全文获取类型
收费全文 | 86篇 |
免费 | 4篇 |
国内免费 | 14篇 |
专业分类
林业 | 2篇 |
农学 | 1篇 |
1篇 | |
综合类 | 23篇 |
农作物 | 2篇 |
水产渔业 | 25篇 |
畜牧兽医 | 47篇 |
园艺 | 1篇 |
植物保护 | 2篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2017年 | 6篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 13篇 |
2012年 | 9篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 1篇 |
2008年 | 10篇 |
2007年 | 4篇 |
2006年 | 3篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有104条查询结果,搜索用时 15 毫秒
1.
The use of cultured hepatocytes from goats and cattle to investigate xenobiotic oxidative metabolism
C. Montesissa P. Anfossi G. Van't Klooster M. Mengelers 《Veterinary research communications》1996,20(5):449-460
The oxidative metabolism of aldicarb (ALD), a carbamate pesticide, and fenbendazole (FBZ), an anthelmintic, was studied using cultured hepatocytes obtained from 4 goats and a bullock and incubated with ALD (50 mol/L) and FBZ (10 mol/L). The parent compounds and the metabolites were measured by HPLC. Both compounds are metabolized at the sulphur atom via two sequential oxidations, first to the sulphoxide (aldicarb sulphoxide and oxfendazole, respectively) and then to the sulphone. Oxfendazole and fenbendazole sulphone from FBZ, and aldicarb sulphoxide from ALD were found in both species. Aldicarb sulphone was not produced by the hepatocyte preparations from the bullock. The good correlation obtained comparing the in vitro results of FBZ metabolism with published in vivo dat obtained on FBZ kinetics in the same species confirmed the usefulness of in vitro models for predictive analysis of in vivo xenobiotic biotransformations.Abbreviations ALD
aldicarb
- ALDSON
aldicarb sulphone
- ALDSOX
aldicarb sulphoxide
- BSA
bovine serum albumin
- ID
internal diameter
- EGTA
ethylene glycol bis(-aminoethyl ether) N,N,N,N-tetraacetic acid
- FBZ
fenbendazole
- FBZSON
fenbendazole sulphone
- HBSS
Hanks' balanced saline solution
- HPLC
high-pressure liquid chromatography
- LDH
lactate dehydrogenase
- MFO
mixed function oxidase
- NCS
newborn calf serum
- OXF
oxfendazole
- WME
Williams' Medium E 相似文献
2.
为探明虎杖对鱼类损伤肝细胞是否具有修复作用,分离建鲤肝细胞,设6个试验组:I组(空白对照组);II组(CCl4模型组);III组(800 μg/mL虎杖提取物对照组);IV组(造模前200、400、800 μg/mL虎杖提取物处理组);V组(造模后200、400、800 μg/mL虎杖提取物处理组);VI组(造模前、后200、400、800 μg/mL虎杖提取物处理组)。各组细胞经处理后继续培养12 h,收集肝细胞培养液,检测上清培养液中丙氨酸转氨酶(GPT)、天冬氨酸转氨酶(GOT)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)等酶活性,丙二醛(MDA)含量及肝细胞的存活率。结果表明:III组肝细胞培养液中GOT、GPT、LDH、SOD、MDA值及细胞存活率与I组相比无明显变化(P>0.05),说明800 μg/mL虎杖提取物没有细胞毒性,可用于后续试验;与II组相比,IV组中800 μg/mL虎杖提取物处理组的效果优于其他2个浓度组,可以极显著降低培养液中GOT值(P<0.01),显著降低培养液中GPT、LDH值(P<0.05),显著提高肝细胞的存活率(P<0.05),而MDA含量和SOD值差异无统计学意义(P>0.05);与II组相比,V组中400 μg/mL虎杖提取物效果优于其他2个浓度组,可以极显著降低培养液中GOT值(P<0.01),显著降低培养液中LDH值(P<0.05),显著提高肝细胞的存活率(P<0.05),但GPT值、MDA含量、SOD的差异无统计学意义(P>0.05);与II组相比,VI组中800 μg/mL虎杖提取物效果优于其他2个浓度组,能极显著降低培养液中GOT、GPT、LDH在肝细胞中的释放(P<0.01),显著降低培养液中MDA含量(P<0.05),显著提高肝细胞的存活率(P<0.05)。综合以上结果,以VI组中800 μg/mL的虎杖提取物效果最好,能有效抑制CCl4所造成的肝细胞损伤,其机制可能与其抗氧化作用有关。 相似文献
3.
