Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model     

LI Zhong  ZHANG Cheng  CHEN Guo-jun  LIU Xiao-rong 《园艺学报》2004,20(3):311-316

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.  相似文献   

苹果梨花芽孕育期生长锥细胞超微结构的观察   总被引：1，自引：0，他引：1  

郭金丽  张玉兰  郝喜龙 《果树学报》2001,18(4):213-216

利用透射电镜对苹果梨花芽孕育期芽内生长锥细胞进行了观察，结果显示，在花芽孕育形成的过程中，生长锥细胞的超微结构发生了一系列变化：蛋白体和脂类小滴逐渐增加；核外膜向外延伸到胞质中与内质网相连，其上分布的核糖体增多；线粒体和内质网数目丰富且分布均匀；核糖体大量游散在胞质中；质体呈浅裂，中央缢缩；微管分布于靠近质膜处等。以上超微结构的变化表现出细胞旺盛分裂和分化的特点，反映了花芽孕育过程中的细胞学特征。  相似文献   

沼液浸种增强早稻秧苗耐冷效应─—叶肉细胞超微结构动态观察     

朱斌成  魏向文 《江西农业学报》1995,7(2):83-87

经沼液浸种的早稻秧苗比对照组秧苗，具有较强的耐冷性，通过电镜对叶肉细胞超微结构动态观察证实，在低温（５°／５℃）下，叶绿体结构和细胞核结构，随着冷害天数的延长，伤害在不断加重，叶绿体结构会出现空洞，基质片断裂，直至崩溃。细胞核染色体凝集，核膜破裂，核内容物丧失等现象出现。而经沼液浸种的秧苗伤害程度有较明显的减轻。  相似文献   

冰核细菌对仁用杏胚珠超微结构的影响   总被引：10，自引：0，他引：10  

彭伟秀  杨建民  张芹  孟庆瑞 《园艺学报》2004,31(1):21-24

 应用透射电镜对两个仁用杏品种‘白玉扁’和‘一窝蜂’接种冰核细菌并低温处理后的胚珠超微结构进行了观察, 发现冰核细菌对胚珠的超微结构有一定影响: (1) 使珠心细胞发生严重的质壁分离,细胞质中存在大量泡状结构, 线粒体的内部结构完全被破坏, 呈透明状, 而且有些珠心细胞中形成同心圆状的多膜内含物; (2) 使胚囊中卵细胞的细胞核外膜膨胀, 甚至核膜局部解体, 细胞质中细胞器减少。这些变化致使胚珠发育不正常, 影响受精而导致减产或绝收。接种冰核细菌并低温处理比单纯低温处理对胚珠超微结构的破坏程度重。  相似文献   

苯丙烯酸对黄瓜幼苗光合作用和细胞超微结构的影响   总被引：13，自引：0，他引：13  

吴凤芝  潘凯  马凤鸣  王学东 《园艺学报》2004,31(2):183-188

 通过基质栽培研究不同浓度的苯丙烯酸对黄瓜幼苗光合作用和超微结构的影响。结果表明，苯丙烯酸浓度在80 µmol·L 时显著地降低了黄瓜幼苗的叶面积、叶绿素a含量、光合速率、蒸腾速率、根系活力和气孔张开数(P<0.05)；叶绿体和线粒体等超微结构也受到破坏，而浓度在150 µmol·L 时显著减少了全株干质量和气孔总数，细胞器出现解体现象。  相似文献   

Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice     

ZHANG Xu  LIU Ming-ming  CHENG Ming  XU Jin  WANG Shu-he  WANG Hui  REN Ya-li  XIU Rui-juan  WANG Su-xia 《园艺学报》2015,31(8):1520

AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.  相似文献   

大豆属Clycine亚属植物花粉形态研究   总被引：2，自引：0，他引：2  

庄炳昌  席以珍 《作物学报》1996,22(3):279-282

对大豆属Ｇｌｙｃｉｎｅ亚属中０个种的花粉进行了光学显微镜和扫描电镜的观察。发现多倍体材料的花粉大于二倍体的。并发现Ｇｌｙｃｉｎｅ亚属诸种花粉的形、萌发孔、外壁纹饰均存明显差异。  相似文献   

水分胁迫对冬小麦旗叶细胞质膜及叶肉细胞超微结构的影响   总被引：32，自引：1，他引：32  

许振柱  于振文 《作物学报》1997,23(3):370-375

随着水分胁迫程度的加剧，冬小麦叶的膜质过氧化物丙二醛的含量升高，膜脂 酸不饱和度降低，叶肉细胞内叶绿体、线粒体的形态、结构异常，甚至裂解，质膜内陷，胞液泡化，胞壁层次减少。表明土壤水分胁迫损害小麦旗叶细胞质膜及叶肉细胞的超微结构，加速衰老进程。  相似文献   

蛇腹鳞表面的超微结构及减阻机理     

张占立  杨继昌  丁建宁  胡友耀  解国新 《农业机械学报》2007,38(9):155-158

用原子力显微镜观测了竹叶青蛇、短尾蝮蛇和赤链华游蛇腹鳞表面的超微结构,分析了腹鳞表面的减阻机理。结果表明:3种蛇腹鳞表面的超微结构均为微凸体、微孔和凹坑周期排列的规律结构;蛇的栖息环境不同,超微结构的排间距、微凸体长度以及微孔的直径、数量不同。从陆栖到水栖,微凸体长度逐渐减小,微孔数量逐渐增加。微凸体减少了腹鳞和地面间的实际接触面积,从而减小了滑动时的粘着力;腹鳞表面的疏水性,减少了接触面间水的粘附力。  相似文献   

甜菜花叶病毒病研究之二——感病细胞超微结构观察     

元平  裘维蕃 《植物病理学报》1994,24(3):223-226

 对不同时期感染甜菜花叶病毒(BtMV)的叶片,进行病理变化系列观察。早期侵染(7天前)的细胞质、细胞器均无变化,随着症状发展,风轮状内含体、束状内含体、细胞核卫星体,细胞质泡囊化旁类似病毒的束状结构,20天达到了高峰,风轮状及束状内含体一直持续两个月还存在。田间样品也观察到了典型的风轮状及束状内含体。细胞核卫星体出现很少。侵染后期,细胞质减少,叶绿体比相应健株提前出现淀粉粒,噬锇颗粒等,基粒片层也提前消解,表现细胞提前衰老的特征。此外细胞内还有许多健株没有的泡状结构。一种为旁壁体,可能与细胞壁加厚有关;一种为多重泡体的叠加,可能是液泡的吞噬作用加强与外源物质(BtMV)侵入有关;另外还有一种可能是过氧化物酶体或线粒体在病毒诱导下泡囊化,其作用不清楚。  相似文献