A releaser pheromone that attracts methyltestosterone- treated immature fish in the urine of ovulated female rainbow trout     

Hidenobu Yambe  Fumio Yamazaki 《Fisheries Science》2001,67(2):214-220

SUMMARY: Behavioral experiments concerning a releaser pheromone in the urine of female rainbow trout were performed using immature fish administered orally with 17α-methyltestosterone (MT) during the non-spawning season. The urine was collected by catheter. The frequency of entries of test fish was recorded in each channel scented by test and control solutions in a Y-maze trough. The behavior of both MT-treated and control fish demonstrated that they could not discriminate the differences between distilled and environmental water as control solutions. There was also no difference between MT-treated and control fish when distilled and environmental water were introduced. The MT-treated immature fish were attracted to the channel scented by ovulated female urine. Neither coelomic fluid nor the immature female urine had any effect on the behavioral responses of MT-treated fish, while immature control fish had no preference for the urine of ovulated females. These results suggest in rainbow trout that ovulated female urine contains a releaser pheromone to attract mature males, and that androgens are involved in the sensory mechanisms detecting the releaser pheromone in fish.  相似文献   

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR-124a     

Xinpeng Yang  Yue Feng  Yang Li  Dake Chen  Xuanyan Xia  Jialian Li  Fenge Li 《Reproduction in domestic animals》2021,56(3):416-426

Sertoli cells are the only somatic cells in the seminiferous epithelium which directly contact with germ cells. Sertoli cells exhibit polarized alignment at the basal membrane of seminiferous tubules to maintain the microenvironment for growth and development of germ cells, and therefore play a crucial role in spermatogenesis. Androgens exert their action through androgen receptor (AR) and AR signalling in the testis is essential for maintenance of spermatogonial numbers, blood–testis barrier integrity, completion of meiosis, adhesion of spermatids and spermiation. In the present study, we demonstrated that AR gene could promote the proliferation of immature porcine Sertoli cells (ST cells) and the cell cycle procession, and accelerate the transition from G1 phase into S phase in ST cells. Meanwhile, miR-124a could affect the proliferation and cell cycle procession of ST cells by targeting 3′-UTR of AR gene. Furthermore, AR bound to the RNF4 via AR DNA-binding domain (DBD) and we verified that RNF4 was necessary for AR to regulate the growth of ST cells. Above all, this study suggests that AR regulates ST cell growth via binding to RNF4 and miR-124a, which may help us to further understand the function of AR in spermatogenesis.  相似文献   

Adrenocorticotropic hormone-induced secretion of cortisol in goats is inhibited by androgen     

Yuko MAEJIMA  Masato AOYAMA  Noriko KOBAYASHI  Shoei SUGITA 《Animal Science Journal》2006,77(1):87-94

In a previous study, it was found that there are sex differences in goats with respect to the levels of cortisol secretion induced by transportation stress. We also found that treatment of castrated male goats with dihydrotestosterone (DHT) suppressed the increase in plasma cortisol concentration following transportation, but did not suppress the secretion of adrenocorticotropic hormone (ACTH). This suggests that androgen might block ACTH ‐ induced cortisol secretion. In order to examine this hypothesis, the effects of androgen on ACTH‐induced cortisol secretion in goats were investigated. First, castrated male goats were treated with testosterone (T), DHT or cholesterol (cho) for 21–25 days. Cho was used as a control for T and DHT treatment. Then, plasma cortisol concentrations were compared among the hormonal treatments after ACTH injection. Subsequently, the distribution of androgen receptors in the caprine adrenal gland was investigated. There were no differences in the basal cortisol concentrations among the hormonal treatments. However, plasma cortisol concentrations after ACTH injection were significantly lower in T ‐ and DHT ‐ treated goats than in cho ‐ treated goats. Androgen receptors were present in 60% of the cells in the zonae fasciculata and reticularis of the adrenal cortex, the regions that secrete glucocorticoids. These results suggest that androgen may act directly on the adrenal cortex to suppress cortisol secretion induced by ACTH.  相似文献   

中华鳖脾脏雄激素受体mRNA 的原位杂交检测   总被引：1，自引：0，他引：1  

李梅英  张莉  包慧君  张晖  柳金雄  陈秋生 《中国水产科学》2008,15(2):337-341

采用原位杂交技术（In situ hybridization，ISH）研究雄激素受体（Androgen receptor，AR）mRNA在中华鳖（Pelodiseus Sinensis）脾脏的存在及细胞定位。取5只健康成年中华鳖的脾脏，进行石蜡切片。用地高辛标记的寡核苷酸探针对组织切片进行原位杂交（In situ hybridization，ISH），检测雄激素受体mRNA在脾脏免疫细胞内的表达定位。结果显示，大部分雄激素受体mRNA阳性细胞呈圆形或椭圆形，数量少胞体大且呈不规则形。阳性细胞在脾脏动脉周围淋巴鞘（Pefiatefial lymphatic sheath，PALS）和红髓（Red pulp，RP）内分布密集。椭球周围淋巴鞘（Periellipsoidal lymphatic sheath，PELS）内AR mRNA阳性细胞分布极少。杂交阳性信号物质在细胞质和细胞核均有分布。在标记为阳性的中华鳖脾脏中，推测圆形的阳性细胞可能为B淋巴细胞，而另一部分胞体大且形状不规则的阳性细胞则可能为巨噬细胞。脾脏内AR mRNA的存在进一步证实雄激素可能是调节脾脏等免疫器官功能的因素之一。[中国水产科学，2008，15（2）：337—341]  相似文献   

