排序方式： 共有27条查询结果，搜索用时 15 毫秒
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[目的]为区分不同产地大黄药材的质量及鉴别提供依据。[方法]将供试材料青海玉树掌叶大黄（样品1）、青海海南唐古特大黄（样品2）与青海果洛唐古特大黄（样品3）分别粉碎成粉末,过100目筛,即可供X射线衍射试验用。设定管压30 kV,管流20 mA,2θ扫描范围3°～90°,扫描速度0.06°/s,每种样时间0.5 s,获得3种不同产区大黄的X-射线衍射图谱。试验数据以晶面间距d与特征峰相对强度L/Io表示,记为d/（I/Io）。将试验数据导入中药指纹图谱相似度计算软件,经选峰,设定匹配模板,将谱峰自动匹配,然后设定标准模板,进行谱峰差异性评价和整体相似性评价。[结果]样品1中所含的化学成分与样品2相同,但峰的强度（I/Io）不同,表明相同成分在两种样品中的含量不相等。同样把样品3的图谱与其他两个样品进行比较,也会得到相同的衍射峰值。表明不同产区的大黄中各化学成分的含量有差异,但其衍射图谱及衍射峰值具有一定的指纹特征。[结论]X-射线衍射法是鉴别不同产区的大黄及其他中药材的一种快捷有效的方法。 相似文献
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青海省黄南、果洛两州建立大黄人工种植基地,旨在研究和掌握野生大黄的生物学特性及生长发育规律,积极推广"人工育苗、自然生长"的生产模式及产业化运作模式,发挥产地中藏药资源优势,在逐步满足日益增长的市场需求,这将使以牧区扶贫和草原生态治理为目标的产业化项目实现预期的目的。 相似文献
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AIM:To approach the relationship between the expression of intercellular adhesion (ICAM-1 mRNA) and acute lung injury (ALI) as well as the mechanisms of rhubarb in the prevention and treatment of the lung injury. METHODS:ALI animal model was performed by Lipopolysaccharide (LPS). The rats were divided into 4 groups: LPS group, control group, rhubarb+LPS group and dexamethasone+LPS group. Histopathological examination and biological markers were measured for the lung specimens. Molecular hybridization method was used to determine the expression of ICAM-1 mRNA. RESULTS:The ICAM-1 mRNA expression in the lung tissues of LPS group significantly increased compared with control group (P＜0.01), rhubarb and dexamethasone had the action of decreasing the ICAM-1 mRNA expression (P＜0.05, P＜0.01); pathologic changes and the biological markers of ALI significantly decreased or ameliorated. CONCLUSION:The increase in the expression of ICAM-1 mRNA in the lung tissues of ALI is involved in the formation of ALI. Rhubarb and dexamethasone can ameliorate the lung damage, mechanism of which may be related to the inhibition of ICAM-1 mRNA expression. 相似文献
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[目的]建立HPLC法同时测定大黄中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量,并对不同产地的大黄样品进行测定。[方法]采用依利特ODS2 C18柱(200 mm×4.6 mm,5μm),流动相为无水甲醇-浓度0.1%磷酸(75∶25,V/V),流速为1 ml/min,检测波长为254 nm,柱温25℃。[结果]芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性回归方程分别为:Y=1.96X-0.25,相关系数R=0.999 94;Y=2.45 X+0.18,相关系数R=0.999 94;Y=1.91 X+0.05,相关系数R=0.999 90;Y=1.25 X-0.06,相关系数R=0.999 95;Y=0.96 X-0.20,相关系数R=0.999 98;平均回收率分别为99.39%、98.84%、98.94%、99.11%和98.37%。[结论]该方法简单、快速、重现性好、结果准确,可用于大黄药材品质评价与质量检测。 相似文献
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Variations in flower and seed and meiotic chromosome behaviors were investigated in micropropagated rhubarb (Rheum rhaponticum L.) ‘PC49’. The results showed that micropropagated plants had significantly higher variations in flower and seed morphologies and chromosome behaviors than plants derived from crown division. The results demonstrated that micropropagated plants grew 2–5 stamens, 8–13 stigmas, and 2–7 seed-wing, compared to 3 stamens, 9 stigmas and 3 (rarely 4) seed-wing in crown-derived plants. Additionally, alteration in chromosome behaviors (i.e. lagging and bridging) was observed only in micropropagated plants. All the results suggest that somaclonal variation may have been involved in micropropagated rhubarb ‘PC49’. 相似文献
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目的:研究中药大黄对蛋鸡产蛋性能及蛋品质的影响。方法:选择体重及生产性能相近的430日龄健康罗曼蛋鸡48只,随机分为对照组和试验组2组,每组设4个重复,每个重复6只鸡。其中A组为对照组,饲喂基础日粮,B组为试验组,在基础日粮中添加1%的中药大黄。预试期1周,正试期为6周。结果:试验结果表明2组间的产蛋率、蛋壳颜色、平均蛋重、蛋形指数、蛋黄指数、软破壳蛋率、料蛋比、采食量、哈夫单位差异不显著(P0.05)。与A组相比,B组蛋的比重显著增加(P0.05),蛋壳厚度虽有所增加,但差异不显著(P0.05)。蛋黄颜色改善极显著(P0.01),灰分含量极显著下降(P0.01),脂肪含量显著降低(P0.05),蛋白质含量升高,但差异不显著(P0.05)。结论:在饲料中添加1%的中药大黄对蛋鸡产蛋性能没有显著的影响,但能显著的改善鸡蛋的物理特性。 相似文献
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不同粒径大黄中有效成分溶出度对比 总被引：1，自引：0，他引：1
目的：测定不同粒径大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的溶出量。方法：用高效液相色谱法测定其溶出量。结果：随着粒径变细,其溶出量都在增加,但粉碎到300目以上时,其溶出量反而下降。结论：大黄超微粉的应用以200目效果最好。 相似文献
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AIM: To investigate the effect of compound rhubarb preparation (Kintop) in preventing obesity in rats and its probable mechanism involved. METHODS:Twenty-six newborn SD rats were randomly grouped as rhubarb preparation plus high-energy forage group(n=8), high-energy forage control group(n=8) and ordinary forage control group(n=10). The rats in rhubarb preparation plus high-energy forage group and high-energy forage control group were fed with high-energy forage and those in ordinary forage control group were fed with ordinary forage. The rats in rhubarb preparation plus high-energy forage group were administered by compound rhubarb preparation (40 mg·100 g-1 body weight·d-1) from 9th to 17th week. The dynamic changes in body weight, celiac fat weight and adipocytes size were measured. Immunohistochemical analysis of leptin in celiac adipocytes (ABC method) and measurement of serum leptin level (RID method) were performed. RESULTS:The body weight and the wet weights of celiac fat were lower, their adipocytes were smaller and immunohistochemical stainings of leptin were weaker in rhubarb preparation plus high-energy forage group than those in high-energy forage control group. There was an obvious positive correlation between the expression of leptin and celiac fat tissue weight(r=0.8663, P＜0.05). But the changes in serum leptin were not significant between groups. CONCLUSION:Compound rhubarb preparation (40 mg·100 g-1 body weight·d-1) can prevent the obesity in rats fed with high-energy forage. The mechanism might involve the decrease in adipocyte leptin expression. 相似文献