绢丝昆虫茧丝蛋白酶抑制剂含量的比较分析   总被引：3，自引：3，他引：0  

裘智勇  张志芳  赵巧玲  肖庆利  叶夏裕  何家禄 《蚕业科学》2001,27(2):109-113

以 6种绢丝昆虫的茧壳为材料 ,对茧丝蛋白酶抑制剂的含量及热稳定性进行了研究。结果表明 :家蚕品种间茧丝蛋白酶抑制剂的含量差异极显著 ,外层丝中含量较多 ,F1代的含量呈双亲中间型 ,偏向于含量较多的亲本 ,雌雄间含量无显著差异 ;家蚕茧丝蛋白酶抑制剂对热稳定性较强 ;天蚕绿茧、野蚕的茧丝中有少量的蛋白酶抑制剂存在 ,蓖麻蚕的茧丝中有微量蛋白酶抑制剂存在 ,天蚕黄茧、柞蚕茧、樗蚕茧的茧丝中基本无蛋白酶抑制剂存在。  相似文献   

花粉中酶的研究   总被引：3，自引：0，他引：3  

王开发  张玉兰 《蜜蜂杂志》1997,(9):3-5

花粉中酶的研究王开发张玉兰张盛隆（同济大学花粉应用研究中心）酶是影响细胞代谢的重要物质，是摄入生物体内的营养成分进行分解、合成时起催化作用，而称为生物催化剂。但酶与其它催化剂不同，能在机体十分温和的条件下高效率地起催化作用，使生物体内的各种物质处于不...  相似文献   

银杏幼苗叶片转化酶活性及其动力学   总被引：4，自引：0，他引：4  

苏冬梅  罗丽华 《经济林研究》2004,22(4):10-11,18

采用波钦诺克XH法研究了田间和室内栽培的银杏幼苗叶片转化酶的活性及其动力学性质。结果表明:银杏幼苗叶片转化酶在pH值5.0、温度55℃时活性最强;该酶的米氏常数为3.7×10-2mol·L-1;室内盆栽的银杏幼苗叶片转化酶的活性显著高于田间栽培的同龄银杏幼苗叶片转化酶的活性。  相似文献   

Enantioselective degradation kinetics of metalaxyl in rabbits     

Jing Qiu 《Pesticide biochemistry and physiology》2005,83(1):1-8

Metalaxyl [methyl-N-(2′-methoxyacetyl)-N-(2,6-dimethylphenyl)-d,l- alaninate] is a potent phenylamide fungicide. The (−)-(R)-isomer accounts for most of the fungicidal activity. A possible stereo and/or enantioselective kinetics of metalaxyl in rabbits was investigated by intravenous injection. The concentrations of (−)-(R)- and (+)-(S)-metalaxyl in plasma, liver, and kidney tissue were determined by HPLC with a cellulose-Tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase and gas chromatography-mass spectroscopy. After intravenous administration of racemic metalaxyl (40 mg/kg), the (+)-(S)-enantiomer levels in plasma, liver, and kidney decreased more rapidly than the (−)-(R)-isomer. The area ratio of the (−)-(R)-/(+)-(S)-enantiomer under the concentration-time curve (AUC_0 → ∞) in plasma after drug application was 1.62. The total plasma clearance value of the (+)-(S)-enantiomer was 1.53 and higher than that of the (−)-(R)-enantiomer. The [R]/[S] ratio in plasma was >1 for standard rac-metalaxyl at each time point. The other pharmacokinetic parameters of the enantiomers were also different. The results indicate substantial stereoselectivity in the degradation of metalaxyl enantiomers in rabbits.  相似文献   

The effects of azadirachtin and nucleopolyhedrovirus on midgut enzymatic profile of Spodoptera litura Fab. (Lepidoptera: Noctuidae)     

S. Senthil Nathan  K. Kalaivani 《Pesticide biochemistry and physiology》2005,83(1):46-57

The effects of azadirachtin (AZA) and nucleopolyhedrovirus (NPV) on midgut enzyme activity in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) (Tobacco cutworm) were evaluated. Gut enzyme activities were decreased by AZA and NPV individually and in combination. When S. litura larvae were fed a diet of castor leaves treated with AZA and NPV in bioassays, gut enzyme—acid phosphatases, alkaline phosphatases, adenosine triphosphatases, and lactate dehydrogenase—activities were decreased. There were statistically significant differences (P ? 0.05) in enzyme activities between combined and individual treatment. A synergistic effect of botanical insecticides and virus was found when combined in low doses. These effects are most pronounced in early instars. Maximum weight loss (59-72%) occurred, when AZA and NPV were combined.  相似文献   

