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为了探讨草莓中单脱氢抗坏血酸还原酶（Monodehydroascorbate reductase,MDHAR）和谷胱甘肽还原酶（Glutathione reductase,GR）基因的功能,采用同源克隆的方法从‘丰香’草莓中克隆得到cDNA全长序列,并采用实时定量PCR方法对其在不同组织和不同发育阶段果实中的表达模式进行分析。结果表明：草莓MDHAR基因cDNA（FaMDHAR,GenBank登录号：KP025946）全长1 305 bp,编码434个氨基酸,分子量约为47 kD,含有保守的FAD结合结构域,定位于细胞质中。GR基因cDNA（FaGR,GenBank登录号：JQ339738）开放阅读框全长1 491 bp,编码496个氨基酸,分子量为53 kD,定位于细胞质中。FaMDHAR在各组织中均有表达,在成熟果实中表达量最高,叶和花次之,根中最低;在果实发育过程中FaMDHAR在绿果期有相对较高的表达,随后急剧增加,到白熟期最高,之后下降并维持在相对稳定水平。FaGR在叶和花中表达较高,在成熟果实中较低;在果实发育过程中,表达量从小绿期呈现增加趋势,至转红果期达最高,随后逐渐下降,在成熟果实中较低。果实发育过程中酶活性变化呈现出与各自基因表达量相似的变化规律。经过4 ℃低温处理24 h后的草莓叶片中,FaMDHAR相对表达量较对照显著增加,而FaGR无显著变化。草莓中FaMDHAR和FaGR表达存在时空差异,并对低温逆境响应存在差异。 相似文献
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To elucidate the physiological mechanism of heat stress mitigated by exogenous hydrogen peroxide (H2O2) further, seedlings of Cucumis sativus cv Lvfeng no. 6 were subjected to two temperatures (42/38 and 25/18 °C) after pretreatment with 1.5 mM H2O2. We investigated whether exogenous H2O2 could protect chloroplast ultrastructure under heat stress (42/38 °C) and whether the protective effect was associated with the regulation of antioxidant enzymes. The heat condition disintegrated the membranes of 71.4% chloroplasts in the leaf cells and resulted in the elevated levels of malondialdehyde (MDA) and endogenous H2O2. When H2O2 pretreatment was combined with the heat stress, the abnormal chloroplasts occurred at a rate of 33.3%, and the contents of MDA and endogenous H2O2 were decreased. Heat stress and exogenous H2O2 both increased the activities of antioxidant enzymes such as glutathione peroxidase (GSH-Px, EC 1.11.1.9), dehydroascorbate reductase (DHAR, EC 1.8.5.1), monodehydroascorbate reducatase (MDHAR, EC 1.6.5.4), and glutathione reductase (GR, EC 1.6.4.2). The combined effect of heat stress and H2O2 pretreatment led to higher activity of antioxidant enzymes including superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), GSH-Px, DHAR, MDHAR and GR in comparison to the heat treatment alone. We propose that exogenous H2O2 increases antioxidant enzyme activities in cucumber leaves, decreases lipid peroxidation, and thus protects the ultrastructure of chloroplasts under heat stress. 相似文献
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沙棘维生素C积累与相关酶活性关系研究 总被引：1，自引：0，他引：1
探讨沙棘(Hippophae rhamnoides Linn.)不同器官中抗坏血酸(As A)积累与相关代谢酶活性的关系。结果表明,As A在沙棘幼叶和果实中含量较高,在茎和花中含量较低。沙棘幼叶和果实中As A含量主要受L-半乳糖-1,4-内酯脱氢酶(Gal LDH)和脱氢抗坏血酸还原酶(DHAR)活性调节,抗坏血酸氧化酶(AAO)和抗坏血酸过氧化物酶(APX)对As A的积累也有调节作用。 相似文献
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Zhong-Jing Liu Xiao-Long Zhang Ji-Gang Bai Bang-Xia Suo Pei-Lei Xu Lin Wang 《Scientia Horticulturae》2009
In order to examine whether paraquat modifies the functioning of antioxidants and oxidative stress levels in drought-stressed plants, a cucumber cultivar (Cucumis sativus cv. Yuexiu no. 3) was grown hydroponically for 2 days. Drought stress, which was induced by polyethylene glycol (PEG), increased the contents of malonaldehyde (MDA), superoxide radical (O2−) and hydrogen peroxide (H2O2) in cucumber leaves, while pretreatment of paraquat decreased them. Under drought stress induced by PEG, we observed the decreased contents of MDA, H2O2 and O2− in paraquat-pretreated plants in comparison to unpretreated stressed plants. Drought stress and paraquat both increased the activities of antioxidants such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), guaiacol peroxidase (GPX, EC 1.11.1.7), ascorbate peroxidase (APX, EC 1.11.1.11), dehydroascorbate reductase (DHAR, EC 1.8.5.1), monodehydroascorbate reducatase (MDHAR, EC 1.6.5.4), glutathione reductase (GR, EC 1.6.4.2), reduced glutathione (GSH) and reduced ascorbate (AsA). But the combined effect of paraquat application and drought stress resulted in the highest activities of antioxidants. So paraquat is able to moderate the activities of scavenging system enzymes and to influence oxidative stress intensity under drought stress induced by PEG. 相似文献
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El?bieta KielakCezary Sempruch Halina MioduszewskaJózef Klocek Bogumi? Leszczyński 《Pesticide biochemistry and physiology》2011,99(3):237-243
Glyphosate-based herbicides (e.g. Roundup Ultra 360 SL) are extensively used in aquatic environment. Although glyphosate is more environmental favorable than many other herbicides, it may be exceptionally dangerous for aquatic ecosystems through high water solubility. Thus, the aim of the work was quantification of influence of Roundup Ultra 360 SL (containing isopropylamine salt of glyphosate as an active ingredient) on biomass and chlorophyll content within duckweed (Lemna minor L.). Moreover, changes in polyamine content and activity of such antioxidative enzymes as catalase (CAT) and ascorbate peroxidase (APX) were assayed in order to determine the biochemical mechanisms of L. minor response to the herbicide treatment. Obtained results showed that phytotoxicity of the herbicide was connected with decrease in chlorophyll-a, b and a+b content, and reduction of biomass growth. Roundup, similarly to some abiotic and biotic stressors, caused over-accumulation of putrescine, spermidine and total polyamines (PAs) within duckweed tissues. In addition an increase in CAT and APX activities suggested that stress generated by the herbicide treatment was at least partially connected with oxidative burst. Intensity of the duckweed responses to the herbicide was dependent on the applied herbicide level and/or duration of treatment. 相似文献
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