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1.
《Journal of Cereal Science》2014,59(3):451-456
Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities in vitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes. 相似文献
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The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry 相似文献
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Several parameters related to cold damage in zucchini have been measured in fruits from different varieties cultivated in the province of Almería (south-eastern Spain). The purpose of this work is to correlate physiological parameters with commercial quality and chilling damage, in order to establish reliable criteria for selecting varieties having better genetic adaptation to low-temperature storage. The results show that, for varieties harvested during February, the variety Natura was the best adapted to storage at 4 °C, as its fruits suffered less from chilling injuries, had a lower weight loss, and the levels of metabolites malonyldialdehyde and H2O2 were not as high as in the other varieties. Genetic variation was detected for some of the parameters analysed. The positive correlation among the levels of MDA, H2O2, and chilling injury, as well as the negative correlation among catalase and chilling injury, makes these parameters good indicators of chilling damage in our system. 相似文献
4.
分别用常规氯氨T和改进的双相氯氨T法对PEG-rhIL-6进行放射性碘标记,用柱层析法和离心超滤法对标记物分离纯化,同时用三氯乙酸沉淀法和SDS-PAGE电泳法鉴定纯化后的标记化合物放射化学纯度,采用rhIL-6依赖细胞7TD1,用MTT比色法测定标记物的生物学活性。结果表明:1.改进的双相氯氨T法标记率为74.5%,高于常规氯氨T法的62.3%,放射性比活度分别为5.513×105和4.610×105Bq/μg。2.经柱层析法和离心超滤法对标记物分离纯化后,用三氯乙酸沉淀法测得放射化学纯度均能达到99%以上。3.用SDS-PAGE电泳法检测两种标记方法所得的标记物与非标记物的结果表明,常规氯氨T法标记物比非标记物多1条高分子量蛋白带,认为是标记过程中蛋白质受到部分损伤;改进的双相氯氨T的标记物与非标记物蛋白质条带一致,认为蛋白质标记后基本没有受到损伤。4.生物活性检测表明改进的双相氯氨T标记物与非标记物活性之间无显著差异,常规氯氨T法标记物活性略低于双相氯氨T的标记物活性。 相似文献
5.
Mythily SubramaneyaanSachin Rustagi Sambit N. BhattacharyaAsok K. Tripathi Basu D. BanerjeeRafat S. Ahmed 《Pesticide biochemistry and physiology》2012,102(1):91-94
The scabicide, lindane induces oxidative stress and immunological alterations. The present study was undertaken to assess the ameliorative effects of antioxidant supplementation in lindane treated scabies patients. Scabies patients were treated with either 1% lindane or 1% lindane along with antioxidant (Lycored or Vitamin-E). Oxidative stress and immunological parameters were evaluated in blood samples and compared with healthy controls. Lindane caused a significant increase in malonedialdehyde (MDA) levels and decrease in reduced glutathione (GSH) and ferric reducing ability of plasma (FRAP), which was attenuated by anti-oxidant therapy. The IL-1α levels were significantly enhanced in scabies patients per se and remained unaffected after lindane/anti-oxidant treatment. The TNF-α and nitroblue tetrazolium (NBT) reduction levels were not significantly different in all the groups. Topical application of lindane induces significant free radical generation and may cause immunological alterations which can be reversed by antioxidant therapy. 相似文献
6.
Wheat yield and quality are dependent largely on nitrogen (N) availability. In this study, we performed the first metabolomic analysis of the response to high-N fertilizer during wheat grain development using non-targeted gas chromatography-mass spectrometry (GC–MS). Quality parameter analyses demonstrated that high-N fertilizer application led to a significant increase in grain protein content and improvement in starch and bread-making quality. Comparative metabolomic profiling of six grain developmental stages resulted in identification of 74 metabolites, including amino acids, carbohydrates, organic acids and lipids/alcohol, which are primarily involved in carbon and N metabolism. Under high-N fertilizer treatment, numerous metabolites accumulated significantly during grain development. Principal component analysis revealed two principal components as being responsible for the variances resulting from N-fertilizer treatments. Metabolite–metabolite correlation analysis demonstrated that the high-N treatment group had a greater number of positive correlations among metabolites, suggesting that high-N fertilizer treatment induced a concerted metabolic change that resulted in improved grain development. Particularly, the high-N treatment-mediated significant accumulation of metabolites involved in the TCA cycle, starch and storage protein synthesis could be responsible for the improvement of grain yield and quality. Our results provide new insight into the molecular mechanisms of wheat grain development and yield and quality. 相似文献
7.