茭白肉质茎膨大过程中的糖代谢与激素含量变化   总被引：8，自引：1，他引：8  

江解增  曹碚生  黄凯丰  张强  韩秀芹  朱庆森 《园艺学报》2005,32(1):134-138

 以单季茭白品种‘蒋墅茭’和双季茭白品种‘葑红早’为试材, 研究了秋季肉质茎膨大过程中物质运转的相关生理生化变化。结果表明, 随着肉质茎鲜质量、干质量快速增加, 非结构性碳水化合物主要成分含量总体上升, 后期出现下降, 蒋墅茭肉质茎中蔗糖含量较高; 糖分转化关键酶活性总体较高,转化酶活性以酸性转化酶较高且呈快速下降, 蒋墅茭的酸性转化酶活性高于葑红早而中性转化酶活性较低;蔗糖合成酶的分解方向活性显著高于合成方向, 且后期维持较高水平, 葑红早的双向活性均高于蒋墅茭;Z + ZR总体含量最高、下降最明显, GA₄ 其次, IAA也呈缓慢下降趋势, GA₃、ABA含量极低, 且各激素均以葑红早高于蒋墅茭。茭白肉质茎干、鲜质量处于快速增长阶段的同时, 而内部生理生化快速下降, 说明茭白肉质茎膨大过程具有特殊性, 并且品种间存在差异。  相似文献   

红星苹果和肥城桃果实软化的酶学基础     

李富军  张新华  翟衡 《果树学报》2005,22(5):450-453

以红星苹果穴Malusdomesticacv.Starking雪和肥城桃穴Prunuspersicacv.Feicheng雪果实为试材,结合乙烯利和1-甲基环丙烯(1-MCP)处理,分别研究了2者软化的酶学基础,并就其差别作了探讨。结果表明:红星果实软化中起主要作用的酶为内切和外切多聚半乳糖醛酸酶(endo-和exo-PG);肥城桃果实软化的前20d则以纤维素酶(EGase)为主,20d后软化以endo-PG为主。研究同时发现,脂氧合酶(LOX)在这2种果实软化的启动阶段都起了明显的促进作用。1-MCP通过对红星果实中PG、肥城桃果实贮藏前期EGase和后期endo-PG活性的抑制,以及对2者软化启动阶段LOX活性的抑制,延缓了果实硬度的下降。  相似文献   

不同含水量菜豆种子老化过程中生理特性的研究   总被引：2，自引：0，他引：2  

李玉红  陈鹏  唐爱均  王绮  关志华  程智慧 《园艺学报》2005,32(5):908-910

 对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。  相似文献   

Comparative studies of acetylcholinesterases purified from two field populations of the oriental migratory locust (Locusta migratoria manilensis): implications of insecticide resistance     

En-Bo Ma  Kun Yan Zhu 《Pesticide biochemistry and physiology》2004,78(1):67-77

Acetylcholinesterase (AChE) was purified by affinity chromatography from two populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from Huanghua and Pingshan Counties, Hebei Province of China. The purification factors and yields were 1661-fold and 19.3%, respectively, for the Huanghua population, and 3897-fold and 39.6% for the Pingshan population. Both the purification factor and yield were significantly lower in the Huanghua population than in the Pingshan population. AChE activity was almost completely inhibited by 10⁻⁶ M eserine and BW284C51, but ?5.8% of AChE activity was inhibited by ethopropazine at the same concentration, suggesting that purified AChE from either population was a typical insect AChE. However, AChE purified from the Huanghua population was 62-, 2.0-, and 1.6-fold less sensitive to inhibition by the three organophosphate compounds, chlorpyrifos oxon, demeton-S-methyl, and paraoxon, respectively, than that from the Pingshan population. Significantly lower purification factor and low yield associated with reduced sensitivity of AChE to inhibition by the organophosphates indicated that AChE purified from the Huanghua population was biochemically and pharmacologically different from that of the Pingshan population. Reduced sensitivity of AChE appeared to contribute to organophosphate resistance in the locust from Huanghua County, where insecticides have commonly been used to manage outbreaks of the locust.  相似文献   

山羊生精上皮细胞分离研究   总被引：1，自引：0，他引：1  

杨延飞  李学佳  王勃森  肖勇  张涌 《黑龙江畜牧兽医》2005,(2):5-7

采用四种酶法分离二月龄关中奶山羊生精上皮细胞,A组:胶原酶Ⅳ;B组:胶原酶Ⅳ +透明质酸酶;C组:胶原酶Ⅳ+透明质酸酶+胰蛋白酶与EDTA;D组:胶原酶Ⅳ+透明质酸酶+胰蛋白酶与EDTA+DnaseI。结果二月龄羔羊曲细精管主要包含Sertoli细胞、精原细胞和管周排列的肌样细胞。每 200mg睾丸实质收获生精上皮细胞总数 (×105 )A,B,C,D组分别为 6. 91±0. 68, 6. 67±0. 80, 5. 94±0. 81, 5. 74±0. 82;细胞存活率 (% )分别为 76. 82±3. 80, 75. 96±1. 61, 94. 19±2. 89, 92. 32±1. 97;圆形细胞比率 (% )分别为 17. 54±1. 68, 16. 70±1. 46, 23. 64±1. 72, 22. 90±2. 38;细胞贴壁率(% )分别为 5. 93±0. 36, 5. 81±0. 54, 26. 94±1. 54, 25. 00±1. 74。C,D组不但组织块解离效果及细胞离散程度均较A,B好,且原代培养细胞团块数少。多重比较C,D组显著优于A,B组 (P<0.05);C组与D组差异不显著(P>0.05),但与A,B组比较细胞存活率、圆形细胞比率及贴壁率差异极显著(P<0.01)。结论认为C组是较简单有效的山羊生精上皮细胞分离酶组合。  相似文献