不同类型花椰菜硫代葡萄糖苷组分与含量分析     

丁云花宋曙辉  赵学志王文琪　何洪巨 《中国蔬菜》2015,1(12):38-43

采用HPLC法测定了3种类型15个花椰菜品种的硫代葡萄糖苷(Glucosinolates,简称硫苷)组分及含量。结果表明:供试花椰菜品种均含有9种硫苷,包括5种脂肪族硫苷:3-甲基硫氧烯丙基硫苷(Glucoiberin,IBE)、2-羟基-3-丁烯基硫苷(Progoitrin,PRO)、2-丙烯基硫苷(Sinigrin,SIN)、4-甲基硫氧丁基硫苷(Glucoraphanin,RAA)和3-丁烯基硫苷(Gluconapin,NAP);4种吲哚族硫苷:4-羟基吲哚基-3-甲基硫苷(4-Hydroxyglucobrassicin,4OH)、3-甲基吲哚基硫苷(Glucobrassicin,GBC)、4-甲氧基吲哚基-3-甲基硫苷(4-Methoxyglucobrassicin,4ME)和1-甲氧基吲哚基-3-甲基硫苷(Neoglucobrassicin,NEO)。各品种的吲哚族硫苷总含量均高于脂肪族硫苷总含量,其中NEO和GBC是花椰菜的主要硫苷组分,分别占总硫苷含量的42.61%和35.02%。不同花椰菜品种的硫苷总含量差异较大,变异范围在0.3323~4.8728μmol·g-1(FW)之间;紫花菜的总硫苷含量最高,松花菜其次,紧花菜最低。  相似文献   

Bacillus thuringiensis CHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants     

Rida Fatima  Tahir Mahmood  Anam Moosa  Muhammad Naveed Aslam  Muhammad Taimoor Shakeel  Ambreen Maqsood  Muhammad Umar Shafiq  Tanvir Ahmad  Mahmoud Moustafa  Mohammad Al-Shehri 《Pest management science》2023,79(1):336-348

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Floral induction (FI) in longan (Dimocarpus longan, Lour.) trees. III: Effect of shading the trees on potassium chlorate induced FI and resulting hormonal changes in leaves and shoots     

K. Sringarm  P. Potchanasin  D. Naphrom  K.F. Bangerth 《Scientia Horticulturae》2009

Previous experiments demonstrated that treatment of longan trees with potassium chlorate (KClO₃) induces “off season floral induction” (FI) even in the absence of the naturally required cool temperature [Manochai, P., Sruamsiri, P., Wiriya-alongkorn, W., Naphrom, D., Hegele, M., Bangerth, F., 2005. Year around off season flower induction in longan (Dimocarpus longan, Lour.) trees by KClO₃ applications: potentials and problems. Sci. Hortic. 104, 379–390]. Potassium chlorate, however, cannot replace the presence of functional mature leaves and sufficient light intensity. Here we examined in more detail the effect of shade (about 10% of natural sunlight) on KClO₃ affected hormone concentrations/transport of leaves and shoot apical buds (SAB) and their interactions with FI.  相似文献   

Two New Cytotoxic Indole Alkaloids from a Deep-Sea Sediment Derived Metagenomic Clone     

Xia Yan  Xi-Xiang Tang  Lin Chen  Zhi-Wei Yi  Mei-Juan Fang  Zhen Wu  Ying-Kun Qiu 《Marine drugs》2014,12(4):2156-2163

Two new indole alkaloids, metagenetriindole A (1) and metagenebiindole A (2), were identified from deep-sea sediment metagenomic clone derived Escherichia coli fermentation broth. The structures of new compounds were elucidated by spectroscopic methods. The two new indole alkaloids demonstrated moderately cytotoxic activity against CNE2, Bel7402 and HT1080 cancer cell lines in vitro.  相似文献   

Alkaloid Accumulation in Catharanthus roseus Increases with Addition of Seawater Salts to the Nutrient Solution   总被引：1，自引：0，他引：1  

WANG Jing-Yan  LIU Zhao-Pu College of Resources  Environmental Sciences  Nanjing Agricultural University  Nanjing 《土壤圈》2010,(6)

A sand culture experiment was conducted to determine the effects of different seawater (5% and 10%) treatments on plant growth,inorganic ions,indole alkaloid concentrations and yields of Catharanthus roseus,in an effort to increase the alkaloid yield by artificial cultivation.The total fresh and dry weights and tissue K + concentrations decreased,but Na + concentrations increased in the plant roots,stems and leaves of C.roseus under seawater stress as compared to the control.The concentrations and yields of...  相似文献   

