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为明确北京地区南瓜病毒病种类及其主要侵染病原,2016~2017年在北京周边采集疑似感染病毒的南瓜病样84份,并根据南瓜上的6种病毒特异性引物对其进行反转录PCR(RT-PCR)检测。结果表明:共有79份南瓜病样检测显示阳性,其中黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为52.38%;其次是小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)和西瓜花叶病毒(Watermelon mosaic virus,WMV),检出率分别为44.01%、14.29%,其他病毒暂未检出。此外,16.67%的样品受2种病毒复合侵染,CMV和ZYMV复合侵染占7.14%,ZYMV和WMV复合侵染占9.52%。北京地区南瓜上优势病毒种类为CMV,且存在病毒复合侵染现象。 相似文献
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南瓜种质资源亲缘关系的RAPD分析 总被引：6，自引：0，他引：6
南瓜是国内外广泛栽培的重要蔬菜作物之一,其种质资源十分丰富。开展南瓜的遗传多样性和亲缘关系的研究,有利于了解南瓜遗传多样性的分布和种质间的遗传关系,促进南瓜种质的收集和有效利用。以不同来源地、不同生态型的南瓜种质资源为试材,用CTAB方法从南瓜幼嫩叶片提取基因组DNA,用长度为10个碱基的寡核苷酸作RAPD引物进行PCR扩增反应。从260个随机引物筛选出26个多态性引物,对76份南瓜种质资源进行扩增,共扩增出255条带,其中多态性带为207条,多态性百分率为81.18%。表明南瓜种质资源遗传多样性丰富。聚类分析将供试材料分为3大类:中国南瓜、美洲南瓜和印度南瓜,种间差异明显。 相似文献
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[目的]研究土壤调理剂对大棚西葫芦生长发育及产量、品质的影响。[方法]设3个处理,分别为0(CK)、15(A1)2、5 g/株(A2),在生长期间调查植株的座果数、株高、叶绿素含量等生物学性状,始收时统计始收期、始花期,分析测定各处理西葫芦果实的总糖、还原糖、可溶性固形物、Vc含量,比较处理对西葫芦果实品质的影响,调查化瓜率、病果率、白粉病发病率、病毒病发病率、死株率等。[结果]结果表明,增施土壤调理剂使植株抗逆性增强、产量和品质显著提高。与对照相比,大棚内增施土壤调理剂使西葫芦株高增加24.4%,根系活力增加36.3%,叶面积增加25.8%,产量增加35.5%,Vc增加20.1%,差异均达到显著水平。[结论]综合比较不同处理对西葫芦生长发育的影响可知:土壤调理剂用量在25 g/株表现最佳。 相似文献
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Seed samples of 174 accessions of pumpkins, squash, and gourds of Cucurbita pepo were measured for length, width, and thickness. Mean seed length ranged from 8.8 to 23.3 mm, mean seed width from 5.0 to 12.5 mm and mean seed thickness from 1.2 to 3.8 mm. The seeds of the edible-fruited cultivar-groups of C. pepo subsp. pepo (Pumpkin, Vegetable Marrow, Cocozelle, Zucchini) were longer, wider, and thicker than those of their counterparts of C. pepo subsp. texana (Acorn, Crookneck, Scallop, Straightneck). The small-fruited, inedible gourds had the smallest seeds. Of the edible-fruited C. pepo subsp. pepo, the Pumpkin Group (round fruits) possessed the longest and flattest seeds whilst the Zucchini Group (long, uniformly cylindrical fruits) possessed the shortest and thickest seeds. The samples tested also differed greatly in mean values for relative dimensions. Length-to-width ratio ranged from 1.5 to 2.5, length-to-thickness ratio from 3.7 to 8.2, and width-to-thickness ratio from 2.0 to 4.9. The Pumpkin Group, followed by the Cocozelle Group (long, bulbous cylindrical fruits) and the Vegetable Marrow Group (short, tapered cylindrical fruits), had seeds of the highest length-to-width ratio. The seeds of the four edible-fruited groups of C. pepo subsp. pepo differed significantly from one another in their length-to-thickness and width-to-thickness ratios. The seeds of the Pumpkin Group and the wild Mexican gourd (C. pepo subsp. fraterna) differed greatly in their dimensions but were most similar to one another in their relative dimensions. In C. pepo, seed size is positively associated with fruit size and seed shape is inversely associated with fruit shape. 相似文献
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[目的]为西葫芦的高产栽培提供理论依据。[方法]选用已经保存4年的西葫芦品种西葫芦1号种子为供试材料,用不同浓度的青霉素、多效唑进行浸种处理,并采用青霉素200 mg/L分别和不同浓度多效唑混配处理,以清水为对照,进行室内发芽和田间种植试验,研究青霉素和多效唑对西葫芦陈种子发芽及田间成苗的影响。[结果]用青霉素200 mg/L、多效唑500 mg/L处理对西葫芦种子发芽率、幼苗根系活力、叶片叶绿素含量和田间成苗效果较好,二者复配青霉素200 mg/L＋多效唑500 mg/L处理效果最好,与对照差异达到显著水平。[结论]选择适宜浓度的青霉素、多效唑以及二者复配,可促进西葫芦种子新陈代谢系统的修复和恢复,使幼苗达到正常常规种子生长的指标,建议在农业生产实践中应用。 相似文献
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