Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat     

ZHANG En-guang  ZHANG Huai-qin  LIN Jie  HUANG Wei-jian  LI Shang-gong  XU Li-xin 《园艺学报》2003,19(5):653-656

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.  相似文献   

Oxidized low density lipoprotein increases the expression of matrix metalloproteinase-2 in vascular endothelial cells     

HAO Feng  YU Jin-de 《园艺学报》2003,19(6):733-736

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Role of vascular remolding induced by nitric oxide and matrix metalloproteinase in aneurysm     

LIAO Ming-fang  JING Zai-ping  ZHAO Jun 《园艺学报》2004,20(7):1326-1328

Nitric oxide (NO) and matrix metalloproteinases (MMPs) are important in vascular remolding, especially in abdominal aortic aneurysm. NO may be associated with aneurysms by modulating MMPs expression and activity.  相似文献   

Down-regulated TCF3 expression inhibits growth and migratory abilities of non-small cell lung cancer cells     

WANG Shi-meng 《园艺学报》2017,33(2):289-296

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自卸车举升机构矩阵变换算法统一格式的研究   总被引：1，自引：1，他引：1  

张毅  马力  王元良  满开美  李鹏飞 《拖拉机与农用运输车》2005,(1):21-23

针对自卸车各种举升机构提出了矩阵变换算法的统一格式,推导出统一格式下各举升机构的变换矩阵,并开发了通用设计系统。该系统可计算任一举升时刻举升机构各铰接点位置和油缸推力。  相似文献   

Demography,population dynamics and sustainability of the Patagonian sheep flocks     

Marcos Texeira  Jose M. Paruelo 《Agricultural Systems》2006

Sheep production is the main agricultural activity in Patagonia. Since the middle of the 20th century, sheep numbers have declined steadly. We used historical records of stock numbers in four ranches to analyze the importance of regional factors so as to explain the decline of the Patagonian sheep flocks. We found that the stocks of all the four ranches declined with a similar trend but fluctuated independently, thus reflecting a complex interaction between regional and local factors. Aboveground net primary production (ANPP) and vegetation physiognomy explained most of the differences in the flocks declining rates. We estimated demographic parameters for two ranches differing in their average annual growth rates. From these demographic parameters, we constructed deterministic and stochastic matrix models to establish the relative contribution of demographic processes to the observed decline. Matrix models projected a faster decline than that observed in the ranch used to calibrate the model. This suggests that the recorded demographic parameters could drive most stocks to extinction in less than 100 years. We concluded that the observed dynamics would be generated by demographic processes, but extinction is delayed or avoided by a continuous intake of animals. Ewe survival was the most important parameter in controlling the growth rate of the flocks. The environmental stochastic model showed that the growth of the stocks was highly sensitive to increases in the frequency of good years (those that produce a positive growth) and in the transition from normal years to bad years. All these evidences point out the existence of biological constraints to sheep production in Patagonia: ANPP and vegetation structure would control flock population dynamics throughout its effects on key demographic parameters, ewe survival and marking rate (a recruitment measure). Our model results suggest that the decline in sheep numbers, and hence the sustainability of the activity, is driven, to a large extent, by the demographic characteristics of the flocks.  相似文献   

Effects of pirenzepine on expression of MMP-2 and TIMP-2 in stroma of sclera in chick myopic eyes     