为研究线粒体融合蛋白2(mitofusin 2,MFN2)对高β-羟丁酸(β-hydroxybutyrate,BHBA)活化NF-κB炎性通路的影响,体外培养奶牛肝细胞,添加不同浓度(0.0,1.2,2.4,4.8 mmol/L)的BHBA,并转染过表达MFN2的腺病毒,运用Western blot和qRT-PCR技术检测NF-κB炎性通路关键分子的基因和蛋白表达。结果显示,随着BHBA浓度的增加,IKBα和NF-κB p65的磷酸化水平以及IL-1β、IL-6和TNFα的mRNA表达均显著升高,MFN2的基因和蛋白表达水平则显著降低;过表达MFN2后,显著抑制了高BHBA诱导的IKBα和NF-κB p65磷酸化水平及IL-1β、IL-6、TNFαmRNA表达水平的升高。结果表明,在奶牛肝细胞中过表达MFN2可以显著抑制高BHBA活化的NF-κB炎性通路。 相似文献
4.
5.
Geun Hye Hwang Yu Jin Jeon Ho Jae Han Soo Hyun Park Kyoung Min Baek Woochul Chang Joong Sun Kim Lark Kyun Kim You-Mie Lee Sangkyu Lee Jong-Sup Bae Jun-Goo Jee Min Young Lee 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):17-23
Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H2O2 in a dose-dependent manner. Additionally, H2O2 treatment increased Bax, decreased Bcl-2, and promoted PARP-1 cleavage in a dose-dependent manner. Pretreatment with BHA before exposure to H2O2 significantly attenuated the H2O2-induced decrease of cell viability. H2O2 exposure resulted in an increase of intracellular reactive oxygen species (ROS) generation that was significantly inhibited by pretreatment with BHA or N-acetyl-cysteine (NAC, an ROS scavenger). H2O2-induced decrease of cell viability was also attenuated by pretreatment with BHA and NAC. Furthermore, H2O2-induced increase of Bax, decrease of Bcl-2, and PARP-1 cleavage was also inhibited by BHA. Taken together, results of this investigation demonstrated that BHA protects primary cultured mouse hepatocytes against H2O2-induced apoptosis by inhibiting ROS generation. 相似文献
6.
7.
J. FONSECA-MADRIGAL V. KARALAZOS P.J. CAMPBELL J.G. BELL & D.R. TOCHER 《Aquaculture Nutrition》2005,11(4):241-250
This study aimed to investigate the effects of dietary crude palm oil (CPO) on fatty acid metabolism in liver and intestine of rainbow trout. Triplicate groups of rainbow trout for 10 weeks at 13 °C were fed on diets in which CPO replaced fish oil (FO) in a graded manner (0–100%). At the end of the trial, fatty acid compositions of flesh, liver and pyloric caeca were determined and highly unsaturated fatty acid (HUFA) synthesis and fatty acid oxidation were estimated in isolated hepatocytes and caecal enterocytes using [1‐14C]18:3n‐3 as substrate. Growth performance and feed efficiency were unaffected by dietary CPO. Fatty acid compositions of selected tissues reflected the dietary fatty acid composition with increasing CPO resulting in increased proportions of 18:1n‐9 and 18:2n‐6 and decreased proportions of n‐3HUFA, 20:5n‐3 and 22:6n‐3. Palmitic acid, 16:0, was also increased in flesh and pyloric caeca, but not in liver. The capacity of HUFA synthesis from 18:3n‐3 increased by up to threefold in both hepatocytes and enterocytes in response to graded increases in dietary CPO. In contrast, oxidation of 18:3n‐3 was unaffected by dietary CPO in hepatocytes and reduced by high levels of dietary CPO in enterocytes. The results of this study suggest that CPO can be used at least to partially replace FO in diets for rainbow trout in terms of permitting similar growth and feed conversion, and having no major detrimental effects on lipid and fatty acid metabolism, although flesh fatty acid compositions are significantly affected at an inclusion level above 50%, with n‐3HUFA reduced by up to 40%. 相似文献
8.
