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排序方式: 共有98条查询结果,搜索用时 31 毫秒
1.
Attachment and adhesion of conidia of a wheat-isolate of Stagonospora nodorum to leaf and artificial surfaces was studied. Attachment of conidia was a non-viable process, separate from adhesion, that occurred rapidly and irreversibly. Attachment involved conidial-surface carbohydrates and was partially influenced by surface hydrophobicity. The subsequent adhesion, via the secretion of extracellular matrix from conidia, was a viable process that induced the complete cover of conidia in response to wheat leaf surface components containing epi-cuticular wax and to a lesser extent to barley but inducing only partial covering on glass. Results suggest that specific surface components from the compatible host promote rapid attachment and adhesion of S. nodorum conidia.  相似文献   
2.
The spores (conidia) of the bean anthracnose fungal pathogen, Colletotrichum lindemuthianum, adhere to the aerial parts of plants to initiate the infection process. In previous studies we have shown that the Colletotrichum spores are surrounded by a fibrillar spore coat, comprising several major glycoproteins. Previous evidence showed that a monoclonal antibody (UB20) that recognised these glycoproteins was able to inhibit adhesion of spores to a hydrophobic surface. In this paper we have further studied the role of the spore coat in adhesion, germination and fungal development by studying the effects of UB20 and protease treatment of spores. The latter treatment has previously been shown to remove the spore coat. Spores germinate on glass, polystyrene and water agar, however, appressoria only develop on glass or polystyrene, showing a requirement for a hard surface. Removal of the spore coat with protease inhibits adhesion at 30 min, before the secretion of ECM glycoproteins. Protease treatment also inhibits the development of appressoria and reduces pathogenicity on leaves. Incubation of spores with the MAb UB20 inhibits adhesion at 30 min, but does not affect appressorium formation or pathogenicity. The results suggest that an intact spore coat has two functions; it is required for adhesion to a hydrophobic surface and for the detection of a hard surface necessary for appressorium formation. We suggest that contact with a hard surface, rather than adhesion, is the key event leading to appressorium formation.  相似文献   
3.
Fifty-nine monoclonal antibodies (mAb) were assigned to the adhesion section of the Second International Swine CD Workshop. They were analysed for their reactivity to selected lymphoid cell populations, as well as to non-lymphoid cell lines. Cell lysate ELISAS and Western Blot analyses were also carried out. As a result, thirteen separate cluster groups emerged (p>0.95). Workshop assignments for adhesion molecules were made: wCD29/49 for mAbs UCP1D2 (#133) and FW4-101 (#165), and PNK-I (#194) and MUC76A (#025) could be assigned to wCD18. For one cluster (FQ1D7, #161 and 2F4, #069) the cellular distribution and MW were characteristic for MHC Class II, and another cluster comprising several antibodies which appeared to recognise MHC Class I. Other clusters could not be assigned to cell surface structures known to be linked to cellular adhesion, however, two further antibodies, 335-2 (#112) and FG1F6 (#156), could be added to SWC1, and the new SWC8 was defined by MIL3 (#077) and MUC20A (#029), binding a ligand of 29–32 kDa. Clustering for these two antibodies was confirmed by blocking studies. The cellular distribution is known for MIL3, recognising an epitope present on granulocytes, B cells, and a subset of T cells expressing CD8 at high intensity.  相似文献   
4.
胶合板胶合效果评价方法探讨   总被引:4,自引:0,他引:4  
现行胶合板标准采用传统的胶合强度指标(含木破率)评判胶合板的胶合性能具有一定的局限性。本文通过对胶合板胶合强度的分析和胶合界面的观察,包括显微观察,提出以胶合有效性、木材破坏率和胶合层剪切弹性模量或其综合评分为衡量指标,是评判胶合板胶合效果较为客观的方法。  相似文献   
5.
6.
减粘脱附柔性内衬的设计与应用   总被引:5,自引:1,他引:5  
针对挖掘、装卸及运输机械的与物料接触部件,在以粘性物料为工作对象时明显存在物料粘附积留问题,依据典型脱土动物功能防粘机制所提供的信息,设计了具有明显减粘脱附效果的柔性内衬,并进行了柔性内衬用于滚轮堆取料机轮斗、挖掘机挖斗及装载机铲斗的实际试验。文中介绍了柔性内衬结构特点、设计方法及实际试验结果。  相似文献   
7.
Choi YJ  Yoon JH  Cha SW  Lee SG 《Fitoterapia》2011,82(6):911-919
Ginsenoside Rh1 has been reported to possess antiallergic and anti-inflammatory activities, but its effects on monocytes remain to be determined. Herein, we investigated the effects of Rh1 on the expression of MCP-1 and CCR2, activation of MAPK signaling, and chemotaxis of monocytes. Treatment of Rh1 decreased the levels of MCP-1 and CCR2 and the expression of VLA5 and activated β1 integrin on the cell surface, and attenuated the phosphorylation of MAPKs. Based on these results, the inhibitory effects of Rh1 on monocyte function should be regarded as a promising new anti-inflammatory response with a potential therapeutic role against inflammation-dependent diseases.  相似文献   
8.
用以化学吸附为主的土壤粘附模型,对法向粘附、切向粘附和无水情况下的粘附规律以及界面压力、加压时间、温度变化等因素对粘附力的影响进行了分析,该粘附机理不仅能够很好地解释粘附规律,而且还能对已有粘附理论尚解释不清的粘附现象,如土壤机械组成、矿物质组成与粘附力的关系,粘附力与吸附阳离子的关系,pH值对粘附力的影响等作出解释。在此基础上,作者根据这一模型提出了改变触土部件表面材料组成、触土部件非光滑表面仿生改形和电渗减粘脱土的有效方法  相似文献   
9.
界面粘附中非光滑表面基本特性的研究   总被引:4,自引:3,他引:4  
某些非光滑表面比光滑表面更能减小运动阻力,这是有悖于传统认识的最新发现。本文从界面粘附角度提出了光滑与非光滑表面的基本概念,分析了非光滑表面的种类及其特性,阐述了非光滑表面仿生研究及其应用的现代发展,并展望了非光滑表面仿生降阻的前景。  相似文献   
10.
典型生物柔性非光滑体表的防粘研究   总被引:14,自引:5,他引:14  
在分析蚯蚓、马陆、穿山甲、田鼠等典型土壤动物柔性体表形态及其作用的基础上,分析了柔性非光滑特征及其防粘特性,并进行了原理性的试验研究,为开发柔性仿生技术奠定了基础  相似文献   
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