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1.
T型雄性不育小麦种子蛋白的电泳分析 总被引：7，自引：0，他引：7
以梯度SDS－PAGE方法分析了T型小麦雄性不育系及其保持系种子醇溶蛋白和谷蛋白组分，发现两系间有明显差异，而且四个不同品种小麦不育系均有各自与保持系区别的谱带出现，表明T型雄性不育小麦醇溶蛋白基因的表达有品种特异性。用单、双向电泳结合银染技术进一步分析了胚乳醇溶蛋白，发现不育系11A和14A，分别有24kD和36kD的特异蛋白。 相似文献
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FU Run-xi YANG Ruo-song HUANG Xue-qin HE Wen-ming YU You-yan WEI Deng-ming 《园艺学报》2018,34(6):996-1001
AIM: To observe the effect of thyroxine on the expression of T-type calcium channels Cav3.1, Cav3.2 and Cav3.3 in rat myocardium, and to explore the possible biological mechanism between the changes of the expression of T-type calcium channels and the arrhythmia in hyperthyroid heart disease. METHODS: Healthy SD rats (n=20) were randomly divided into normal control group (n=10) and hyperthyroid heart disease group (n=10). The animal model was established by intraperitoneal injection of levothyroxine for 35 d. The contents of T3 and T4 in serum, the heart-to-body weight ratio, the diameter of cardiac myocytes and electrocardiograph were measured to evaluate hyperthyroid heart disease. Moreover, the mRNA and protein expression levels of T-type calcium channels in the myocardium were measured by RT-PCR, immunohistochemistry and Western blot. RESULTS: After intraperitoneal injection of levothyroxine for 35 d, compared with the normal control group, the serum contents of T3 and T4, the heart-to-body weight ratio and the diameter of cardiac myocytes were significantly increased in hyperthyroid heart disease group (P<0.05), and arrhythmia occurred in hyperthyroid heart disease group. By immunohistochemistry and Western blot, the protein expression of Cav3.1 increased significantly (P<0.05), while the protein expression of Cav3.2 decreased significantly (P<0.01). However, no change of the Cav3.3 protein was observed. The results of RT-PCR were the same as immunohistochemistry and Western blot. CONCLUSION: Thyroxine promotes the expression of Cav3.1 in the myocardium but inhibits the expression of Cav3.2 at mRNA and protein levels, which might be involved in arrhythmia in hyperthyroid heart disease. 相似文献
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徐文其 《中国渔业质量与标准》2021,(2):38-43
为获取鲜活大菱鲆(Scophthalmus maximus)溯源标识固定工艺,分别采用细耳标扣、T型扣与套环扣进行鱼体固定试验,对比各组的钉扣固定速率与钉扣有效固定率,评价不同钉扣固定后鱼体状态指标,选出最合适的钉扣.在此基础上,对比鱼体不同部位鳍体的固定效果,并对钉扣固定位置范围参数进行优化试验.结果表明,采用T型扣... 相似文献
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以洋葱T型细胞质雄性不育系8-43A、保持系8-43B和恢复系8-43-57C为试材,采用萤火虫荧光素酶检测技术,分析花蕾不同发育时期三磷酸腺苷(ATP)的含量变化,研究洋葱T型细胞质雄性不育与花蕾ATP含量的关系。结果表明:在整个花药发育过程中的各个阶段,保持系和恢复系的花蕾之间ATP含量均无显著差异,不育系花蕾ATP含量均极显著低于保持系和恢复系,尤其在花粉粒成熟早期,仅为保持系和恢复系的1%;花粉粒成熟早期,不育系花蕾ATP含量下降到不足四分体时期的1/10,说明花药发育通过单核期后耗费了组织内大量的ATP,更重要的是ATP的产生可能出现了障碍;洋葱T型细胞质雄性不育系花蕾ATP含量极显著低于各时期的可育株,可能是导致其雄性不育的原因。 相似文献
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为研究T形板肋对预制带肋底板混凝土叠合板弯曲疲劳性能的影响,对3块T形肋底板叠合板和1块整浇板进行弯曲疲劳性能对比试验,主要考察T形板肋与疲劳荷载幅值对试件疲劳破坏形态及疲劳损伤程度的影响,得到了在不同疲劳循环加载次数下的跨中动位移、混凝土应变、预应力筋应变、残余变形等,分析了在不同疲劳循环加载次数下的刚度退化情况、荷载-应变分布规律、裂缝分布规律及剩余承载力等。研究结果表明,经历200万次疲劳循环加载后,T形肋底板叠合板无明显的刚度和强度退化,增设T形板肋的叠合板能达到与整浇板相同的弯曲疲劳性能;T形肋底板叠合板正截面弯曲疲劳强度计算可采用普通预应力混凝土受弯构件正截面疲劳应力验算的4个假定,最终以此建立了其正截面弯曲疲劳强度验算方法。 相似文献
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不同细胞质小麦雄性不育系及杂种F1叶绿体的观察与比较 总被引：1，自引：0，他引：1
【目的】研究CMS小麦叶绿体显微结构的特性,探讨CMS与叶绿体结构的关系,揭示CMS机理。【方法】以K、V、T型同核异质不育系（A）、保持系（B）冀5418、育性恢复的F1和各自的质供体粘果山羊草（Aegilops kotschyi）、偏凸山羊草（Aegilops ventricosa）、提莫菲维小麦（Triticum timopheevii）为试验材料,运用透射电镜技术分析研究旗叶叶绿体的特性及差异。【结果】（1）K、V、T型不育系旗叶叶绿体结构异常,表现为基粒片层之间界限模糊、消失,发育滞后,与基粒连接的类囊体不发达,甚至断裂,整个片层排列紊乱。保持系冀5418叶绿体紧贴细胞内膜,基粒片层清晰,排列整齐,基粒之间的类囊体清晰可见,发育完好。（2）保持系每个细胞的叶绿体数量为24.1个,形状为长椭圆形。K型不育系为19.8个,T型不育系为18.4个,与保持系差异显著（F=40.47,Pr＜0.0001）,叶绿体形状都呈圆形。V型不育系叶绿体数量为24.8个,显著高于其它两系（F=40.47,Pr＜0.0001）,与保持系差异不显著（F=40.47,Pr＜0.0001）,形状呈长椭圆形。（3）Aegilops kotschyi、Triticum timopheevii型质供体的叶绿体形状为圆形,Aegilops ventricos型质供体为椭圆形,叶绿体片层模糊,类囊体不发达,K、V、T型不育系叶绿体显微结构与其相似,来源一致。（4）K、T、V型不育系杂种叶绿体形状都呈椭圆形,紧贴细胞内膜,双层外膜清晰,基质浓厚,基粒片层清晰,且充满整个基质,基粒之间的类囊体清晰可见。【结论】K、T型雄性不育系叶绿体数目少于保持系,而V型叶绿体数目与保持系相当。三种不育系叶绿体结构、形状表现异常,具有明显的胞质效应;恢复基因不仅能恢复K、V、T型不育系的育性,且能使其叶绿体结构、形状恢复正常,具有生产应用价值。 相似文献