Previous studies have reported very low rates of gluconeogenesis from lactate in sea raven (Hemitripterus americanus) hepatocytes compared to other teleosts studied. This study examines whether hepatic cell redox or lactate dehydrogenase
(LDH) characteristics may explain this observation. Sea raven hepatic optimal LDH activities (pyruvate reductase direction)
were more than 40 times less compared with rainbow trout liver values (40 vs 1914 μmol·min−1·g−1 protein). The Km(lactate) was 9.24 and 0.86 mM for sea raven and trout hepatic LDH, but the Km(pyruvate) was similar between
the two species (0.11 and 0.21 mM, respectively). These results suggested that sea raven liver LDH did not favour lactate
use and was more indicative of the mammalian M-isozyme. Gel electrophoresis showed a predominant intermediate isozyme, with
a small amount of the M-type LDH. Phosphoenolpyruvate carboxykinase (PEPCK) was localized to the mitochondrial compartment,
while there was no apparent mitochondrial glutamate-oxaloacetate transaminase (GOT) activity. No in vitro lactate flux to glucose was found in untreated, 10 mM ethanol-treated, or 3 mM NH4Cl-treated sea raven hepatocytes, although CO2 production from lactate was decreased by ethanol and increased by NH4Cl. These results provide evidence that cell redox does not limit gluconeogenesis from lactate, while low activities and the
kinetic characteristics of LDH may partially explain the low lactate gluconeogenesis reported in sea raven hepatocytes.
To whom correspondence should be addressed at University of Ottawa. 相似文献
9.
The static or declining supply of fish oil from industrial fisheries demands the search of alternatives, such as plant (vegetable)
oils, for diets in expanding marine aquaculture. Vegetable oils are rich in C18 polyunsaturated fatty acids but devoid of the n-3 highly unsaturated fatty acids in fish oils. Previous studies, primarily
with salmonids, have shown that including vegetable oils in their diets increased hepatocyte fatty acid desaturation. In the
present study, we have investigated the effects of dietary partial substitution of fish oil (FO) with rapeseed oil (RO), linseed
oil (LO) and olive oil (OO) on the desaturation /elongation and, -oxidation capacities of [1-14C]18:3n-3 in isolated hepatocytes from European sea bass (Dicentrarchus labrax L.), in a simultaneous combined assay. Fish were fed during 34 weeks with diets containing 100% FO, or RO, LO and OO, each
included at 60% with the balance being met by FO, with no detrimental effect upon growth or survival. The highest total desaturation
rates were found in hepatocytes of fish fed FO diet (0.52±0.08 pmol/h/mg protein) and OO diet (0.43±0.09 pmol/h/mg protein),
which represented 3.2% and 2.7% of total [1-14C]18:3n-3 incorporated, respectively. In contrast, lowest desaturation rates were presented by hepatocytes of fish fed LO
and RO diets (0.23±0.06 and 0.14±0.05 pmol/h/mg protein, respectively) represented 1.4% and 0.9% of total [1-14C]18:3n-3 incorporated, respectively. The rates of [1-14C]18:3n-3 β-oxidized were between 11-fold and 35-fold higher than desaturation. However, no significant differences were observed
among β-oxidation activities in hepatocytes of fish fed any of the diets. The present study demonstrated that the European
sea bass, as a carnivorous marine fish, presented a ‘marine’ fish pattern in the metabolism of 18:3n-3 to 20:5n-3 and 22:6n-3.
This species appeared to have all the enzymic activities necessary to produce 22:6n-3 but presented only extremely low rates
of fatty acid bioconversion. Furthermore, nutritional regulation of hepatocyte fatty acid desaturation was minimal, and dietary
vegetable oils did not increase desaturase activities, and in RO and LO treatments the activity was significantly lower.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Distribution and function of hepatic - and -adrenoceptors were examined in rainbow trout (Oncorhynchus mykiss) injected with slow release hydrogenated coconut oil implants alone (sham) or containing cortisol. - and -Adrenoceptors were assayed on purified hepatic membranes 10–14 days post-implantation using 3H-prazosin () and 3H-CGP (). At 10–14 days, plasma cortisol values were significantly elevated to approximately 220 compared with 35.0 ng ml-1 in cortisol implanted vs. sham trout. No significant differences were found between any of the experimental groups for either the affinity (Kd) or maximal number of binding sites (Bmax) for either receptor type. Epinephrine significantly stimulated glucose release from hepatocytes isolated from sham injected trout, but not from cortisol-treated fish. Epinephrine-induced glucose release was blocked by both - and -antagonists. These studies do not support the hypothesis that rainbow trout exposed to chronic cortisol alter properties of hepatic adrenoceptors. 相似文献