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Abstract— The microhabitat selection of stone loach ( Barbatula barbatula ) in relation to instream cover and predation risk was investigated in artificial streams. In one experiment, stone loach were presented different combinations of shelter structures, two at a time, that provided visual isolation, flow refuge, both, or none. In all cases, visual isolation shelters were selected by the fish, with little use of clear shelters and no apparent selection of flow refuges. In a second experiment, diel activity patterns and habitat selection of stone loach were measured when visual isolation structures were placed in the riffle only, pool only, both riffle and pool or in no habitat. Stone loach were marked with passive integrated transponder tags and a sensor plate was placed between the riffle and pool habitats to measure diel activity patterns. Habitat use was measured by releasing drop gates between the two habitats at the end of each trial. More stone loach used the pool than the riffle in all treatments, but use of the riffle increased when cover was present only there. Furthermore, stone loach were most active between 2100 and 0300. To determine the effect of predation threat on habitat use by stone loach, one adult brown trout ( Salmo trutta ) was added to each pool. The presence of trout caused stone loach to move into the riffle, especially the smaller loach. Overall, the study showed that stone loach used cover in a visual isolation context, preferring deeper water when there was no predation risk, but made more use of shallower water in the presence of brown trout. 相似文献
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Jong-Chang Tsai 《Fish physiology and biochemistry》1996,15(4):307-316
The pattern of cytokeratin proteins in the epidermal cells of loach was studied by immunotechniques and partial separation of the epidermal cells. Two monoclonal antibodies, namely 8F7 and 1C45, against the cytokeratin proteins of the loach epidermis were prepared. these two monoclonal antibodies exhibit distinctive results in immunohistochemical staining. The 8F7 monoclonal antibody stains mainly with the epithelial cells, while the 1C45 monoclonal antibody stains specifically with the club cells. The pattern of cytokeratin proteins in the club cells and the epithelial cells of various epidermal layers was further determined by partial separation of these cells. Immunoblotting analysis of the cell fractions confirms the cytokeratin proteins to be differentially expressed in the club cells and the epithelial cells. However, the cytokeratin proteins expressed in the epithelial cells of the basal, middle and outer layers are same. The results indicate that differentiation of the epithelial cells seems limited during their translocation from basal to upper layers, but in those cells that do differentiate into club cells, the cytokeratin pattern changes. 相似文献
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以鲜活泥鳅为试材，经带骨、去骨、不同预煮、添加不同粘结剂等处理后，研制了一种新型泥鳅糜干制品──调味泥鳅脯。结果表明，带骨原料经姜煮后，以豆粉作为粘结剂，其产品中钙、蛋白质含量最高，感官质量较好。 相似文献
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本研究从来自黑龙江及广州地区的泥鳅中分离到10条颚口线虫幼虫,采用光学显微镜观察幼虫的形态特征,并对虫体的ITS2与CO1基因进行扩增测序、系统发育分析以鉴定虫种。结果显示10条颚口线虫幼虫头球均有3环小钩,其形态学特征与日本颚口线虫(Gnathostoma nipponicum)第3期幼虫相符。序列分析结果显示与GenBank中登录的日本颚口线虫ITS2和CO1基因的同源性分别为100%和99%。邻位连接法构建的50%一致树也均与日本颚口线虫处于同一分支。表明从黑龙江及广州地区泥鳅中分离的颚口线虫幼虫为日本颚口线虫幼虫,首次证明了中国存在日本颚口线虫。 相似文献
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稻鳖鱼虾鳅生态种养关键在于选择优质水稻品种及优质水生动物品种.从水稻种植、稻田管理、水生动物养殖、病虫害防治等方面介绍了稻鳖鱼虾鳅生态种养技术. 相似文献
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采用中性蛋白酶水解泥鳅蛋白制备抗氧化肽,以DPPH自由基清除能力为指标,用木瓜白酶对泥鳅蛋白抗氧化肽进行Plastein反应修饰研究。通过单因素试验研究了外源氨基酸种类、pH值、时间、E/S、温度和外源氨基酸的添加量对泥鳅蛋白抗氧化肽Plastein反应修饰的影响,在此基础上采用响应面法对泥鳅蛋白抗氧化肽Plastein反应修饰工艺进行了优化。结果表明,泥鳅蛋白抗氧化肽Plastein反应修饰的最佳条件为:底物浓度40%、组氨酸添加量为0.5 mmol·g-1、E/S为1 782.49 U·g-1 、pH值9.0、温度35 ℃、时间3 h,此条件下制备的Plastein反应修饰产物的抗氧化活性是修饰前的1.99倍,其对DPPH自由基的清除率为(77.98±0.08)%。 相似文献
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