Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents     

JIN Min  HUANG He-feng  JIN Fan 《园艺学报》2004,20(8):1422-1426

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.  相似文献   

雏鸡脑雌激素受体的表达及分布     

吴建云  范光丽  王鲜忠  张家骅 《中国兽医学报》2005,25(2):189-191

采用免疫组化SP法研究了雌激素受体在lO日龄雏鸡脑组织内的表达，着重观察了雌激素受体在小脑、中脑、下丘脑及端脑的分布。研究表明，雌激素受体主要存在于细胞核中，少数区域仅存于胞浆或胞膜。雌激素受体在脑内分布广泛。在小脑中部皮质的颗粒层、蒲肯野氏层，中脑的中央白质、外侧丘系腹侧核、视束、后连合等区域，雌激素受体免疫反应产物为高密度；在小脑前部皮质的颗粒层，中脑中央灰质、尾侧线形核，端脑副高纹状体等区域，雌激素受体免疫反应产物为中等密度；小脑前部皮质的蒲肯野氏细胞，下丘脑视上核，端脑原始旧纹状体等区域，雌激素受体免疫反应产物为低密度。结果揭示，在鸡脑早期发育过程中，雌激素起著广泛而重要的作用。  相似文献   

鸡粪中雌激素的检测及微生物降解     

郭立泉  崔鹏程  张春玉  徐淼 《农业环境科学学报》2020,37(2):287-292

为检测鸡饲料和鸡粪中雌激素，优化其微生物降解条件，应用高效液相色谱（HPLC）法检测蛋鸡饲料和鸡粪中的雌激素，利用11种高效降解雌激素的单一菌株和等体积混合得到的菌群开展雌激素的降解实验。结果表明：只有蛋鸡鸡粪中含有雌二醇（E2），含量为230 ng·g^-1。微生物菌群的降解率高于单一菌株，在27℃下，10 d内能够降解76%的E2。研究表明，蛋鸡鸡粪中有雌激素存在，微生物菌群对雌激素的降解率高于单一菌株。  相似文献   

电脉冲刺激肾旁穴对兔丘脑雌激素受体蛋白表达的影响     

陈正礼  罗启慧  范光丽  方静  袁朝富  潘康成  牛建荣  杨国林 《畜牧兽医学报》2006,37(1):71-75

采用电脉冲刺激穴位法及免疫组织化学SP法，研究了电脉冲刺激肾旁穴对丘脑内雌激素受体免疫反应阳性产物的影响。结果发现雌激素受体免疫反应阳性产物在丘脑内广泛分布，可见电针组丘脑室旁核、丘脑腹外侧核、丘脑腹内侧核、丘脑腹主核、丘脑中央中核、丘脑网状核、丘脑室周核等核区有大量阳性产物，且多分布于神经元的胞浆、胞核和突起中、有少部分分布于胞膜上，染色较深，突起明显，细胞形态多样，轮廓清晰；而对照组相应神经核团中阳性细胞数量较少且淡染，突起也不明显，形态单一或轮廓不清。两组阳性产物的染色强度和细胞数目均差异显著。以上结果表明电脉冲刺激肾旁穴可使家兔上述核团内雌激素受体的表达增强。  相似文献   

硒营养对蛋鸡强制换羽的影响研究     

宋志刚  呙于明 《中国畜牧杂志》2006,42(7):29-32

本试验旨在通过在日粮中添加不同剂量的硒(0、0.05、0.1、0.2mg/kg日粮),研究蛋鸡换羽过程中激素的变化,探讨硒影响强制换羽的机理。试验结果表明:微量元素硒能够影响换羽过程中血浆T3、T4、促黄体生成激素、黄体酮、雌二醇含量,但是硒参与强制换羽与硒对甲状腺激素代谢的影响无关,硒可能是参与了促黄体生成激素、黄体酮、雌二醇的代谢影响了强制换羽的效果。  相似文献   

Estrogenic activity in coastal areas around Japan evaluated by measuring male serum vitellogenins in Japanese common goby Acanthogobius flavimanus   总被引：1，自引：0，他引：1  

Nobuyuki  OHKUBO  Kazuhiko  MOCHIDA  Shinji  ADACHI  Akihiko  HARA  Komei  HOTTA  Yukio  NAKAMURA  Takahiro  MATSUBARA 《Fisheries Science》2003,69(6):1135-1145

  相似文献   

Changes in estrogen receptor expression in the chick thymus during late embryonic development     

Masafumi Katayama  Tomokazu Fukuda  Toshimitsu Hatabu  Kiyoaki Narabara  Asaki Abe  Yasuhiro Kondo 《Animal Science Journal》2014,85(3):277-285

In chickens, although estrogen receptors (ER) are reported to be associated with the immunological processes, detailed information about the differences in ER expression in the tissues related to the development of lymphocytes is not fully known, especially during the developmental stage. To learn more about this immunological relationship, we used semi‐quantitative polymerase chain reaction method to detect the ER expression levels in the thymus tissues of chicks during the developmental stage. Furthermore, ER‐expressing cells were detected by immunohistochemistry. The results of this study show that the expression level of ER increased on embryonic day 16 and decreased on day 20. Furthermore, ER expression was significantly higher in male than in female chickens at day 16. The increased expression on day 16 and decreased level on day 20 were also reproduced in the incidence of immunoreactive cells, although there was a 1‐day delay in the elevated incidence of the cells. This study revealed the changes in ER expression and the incidence of ER‐positive cells in the thymus of chickens during the developmental stage.  相似文献   

