Transcriptome Analysis of Myzus persicae to UV-B Stress     

Chang-Li Yang  Jian-Yu Meng  Meng-Shuang Yao  Chang-Yu Zhang 《Journal of insect science (Online)》2021,21(3)

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Identification,characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)     

Wen-wen LIU  Min XIN  Meng-ji CAO  Meng QIN  Hui LIU  Shou-qi ZHAO  Xi-feng WANG 《农业科学学报》2018,17(10):2281-2291

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA(dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus(HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region(UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame(ORF) that encodes a deduced 4 867 amino acids(aa) polyprotein with three domains: RdRP, Hel and UGT(UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization(FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.  相似文献   

水稻条纹病毒在玉米和水稻上流行差异分析     

程兆榜  陈思宏  朱荣鹏  刘海建  魏邦庆  熊如意  周彤  季英华  周益军  范永坚 《勤云标准版测试》2006,(12)

为了明确水稻条纹病毒(RSV)在水稻和玉米上发生程度差异明显的原因,从一个侧面了解RSV流行本质,2004年在洪泽进行田间试验。采用黄盘诱集、盘扑、盘刮、肉眼计数等方法比较武育粳3号和掖单13上灰飞虱侵入和消长动态,结果表明两者均只有一个成虫侵入高峰,单位面积迁入虫量前者是后者的3.6倍,单株平均虫量相近。Dot-ELISA法测定灰飞虱带毒率为40%,成虫迁移扩散高峰期22d内两者接毒量约为每天百株137头带毒虫。武育粳3号有二代若虫发生,掖单13则无。逐日调查发病进程,结果显示水稻发病率为60%,玉米为0。室内抗性鉴定,掖单13对RSV的抗性比武育粳3号高4个级别。综合以上结果,寄主品种抗性决定了RSV在水稻和玉米上流行状况,二代若虫重复侵染是RSV在水稻上重发的另一主要原因。  相似文献   

论电路中的干扰形成及干扰抑制技术     

王万刚 《农业网络信息》2006,(5):126-128

本文对电路中的噪声的形成进行分析,并对噪声干扰的抑制技术进行探讨。  相似文献   

T7 RNA多聚酶基因的克隆、序列测定及其在E.coli中的表达     

余兴龙  涂长春  徐兴然  张茂林  张青婵  李作生 《中国兽医学报》2003,23(5):452-454

从BL21(DE3)E．coli菌株中以PCR的方法扩增得到了与T7RNA多聚酶(T7RNApolymerase，T7pol)基因大小一致的DNA片断。将PCR产物纯化后直接克隆到pGEM—T载体中，经酶切鉴定和DNA序列分析表明克隆得到了正确的T7pol基因。将T7pol基因亚克隆入pET-28b( )中，构建得到原核表达质粒pET28T7。该质粒的BL21(DE3)pLysS转化菌在IPTG的诱导下可表达约98800的蛋白，这与T7pol的相对分子质量一致。将该质粒转化DH5α、JMl09、HBl01、BL21(DE3)和BL21(DE3)pLysS等5种不同的宿主菌，仅有转化T7pol酶活性受到抑制的宿主菌BL21(DE3)pLysS才能得到转化子，而其余4种T7T7pol酶活性不受抑制的E．coli宿主菌不能得到转化子。pET28T7原核表达质粒这种仅能在T7pol酶活性受到抑制的宿主菌中才能存活的现象说明本试验所克隆的T7pol基因能正确表达出具有RNA转录酶活性的蛋白。  相似文献   

加工现场微机控制系统抗干扰的研究     

刘少凤  陈友明 《湖南农业大学学报(自然科学版)》1996,23(6)

针对制造业加工现场的环境特点，分析了微机控制系统的干扰来源和途径，介绍了实用的抗干扰措施。实践证明：这些软硬件抗干扰措施的综合运用，使自动化加工中微机控制系统的稳定性得到了较大的提高。  相似文献   

The RNA interference     

KONG Xiang-ping  Lixing W. Reneker 《园艺学报》2004,20(5):900-903

The phenomenon of RNA interference (RNAi) is highly conserved mechanism in the organism evolution. As a immune system, RNAi is a ubiquitous mechanism against invading microorganism in plant and animal cells. Recently, it has been found that RNAi is the process by which double-strand RNA(dsRNA) directs sequence-specific degradation of messenger RNA and the mediations of sequence specific messenger RNA degradation are 21-and 23-nucleotide small interfering RNAs that generate by ribonuclease from endogenous longer dsRNA or by transfectious technics from heterologous dsRNA. Over the past few years, the way in which cells respond to dsRNA by silencing homologous genes has revealed a new regulating paradigm in biology.  相似文献   

近冰点温度贮藏草莓的研究     

郝利平  郝林 《果树学报》1993,(1)

草莓呼吸旺盛,果皮薄易受伤,难以贮藏。利用近冰点温度贮藏可以明显地抑制草莓的呼吸作用,结合小袋包装的气密性,改善了包装内部的气体组成,提高草莓果实周围的相对湿度并减少空气对流,从而抑制了草莓在贮藏过程中糖分、水分的损耗,减少了糖酸比值的下降。保持了一定的感观指标,可使草莓的贮藏时间延长。  相似文献   

Study on ambient noise in sawmill’s workshop     

Liu Dali 《林业研究》1996,7(3):73-76

Ambient noise in 13 sawmill’s workshops was tested and analyzed to reveal its character and regularity. The situation of the ambient noise in sawmill’s workshops was evaluated  相似文献   

人工培育牛黄对小白鼠离体小肠运动的解痉作用     

贺春阳  关红 《内蒙古农业大学学报(自然科学版)》1989,10(1):31-36

本文证明培育牛黄对乙酰胆硷与氯化钡所引起的小白鼠离体小肠痉挛有解痉作用。培育牛黄的成份中去氧胆酸具有较强的抗乙酰胆硷与氯化钡作用,其浓度与效应呈直线相关。其成分中的胆酸仅有较弱的抗乙酰胆硷作用,且浓度与效应不呈直线相关,也无抗氯化钡作用。  相似文献