DAI Shu-zhen  ZHANG Li  WANG Li-ya  ZENG Jun-wen 《园艺学报》2012,28(8):1494-1499

AIM:To observe the effect of intravitreal injection of pirenzepine on form-deprivation myopia in chicks. METHODS:Forty-one day old chicks were randomly divided into 4 groups:normal control group, form deprivation group, vehicle control group and pirenzepine injection group. The right eyes of all chicks were used as experimental eyes. The deprived eyes in vehicle control group and pirenzepine injection group received daily intravitreal injection of vehicle control solution(0.01 mol/L PBS) and pirenzepine(1%in PBS), respectively. Optical examinations such as refraction, axial length and equatorial diameter were made at the end of the 5th day. The mRNA and protein levels of matrix metalloproteinase 2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2), and the activity of MMP-2 were detected by RT-PCR, Western blotting and zymography analysis,respectively. RESULTS:Refraction, axial length and equatorial diameter of the eyes in pirenzepine injection group were significantly lower than those in form deprivation group and vehicle control group(P<0.05), but those were higher(P<0.05) and the eyes were relatively myopic as compared with normal control group. The mRNA expression, protein le vels and activity of MMP-2 in pirenzepine group were significantly higher than those in normal control group(P<0.01), and were significantly lower than those in form deprivation group and vehicle control group(P<0.01, P<0.01). The mRNA and protein levels of TIMP-2 in pirenzepine group were significantly lower than those in normal control group(P<0.01), and was significantly higher than those in form deprivation group and vehicle control group(P<0.01, P<0.01). CONCLUSION:Intravitreal injection of pirenzepine may partly prevent form-deprivation myopia by modulating the expression of MMP-2 and TIMP-2 in the fibrous layer of sclera.  相似文献   

Factors Associated with Grassland Bird Species Richness: The Relative Roles of Grassland Area, Landscape Structure, and Prey     

Tammy L. Hamer  Curtis H. Flather  Barry R. Noon 《Landscape Ecology》2006,21(4):569-583

The factors responsible for widespread declines of grassland birds in the United States are not well understood. This study, conducted in the short-grass prairie of eastern Wyoming, was designed to investigate the relationship between variation in habitat amount, landscape heterogeneity, prey resources, and spatial variation in grassland bird species richness. We estimated bird richness over a 5-year period (1994–1998) from 29 Breeding Bird Survey locations. Estimated bird richness was modeled as a function of landscape structure surrounding survey routes using satellite-based imagery (1996) and grasshopper density and richness, a potentially important prey of grassland birds. Model specification progressed from simple to complex explanations for spatial variation in bird richness. An information-theoretic approach was used to rank and select candidate models. Our best model included measurements of habitat amount, habitat arrangement, landscape matrix, and prey diversity. Grassland bird richness was positively associated with grassland habitat; was negatively associated with habitat dispersion; positively associated with edge habitats; negatively associated with landscape matrix attributes that may restrict movement of grassland bird; and positively related to grasshopper richness. Collectively, 62% of the spatial variation in grassland bird richness was accounted for by the model (adj-R² = 0.514). These results suggest that the distribution of grassland bird species is influenced by a complex mixture of factors that include habitat area affects, landscape pattern and composition, and the availability of prey.  相似文献   

Effects of estrogen on expressions of MMP-2,MMP-9 and tissue inhibitors in prostate stromal cells     

XIAO Xiang-qian  FU Rong  ZHANG Li-guo  LIU Shu-ye  WU Quan  YAN Chun-he  SHI Jian-dang  ZHANG Ju 《园艺学报》2007,23(11):2248-2252

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Urban permeability for birds: An approach combining mobbing-call experiments and circuit theory     

《Urban Forestry & Urban Greening》2016

The urban matrix was recently shown to be a mosaic of heterogeneous dispersal habitats. We conducted a playback experiment of mobbing calls to examine the probabilities of forest birds to cross a distance of 50 m over urban matrix with different land-cover types in an urban area. We treated the reciprocal of the crossing probabilities as a movement resistance for forest birds. We drew resistance surfaces based on the land-cover maps of urban Sapporo. We applied a circuit theory to examine the relative role of a detour route consisting of a riparian corridor and urban matrix for dispersing forest bird individuals from continuous forest to an isolated green space in the midst of an urban area. Our results showed that wood cover had the highest crossing probability, while open land (grassland and pavement) had the lowest probabilities. Buildings and water surface displayed an intermediate probability. Resistance surfaces and flow maps at 25- and 50-m resolutions were very similar and suggested that dispersing individuals are likely to use the intervening building areas that dominate the urban matrix rather than detour through riparian corridors. Our results showed the useful combination of experimental approaches and circuit theory, and the importance of the spatial configuration of corridors, as well as the composition and management of dispersal habitats, to landscape connectivity.  相似文献