Role of oocyte‐derived paracrine factors in follicular development     

Chihiro Emori  Koji Sugiura 《Animal Science Journal》2014,85(6):627-633

Mammalian oocytes secrete transforming growth factor β (TGF‐β) superfamily proteins, such as growth differentiation factor 9 (GDF9), bone morphogenetic protein 6 (BMP6) and BMP15, and fibroblast growth factors (FGFs). These oocyte‐derived paracrine factors (ODPFs) play essential roles in regulating the differentiation and function of somatic granulosa cells as well as the development of ovarian follicles. In addition to the importance of individual ODPFs, emerging evidence suggests that the interaction of ODPF signals with other intra‐follicular signals, such as estrogen, is critical for folliculogenesis. In this review, we will discuss the current understanding of the role of ODPFs in follicular development with an emphasis on their interaction with estrogen signaling in regulation of the differentiation and function of granulosa cells.  相似文献   

雌二醇对大鼠下丘脑神经激肽B表达的影响     

崔培  宋爽  王尧尧  王瑞瑞  王云  蒋书东  方富贵  李福宝 《中国农业大学学报》2014,19(4):115-120

为研究雌二醇(Estradiol,E2)对大鼠下丘脑神经激肽B(Neurokinin B,NKB)表达的影响,采用免疫组化PV-9003二步法以及DAB显色技术,对外源性E2处理组(E2组)、花生油处理组(C组)、卵巢摘除+E2组(OVX+E2组)和卵巢摘除组(OVX组)大鼠下丘脑的NKB进行检测。结果表明:大鼠阴门开启时间E2组(29.00±0.707)与OVX+E2组(30.20±0.084)早于对照组(43.60±2.074)(P0.01)。NKB主要分布于所有大鼠下丘脑的弓状核、腹内侧核和室旁核,且OVX组和C组室周核上也有少量表达(P0.05),但各组间NKB的平均光密度值有差异,OVX组大鼠下丘脑弓状核(0.33±0.49)、腹内侧核(0.31±0.54)和室旁核(0.30±0.55)均显著高于OVX+E2、C和E2组(P0.05)。雌激素抑制大鼠下丘脑NKB的表达,NKB可能通过雌激素调节生殖作用。  相似文献   

Endocrine and gonadal changes during the annual reproductive cycle of the freshwater teleost,Stizostedion vitreum     

Jeffrey A. Malison  Lynne S. Procarione  Terence P. Barry  Anne R. Kapuscinski  Terrence B. Kayes 《Fish physiology and biochemistry》1994,13(6):473-484

The annual reproductive cycle of walleye (Stizostedion vitreum) was characterized by documenting changes in gonadal development and serum levels of estradiol-17β (E₂), testosterone (T), 17α,20β-dihydroxy-4-pregnen-3-one (17,20-P), and 11-ketotestosterone (11-KT) in wild fish captured from upper midwestern lakes and rivers throughout the year. Fish from the populations used in this study spawn annually in early- to mid-April. Walleye showed group synchronous ovarian development with exogenous vitellogenesis beginning in autumn. Oocyte diameters increased rapidly from ∼ 200 μm in October to ∼ 1,000 μm in November, and reached a maximum of 1,500 μm just prior to spawning. Changes in gonadosomatic indices (GSIs) paralleled changes in oocyte diameters. Serum E₂ levels in females increased rapidly from low values in October (< 0.1 ng ml⁻¹) to peak levels of 3.7 ng ml⁻¹ in November, coinciding with the period of the most rapid ovarian growth. Subsequently, E₂ levels decreased from December through spawning. Serum T levels exhibited a bimodal pattern, increasing to 1.6 ng ml⁻¹ in November, and peaking again at 3.3 ng ml⁻¹ just prior to spawning. We detected 11-KT in the serum of some females at concentrations up to 5.6 ng ml⁻¹, but no seasonal pattern was apparent. In this study (unlike our results in a related study) 17,20-P was not detected. In males, differentiation of spermatogonia began in late August, and by January the testes were filled (> 95% of germ cells) with spermatozoa. Mature spermatozoa could be expressed from males from January through April. GSIs ranged from 0.2% (post-spawn) to 3.2% (pre-spawn). Serum T levels rose from undetectable levels in post-spawn males to 1.6 ng ml⁻¹ by November, remained elevated throughout the winter, and peaked at 2.8 ng ml⁻¹ I prior to spawning. Levels of 11-KT in males remained low (< 10 ng ml⁻¹, from post-spawning through January, then increased significantly by March and peaked just prior to spawning at 39.7 ng ml⁻¹. Our results indicate that vitellogenesis and spermatogenesis are complete or nearly so, in walleye by early winter, and suggest that it may be possible to induce spawning in this species several months prior to the normal spawning season by subjecting fish to relatively simple environmental and hormonal treatments.  相似文献