Pubertal development of male African catfish,Clarias gariepinus. In vitro steroidogenesis by testis and interrenal tissue and plasma levels of sexual steroids     

J. E. B. Cavaco  J. G. D. Lambert  R. W. Schulz  H. J. Th. Goos 《Fish physiology and biochemistry》1997,16(2):129-138

In fish, sex steroids initiate and/or accelerate the maturation of the brain-pituitary-gonad axis. In order to obtain information on the steroid milieu during the pubertal development of male African catfish, we have monitored the conversion of [³H]-pregnenolone and [³H]-androstenedione by testis and [³H]-pregnenolone by interrenal tissue fragmentsin vitro. Pubertal development occurs between two and six months of age. Testicular development proceeds through four main stages that are characterised histologically by the presence of spermatogonia (stage I), spermatogonia and spermatocytes (stage II), spermatogonia, spermatocytes and spermatids (stage III), and all germ cells including spermatozoa (stage IV). 11β-Hydroxyandrostenedione and cortisol were the main products of testes and interrenal tissue, respectively, in all stages of the pubertal development; a change in the steroidogenic pattern was not observed during this period. The interrenal tissue displayed no significant conversion of [³H]-pregnenolone to 11-oxygenated androgens. Blood plasma was analyzed for the presence of five androgens; testosterone, 11β-hydroxytestosterone, 11β-hydroxyandrostenedione, androstenetrione, and 11-ketotestosterone. 11-Ketotestosterone was the quantitatively dominating androgen in the circulation at all stages of development, which was more pronounced in stages III and IV. The obvious differences between thein vitro andin vivo results, namely 11β-hydroxyandrostenedione being the main testicular productvs. 11-ketotestosterone dominating in the blood, may indicate that 11β-hydroxyandrostenedione is converted to 11-ketotestosterone at extratesticular sites.  相似文献   

Isolation of pituitary glycoprotein gonadotropins from the grass carp (Ctenopharyngodon idell)     

J. Y. L. Yu  S. T. Shen 《Fish physiology and biochemistry》1989,7(1-6):177-183

Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were isolated of MW 56,000 and 53,000 daltons, respectively, as determined by gel filtration technique. Isoelectric points of the two GTHs also differed from each other. Sialic acid contents were 3.64% and 1.47% for DE I and DE II, respectively. Both GTH's stimulated, in a similar manner, testicular androgen and ovarian estradiol synthesisin vitro.  相似文献   

Immunohistochemical characterization of canine prostatic carcinoma and correlation with castration status and castration time   总被引：2，自引：0，他引：2  

K. U. Sorenmo  M. Goldschmidt  F. Shofer  C. Goldkamp  J. Ferracone 《Veterinary and comparative oncology》2003,1(1):48-56

The purpose of this study was to characterize canine prostate cancer using immunohistochemical staining specific for acinar and urothelial/ductal tissue and correlate these results with the dogs' castration status/castration time. Seventy dogs with prostate cancer were included, 71% were castrated and 29% were intact. Compared with an age‐matched control population, castrated dogs were at increased risk of prostate cancer, odds ratio 3.9. Immunohistochemical staining was performed on 58 cases. Forty‐six of the 58 stained positive for cytokeratin 7 (CK 7) (ductal/urothelial origin) and one of the 58 stained positive for prostate‐specific antigen. Dogs with CK 7‐positive tumours were younger when castrated than dogs with CK 7‐negative tumours, 2 versus 7 years (P = 0.03); dogs castrated at ≤2 years of age were more likely to be CK 7‐positive (P = 0.009). These results show that most canine prostatic carcinomas are of ductal/urothelial, androgen‐independent origin. This is consistent with the epidemiological findings, showing increased risk in castrated dogs. Canine prostate cancer may, therefore, not be a realistic model for the human disease.  相似文献   

雄激素和氢化可的松对山羊附睾头上皮细胞增殖的影响     

栾兆进  范晓梅  宋慧子  栗瑞兰  张通  张家新 《中国农业大学学报》2019,24(2):91-98

为研究雄激素和氢化可的松对山羊附睾上皮细胞生长的作用模式,本研究利用酶标仪检测附睾头部上皮细胞增殖情况,实时荧光定量PCR及ELISA检测雄激素受体(AR)的表达,检测睾酮与氢化可的松对山羊附睾上皮细胞体外增殖的作用以及对AR表达的影响。结果表明:100nmol/L睾酮对附睾头上皮细胞增殖的促进效应最高且与对照组差异极显著(P0.01),200nmol/L氢化可的松促进附睾头上皮细胞增殖效应最佳并与对照组差异显著(P0.05),前者的效应明显且可以被AR阻断剂阻断;睾酮和氢化可的松对附睾头上皮细胞增殖表现为协同作用;100nmol/L睾酮与200nmol/L氢化可的松均使附睾头上皮细胞的AR mRNA和蛋白表达量上调,与对照组差异显著(P0.05),且二者共存时附睾头上皮细胞的AR mRNA和蛋白表达量极显著高于对照组(P0.01)。本研究表明睾酮和氢化可的松对附睾头上皮细胞体外增殖均有促进作用,呈明显的浓度依赖性,且二者之间存在协同作用,这为进一步研究附睾上皮细胞增殖及功能的调节机理提供了基础。  相似文献   

