母猪诱导发情技术     

王自恒  林峰 《畜牧兽医杂志》2004,23(1):40-41,43

本文就母猪各种诱导发情方法的原理及应用现状进行了概述。认为诱导排卵技术是解决母猪生理性乏情的根本途径。  相似文献   

β- 氨基丁酸诱导甜( 辣) 椒抗疫病作用的研究   总被引：12，自引：2，他引：12  

谢丙炎  李惠霞  冯兰香 《园艺学报》2002,29(2):137-140

 报道了用β- 氨基丁酸( DL-β-aminon-butyric acid, BABA) 喷雾处理辣椒叶片和茎后的诱导抗疫病作用。研究证明: 高浓度BABA ( 1 000 g/mL) 对离体辣椒疫霉病菌无抗菌活性, 用其喷雾处理辣椒的茎叶所诱导的抗疫病作用可完全控制其危害; 用BABA 诱导处理后3 d 接种辣椒疫霉病菌, 辣椒植株开始表达出较高的诱导抗性, 这种抗病作用可持续20 d 以上, 并表现出与数量抗病性相似的特性。  相似文献   

β-氨基丁酸、茉莉酸及其甲酯诱导辣椒抗TMV作用的研究   总被引：5，自引：2，他引：5  

梁俊峰  谢丙炎  张宝玺  冯兰香 《中国蔬菜》2003,1(3):4-7

β -氨基丁酸 (BABA)、茉莉酸 (JA)及其甲酯 (MeJA)喷雾处理辣椒叶片和茎秆后可诱导辣椒获得对TMV的抗性。研究结果表明 :3种诱抗剂对不同辣椒品种所表现的诱抗效果没有显著差异 ;诱抗效果受诱抗剂的使用浓度、作物的生育期和TMV的接种浓度所影响 ;最高的诱抗效果可达到 5 0 %以上 ;BABA处理后 5～ 6d ,JA与MeJA处理后 6～ 10d接种TMV ,辣椒植株开始表达出较高的诱导抗性 ,这种抗性可持续 15d以上 ,并表现出与数量抗病性相似的特性。  相似文献   

Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells     

LI Fu-hai  LI Zong-zhuang  JIANG Zhi  YI Wei  ZHANG Chen-yun 《园艺学报》2015,31(6):1130-1136

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.  相似文献   

农业技术模式转换与对策探讨——以河南省欠发达平原农业区为例   总被引：1，自引：0，他引：1  

邵明伟 《河南农业科学》2011,40(7):1-4

面对当前农村劳动力转移给农业发展带来的挑战和机遇,转换农业技术模式尤为重要.采用理论分析和调查访谈相结合的方法,得出河南欠发达平原农业区农业技术模式转换必须结合本地实际,遵循诱致性轨迹,注重转换过渡模式,即以农民社会资本为纽带进行土地流转,形成土地适度渐进规模经营,以村集体、宗族等为核心发展种子、生物科技、中小型机械设...  相似文献   

Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss     

Arya Vazirzadeh  Abdolmajid Hajimoradloo  Hamid Reza Esmaeili  Mostafa Akhlaghi 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):267-269

The effects of saline-dissolved or Freund's incomplete adjuvant (FIA)-emulsified GnRHa treatment on the induction of ovulation in rainbow trout, Oncorhynchus mykiss, were examined. The FIA-emulsified GnRHa was first diluted in 0.25 ml physiological saline and then mixed with an equal volume of FIA. Fish were selected in the beginning of the spawning season and were allocated into four groups and were treated intraperitoneally with (a) 0.5 ml of emulsified GnRHa (GnRHa–FIA), (b) 0.5 ml saline-dissolved GnRHa in a single injection (GnRHa-1), (c) 0.5 ml saline-dissolved GnRHa in two injections spaced 1 d apart (GnRHa-2) and (d) 0.5 ml of saline (Control). The GnRHa dose in all hormone treatments was 25 µg kg^− 1. All fish in the FIA–GnRHa and GnRHa-2 groups ovulated within 10 and 11 d after treatment, respectively. In contrast, only 75% in the Control fish and 60% of the fish in the GnRHa-1 group ovulated within 36 d after treatment. None of the treatments caused any pre- or post-spawning mortality in the broodstock. Fertilization, eyeing and hatching percentages of the produced progeny were normal in all the treatment groups and did not differ significantly among them. In conclusion, FIA-emulsified GnRHa can be effective in advancing the onset of and synchronizing the ovulation of rainbow trout within a two-week period, thus shortening the egg collection period, without affecting broodstock survival and egg quality.  相似文献   