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5种鱼类标志对草鱼临界游泳速度的影响 总被引：1，自引：0，他引：1
采用被动整合雷达标志法(PIT)、切鳍标记、荧光标记、超声波标志和T型标志这5种标志方法对草鱼(Ctenopharyngodon idellus)进行标记后,测定其临界游泳速度,研究5种不同标记对草鱼游泳能力的影响。将试验草鱼按不同体长分为3组:15~18 cm,18~21 cm,21~25 cm,每个体长组均设置对照组,并分别进行以上5种鱼类标志,测量标志后草鱼的绝对临界游泳速度和相对临界游泳速度。采用SPSS17.0统计软件进行数据的分析比较。研究结果表明,随着体长增加,草鱼的绝对临界游泳速度增大,相对临界游泳速度减小;超声波标志对3个体长组草鱼的临界游泳速度均有极显著影响(P0.01),标志后草鱼绝对临界游泳速度分别下降18.72%、16.40%、23.15%,相对临界游泳速度分别下降18.95%、17.78%、21.86%;T型标志对15~18 cm体长组草鱼的临界游泳速度有极显著性影响(P0.01),标志后草鱼绝对临界游泳速度下降8.35%,相对临界游泳速度下降9.30%,对体长大于18 cm的草鱼无显著性影响;PIT标志、切鳍标记和荧光标记对这3个体长组草鱼的临界游泳速度均无显著性影响(P0.05)。 相似文献
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AIM: To investigate the effect of quercetin on endothelin-1-induced T-type calcium channel(TCC) expression in primary cultured human umbilical arterial smooth muscle cells for exploring the protective role of quercetin in cardiovascular system. METHODS: Human umbilical arterial smooth muscle cells were verified by immunocytochemistry. The cells in 2-3 passages were used and randomly divided into control group, quercetin alone group, model group and experimental group. The cells in control group were cultured without any drugs for 24 h. The cells in quercetin alone group were cultured with 80 μmol/L quercetin for 24 h. The cells in model group were cultured with ET-1 at the concentration of 100 nmol/L for 24 h. The cells in experimental groups were pretreated with quercetin for 1 h, then coincubated with 100 nmol/L ET-1 for 24 h. The concentrations of quercetin used in this study were 20, 40and 80 μmol/L, respectively. The expression of α1G, a TCC major subunit, was assayed at mRNA and protein levels by RT-PCR and Western blotting, respectively. The TCC currents(IcaT) were detected by the technique of whole-cell patch-clamp. RESULTS: Compared with control and experimental group, ICaT density (P<0.01) and the expression of α1G at mRNA (P<0.05) and protein (P<0.01) levels in model group were significantly increased. No significant difference in the results of quercetin alone group and control group was observed. CONCLUSION: The protective roles of quercetin in cardiovascular functions are related to the depressive effects of quercetin on ET-1-induced increase in both ICaT density and the expression of α1G at mRNA and protein levels in cultured human vascular smooth muscle cells. 相似文献
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Jumpei YASUDA Muneyoshi OKADA Hideyuki YAMAWAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1289-1291
Endostatin, a fragment of collagen XVIII, is known as an endogenous angiogenesis
inhibitor, and its serum concentration increases in various cardiovascular diseases.
T-type Ca2+ channel, low voltage-activated Ca2+ channel, is not
expressed in adult ventricular myocytes. Re-expression of T-type Ca2+ channels
in cardiac myocytes is thought to be involved in the development of cardiac hypertrophy.
We examined the effects of endostatin on T-type Ca2+ channel current by
whole-cell patch clamp technique in freshly isolated adult guinea pig ventricular
myocytes, which exceptionally express T-type Ca2+ channels. Although endostatin
300 ng/ml had no effect on L-type Ca2+
current, it significantly inhibited T-type Ca2+ current. These data indicate
that endostatin can be an endogenous inhibitor of T-type Ca2+ channels in the
cardiac myocytes. 相似文献