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京白梨等品种S 基因型鉴定及新基因S28 和S30 的核苷酸序列分析 总被引：8，自引：3，他引：8
根据梨S基因高度保守区C1和C3区, 设计1对引物P1和P2, 对梨品种的基因组DNA进行S基因特异扩增、克隆、测序, 并在GenBank中BLAST比较, 确定S 基因特异性片段, 对京白梨等6个供试自交不亲和品种的S基因型比对结果为: 白梨中的‘库尔勒香梨’为S21 S28 , ‘苹果梨’为S17S19 ; 砂梨中的‘台湾蜜梨’为S11 S22 ; 西洋梨中的‘葫芦梨’为Sa Sb; 秋子梨中的‘京白’为S16 S30 , ‘早梨18’为S4 S28。其中S28和S30为首次登录的新S 基因, 在GenBank的登录号分别为AY562394 (库尔勒香梨) 和AY876945 (京白) 。 相似文献
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K. Sharma A. M. Cachi P. Sedlák A. Skřivanová A. Wünsch 《The Journal of Horticultural Science and Biotechnology》2016,91(2):117-121
Sweet cherry (Prunus avium L.) is a self-incompatible species. Determination of the S-genotypes of cherry cultivars is crucial for breeding and to select appropriate cultivars for cross-fertilisation and fruit set. In this study, we characterised the S-genotypes of 25 sweet cherry cultivars, some of which had being bred at the Research and Breeding Institute of Pomology (RBIP), Holovousy, Czech Republic, and others were European cultivars in the RBIP collection. S-genotyping was carried out by polymerase chain reaction using consensus primers for the S-RNase and SFB genes, and capillary electrophoresis. Nine different known S-haplotypes (S1, S2, S3, S4, S5, S6, S9, S13, and S16) were identified and the cultivars were assigned to 12 incompatibility groups. One local cultivar, ‘Pta?ka z Plzně’, originated from a wild forest seedling and used as a pollinator, was assigned to Group 0 of universal donors. The pedigree of some cultivars was confirmed by their S-genotype. This study represents the ?rst comprehensive S-genotype screening of sweet cherry genetic resources in the Czech Republic and will be useful for the design of crossing programmes and orchard management of these sweet cherry cultivars. 相似文献
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Self-incompatibility of almond [Prunus dulcis (Mill.) D.A. Webb] is controlled by the S-locus with 30 described allelic variants. In this study, PCR amplification, cloning and DNA sequence analysis revealed a new S-RNase allele in a Hungarian cultivar, ‘Tétényi bőtermő’. This new allele was labelled as S31. Since S31 is characterized by almost identical intron sizes as S9, consensus PCR was not successful in discrimination of the alleles, even if fluorescently labelled fragments were sized on an automated sequencer. Therefore, an allele-specific forward primer (PdS31-F) was designed to anneal selectively within the second intron of the S31-RNase gene and used in combination with the EM-PC3consRD consensus primer. This allowed for the successful discrimination of S31 from S9. The PdS31-F primer and allele-specific PCR in general might be useful in the identification of different alleles with matching intron sizes that might occur during screening for S-alleles in a more diverse population, e.g. local cultivars from Central Europe to Asia. 相似文献
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