Effect of cytokinin content of the regeneration media on in vitro rooting ability of adventitious apple shoots     

K. Magyar-Tábori  J. DobránszkiI. Hudák 《Scientia Horticulturae》2011

Rooting ability of ‘Royal Gala’ shoots regenerated on media with different cytokinin content (thidiazurone, benzyladenine, benzyladenine-riboside and meta-topolin-riboside) was observed directly after regeneration and compared after subculture on hormone-free medium (A), or on medium with decreased cytokinin content (B), or elevated gibberellic acid (GA₃) content (C) for a week, or after a subculture on proliferation media (1.0 mg l⁻¹ benzyladenine-riboside) for four weeks (treatment D). Rooting and acclimatization of regenerants after directly regeneration was not successful. Subculture of shoots on hormone-free medium did not improve the rooting ability of shoots, while their subculture on B and C media resulted in up to 36% rooting rate depending on regeneration media from which the shoots originated. The best rooting rate (up to 76%) was achieved in shoots regenerated on medium with benzyladenine-riboside after treatment D similarly to rooting ability of micropropagated shoots (80%). The rooting capacity of shoots depended on both the cytokinin content of the regeneration media and different subculture media used. All rooted shoots survived after acclimatization.  相似文献