两品种芜菁中芥子油苷含量的比较分析     

李淑敏  孟令波  高相宇  潘明阳 《北方园艺》2011,(16):1-3

采用HPLC方法测定“红圆”芜菁(HY)和“白玉”芜菁(BY)肉质根和叶片中芥子油苷(GS)成分和含量.结果表明:不同品种芜菁以及同一品种不同部位中芥子油苷(简称GS)含量差别较大.在“红圆”芜菁肉质根中检测出11种GS,在其叶片中检测出9种GS;“红圆”根中主要为苯乙基GS和2-羟基-3-丁烯基GS,分别占总硫苷的37.2％和20.9％,根中总GS含量是叶中的2.1倍.在“白玉”芜菁肉质根中检测出9种GS,主要GS种类为3-丁烯基GS和苯乙基GS,分别占总GS的65.6％和23.4％；在“白玉”芜菁叶片中含8种GS,不合有苯乙基GS,“白玉”叶中GS总量是根中含量的1.2倍.  相似文献   

A rapid chromatographic method for determining the glucosinolate content in crambe seed     

O. Leoni    S. Cinti    N. Aliano  E. D. Tittonel 《Plant Breeding》2003,122(6):517-520

Crambe abyssinica is becoming a reliable source of industrial oil with good technical characteristics because of its high erucic acid content. The possibility of using the industrial defatted meal of crambe as a feed could further promote the interest in this crop, but is prevented by the high glucosinolate content (70‐150 μmol/g). Therefore the possibility of reducing this parameter by selection was evaluated and a search for a new quick analytical procedure begun. When hydrolysed by myrosinase (thioglucoside glucohydrolase EC 3.2.3.1.), at pH 6.5 or higher, epiprogoitrin, the main glucosinolate in crambe seed (>90% of total glucosinolates), gives (5R)‐5‐vinyl‐1,3‐oxazolidine‐2‐thione quantitatively. The latter can be easily estimated by high‐performance liquid chromatography. This is the background of the proposed analytical procedure that permits rapid analysis of about 100 samples per day using about 25 mg of crambe meal (five seeds) for assay. The method can also be used to select for glucosinolate content in rapeseed and, probably with some adjustment, all crops that have a 2‐hydroxy‐glucosinolate as the main glucosinolate.  相似文献   

Glucosinolate content in interspecific crosses of Brassica carinata with B. juncea and B. napus     

A. Getinet    G. Rakow    J. P. Raney  R. K. Downey 《Plant Breeding》1997,116(1):39-46

Brassica carinata A. Braun is a highly productive oilseed crop in the Ethiopian highlands, but the seed has a high 2-propenyl glucosinolate content, which is undesirable. The objective of this study was to introgress, through interspecific crosses, genes for low 2-propenyl glucosinolate content from the B genome of B. juncea and C genome of B. napus into the B. carinata B and C genomes and thus develop low glucosinolate B. carinata. The cross [(B. carinata×B. juncea) ×B. carinata] yielded plants that contained only ～ 20 μmoles of 2-propenyl glucosinolate, which was an 85% reduction compared with levels in B. carinata seed. Plants of the [(B. carinata×B. napus) ×B. carinata] cross had normal high concentrations of 2-propenyl glucosinolate. Backcross plants of both interspecific crosses also contained 3-butenyl and 2-hydroxy-3-butenyl glucosinolates. The results of these crosses suggested that genes for glucosinolate synthesis were located on B genome chromosomes of B. carinata because B. napus C genome introgressions did not result in reductions of total glucosinolate contents. The total alkenyl glucosinolate content of one F₃ family of the B. juncea backcross was similar to that of the B. juncea parent. It was concluded that through further selection in this family, B. carinata plants could be identified that would be basically free of 2-propenyl glucosinolate, and have a low total alkenyl glucosinolate content.  相似文献   

Quality assessment of rapeseed accessions by means of near-infrared spectroscopy on combine harvesters     

J. M. Montes    C. Paul    A. E. Melchinger 《Plant Breeding》2007,126(3):329-330

Optimization of data collection processes in plant breeding programmes is of the highest importance for plant breeders. In rapeseed-breeding, data collection and selection must be carried out in a very restricted period of time because of the short time span between harvesting and sowing. Near-infrared spectroscopy on combine harvesters (NOCH) improves the data collection processes in breeding programmes of grain crops such as maize, and it may also improve the data collection processes in breeding programmes of rapeseed. The objective of this study was to assess the potential of NOCH for the determination of dry matter (DM), crude protein (CP), oil and glucosinolate (GSL) contents in rapeseed. A plot combine harvester equipped with a near-infrared diode-array spectrometer was used. Reference values for DM content were determined by the oven method. Reference values for the quality traits were established by laboratory near-infrared spectroscopy. NOCH showed a high potential for the determination of DM, CP, oil, and GSL contents. Use of NOCH in breeding may increase the efficiency of data collection processes and might dramatically accelerate the development of rapeseed cultivars.  相似文献   

Glucosinolates in the new oilseed crop meadowfoam: natural variation in Section Inflexae of Limnanthes,a new glucosinolate in L. floccosa,and QTL analysis in L. alba     

Pablo Velasco  Mary B. Slabaugh  Ralph Reed  Jennifer Kling  Venkata K. Kishore  Jan F. Stevens  Steven J. Knapp 《Plant Breeding》2011,130(3):352-359

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