Detect unique molecular structure associated with physiochemical properties in CDC varieties of oat grain with unique nutrient traits [Feed Type vs. Milling Type] in comparison with barley grain using advanced molecular spectroscopy as a non-destructive biological tool     

《Journal of Cereal Science》2017

The objective of this study was to determinate grain unique protein inherent molecular structure that are related physiochemical and nutrient profiles in CDC developed oat varieties [CDC Nasser (Feed Type) and CDC Seabiscuit (Milling Type)] grown in cool climate condition in western Canada in comparison with conventional barley variety of CDC Meredith as a control using advanced molecular spectroscopy. Multivariate analyses, including an agglomerative hierarchical cluster analysis (CLA) and principal component analysis (PCA), were performed to identify protein molecular structural differences among the grains. The results revealed that CDC Seabiscuit contained greater (P < 0.05) protein structural Amide I and II than CDC Nasser and CDC Meredith, while the greater (P < 0.005) structural Amide I to II area and height ratios was detected in CDC Meredith. New oat grains had greater (P < 0.05) β-sheet height than barley grains, however, there was no difference in α-helix to β-sheet ratio values among the varieties. In conclusion, CDC Nasser and CDC Meredith had no difference in protein molecular structural features, while CDC Seabiscuit contains different protein structural characteristics as compared to CDC Meredith grain. The molecular structure features are highly associated with physiochemical and nutrient profiles in grains, which indicate that it also affect nutrient utilization and availability.  相似文献   

Identification of an H1N1 subtype of swine influenza virus and serological analysis     

Fa-chao SUN  Min TAN  Yuan-chao ZHANG  Yu-chao WANG  Sheng-liang CAO  Guo-fei DING  Fang-yuan CONG  Li-hong GUO  Si-dang LIU  Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.  相似文献   

小叶杨基因资源异地保存及苗期生物学特性分析     

付贵生  杨自湘  马金昌  张军红 《林业科技》2005,30(1):1-4

分别从东北、西北和华北收集小叶杨各类资源。并建立了异地保存基因库。同时，对小叶杨资源苗期的生长、物候期、形态特征以及同工酶谱进行了观测和分析。  相似文献   

初冬早春南昌郊区灯下常见蛾类活动情况纪实     

章士美  赵凤霞 《江西农业大学学报》1994,16(1):47-51

  相似文献   

浙农大A系意蜂生产性能考察试验报告   总被引：5，自引：0，他引：5  

沈基楷  肖体元 《中国蜂业》1993,(1):4-6

用杂交一代蜂群进行对比试验的结果表明,浙农大 A 系意蜂的产育力、群势增长速度、采蜜量等,与平湖意蜂和澳大利亚意蜂无显著差异或基本一致,但蜂王浆高产性能表现突出,产量比平湖意蜂高19.5%,比澳意高42.5%,而且质量指标符合国家规定和出口标准。  相似文献   

新疆农田土壤有机质含量及组成特征   总被引：3，自引：0，他引：3  

姜益娟  郑德明  吕双庆  朱朝阳  伍维模  柳维杨 《干旱地区农业研究》2003,21(4):61-64

目前新疆农田土壤有机质含量在5．30～24．21g／kg之间，平均14．65g／kg，与第二次土壤普查相比，新疆农田土壤有机质含量呈上升趋势；胡敏素碳含量占到有机质碳量的53％～70％，平均61％，腐殖酸碳量只占39％。  相似文献   

二丁酰环腺苷酸对猪胴体组成和肉品质的影响   总被引：1，自引：0，他引：1  

张曦  高士争  程美玲  刘勇  严达伟 《中国畜牧杂志》2004,40(5):20-22

通过皮下注射和饲料添加不同剂量dbcAMP进行饲养试验 ,研究其对肥育猪胴体组成和肉品质的影响。结果表明 :皮下注射 1.0mg/kg和饲料添加 2 0mg/kg对改善肥育猪的胴体组成和肉品质效果最好 ,眼肌面积分别提高31.99%和 36 .39% (P <0 .0 5 ) ;瘦肉率分别提高 12 .4 2 %和 11.94 % (P <0 .0 5 ) ;背膘厚分别降低 19.95 %和 2 0 .97%(P <0 .0 5 )。肌肉粗蛋白含量分别提高 10 .87%和 16 .5 4 % (P <0 .0 5 )。  相似文献   

广西5种人工林地土壤微生物数量及土壤酶活性分析     

王冠玉  吕世凡  吕成群  梁朝旭  李鸣 《河北农业大学学报》2017,40(1)

为当地珍贵速生树种人工林的发展及合理利用提供理论依据。分析广西5种珍贵树种人工林土壤微生物数量及土壤酶活性差异,以相同立地条件下12年生的黑木相思(Acacia melanoxylon)、老排(Mytilaria laosensis)、红椎(Castanopsis hystrix)、黧蒴椎(Castanopsis fissa)和火力楠(Michelia macelurei)人工纯林为研究对象,采用稀释平板涂布法和土壤酶活性测定法,测定和分析该5种人工林地土壤微生物数量及酶活性。结果表明:5种林地的土壤微生物总数和细菌数量大小均为:火力楠黧蒴锥红椎黑木相思米老排。但5种林地的土壤酶活性大小不一,蔗糖酶、脲酶、蛋白酶、酸性磷酸酶活性在5种林地之间的差异极其显著,而过氧化氢酶活性不显著。5种林地中土壤酶活性相对较强的是米老排人工林,较弱的是黑木相思林和火力楠林。土壤酶活性与微生物数量的相关分析表明,土壤中蔗糖酶活性与放线菌数量呈极显著正相关关系,其余相关性没有达到显著水平。  相似文献   

国土空间详细规划现状底图底数转换方法研究     

闫保银  石林曦  管佳 《现代农业科技》2023,(18)

统一底图底数是编制各类空间规划的重要前提和基础,第三次国土调查数据作为当前权威性最高、现势性最强的国土空间利用现状数据,所采用的工作分类与国土空间规划采用的用地用海分类存在较大差异。本文通过对工作分类和用地用海分类进行衔接分析,提出了基于第三次国土调查数据的国土空间详细规划现状底图底数转换的总体技术思路、关键技术方法。  相似文献   

湘西北长果安息香种群数量与分布格局     

张程 《湖南农业大学学报(自然科学版)》2011,37(6):615-620

对湖南石门壶瓶山景关门、桑植龙潭坪茅垭村样地的长果安息香(Changiostyrax raxdolichocarpa)种群数量和分布格局进行调查和分析。结果表明：长果安息香种群的年龄结构呈衰退型，个体数量主要集中在小树(IV龄段)，幼龄级株数和老龄级株数匮乏；长果安息香种群在进入生理死亡年龄前有2个死亡高峰，第1个峰值产生于幼树(II、III龄段)，主要原因是种内和种间竞争(主要是光照)，第2个峰值产生于中树长成大树阶段(V、VI龄段)，除种内、种间对环境的竞争外，生长地土层浅薄、大树倒伏及人为砍伐亦加剧了该峰值的产生；种群整体呈聚集分布类型，小树向中树、大树变化过程表现出聚集分布向随机分布转变，导致长果安息香濒危的主要原因是种子萌发率极低、种群竞争能力较差以及人为干扰等。  相似文献