雄激素受体及共调节因子在睾酮调节绵羊附睾GPX5表达中的作用分析     

栾兆进  赵勇超  许娇霞  刘永斌  张家新 《中国农业大学学报》2023,28(3):94-108

为探究雄激素受体(Androgen receptor,AR)及共调节因子对绵羊附睾上皮细胞(Epididymal epithelial cells,EECs)谷胱甘肽过氧化物酶5(Glutathione peroxidase 5,GPX5)的调节机制,本研究采用CCK-8检测睾酮对EECs增殖的影响;利用qRT-PCR、免疫荧光法和 Western Blot法分别检测AR、GPX5、甾体激素受体共激活子 1(Steroid receptor coactivator 1,SRC-1)、CREB结合蛋白(cAMP-response element-binding protein,CBP)、p300和核受体共抑制因子2(Nuclear receptor corepressor 2,NCOR2)的mRNA水平和蛋白表达情况,采用双荧光素酶报告基因测定干扰SRC-1或p300后EECs的荧光素酶活性。结果表明:1)与对照组相比,100 nmol/L睾酮组细胞中的GPX5 mRNA和蛋白表达量极显著升高(P<0.01),1 000 nmol/L睾酮组细胞中的GPX5 mRNA和蛋白表达量差异不显著(P>0.05),但分别极显著(P<0.01)和显著(P<0.05)低于100 nmol/L睾酮组;100 nmol/L睾酮组细胞中的AR mRNA和蛋白表达量分别呈极显著(P<0.01)和显著(P<0.05)高于对照组,然而1 000 nmol/L睾酮组细胞中的AR mRNA和蛋白的表达量显著低于对照组(P<0.05);1 000 nmol/L睾酮组细胞中的CBP、p300、SRC-1蛋白表达极显著高于对照组(P<0.01),特别是核内的表达量明显增高。2)与对照组相比,siRNA-AR组细胞中的GPX5 mRNA和蛋白的表达量差异不显著(P>0.05),而SRC-1和p300蛋白表达量极显著升高(P<0.01)。pcDNA3.1-AR组GPX5 mRNA和蛋白表达量较对照组呈极显著(P<0.01)和显著(P<0.05)升高。3)siRNA-SRC-1 和siRNA-p300组细胞中的GPX5 mRNA和蛋白表达量较对照组均显著降低(P<0.05),而AR的蛋白表达量与对照组差异不显著(P>0.05),AR的转录活性显著降低(P<0.05)。综上,睾酮对绵羊EECs GPX5、AR及其共调节因子的表达具有浓度依赖性的调节效应,睾酮通过AR及其共调节因子SRC-1和p300/CBP的协同调节GPX5表达。本研究为探究GPX5在绵羊附睾中的调节机制提供理论依据。  相似文献   

Review and analysis of the potential for glyphosate to interact with the estrogen,androgen and thyroid pathways     

Steven L Levine  Elizabeth G Webb  David A Saltmiras 《Pest management science》2020,76(9):2886-2906

Glyphosate was recently evaluated for its potential to interact with the estrogen, androgen and thyroid (EAT) hormone pathways, including steroidogenesis, under the United States Environmental Protection Agency's (USEPA) Endocrine Disruptor Screening Program (EDSP), then by Germany, the rapporteur Member State who led the European Annex 1 renewal for glyphosate, and then by the European Food Protection Agency (EFSA) also as part of the Annex 1 renewal for glyphosate. Under the EDSP, 11 Tier 1 assays were run following the USEPA's validated 890‐series test guidelines and included five in vitro and six in vivo assays to evaluate the EAT pathways. Steroidogenesis was evaluated as part of the estrogen and androgen pathways. An up‐to‐date critical review has been conducted that considered results from the EDSP Tier 1 battery, guideline regulatory studies and an in‐depth analysis of the literature studies that informed an endocrine assessment. A strength of this evaluation was that it included data across multiple levels of biological organization, and mammalian and nonmammalian test systems. There was strong agreement across the in vitro and in vivo Tier 1 battery, guideline studies and relevant literature studies, demonstrating that glyphosate does not interact with EAT pathways including steroidogenesis. Based on an analysis of the comprehensive toxicology database for glyphosate and the literature, this review has concluded that glyphosate does not have endocrine‐disrupting properties through estrogen, androgen, thyroid and steroidogenic modes of action. © 2020 Society of Chemical Industry  相似文献