条斑紫菜多糖脱蛋白方法与条件优化 总被引：1，自引：0，他引：1
对紫菜多糖提取液中的杂蛋白质去除方法和条件优化进行了研究。采用Sevag法与三氯乙酸（TCA）法去除紫菜多糖中的游离蛋白质,分别研究了不同溶剂体积比、用量、处理温度和时间等主要因子对蛋白质去除的影响。结果表明,Sevag法最佳脱蛋白条件为氯仿：正丁醇=3：1,样品：氯仿-正丁醇=3：1,振荡时间为30min;三氯乙酸法（TCA法）最佳脱蛋白条件为反应温度80℃、三氯乙酸用量4％、反应时间30min。两种脱蛋白方法比较发现：三氯乙酸法去除蛋白质质的效果优于Sevag法。 相似文献
8.
长效促红细胞生成素的放射性标记 总被引：1，自引：1，他引：1
采用改进的双相氯胺 T法对LL EPO进行碘标记 ,使用离心超滤法分离纯化标记混合物 ,对标记纯化的LL EPO经三氯乙酸沉淀和SDS PAGE法鉴定放化纯度 ,通过网织红细胞法对标记前后的蛋白生物活性进行鉴定。结果表明 :1 改进的双相氯胺 T法标记LL EPO ,其标记率为 89% ,比放射性为 5 82× 1 0 5Bq·μg-1蛋白 ,放化纯度 >96% ,SDS PAGE法鉴定标记产物同原型蛋白电泳行为一致 ,Rf值分别为 0 2 8、0 49,网织红细胞法鉴定的标记蛋白与非标记蛋白的生物活性无差异。2 离心超滤法得到的蛋白回收率为 95 %以上 ,而凝胶过滤法得到的蛋白回收率仅为 2 3 82 % ;前者得到的标记蛋白浓度远高于后者 相似文献
9.
Mediha Sefi Hanen Bouaziz Nejla Soudani Tahia Boudawara Najiba Zeghal 《Pesticide biochemistry and physiology》2011,101(2):71-79
Fenthion (FEN) is an organophosphate insecticide used in both agricultural and urban areas throughout the world including Tunisia. Recent investigations have proved the crucial role of natural antioxidants to prevent the damage caused by toxic compounds. In this study, we investigated the role of Artemisia campestris (Ac) leaf powder in protection against oxidative damage and hepatotoxicity induced by fenthion in female rats and their pups. Female Wistar rats were divided into four groups: group I served as controls which received standard diet, group II received orally FEN 551 ppm, group III received both 551 ppm of FEN and experimental diet (5% Artemisia) and group IV received experimental diet (5% Artemisia). Oral administration 551 ppm of FEN by drinking water to adult rats caused hepatotoxicity as monitored by the increase in the levels of hepatic markers enzymes (transaminases and lactate dehydrogenase), total cholesterol (TC) and triglycerides (TG), as well as hepatic malondialdehyde (MDA) levels thus causing a drastic alteration in antioxidant defence system. Particularly, the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the level of reduced glutathione (GSH) increased by FEN. These biochemical alterations were accompanied by histological changes marked by leucocytes infiltration, sinusoidal dilatation (moderate peliosis), granuloma inflammatory disorders and necrosis in hepatocytes of dams. While, slight leucocytes infiltration was shown in pups. Treatment with Ac prevented the liver damage induced by FEN, as revealed by inhibition of hepatic lipid peroxidation accompanied by an improvement of liver histopathological changes, CAT and GPx activities except GSH and SOD which were not modified. It could be concluded that A. campestris is promising a protective agent against hepatotoxicity during the exposure to fenthion. 相似文献
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