虹鳟排卵前后血清中性类固醇激素浓度变化的研究   总被引：4，自引：0，他引：4  

赵维信 《水产学报》1987,11(3):205-213

对虹鳟(Salmo gairdneri)排卵前后血清17β-雌二醇(17β-E_2),睾酮(T)和17α-羟-20β-双氢孕酮(17α20βP)的含量进行了测定。17β-E_2从排卵前15天的18.1ng/ml急骤下降到排卵前3—6天的2ng/ml,并继续下降,至排卵时仅为0.9ng/ml。睾酮在虹鳟雌鱼血清中的含量很高,血清浓度从排卵前9天的峰值水平143.3ng/ml缓慢下降,排卵时为24.3ng/ml。17α20βP的血清浓度变化明显,排卵前15天,该激素浓度接近于基线水平或甚至低得不能被检测,排卵前9天开始迅速上升,到排卵前3天达到峰值350.6ng/ml,排卵时为302.2ng/ml。本研究进一步证实了17α20βP在鲑鳟鱼类卵母细胞最后成熟过程中的生理作用,是一种诱发卵母细胞成熟的类固醇激素。临排卵前,血清17β-E_2浓度的下降,可能调节了17α20βP大量分泌的时间;17α20βP的大量分泌是卵母细胞达到最后成熟和排卵不可缺少的一环。注射合成的大麻哈鱼(Oncorhynchus keta)促性腺激素释放激素(s-GnRH)及其类似物(s-GnRH-A),诱发虹鳟血清类固醇激素17β-E_2,睾酮和17α20βP的变化趋势与自然排卵时的变化相类似。经注射药物诱发排卵的鱼,较对照组提早一周排卵,而且排卵较同步和集中。  相似文献   

尖头金线鲃人工繁殖初步研究   总被引：3，自引：0，他引：3  

严晖  高慧  李德运  董文红  余春  余云仙 《水生态学杂志》2009,30(4):143-145

用人绒毛膜促性腺激素（HCG）、促黄体素释放激素类似物（LRH—A2）、马来酸地欧酮（DOM）组合对尖头金线鲃（Sinocyclocheilus oxycephalus）催产获得成功,用鲤脑垂体5mg／kg和人绒毛膜促性腺激素1000IU／kg组合催产失败。采取2次注射（第1次注射LRH—A2 2μg／kg,第2次注射LRH—A2 8μg/kg＋DOM3mg／kg＋HCG 1000IU／kg）催产效果明显;采取遮光孵化,孵化率明显提高。  相似文献   

高活性促性腺激素类似物及其复配制剂在鱼类人工繁殖中的应用     

宋艳 《水产科技情报》1999,26(1):16-20

通过催产试验,在高活性促性腺激素类似物相互之间、在类似物与ＨＣＧ之间、在类似物同ＨＣＧ或ＨＷＨＯ复配而成的复配制剂相互之间、在类似物与其复配制剂之间,进行了催产效果的对比。结果表明,各种类似物对不同鱼类的催产效果各不相同;类似物的催产效果明显优于ＨＣＧ;使用类似物复配制剂,其催产效果优于单独使用ＨＣＧ或类似物。  相似文献   

胚胎干细胞的研究进展及应用前景     

程三宝 《畜禽业》2007,(6):14-17

干细胞(Stem Cell)是一类具有分化潜能和自我复制的早期未分化细胞。胚胎干细胞(Embryonic stem cells,ES细胞)是一种早期胚胎内细胞团(inner cell mass,ICM)或原始生殖细胞(primordial germ cell,PGC)经体外分化抑制培养,分离和克隆得到的具有发育全能性的高度未分化细胞。本文综述了胚胎干细胞的形态学、生长特性、免疫学鉴定方法,ES细胞抑制分化和诱导分化的机理以及饲养层、血清和细胞因子等影响胚胎干细胞分离克隆的因素,并进一步阐述了胚胎干细胞的应用前景以及存在的问题。  相似文献