硝酸银在水稻农杆菌转化中的作用     

王亚琴  梁承邺 《湖南农业大学学报(自然科学版)》2004,31(2)

以籼稻、粳稻两种类型的成熟种子为外植体研究了不同浓度的AgNO3在水稻农杆菌转化中的作用。结果表明，在农杆菌转化前添加AgNO3可以提高愈伤组织诱导率，最适浓度为8～10mg／L，但对愈伤组织的分化没有明显促进作用；转化后添加低浓度的AgNO3可以减轻愈伤组织的褐化，提高抗性愈伤率和绿苗分化率，最适浓度为2-8mg／L。同时发现，无论是转化前还是转化后，添加高浓度的AgNO3对水稻愈伤组织的分化均具有毒害作用。  相似文献   

家兔黏膜免疫器官肥大细胞在巴氏杆菌感染中的特征——组织化学与电镜观察   总被引：3，自引：0，他引：3  

呼格吉乐图  佘锐萍  刘玉峰  马卫民  胡艳欣 《中国预防兽医学报》2006,28(4):419-422

对5例巴氏杆菌感染家兔的空肠与圆小囊肥大细胞（mast cell，MC）进行了组织化学与电镜的观察，发现在细菌感染导致的病变组织边缘、病变组织内血管附近结缔组织中及变性细胞周围组织的上皮下与黏膜下层都出现了大量甲苯胺蓝（Toluidine Blue，TB）阳性肥大细胞。电镜下，MC有的直接与变性细胞密切接触，有的则和血管内皮紧密相触，在此部位的血管内可见有淋巴细胞贴附填充。MC胞浆内充盈大量的特征性颗粒，有时这些颗粒向细胞表面突出形成边缘空隙，即形成所谓的脱颗粒管道，将颗粒内容物逐渐排除细胞外，遗留空腔。  相似文献   

扫描及负染电镜在兔脑炎原虫病诊断中的应用     

张柳平  段艳  潘耀谦  夏志平  邹啸环 《动物医学进展》2003,24(6):96-97

12只临床表现头颈歪斜、昏睡、震颤、局部或全身轻瘫的可疑为兔脑炎原虫病的獭兔 ,对其肾脏皮髓交界和尿沉渣分别进行了扫描电镜和负染电镜观察 ,看到了大小为 1～ 1 .5× 1 .4～ 2 .5μm,形态呈卵圆形和杆状的兔脑炎原虫。阳性检出率为 1 0 0 %。说明扫描及负染电镜术是诊断兔脑炎原虫病的一种较为可靠的技术。  相似文献   

苹果退绿叶斑病毒组织印迹的免疫法检测与定位     

王乔春 《果树学报》1996,(Z1)

用组织培养技术将富士苹果茎尖外植体建立在ＭＳ培养基中,形成试管苗。取４—６周龄的茎与愈伤组织,徒手切取约１ｍｍ厚的横切面,印迹在硝化纤维膜上。印迹组织经封闭后,与ＣＬＳＶ碱性磷酸酶标抗体反应。对反应结果进行显色。感染ＣＬＳＶ的印迹组织呈紫色反应,正常组织无显色反应。结果表明,该方法十分容易检测印迹组织中的ＣＬＳＶ。带毒愈伤组织较茎的显色反应敏感,显色反应时间仅为茎的一半（１５ｍｉｎ）。ＣＬＳＶ在愈伤组织中有两种分布情况：一是所有组织均有显色反应;二是呈不均匀分布。ＣＬＳＶ在茎组织中有三种分布情况：第一,除髓部外,表皮、皮层及维管系统中均有分布;第二,不均匀分布,ＣＬＳＶ集中分布于表度组织中,在皮层及韧皮部仅有少量分布;第三,“嵌合”分布,即同一种组织中部分组织带毒,部分为正常组织。  相似文献   

Importance of Cell Wall Degrading Enzymes Produced by Fusarium graminearum during Infection of Wheat Heads     

Wanyoike Mary Wanjiru  Kang Zhensheng  Heinrich Buchenauer 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(8):803-810

Cytological studies were carried out to elucidate the importance of cell wall degrading enzymes (CWDE) during infection of wheat spikes by Fusarium graminearum. Scanning electron micrographs revealed that at 6–24 hours after inoculation (hai) of single spikelets with macroconidia of F. graminearum, the fungus germinated by forming several germ tubes and developed a dense hyphal network in the cavity of the spikelet. At 24–36hai, the fungus formed infection hyphae which invaded the ovary and inner surface of the lemma and palea. Transmission electron microscopical studies revealed that the fungus extended inter- and intracellularly in the ovary, lemma and rachis and caused considerable damage and alterations to the host cell walls. In different tissues of healthy and F. graminearum-infected wheat spikes the cell wall components cellulose, xylan and pectin were localized by means of enzyme-gold and immuno-gold labelling techniques. Localization of cellulose, xylan and pectin showed that host cell walls which were in direct contact with the pathogen surface had reduced gold labelling compared to considerable higher labelling densities of walls distant from the pathogen–host interface or in non-colonized tissues. The reduced gold labelling densities in the infected host cell walls indicate that these polysaccharide degrading enzymes might be important pathogenicity factors of F. graminearum during infection of wheat spikes. The results revealed that, infection and colonization of wheat spikes by F. graminearum and reactions of infected host tissue were similar to those reported for F. culmorum.  相似文献   

