红汁乳菇及人工合成菌根的形态结构特征   总被引：1，自引：0，他引：1  

谭著明  张志光  卜晓英  傅绍春  张平 《食用菌学报》2006,13(2):36-44

菌根性真菌子实体、担孢子、菌丝体的形态及与宿主植物形成的菌根的结构是鉴别菌根菌种类的重要特征。AGERER[1]详细描述了Piceirhiza internicrassihyphis与挪威云杉(Picea abies)形成的菌根的显微特征,指出其菌丝套内层有厚壁菌丝,且其中一些菌丝呈平行生长。WANG等[2,3]研究  相似文献   

Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel   总被引：1，自引：0，他引：1  

Haruhiko?ToyoharaEmail author  Masatomi?Hosoi  Isao?Hayashi  Satoshi?Kubota  Hisashi?Hashimoto  Yoshihiro?Yokoyama 《Fisheries Science》2005,71(2):327-332

Expression of HSP70 in response to heat-shock was investigated at the protein and mRNA levels in Mediterranean blue mussel. Western and Northern blot analyses revealed that HSP70 was expressed following heat-shock in the mantle at both protein and mRNA levels, suggesting that gene expression of HSP70 is implicated in the cellular response to heat-shock stress in mussel. It was then attempted to clone HSP70 cDNA in order to determine the primary structure of mussel HSP70. As a result, two full-length cDNA encoding HSP70 were isolated from a cDNA library prepared from the heat-shocked mantle. The isolated cDNA consist of single open reading frames of 2067 bp and 1911 bp which encode proteins of 689 amino acids and 637 amino acids, respectively. Both HSP70 cDNA encode an ATPase do main, and a substrate-binding do main in addition to a Glu-Glu-Val-Asp (EEVD) peptide motif that is specific for cytosolic HSP70. These findings suggest that the cDNA clones obtained in the present study encode cytosolic HSP70.  相似文献   

Properties of scallop mantle collagen: its content,tissue distribution and thermal behavior     

Shoshi Mizuta  Yuko Nakanishi  Masashi Shiraishi  Yoshihiro Yokoyama  Reiji Yoshinaka 《Fisheries Science》2007,73(6):1353-1361

ABSTRACT:   To obtain fundamental information for the effective use of scallop Patinopecten yessoensis mantle, which is one of the underutilized marine resources. Some properties of collagen contained in the mantle were examined by chemical and histochemical techniques. Collagen content in the mantle varied annually, ranging from 0.98 to 1.72% of wet tissue, 7.7 to 12.6% of dry tissue and 13.5 to 26.5% of total protein, being relatively in high level of collagen content of invertebrate muscles. Collagen fiber was densely distributed in the inner connective tissue matrix of the mantle pallial, in contrast to the inner fold part which was rich in muscle fibers. The collagen contained in the crude collagen fraction (residue after alkali extraction), prepared from the mantle, was revealed to have considerably low solubility on hot-water extraction, constantly less than 20% of the total collagen at the temperatures in the range of 20–90°C.  相似文献   

Characterization of the quantitatively major collagen in the mantle of oyster <Emphasis Type="Italic">Crassostrea gigas</Emphasis>     

Shoshi?MizutaEmail author  Tomoyuki?Miyagi  Reiji?Yoshinaka 《Fisheries Science》2005,71(1):229-235

ABSTRACT:   A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent α components (α1 and α2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the α1 and α2 components in 2 : 1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (α1)₂α2.  相似文献   

Culture of mantle epithelial cells expressing shell matrix proteins from scallop Patinopecten yessoensis     

Mayumi Endoh  Yasushi Hasegawa 《Fisheries Science》2006,72(6):1277-1285

ABSTRACT:   In molluscs, mantle epithelial cells secrete organic matrix proteins to form shells. In this study, we established a culture of mantle epithelial cells by using the mantle pallial layer of scallops. We aimed to identify the mantle epithelial cells expressing scallop shell matrix proteins and establish a culture system of epithelial cells. After the mantle pallial layer was carefully isolated from the mantle tissue, explant culture was performed at 4°C. Most cells that migrated from the explant tissue were round cells. Most of the adhered cells retained round morphology, while some of the cells adhered to the dish and showed morphology similar to that of epithelial-like and fibroblast-like cells. When the cultured cells were immunostained with a polyclonal antibody against the shell matrix protein, the antibody recognized many of the adhered cells. An estimation of the number of epithelial cells revealed that approximately 70% of the adhered cells were epithelial cells. This is the first report to describe epithelial cells in cultured mantle cells, which express shell matrix proteins. This culture system may be a useful method for characterization of the mantle epithelial cells.  相似文献   

陇东黄花菜越冬覆盖保墒增收机理研究     

郭海英  杨兴国  张谋草  黄斌  王宁珍 《华北农学报》2006,21(Z2):137-142

通过对庆阳优质特色产品黄花菜越冬期进行地膜覆盖和玉米秸秆覆盖,分析越冬期土壤水分损耗、早春地温变化特征,以及不同覆盖材料水、热资源对黄花菜发育期、生长量、产量构成要素的影响。结论认为:在秋季降水充沛、土壤收墒充足、冬季气温偏高、降水明显偏少的年型,采取越冬覆盖能最大限度保持土壤水库蓄水。陇东塬区黄花菜越冬田间覆盖应大力提倡秸秆覆盖,由于秸秆覆盖既环保,又保墒,而且能有效调节黄花菜发育进程,使产量形成期避开陇东春旱最严重时期,提高水、肥利用率,增产显著。6月中、下旬和7月上、中旬降水对陇东塬黄花菜产量形成至关重要。  相似文献   

