的园林观，拓宽了园林审美的视域，启发了后人对“物”的审美思考，为园林理论注入了新鲜血液。欧阳修进一步打开了园林的大门，推动了园林的开放，为园林的繁荣与普及做出贡献。  相似文献   

人为影响下古尔班通古特沙漠小尺度生态环境变化   总被引：1，自引：1，他引：1  

钱亦兵  张立运  雷加强  吴兆宁  张锐 《干旱区资源与环境》2003,17(6):82-88

随着沙漠中油气资源勘探开发、公路建设和重大工程相继实施 ,对生态环境的人为影响日益加深。本文以重大工程实验点为例 ,通过研究对比工程影响区内人工沙垄和自然沙垄的砂质新成土理化性状和植被群落特征 ,指出了沙漠生态环境在人为干扰下发生的一系列变化 ,以及受损生态环境在人们采取积极补救措施的情况下所具有的自然修复能力  相似文献   

纳米微粒材料生物学活性研究进展   总被引：1，自引：0，他引：1  

亓立峰  许梓荣  江霞  胡彩虹 《中国兽药杂志》2004,38(8):25-28

综述了纳米微粒的生物学效应及其免疫调节、抗菌、抗肿瘤等多种生物学活性.  相似文献   

君子兰转化酶基因片段(CMCW1)的克隆和序列分析     

汪琼  王燕  程水源  费永俊  金卫斌 《园艺学报》2004,31(2):256-259

 分析植物酸性转化酶基因的保守区序列，设计一对PCR引物，以君子兰基因组DNA为模板，采用PCR方法扩增出长约500 bp的DNA片段，克隆入pGEM-TEasy载体，测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1，该基因片段长518 bp，不含内含子，编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明，该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。  相似文献   

噻二唑基-3-哒嗪酮类化合物的合成及生物活性   总被引：1，自引：0，他引：1       下载免费PDF全文

曹松  曲玉成  宋恭华  钱旭红  黄青春  卢德力 《农药学学报》2004,6(2):68-71

将取代的二酰基肼环合后,得到中间体2-芳基-5-氯甲基-1,3,4-噻二唑,然后与2-叔丁基-4-氯-5-羟基-3(2H)-哒嗪酮反应,合成了8个未见文献报道的含噻二唑基哒嗪酮类化合物,其化学结构经1H NMR、高分辩质谱和元素分析确认。生物活性测试结果表明,部分化合物对粘虫P.separate W.有较好的抑制生长活性,其中化合物 3b 的EC50值为21 mg/L。  相似文献   

Identification of organic acid-related genes and their expression profiles in two pear (Pyrus pyrifolia) cultivars with difference in predominant acid type at fruit ripening stage     

Xiao-Peng Lu  Yong-Zhong Liu  Gao-Feng Zhou  Qing-Jiang Wei  Hong-Ju Hu  Shu-Ang Peng 《Scientia Horticulturae》2011

‘Yandangxueli’ is a pear cultivar with predominant citric acid in the ripe fruit, different from most of pear cultivars such as ‘Gengtouqing’ in which malic acid is the predominant acid type. It was found that ‘Yandangxueli’ accumulated citric acid for three times against that in ‘Gengtouqing’ at fruit ripening stage. To investigate the mechanism of citric acid accumulation in ‘Yandangxueli’, organic acids content, gene expression and enzyme activity were studied in both cultivars. Five genes, Pp:mtCs, Pp:cyAco, Pp:cyIdh, Pp:mtMdh and Pp:cyMe which encoded citric synthase (CS), cytosolic aconitase (cyACO), NADP-dependent isocitrate dehydrogenase (NADP-IDH), NAD-dependent malate dehydrogenase (NAD-MDH) and NADP-dependent malic enzyme (NADP-ME) respectively, were identified from pear fruit. Their expression profiles and the corresponding enzyme activities were determined throughout fruit development in both cultivars. Results from these enzymes indicated that there were no strict relationship between gene expression, enzyme activity and citric acid accumulation. Expression analysis for two Py:vVAtp genes encoding vacuolar H⁺-ATPase A subunit and one Py:vVpp gene encoding Vacuolar H⁺-pyrophosphatase showed that they were all with up-regulated expression at the later development stage of ‘Yandangxueli’ but with down-regulated expression in ‘Gengtouqing’. Therefore, it is concluded that the different ability in citric acid transportation and storage might be involved in the high citric acid content in ‘Yandangxueli’.  相似文献   

不同供磷水平对柱花草和黑籽雀稗根际生理活性的影响     

余爱  张海达  吴露露  张宇  杨帆  唐树梅  漆智平 《热带作物学报》2011,31(6):7-10

以热带柱花草和黑籽雀稗为材料，通过盆栽试验，研究不同磷水平对其根际酸性磷酸酶、微生物数量及根系活力的影响。结果表明：2种牧草根际酸性磷酸酶(ACP)活性随着磷浓度的增加而降低，在低磷(P0)处理时达最高，柱花草的ACP活性为603.24 mg/(kg·h)，黑籽雀稗为367.21 mg/(kg·h)。当高磷浓度处理时，柱花草根际ACP活性较黑籽雀稗低。各处理中，柱花草的根际微生物总量较黑籽雀稗高，柱花草根际真菌的数量随磷浓度的增加而减少，处理间差异达极显著水平，低磷处理时土壤真菌数量较多，这有利于根瘤菌的形成。2种牧草的根系活力随着施磷浓度的增加而递减。黑籽雀稗根系活力P0处理极显著高于P3和P4处理,显著高于P2处理。黑籽雀稗根系活力受供磷水平的影响大于柱花草。  相似文献   