‘新红星’苹果果实蔗糖合酶的活性及亚细胞定位   总被引：4，自引：2，他引：4  

李培环  董晓颖  王永章  刘成连  原永兵  李鹏 《园艺学报》2002,29(4):375-377

 在‘新红星’苹果果实发育过程中, 伴随果糖、葡萄糖和蔗糖的积累, 蔗糖合酶的分解活性逐渐下降, 而合成活性逐渐升高。苹果果实的蔗糖合酶由分子量为90 kD 的亚基组成, 其免疫信号强度随发育进程而增加。蔗糖合酶主要定位于果肉细胞的细胞质中, 发育中后期该酶的分布密度有一定增加。综合结果认为, 蔗糖合酶调控发育早期蔗糖的降解和发育后期蔗糖的积累。  相似文献   

Detection of Colletotrichum coccodes and Helminthosporium solani in soils by bioassay   总被引：1，自引：0，他引：1  

S. F. Carnegie †  J. W. Choiseul  A. M. I. Roberts 《Plant pathology》2003,52(1):13-21

The sensitivity of a bioassay in detecting soil inoculum of Colletotrichum coccodes and Helminthosporium solani was examined using potato minitubers and microplants. Tests were conducted on soils which were collected from fields in which the interval after a previous potato crop differed, and which were also artificially infested with conidia or microsclerotia. For C. coccodes , determining plant infection based on the occurrence of infected roots after 9–12 weeks was a sensitive method for detecting and quantifying the amount of inoculum in soil. Infestations of less than 0·4 microsclerotia per g soil were detected in artificially infested soils. A semiselective medium, developed for isolating C. gloeosporioides from pepper, detected soil infestations by C. coccodes as low as nine conidia or one microsclerotium per g soil in artificially infested soil. For H. solani , infection on minitubers was a sensitive measure, with soil inoculum of fewer than 10 conidia per g soil being detected. Soil infestation could be quantified by assessing the percentage surface area of minitubers covered by sporulating lesions, which was strongly related to the amount of soil infestation. The results of these bioassay tests were compared with published results for real-time quantitative PCR assays on the same soils. The two methods were in good agreement in artificially infested soils, but the bioassay appeared to be more sensitive with naturally infested soils.  相似文献   

Expression and subcellular localization of urocortin in syncytiotrophoblast of human term placenta     

ZHU Ying-yuan  WANG Zi-neng  ZENG Yi-ke  ZHENG Pei-e  XU Jian-ping  GUO Zu-wen  TANG Fu-xing 《园艺学报》2004,20(6):1038-1041

AIM: To obverse the expression and localization of urocortin on ultrathin cryosections of syncytiotrophoblast of human term placenta with immunocytochemistry technique under transmission electron microscope. METHODS: The human term placenta tissue from Cesarean delivery and normal labor were fixed in 4% paraformaldehyde, and then divided into two parts. One part was for regular immunocytochemistry under microscope, and the other part was used to prepare ultrathin cryosections for immunocytochemistry under transmission electron microscope. RESULTS: 1.Uroncortin mainly distributed in cytoplasm of syncytiotrophoblast of human term placenta under microscope. Urocortin also appeared in cytoplasm in some stromal cells. 2. Under transmission electron microscope, the anti-urocortin gold particles were observed in cytoplasm of syncytioptrophoblast ultrathin cryosections and sited on rough-surfaced endoplasmic reticulum. The anti-urocortin gold particles also appeared on nucleus and nuclear membrane of syncytiotrophoblast. CONCLUSION: Syncytiotrophoblast of human term placenta synthesized and secreted urocortin. The internalization of urocortin within syncytiotrophoblast nuclear indicates that urocortin may act as intracrine.  相似文献   

VEGF在绵羊生殖管道中的表达规律     

葛晨霞  王龙涛  贾博寅  刘莉  王瑞雪  姜怀志 《中国兽医学报》2011,31(12)

以不同发情周期雌性绵羊子宫、输卵管为研究对象,采用免疫组织化学技术,针对血管内皮生长因子（VEGF）在绵羊子宫、输卵管的表达、定位和变化规律进行了检测,同时应用相关图像分析软件对抗原染色强度进行了定量分析。结果表明：输卵管在发情0～15d,VEGF表达量在第9天达到峰值后经历波动逐渐下降过程,输卵管内膜上皮细胞是VEGF抗原的主要靶细胞;而子宫角在发情0～15d,VEGF表达量在第5天达到峰值后经历波动逐渐下降过程,子宫内膜固有层及腺体周围细胞为VEGF抗原的主要靶细胞。该研究结果为绵羊生产中进一步提高受胎率和妊娠率及频密产羔等技术的应用提供了科学依据。  相似文献   

苦瓜转录因子McEIL2的克隆及其表达分析     

高山  许端祥  陈中钐  谢鑫鑫  钟开勤  林碧英  温庆放 《分子植物育种》2015,(6)

本研究从已构建的苦瓜果实均一化文库筛选得到一个EIN3-like同源EST序列,结合RACE技术,克隆得到EIN3基因c DNA全长序列,命名为Mc EIL2(Gen Bank登录号:KF595122)。该基因全长2 122 bp,开放阅读框1 890 bp,编码629个氨基酸,预测该蛋白的分子量为71.58 k D,与黄瓜Cus EIN3、甜瓜Cm EIL1、苜蓿Mt EIL1、绿豆Vr EIL1和番茄Le EIL1的蛋白同源性分别为89.61%、78.37%、71.23%、69.09%和65.66%。Mc EIL2基因的编码蛋白亚细胞定位于细胞核,荧光定量分析表明Mc EIL2基因表达量在幼果期先强后弱,绿熟期最低,破色期表达量大幅增加至最高随后下降,推测该基因参与苦瓜果实成熟软化调控过程。本研究初步明确了Mc EIL2基因在苦瓜成熟过程表达模式,可为今后进一步揭示苦瓜果实成熟软化的分子机制奠定基础。  相似文献