覆盖在立体绿化中对植物节水效益影响的研究   总被引：3，自引：0，他引：3  

屈志强  丁国栋  赵方莹  杨莉  牛兰兰 《水土保持研究》2006,13(6):288-289,292

立体绿化是一种投资少、见效快,特别对立交桥能起到很好的绿化效果的绿化形式。但是立交桥区绿化养护困难,尤其是水分补充。通过对比实验证明使用覆盖技术可以明显的增强基质的保水能力,从而起到节水作用,同时减少人工补水工作量。从节水效率讲覆膜的高于覆盖树皮,覆盖树皮高于覆盖石子。  相似文献   

黄土丘陵区不同土壤地类造林树种选择与配置技术研究   总被引：2，自引：0，他引：2  

赵麦换  曹世雄等 《水土保持学报》2002,16(3):43-46

通过 5年实验研究 ,观测了杨树、沙棘、柏树、刺槐、元宝枫纯林和沙棘与杨树、沙棘与柏树、刺槐与柠条的混交林在风化岩沫土土壤、红胶泥土壤、黄绵土土壤上的成活率、年枝条生长量、5 a林冠面积、5 a郁闭度 ,分析得出了黄土丘陵区不同土壤类型造林树种选择和配置的模式。结果表明 :黄土丘陵区造林常见树种及其混交形式在不同土壤类型上呈现不同结果 ,风化岩沫土适合柏树纯林和沙棘与杨树混交、沙棘与柏树混交 ,红胶泥土壤最佳树种为沙棘、刺槐纯林及沙棘与杨树混交 (阴坡 )、沙棘与柏树混交、刺槐与柠条混交 ,而刺槐、柠条纯林和沙棘与杨树混交、沙棘与柏树混交、刺槐与柠条混交适合于黄绵土土壤。此外 ,在各种土壤类型上混交林都优于纯林 ,建议黄土丘陵区造林应乔灌混交为主。该项研究技术在延安市山川秀美工程中推广 ,效果明显 ,建议在黄土丘陵区推广该技术。  相似文献   

BDE209胁迫对翡翠贻贝（Perna viridis）SOD、MDA和GSH的影响   总被引：1，自引：0，他引：1  

黄志斐  张喆  马胜伟  王贺威  巩秀玉  蔡文贵  陈海刚  贾晓平 《农业环境科学学报》2012,31(6):1053-1059

采用半静态实验方法,研究不同浓度BDE209(0.1、1、10mg·L-1)在胁迫1、3、7、15d和清洁海水中释放3、7d后,翡翠贻贝(Perna viridis)外套膜和内脏团超氧化物歧化酶(Superoxide Dismutase,SOD)活性、丙二醛(Malondialdehyde,MDA)含量和谷胱甘肽(Glutathione,GSH)含量的变化规律。结果表明,BDE209胁迫后,翡翠贻贝2组织SOD活性先是受到明显的诱导作用,之后随胁迫时间延长被显著性抑制(P<0.01);BDE209对翡翠贻贝外套膜MDA含量的影响呈诱导-抑制反复变化,对内脏团MDA含量的影响表现为明显的诱导效应,其中胁迫第3d时的诱导率最高,为22.83%。翡翠贻贝外套膜GSH含量在BDE209胁迫初期就被显著诱导(P<0.01),随暴露时间延长诱导效应降低,在第15d时被显著性抑制(P<0.05);BDE209可诱导内脏团GSH含量显著性增加(P<0.05),但随暴露浓度增加GSH含量明显降低。清水恢复阶段,在清洁海水中恢复3d后,翡翠贻贝2组织MDA含量和GSH含量相对于对照组仍有显著性增加(P<0.05);而SOD活性总体在外套膜中受到抑制、内脏团中受到诱导,仅个别浓度组有所恢复。  相似文献   

BDE3胁迫对翡翠贻贝（Perna viridis）SOD、MDA和GSH的影响     

黄志斐  马胜伟  张喆  王贺威  巩秀玉  蔡文贵  陈海刚  贾晓平 《南方水产》2012,8(5):25-30

采用半静态试验方法,研究不同质量浓度的一溴联苯醚（BDE3）（1 μg·L-1、10 μg·L-1和100 μg·L-1）胁迫1 d、3 d、7d和15 d且清洁海水释放3 d和7 d后,翡翠贻贝（Perna viridis）外套膜和内脏团超氧化物歧化酶（SOD）活性、丙二醛（MDA）质量摩尔浓度和谷胱甘肽（GSH）质量分数的变化规律。结果表明,BDE3胁迫后低浓度组翡翠贻贝两组织SOD活性均受到诱导（P〈0.01）,诱导率随胁迫时间延长而下降,中、高浓度组外套膜对BDE3响应比内脏团灵敏;BDE3对翡翠贻贝外套膜b（MDA）的影响总体呈诱导后抑制趋势,对内脏团b（MDA）的影响表现抑制-诱导反复变化,其中低浓度组第3天时诱导率最高（26.04%）,高浓度组第7天时抑制率最高（14.92%）;翡翠贻贝外套膜w（GSH）在（BDE3）迫下,低浓度组一直被诱导（P〈0.01）,中、高浓度组总体呈诱导后抑制作用,内脏团中高浓度组w（GSH）胁迫第1天受到显著抑制（P〈0.05）,随暴露时间的延长,低浓度组w（GSH）显著增加（P〈0.01）,中、高浓度组出现诱导抑制的不规律变化。释放阶段结束后仅个别浓度组恢复至对照组水平。  相似文献