莰烯醛O-取代肟的合成及其抗肿瘤活性     

黄静  刘辰婧  罗金岳 《林产化学与工业》2020,40(1):53-60

以莰烯醛肟与卤代物为原料经亲核取代反应合成了9个未见报道的莰烯醛O-取代肟类化合物,分别为2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-苄基肟(2a)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-丁基肟(2b)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(4-氯丁基)肟(2c)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(3-溴苄基)肟(2d)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(4-叔丁基苄基)肟(2e)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(4-氯苄基)肟(2f)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(4-氰基苄基)肟(2g)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(2,6-二氯苄基)肟(2h)、2-(3,3-二甲基双环[2.2.1]庚-2-亚基)乙醛O-(邻氟苄基)肟(2i)。利用FT-IR、GC-MS、1H NMR以及13C NMR对产物结构进行了表征。以化合物2a为例,探索了不同工艺条件对产物得率的影响,在甲苯为溶剂,n(莰烯醛肟)∶n(氯化苄)∶n(四丁基溴化铵)为1.0∶1.8∶0.08,反应温度为60℃,反应时间为20 h的最佳工艺条件下,产物的得率为84.1%。通过体外抗肿瘤活性测试,探讨了化合物2a^2i对肝癌细胞HepG2和人乳腺癌细胞MCF7的抑制作用,结果表明:化合物2b对HepG2细胞的抑制作用较好,其半数抑制浓度(IC 50)值为36.3μmol/L;化合物2d、2h、2i对MCF7有一定的抑制作用,其中化合物2h对MCF7的抑制作用较好,其IC50值为19.2μmol/L。  相似文献   

茶树凋落叶浸提液对菘蓝生理生化的化感效应     

沙俊涛  陈青青  缪雨静  屈仁军  唐晓清  房婉萍 《核农学报》2020,34(6):1327-1336

为研究茶树凋落叶浸提液对菘蓝生长与生理生化的化感效应,以模拟自然条件下雨雾淋溶方式,采用不同浓度的茶树凋落叶浸提液(CK:0 mg·mL^-1、T1:6.25 mg·mL^-1、T2:12.5 mg·mL^-1、T3:25 mg·mL^-1 和T4:50 mg·mL^-1)处理菘蓝,测定其生长、抗氧化酶活性及其基因表达量、细胞膜损伤率以及丙二醛(MDA)、过氧化氢(H₂O₂)、渗透调节物质和次生代谢物含量的变化。结果表明,茶树凋落叶浸提液对菘蓝生长表现出“低促高抑”的浓度效应,与CK相比,T1对菘蓝生长具有一定的促进作用,而浸提液浓度超过菘蓝的耐受阈值时,会对其生长产生不利的影响。随着浸提液浓度的升高,可溶性糖和可溶性蛋白含量呈先上升后下降的趋势,而脯氨酸含量则持续增加。与CK相比,T1的MDA含量、细胞膜损伤率和H₂O含量差异不显著,而T3、T4则显著升高。茶树凋落叶浸提液对抗氧化酶活性及其基因表达有不同的影响,T1的过氧化物酶(POD)与抗坏血酸氧化物酶(APX)活性最高,T2的过氧化氢酶(CAT)活性最高,而T3、T4的4种抗氧化酶活性均显著低于CK。T1的Pod、Cat、Apx基因表达量最高,而T4则抑制了抗氧化酶基因的表达。此外,POD活性与其基因表达量呈显著正相关,而超氧化物歧化酶(SOD)、CAT和APX活性与其基因表达量的相关性不显著。不同浓度的茶树凋落叶浸提液对于菘蓝次生代谢物质积累的影响存在显著差异,低浓度茶树凋落叶浸提液对菘蓝生长、靛蓝和靛玉红含量积累有促进作用,而高浓度茶树凋落叶浸提液对总黄酮含量积累有一定的促进作用。本研究结果可为幼龄茶园中茶树-菘蓝复合种植提供理论参考。  相似文献   

Microbial remediation of a pentachloronitrobenzene-contaminated soil under Panax notoginseng: A field experiment     

Ning ZHANG  Dong GUO  Ye ZHU  Xiaomi WANG  Lingjia ZHU  Fang LIU  Ying TENG  Peter CHRISTIE  Zhengao LI  Yongming LUO 《土壤圈》2020,30(4):563-569

Pentachloronitrobenzene (PCNB) is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop seedlings. Microbial removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues. A short-term field experiment (1 year) was conducted to explore the potential role of a PCNB-degrading bacterial isolate, Cupriavidus sp. YNS-85, in the remediation of a PCNB-contaminated soil on which Panax notoginseng was grown. The following three treatments were used:i) control soil amended with wheat bran but without YNS-85, ii) soil with 0.15 kg m^-2 of solid bacterial inoculum (A), and iii) soil with 0.30 kg m^-2 of solid bacterial inoculum (B). The removal of soil PCNB during the microbial remediation was monitored using gas chromatography. Soil catalase and fluorescein diacetate (FDA) esterase activities were determined using spectrophotometry. In addition, cultivable bacteria, fungi, and actinomycetes were counted by plating serial dilutions, and the microbial biodiversity of the soil was analyzed using BIOLOG. After 1 year of in-situ remediation, the soil PCNB concentrations decreased significantly by 50.3% and 74.2% in treatments A and B, respectively, when compared with the uninoculated control. The soil catalase activity decreased in the presence of the bacterial isolate, the FDA esterase activity decreased in treatment A, but increased in treatment B. No significant changes in plant biomass, diversity of the soil microbial community, or physicochemical properties of the soil were observed between the control and inoculated groups (P<0.05). The results indicate that Cupriavidus sp. YNS-85 is a potential candidate for the remediation of PCNB-contaminated soils under P. notoginseng.  相似文献