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苹果自花授粉的花粉管结构变化及S-RNase定位 总被引：4，自引：0，他引：4
苹果‘红星’品种授粉处理24 h后, 自花授粉比异花授粉的花粉管生长缓慢。授粉处理48 h后, 可观察到自花授粉花柱通道组织的花粉管内有二裂粒子增加、管壁破裂和管内物质溢出、外层管壁增厚等现象。苹果气球状花期未授粉的花柱及自花和异花授粉24 h后且无花粉管存在的花柱切片显示, S-RNase位于花柱通道组织的细胞内。自花和异花授粉48 h后有花粉管通过的花柱切片显示, S-RNase位于花柱通道组织的细胞间隙和花粉管内。 相似文献
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Due to the several advantages expected from growing self-compatible almond cultivars, breeding programmes have recently introduced self-compatibility as an important trait. However, doubts have arisen regarding the capability of the newly obtained self-compatible almonds to produce good yields in monovarietal plantations, and the commercial quality of the fruits from self-pollination. For this reason, in the last years several studies have been conducted to evaluate fruit set and fruit traits following self- and cross-pollination of self-compatible almonds. Their results showed inconsistencies for fruit set and for some of the fruit traits studied. Thus, while some studies showed no differences between both pollination types for any of the fruit traits, others clearly showed important differences with the detriment of self-pollination for commercially important fruit traits. The fact that some of these studies evaluated a low number of individuals and/or fruit traits, implies the need of a more complete study. In this work, 10 fruit traits were studied in 26 self-compatible almonds following self-pollination by bagging and open pollination treatments. In general, and despite a few individuals showed differences between both pollination types for two of the traits, the results showed no influence of self-pollination in fruit quality. 相似文献
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新型甘蓝型油菜核不育材料绵7AB-4-2农艺性状鉴定及分子检测 总被引：3，自引：1，他引：2
四川省绵阳市农科所1991年用甘蓝型油菜双高隐性核不育系绵1AB-1作母本,品872—161—134作父本进行转育,F2代出现不育株进行成对兄妹交,经多代选择,培育出隐性核不育系绵7AB-4（双低）。以其不育系的不育性状为选择目标,从中分离纯化出2个农艺性状无明显差异、育性不同的株系,分别定为绵7AB-4-1和绵7AB-4-2,前者兄妹交和可育株自交的不育率分别为50％和25％,后者分别达到80％和60％。笔者对新材料绵7AB-4-2与其姊妹系绵7AB-4—1的农艺性状和SSR引物多态性进行研究,为其进行分子标记、克隆及进一步利用奠定基础。 相似文献
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为探究外源茉莉酸甲酯(methyl jasmonate, MeJA)对小麦温敏雄性不育系BNS366雄性育性的影响,以BNS366及其近等基因系郑麦366为试验材料,在正常秋季播种(2020年10月12日)和晚播(2020年12月2日)条件下,分别于2021年3月22日和3月31日开始至开花期前,设置0(清水,对照)、50、100、200、300、400和500μmol·L-1 MeJA(水溶液,喷施)7个处理,比较分析了不同处理间花粉可育率和自交结实率的差异。结果表明：在正常秋季播种条件下,郑麦366花粉可育率、国内法自交结实率和国际法自交结实率分别为86.31%、70.36%和112.22%,在晚播条件下分别为82.53%、92.53%和166.18%,均正常可育;在正常秋季播种条件下,200μmol·L-1 MeJA处理的国际法自交结实率为70.15%,比对照显著降低了42.07个百分点;在晚播条件下,200、300和500μmol·L-1 MeJA处理的国内法自交结实率分别为74.71%、74.01%和73.45... 相似文献
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[Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4-2 in Brassica napus. [Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB-4-1 from test cross and back cross were also studied. [Result] Mian 7AB-4-2 was not significantly different in agronomic traits from its sisterly line Mian 7AB-4-1 at 0.05 probability level. The percentages of sterile individuals in the pollinated fertile Mian 7AB-4-2 plants were over 60%, and that in its sisterly line Mian 7AB-4-1 was about 25%. In test crosses with other nine sterile lines, Mian 7AB-4-1 kept the percentage of sterile individuals of sterile lines over 90%, and the percentage of sterile individuals from back cross over 80%. With regard to molecular markers, Mian 7AB-4-2 and its sisterly line Mian 7AB-4-1 were different in the band number from SSR primers a2 and E10. [Conclusion] The results indicate that Mian 7AB-4-2 is helpful for rapeseed breeding, quickening the application of new materials in field breeding. 相似文献
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四倍体西瓜低稔性胚胎发育研究 总被引：1，自引：1，他引：0
试验以二倍体自交为主要对照，四倍体与二倍体正反交为辅助对照，研究了四倍体西瓜自交低稔性的胚胎发育。通过镜检观察表明：1．四倍体西瓜自交花粉萌发率低，花粉管在柱头与花柱中生长出现异常现象，在花柱中生长缓慢，授粉后12h到达花柱基部。2．四倍体西瓜有少数结构不正常胚囊，但所占比率低于10％，不是稔性低的主要原因。3．四倍体自交双受精过程正常，但花粉管进入的胚囊数少，受精率仅35．8％。4．四倍体自交只有少数胚能正常发育，形成有活力种胚；大部分胚由于游离核或细胞化胚乳的异常解体，在原胚或球形胚阶段败育；少数胚分化到心形胚、鱼雷胚后，由于细胞化胚乳过早解体，胚停止分化而败育。 相似文献
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