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M. Maślanka B. Prokopiuk 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2019,69(5):398-404
Here organogenesis of tarda tulip (Tulipa tarda Stapf.) from callus explants is presented. The callus tissue was cultivated on MS media containing 3% or 6% sucrose and either no addition of BAP (6-benzyl-aminopurine) or supplemented with 0.5 μM BAP. The cultures were maintained under a 16?h photoperiod under white, red or blue fluorescent light, at 20?±?2°C for 12 weeks. This study aimed to determine the most suitable light conditions and medium composition for seed-derived callus explants in order to obtain an efficient formation of adventitious bulbs. There were no differences between the spectra of light in differentiating adventitious bulbs. Explants cultured in darkness (control), on 0.5?µM BAP and 3% sucrose, formed the highest number of adventitious bulbs. The efficiency of adventitious organogenesis amounted to 36.6 bulbs per 1?g of callus tissue. The fresh weight of biomass, cultured in these conditions, increased within 12 weeks from 1 to 6.99?g. Supplementation with BAP of the medium containing 3% sucrose promoted the formation of bulbs, but in the case of the medium with 6% sucrose, BAP had an adverse influence under every type of light. The obtained results provide a useful protocol for the micropropagation of T. tarda, which can be used commercially for rapid and cost-effective production of the tulip. 相似文献
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该文简要介绍了郁金香花期调控的主要关键技术,总结近些年有关温度、光照和激素等调控郁金香花期的研究,阐明了郁金香花期调控技术研究的概况,并结合自身的研究实践,对郁金香花期调控研究存在的问题、未来的研究方向及其应用前景进行了展望。 相似文献
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郁金香引种栽培研究 总被引:2,自引:0,他引:2
从荷兰引进4个郁金香品种,调查并比较了各品种的植物学特性、物候期、生长发育规律和种球繁殖特性。结果表明:品种LargeCopper的种球最矮,茎上柔毛最长;品种Toronto的种球膜质鳞片为桔红色,无光泽,叶片上有红褐色条纹,种球最高、最重,内外轮花瓣长最长,花径、花高、雌蕊高、花药长和花丝长均最大,但内、外轮花瓣最窄;品种Pink Impression植株最高,叶片长而宽。供试4个品种郁金香均在4月上旬萌芽,1周后展叶,4月末现蕾,5月上旬开花。花期一般为10 d,品种Toronto的花期最短,仅为1周;品种Apricot Beauty花期最长,可达2周。4个品种在现蕾前后到盛花期生长迅速。品种Apricot Beauty的种球繁殖系数最高,达4.91。品种Pink Impression的一级子球产量较高,为31.1%。 相似文献
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[目的]探寻能满足郁金香生长发育需求的畜禽废弃物配施配方,应用于生产[方法]以泥炭、珍珠岩、鸡粪、猪粪、复合肥等基质为试材,研究不同畜禽废弃物配施的基质对盆栽郁金香生长发育的影响。[结果]以泥炭、珍珠岩、鸡粪、复合肥4种基质按6.5:2.4:1.0:0.1的比例和泥炭、珍珠岩、猪粪、复合肥4种基质按6.5:2.4:1.0:0.1的比例混配,有利于盆栽郁金香的生长发育[结论]选出了较适宜郁金香生长的基质配方:泥炭、珍珠岩、鸡粪、复合肥4种基质按6.0:2.4:1.0:0.1比例配制. 相似文献
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Jules Beekwilder Adèle van Houwelingen José van Beckhoven Arjen Speksnijder 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(4):477-485
For detection of the plant pathogenic Tulip virus X (TuVX), a panel of six recombinant antibodies was identified. To this end, a repertoire of variable domains from heavy-chain
immunoglobulins (VHH) was cloned from an alpaca, which had been immunized with TuVX. Binding domains were selected by phage
display and panning on immobilized TuVX particles. Recombinant VHH antibodies were tested for sensitivity in a sandwich ELISA,
and were demonstrated to be readily able to distinguish TuVX-infected tulip leaf material from uninfected leafs. No cross-reactivity
of the VHH antibodies to related flexiviridae was observed. Recombinant VHHs maintained their reactivity upon storage at −20°C for over a year. The effect of incubation
at higher temperatures for prolonged time was studied. Two out of three VHH proteins retained activity after several weeks
of storage at 37°C. 相似文献
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简述郁金香引种品种及其形态特征、生活习性,通过对当地气候、土壤条件的分析,探索在高寒地区的种植技术和管理方法,为今后格尔木市郁金香种球的繁育和栽培提供科学依据。 相似文献